Wocny05tskcqpcpyewgo screen shot 2016 04 20 at 5.01.32 pm

Vectalys

Toulouse, FR

Vectalys is a service biotech company expert in gene delivery, providing full services from expression system design to animal model creation through high quality lentiviral vectors production. Vectalys was founded in 2005 and has now 20 full-time employees exclusively dedicated on designing cellular models, molecular biology and viral vector production and purification.

Vectalys platform is based on retroviral (RV), lentiviral (LV) vectors that efficiently introduce human gene sequences into a wide variety of human cells to knock-in or knock-down specific proteins.

Gene transfer opens the way to develop cell and animal models by transferring target genes into functional cells and tissues. Because primary cells are non permissive towards classical gene transfer technologies, viral based vectors derived from lentiviruses are the only efficient technology to achieve high expression of genes or silencing... Show more »

Vectalys is a service biotech company expert in gene delivery, providing full services from expression system design to animal model creation through high quality lentiviral vectors production. Vectalys was founded in 2005 and has now 20 full-time employees exclusively dedicated on designing cellular models, molecular biology and viral vector production and purification.

Vectalys platform is based on retroviral (RV), lentiviral (LV) vectors that efficiently introduce human gene sequences into a wide variety of human cells to knock-in or knock-down specific proteins.

Gene transfer opens the way to develop cell and animal models by transferring target genes into functional cells and tissues. Because primary cells are non permissive towards classical gene transfer technologies, viral based vectors derived from lentiviruses are the only efficient technology to achieve high expression of genes or silencing sequences in cells or tissues.

Vectalys is a gene delivery platform providing viral recombinant vectors for:
• gene silencing
• gene over-expression
• cell-based assay development
• protein expression and production
• Preclinical trials for gene therapy
• creation of animal models

« Show less

Vectalys has not listed any services.

Ask A Question Find what you're looking for? If not, you can ask this lab a question:
Stable Cell Line Generation
Price on request

GENERATION OF STABLE CELL LINE USING LENTIVIRAL VECTORS

Vectalys provides a custom stable cell line generation service for many applications. The use of lentiviral vectors for stable cell line generation allows for optimal transduction of immortalized, primary and stem cells.

In delicate cells such as hematopoietic... Show more »

GENERATION OF STABLE CELL LINE USING LENTIVIRAL VECTORS

Vectalys provides a custom stable cell line generation service for many applications. The use of lentiviral vectors for stable cell line generation allows for optimal transduction of immortalized, primary and stem cells.

In delicate cells such as hematopoietic lineages for example, classical transfection protocols are not only transient and inefficient, they also induce cytotoxicity effects and proliferative arrests. Gene transfer using highly concentrated and purified lentiviral vectors allows for efficient and safe transduction of such cells.

Lentiviral vectors : a tool of choice for cell engineering

Lentiviral vectors are the engineering tool of choice to study and engineer primary cells without interfering with the cell phenotype, and are also the only tool capable of transducing primary cells without toxicity.

Additionally, the advantages of lentiviral vectors combined with our new technology LentiFlash allow for a faster expression without genomic traces in the target primary cells.

This make Vectalys’ lentiviral vectors the ideal tools for gene editing, cell differentiation, and immunotherapy.

CUSTOM STABLE CELL LINES

Vectalys generates customized stable cell lines from immortalized to primary and stem cells.
Since the creation of the company in 2005, Vectalys has ran more than 1200 cell line generation projects using highly pure and concentrated lentiviral vectors. When considering gene transfer into mammalian cells, the purification level of lentiviral vectors is critical. Vectalys has developed several specific purification levels according to the target cells.

Maintain the original cell phenotype

Using highly pure lentiviral vectors, Vectalys provides stable cell lines while maintaining the original phenotype to mimic, as much as possible, natural in vivo conditions. For example, transduced T cells exhibit an original cell phenotype without any changes to T cells specific marker expression such as CD44, CD69, CD25 and Ly-6C, indicating that the use of our highly pure lentiviral vectors does not impact the T cells’ phenotype.

Manage and control expression level

Cell lines are commonly used for assay development and screening. One of the issues using such cell lines is controlling transgene expression. Being able to fully characterize expression rate and the number of copies in cells is essential to design robust screening assays.

In classical transfection approaches, the challenge is to ensure an appropriate and controlled expression level in target cells for any transgene. Thanks to high quality lentiviral vectors, Vectalys offers the opportunity to observe a quantitative effect linked to a differential gene expression level, by using a controlled Multiplicity of Infection (MOI).

Eliminate the need for antibiotic resistance

Antibiotics can influence the physiology of your target cells which is harmful when working with primary cells to maintain accurate natural in vivo conditions. Because it is possible to achieve 100% of transduced cells in only one experiment by using lentiviral vectors, the use of selection markers such as antibiotics is not required.

Save time and cost

Most of the transfection issues are about gene transfer efficiency into hard to transfect cells (lymphoid and myeloid cell lines, primary and stem cells). Using lentiviral vectors, the expression of the sequence of interest (cDNA, shRNA, miRNA) is stable thanks to the vectors DNA integration into the host genome, providing a permanent cell line in one experiment. This avoids having to transfect targeted cells multiple times for bioproduction or screening (CHO, HEK…). Lentiviral vectors allow to save time, money and energy.

Custom stable cell lines expressing your gene of interest

Obtain your custom stable cell lines with lentiviral vectors dedicated to your target cells. Discover our many possibilities on any cell type:

Immortalized cell lines

  • Human cell lines
  • Mouse cell lines
  • CHO cells

Primary cells

  • Monocytes
  • T/B Lymphocytes
  • Dentritic cells
  • Neurons/Astrocytes
  • Oligodendrocytes
  • Endothelial cells
  • Tumoral cells
  • Hepatocytes
  • Muscle cells

Stem cells

  • Hematopoitetic SC
  • Mesenchymal SC
  • iPS cells
  • ES cells
« Show less
Lentivirus production
Price on request

Lentiviral vectors are time saving tools for stable cell line generation. Using Vectalys Lentiviral vector tools you will be able to get from your gene of interest to the polyclonal cell line generation in a month.

The viral vectors provided by Vectalys, by virtue of their high titre and purity, minimize the deleterious... Show more »

Lentiviral vectors are time saving tools for stable cell line generation. Using Vectalys Lentiviral vector tools you will be able to get from your gene of interest to the polyclonal cell line generation in a month.

The viral vectors provided by Vectalys, by virtue of their high titre and purity, minimize the deleterious phenotypic changes that typically occur following transduction of target cells, such as loss of a sub-populations of transduced cells, and effects on proliferation, viability and differentiation of transduced cells.

Benefits of Vectalys lentiviral vectors
Vectors produced by Vectalys present 3 main advantages based on the absence of serum and on a reliable titer that:
* Ensure that the integration events occurs in every cells at a specific MOI– 100% transduction efficiency
* Enable management and control of the M.O.I. (dose effect)
* Eliminate needs for antibiotic resistance selection steps

Vectalys specific serum free purified vectors batches (C) for primary cells transduction show several advantages:
* The Vectalys patented lentiviral vectors do not affect the viability and the proliferation of your target cells
* Vectalys lentiviral vectors enable to manage both % of positive cells and gene of interest (G.O.I.) level expression by controlling the M.O.I.
* Vectalys lentiviral vectors ensure that all the target cells are genetically modified – 100% transduction efficiency on primary cells and stem cells.
* Vectalys lentiviral vectors eliminate needs for antibiotic resistance selection steps

Vectalys lentiviral vectors for in vivo models

In vivo, lentiviral vectors are efficient tools for noninvasive analysis of tumor animal models. They can be used through direct injection into adult animals or through in vitro transductions of any tumoral cells subsequently reimplanted in the context of cancer models.

Lentiviral vectors have to be highly concentrated and purified in order to achieve an effective transgene expression while maintaining the cells original phenotype, proliferation and viability. These criteria for the development of trustworthy and predictive cancer models are fulfilled by the properties of our lentiviral vectors, building a bridge between in vitro assays, in vivo results and finally therapeutic applications.

« Show less
Mammalian Cell Culture
Price on request
Request a quote for more information about this service.
Microbiology, Virology, and Parasitology
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.
Cell Lines
Price on request
Request a quote for more information about this service.
Cell-Based Assays
Price on request
Request a quote for more information about this service.
Animal Models and Studies
Price on request
Request a quote for more information about this service.
Viral Production and Assays
Price on request
Request a quote for more information about this service.
Cell and Tissue Culture
Price on request
Request a quote for more information about this service.
Cells and Tissues
Price on request
Request a quote for more information about this service.
Virus production
Price on request
Request a quote for more information about this service.

Vectalys has not received any ratings.

Be the first to endorse Vectalys

Endorse this lab