The USC Flow Cytometry Core Facility offers our extended research community with the technology to selectively analyze and sort specific populations of cells up to Biosafety Level 2 (BSL-2).
The USC Flow Core maintains the following devices:
Two analytic cytometers (LSR II, CyAn)
Two cell sorting cytometers (Aria I, Aria II)
One plate imaging system (Cellomics)
Available laser lines include:
*Both Aria I/II cell sorters are contained in state of the art BioSero Class II Type B Biosafety Cabinets.
The USC Flow Cytometry Core has been used by prominent scientists in Southern California to address questions in adult and embryonic stem cell research, regenerative medicine, cellular immunology, cell biology, chemical carcinogenesis, radiology, human tumor cell cycle kinetics, drug efficacy trials, and in characterizing the differences between blood samples between patients and healthy donors.
Cell Sorting & Analysis:
Monday through Friday 10am - 8pm
Monday through Friday 11am - 4pm
General to advanced consultation for investigators seeking to explore novel and creative project designs & possibilities.
FACS sorting of specific populations of cells using the Aria I or Aria II cell sorters.
Both sorters use sterile, autoclaved 10% PBS as sheath fluid buffer.
Samples can be maintained at 4°- 42° C both pre and post-sort.
All samples are sorted inside BioSero Class II Type B Biosafety Cabinets.
*Samples must be received in 1.7ml microcentrifuge tubes, 5ml (12x75), or 15ml falcon-style tubes.
Four Nozzle Sizes:
Four laser lines available:
Multiple Collection Options:
1.7ml microcentrifuge/eppendorf tubes
Glass Slides (frosted/standard)
*We can customize sorting & cell deposition for nearly every plate on the market. Please inquire prior to service request.
Acquisition of data on our LSR II analyzer equipped with three excitation laser lines:
Acquisition of data on our CyAn analyzer equipped with two excitation laser lines:
In-depth analysis of FACS data using the latest versions of FlowJo or ModFit (FCS file versions 2.0 or 3.0).
Protocol design, staining guidelines & project development:
Preliminary to advanced consultations for investigators interested in starting new projects and/or expanding upon existing projects.
Digitally image cell size, shape & fluorescence emission for whole samples and/or specific cell subsets on plates or wells. The Cellomics/Arrayscan is a modular, flexible imaging system designed for high-capacity, automated, quantitative cellular and sub-cellular analysis in fixed and live cell formats.
USC Flow Cytometry Core has not received any reviews.
USC Flow Cytometry Core has not received any endorsements.