1351380176147 305eacb928c8ce259799993ae74f1b6b

USC Flow Cytometry Core

Los Angeles, California, US

The USC Flow Cytometry Core Facility offers our extended research community with the technology to selectively analyze and sort specific populations of cells up to Biosafety Level 2 (BSL-2).

The USC Flow Core maintains the following devices:

Two analytic cytometers (LSR II, CyAn)

Two cell sorting cytometers (Aria I, Aria II)

One plate imaging system (Cellomics)

Available laser lines include:

UV (355nm)
Violet (405nm)
Blue (488nm)
Red (633nm)

*Both Aria I/II cell sorters are contained in state of the art BioSero Class II Type B Biosafety Cabinets.

The USC Flow Cytometry Core has been used by prominent scientists in Southern California to address questions in adult and embryonic stem cell research, regenerative medicine, cellular immunology, cell biology, chemical carcinogenesis, radiology, human tumor cell cycle kinetics, drug efficacy trials, and in characterizing the... Show more »

The USC Flow Cytometry Core Facility offers our extended research community with the technology to selectively analyze and sort specific populations of cells up to Biosafety Level 2 (BSL-2).

The USC Flow Core maintains the following devices:

Two analytic cytometers (LSR II, CyAn)

Two cell sorting cytometers (Aria I, Aria II)

One plate imaging system (Cellomics)

Available laser lines include:

UV (355nm)
Violet (405nm)
Blue (488nm)
Red (633nm)

*Both Aria I/II cell sorters are contained in state of the art BioSero Class II Type B Biosafety Cabinets.

The USC Flow Cytometry Core has been used by prominent scientists in Southern California to address questions in adult and embryonic stem cell research, regenerative medicine, cellular immunology, cell biology, chemical carcinogenesis, radiology, human tumor cell cycle kinetics, drug efficacy trials, and in characterizing the differences between blood samples between patients and healthy donors.

Cell Sorting & Analysis:
Monday through Friday 10am - 8pm

Consultations:
Monday through Friday 11am - 4pm

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USC Flow Cytometry Core has not listed any services.

Project and Process Management
Starting at $100.00 per hour

General to advanced consultation for investigators seeking to explore novel and creative project designs & possibilities.

General to advanced consultation for investigators seeking to explore novel and creative project designs & possibilities.

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FACS
Fluorescence Activated Cell Sorting
Starting at $200.00 per hour

FACS sorting of specific populations of cells using the Aria I or Aria II cell sorters.
Both sorters use sterile, autoclaved 10% PBS as sheath fluid buffer.
Samples can be maintained at 4°- 42° C both pre and post-sort.
All samples are sorted inside BioSero Class II Type B Biosafety Cabinets.

*Samples must be received in... Show more »

FACS sorting of specific populations of cells using the Aria I or Aria II cell sorters.
Both sorters use sterile, autoclaved 10% PBS as sheath fluid buffer.
Samples can be maintained at 4°- 42° C both pre and post-sort.
All samples are sorted inside BioSero Class II Type B Biosafety Cabinets.

*Samples must be received in 1.7ml microcentrifuge tubes, 5ml (12x75), or 15ml falcon-style tubes.

Four Nozzle Sizes:

70um
85um
100um
135um

Four laser lines available:

UV (355nm)
Violet (405nm)
Blue (488nm)
Red (633nm)

Multiple Collection Options:

1.7ml microcentrifuge/eppendorf tubes
5ml tubes
15ml tubes
Petri Dishes
Glass Slides (frosted/standard)
Ampligrid Plates

(BioRad/Falcon/Other Brands)
6-well plates
12-well plates
24-well plates
48-well plates
96-well plates
384-well plates

*We can customize sorting & cell deposition for nearly every plate on the market. Please inquire prior to service request.

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Flow Cytometry
Starting at $150.00 per hour

General FACS Analysis

General FACS Analysis

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Flow Cytometry Data acquisition
Starting at $150.00 per hour

Acquisition of data on our LSR II analyzer equipped with three excitation laser lines:

UV (355nm)
Blue (488nm)
Red (633nm)

(or)

Acquisition of data on our CyAn analyzer equipped with two excitation laser lines:

Blue (488nm)
Red (643nm)

Acquisition of data on our LSR II analyzer equipped with three excitation laser lines:

UV (355nm)
Blue (488nm)
Red (633nm)

(or)

Acquisition of data on our CyAn analyzer equipped with two excitation laser lines:

Blue (488nm)
Red (643nm)

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Flow Cytometry Data Analysis
Starting at $150.00 per hour

In-depth analysis of FACS data using the latest versions of FlowJo or ModFit (FCS file versions 2.0 or 3.0).

In-depth analysis of FACS data using the latest versions of FlowJo or ModFit (FCS file versions 2.0 or 3.0).

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Flow Cytometry Experimental Design
Starting at $100.00 per hour

Protocol design, staining guidelines & project development:

Preliminary to advanced consultations for investigators interested in starting new projects and/or expanding upon existing projects.

Protocol design, staining guidelines & project development:

Preliminary to advanced consultations for investigators interested in starting new projects and/or expanding upon existing projects.

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Live Cell Imaging
Starting at $150.00 per hour

Digitally image cell size, shape & fluorescence emission for whole samples and/or specific cell subsets on plates or wells. The Cellomics/Arrayscan is a modular, flexible imaging system designed for high-capacity, automated, quantitative cellular and sub-cellular analysis in fixed and live cell formats.

Digitally image cell size, shape & fluorescence emission for whole samples and/or specific cell subsets on plates or wells. The Cellomics/Arrayscan is a modular, flexible imaging system designed for high-capacity, automated, quantitative cellular and sub-cellular analysis in fixed and live cell formats.

  • Analyze whole organisms, tissues, or cells.
  • Superior image-to-answer at 4mins per 96 well plate.
  • Automated objective changer contains 5X Fluar, 10X EC Plan NeoFluar, 10X Plan Apochromat, and 20X LD Plan-Neofluar, allowing for control over optical resolution and field of view
  • Automated excitation filter wheel and automated dichroic/emission cube turret provide efficient automated imaging of combinations of dyes covering blues (e.g. Hoechst), greens (e.g. FITC, GFP), orange reds (e.g. TRITC), deep reds (e.g. Texas Red), and long reds (e.g. Cy5)
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Experimental Design
Price on request
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Microscopy
Price on request
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Cytometry
Price on request
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Cells and Tissues
Price on request
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Data Services
Price on request
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Optical Microscopy
Price on request
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Biology
Price on request
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Imaging & Spectroscopy
Price on request
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Computational Modeling
Price on request
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