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University of North Carolina at Chapel Hill School of Medicine

Chapel Hill, North Carolina
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UNC Metabolomics Laboratory

Durham, North Carolina
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The UNC Metabolomics Laboratory is a collaborative resource for global and targeted metabolite profiling from a wide range of biological samples.

The lab is equipped with high-throughput robotic systems for the analysis of large samples sets.

Data analysis is... Show more »

The UNC Metabolomics Laboratory is a collaborative resource for global and targeted metabolite profiling from a wide range of biological samples.

The lab is equipped with high-throughput robotic systems for the analysis of large samples sets.

Data analysis is performed using a number of commercial and home build software tools for the discovery of biomarkers and metabolic signatures of disease, toxicity or other biological outcomes.

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Microbiome Core Facility

Chapel Hill, North Carolina
  • 100% positive reviews
  • 8 Projects Completed
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The human body is the home to approximately 10,000,000,000 bacterial cells that inhabit our gastrointestinal tract, mouth, urogenital tract, and our skin. Most of these bacteria are uncultivable, which meant in the past that researchers were unable to study and characterize them. The... Show more »

The human body is the home to approximately 10,000,000,000 bacterial cells that inhabit our gastrointestinal tract, mouth, urogenital tract, and our skin. Most of these bacteria are uncultivable, which meant in the past that researchers were unable to study and characterize them. The development of metagenomic technologies allow us today to examine microbial communities through culture independent techniques, of which the most used are next-generation sequencing methods.

The UNC Microbiome Core Facility was established to provide technical and research support to study community structure and function of host-associated microbial populations.

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Flow Cytometry Core Facility

Chapel Hill, North Carolina
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The UNC Flow Cytometry Facility provides state-of-the-art flow cytometry and related services to the entire UNC-CH research community as well as to others in the Research Triangle Park area.

A skilled staff provides help with instrument setup, data analysis, and consultation... Show more »

The UNC Flow Cytometry Facility provides state-of-the-art flow cytometry and related services to the entire UNC-CH research community as well as to others in the Research Triangle Park area.

A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves at reduced cost.

A major part of our mission is to teach this technology to investigators, students, and staff.

Please do not hesitate to contact us if you have any questions about flow cytometry, if you want to know if you can use it in your research, how to design experiments, prepare samples, or how to analyze your data.

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Animal Models Core

Chapel Hill, North Carolina
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The UNC Animal Models Core Facility provides the full range of mouse genetic modification services, reagents and related technologies. The Core's services are available to both UNC and Non-UNC clients.

Transgenic (pronuclear injection) animals can be generated in C57BL/6,... Show more »

The UNC Animal Models Core Facility provides the full range of mouse genetic modification services, reagents and related technologies. The Core's services are available to both UNC and Non-UNC clients.

Transgenic (pronuclear injection) animals can be generated in C57BL/6, FVB or other strains. Services for creating transgenic mice include transgene design, construct generation, isolation of the transgene fragment, pronuclear microinjection, founder genotyping and breeding.

The core also offers the full suite of services for BAC (bacterial artificial chromosome) recombineering and microinjection of BAC transgenes.

Gene targeting services are available in ES cells from C57BL/6, 129P2/Ola or BALB/c strains. Other strains available upon request. Gene targeting services include design and cloning of targeting vectors, creating gene-targeted ES cells, blastocyst microinjection of the ES cells to create transmitting chimeras and chimera breeding for germline transmission.

New in 2013: we now offer genome modification services using CRISPR/Cas nucleases. We can produce and validated the nucleases, then use them to generate knockout or knock-in mice, rats or cell lines.

The core can also provide Southern blot validation and blastocyst microinjection of ES cells from EUCOMM, KOMP or other consortia.

Additional services include Cre- or Flp-mediated excision in ES cells or in vivo, chromosome counting, PCR screening and Southern blotting.

Rat transgenic or knockou/knock-in model production is also available.

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X-ray crystallography is the principal technique used to determine three-dimensional structures of biological macromolecules. The central goal of the UNC Crystallography Core Facility is to provide the support and infrastructure necessary to enable individual investigators of any... Show more »

X-ray crystallography is the principal technique used to determine three-dimensional structures of biological macromolecules. The central goal of the UNC Crystallography Core Facility is to provide the support and infrastructure necessary to enable individual investigators of any experience level to initiate and successfully complete a structural biology project.

The facility is state of the art, offering a variety of services in these three areas: crystallization, x-ray diffraction, structure determination.

The primary mission of the MX Facility is to work with researchers here at UNC, however, we are also able to work with researchers at non-UNC academic institutions and commercial companies.

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The Protein Expression and Purification (PEP) core specializes in the production of pure, functional proteins for structural, biophysical, and biochemical studies. It is specifically designed as a “front-end” interface to other components of the Center for Structural Biology. However,... Show more »

The Protein Expression and Purification (PEP) core specializes in the production of pure, functional proteins for structural, biophysical, and biochemical studies. It is specifically designed as a “front-end” interface to other components of the Center for Structural Biology. However, the ability to produce and purify milligram amount of protein enables a broad array of applications and research that extend far beyond the bounds of structural biology, as protein reagents are essential to most areas of biomedical research.

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Antibody Core Facility

Chapel Hill, North Carolina
  • 100% positive reviews
  • 1 Project Completed
  • No endorsements

The primary objective of our facility is to produce monoclonal antibody-producing hybridoma cell lines for UNC investigators. The core develops monoclonal antibodies in mice, rats and hamsters. The core can assist in the screening of hybridomas of interest by ELISA or flow cytometry.... Show more »

The primary objective of our facility is to produce monoclonal antibody-producing hybridoma cell lines for UNC investigators. The core develops monoclonal antibodies in mice, rats and hamsters. The core can assist in the screening of hybridomas of interest by ELISA or flow cytometry. The facility can also produce large amounts of crude antibody in vitro for investigators as well as purify both polyclonal and monoclonal antibodies from tissue culture media or ascites.

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Center for Structural Biology

Chapel Hill, North Carolina
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The CSB provides an integrated platform of expertise, infrastructure and education to foster a wider use of structural biology by the UNC community.

The CSB mission is to make structural biology available as a general tool to any researcher.

Structures of macromolecules... Show more »

The CSB provides an integrated platform of expertise, infrastructure and education to foster a wider use of structural biology by the UNC community.

The CSB mission is to make structural biology available as a general tool to any researcher.

Structures of macromolecules at atomic resolution have helped to revolutionize modern biology. Structural insight is often crucial for understanding the functions of biological macromolecules, including their interactions with small ligands (such as substrates, inhibitors, drugs) and other macromolecules (nucleic acids and/or proteins). Consequently, structural biology represents one of the pillars of basic biomedical research; it is indispensable for deciphering the details of life processes and for understanding the differences between natural and diseased states. Building on such insights, structural biology can assist in devising novel therapies, e.g., through structure-based or structure-aided drug design.

The enormous information content available in high-resolution, three-dimensional structures has sparked the desire of non-structural biologists to examine the structures of the macromolecules that they study.

Structural biology is technically demanding, and it requires highly specialized expertise. The CSB removes or reduces these barriers that usually prevent general researchers from engaging in structural biology-based projects by themselves.

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Tissue Culture Facility

Chapel Hill, North Carolina
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The TCF currently occupies approximately 5000 net square feet (nsf) of laboratory space, not including warm room, cold room, or the specialized microscope space, that consists of six rooms that accommodate media preparation, cell culture manipulations, testing procedures, B-cell... Show more »

The TCF currently occupies approximately 5000 net square feet (nsf) of laboratory space, not including warm room, cold room, or the specialized microscope space, that consists of six rooms that accommodate media preparation, cell culture manipulations, testing procedures, B-cell immortalization services, or other activities that must be functionally separated. A walk-in incubator and walk-in cold storage room are both dedicated to TCF functions. A main administrative area serves as the pick-up area for users to obtain research materials, place orders, or consult with TCF staff.

The facility has seven biosafety cabinets, twelve double-chamber CO2 incubators, two automated cell counters, four specialized cell bioreactors, large and small volume centrifuges, and microscopes to accommodate a wide range of cell culture activities. All microscopes in the TCF’s program have high-resolution digital cameras and digital image capture capabilities. The facility has temperature-critical storage space for samples or materials as follows; a +37°C walk-in warm room, a +4°C walk-in cold room, eight –20°C upright freezers, and three –80°C upright freezers. The TCF has nine liquid nitrogen storage freezers that accommodate the facility’s general cell repository, long-term liquid nitrogen storage for Cancer Center members, primary and back-up storage for immortalized human lymphoblast cultures generated from clinical based research protocols and other human genetics repository functions. There is also a programmable-rate freezer for the automated cryopreservation of cell cultures for the TCF repository cells and for users’ cultures.

The TCF is well equipped and well staffed to meet the current and future specialized research support requirements of the UNC Lineberger Comprehensive Cancer Center members, the UNC research community in general, and other area research-oriented institutions

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Molecular Neuroscience Core

Chapel Hill, North Carolina
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The UNC Neuroscience Center is an inter-departmental research center at the UNC-Chapel Hill School of Medicine. Our mission is to promote neuroscience research at UNC-Chapel Hill with an emphasis on developmental, genetic, and disease-related processes. Resident scientists of the... Show more »

The UNC Neuroscience Center is an inter-departmental research center at the UNC-Chapel Hill School of Medicine. Our mission is to promote neuroscience research at UNC-Chapel Hill with an emphasis on developmental, genetic, and disease-related processes. Resident scientists of the Neuroscience Center are housed in laboratories on the 7th and 8th floors of the Neuroscience Research Building on the School of Medicine campus. Additional neuroscience research groups in both basic (Cell Biology & Physiology) and clinical (Neurology and Ophthalmology) departments are also housed in the Neuroscience Research Building. Multiple other investigators at the UNC-School of Medicine and on the main campus are members of the Neuroscience Center and support a weekly seminar series, an annual symposium and an NINDS-funded Center grant.

The UNC Neuroscience Center is organized into Research Focus Areas that represent key areas in the neurosciences. Each focus area includes researchers with both basic and disease-related interests. Indeed, a key aspect of our mission is to facilitate the rapid integration of basic and disease-related research. The goal is to harness advances and use them to alleviate or cure neurological and psychiatric disorders that are so devastating to individuals and their families.

The UNC Neuroscience Center has several internal core facilities to expedite the mission of our researchers. These include a Functional Genomics Core for expression profiling of developmental and disease-related processes, an in situ hybridization core, and an Imaging Core that supports confocal and multiphoton microscopy, a vector construction core for mouse transgenesis and gene targeting, and an embryonic stem cell core.

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CGIBD Advanced Analytics Core

Chapel Hill, North Carolina
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The CGIBD's Advanced Analytics (AA) Core provides high-quality, quantitative solutions for studying proteins, peptides, and other molecules, including mRNA and cDNA. The Core's immunotechnologies (IT) component provides consultation, ordering, and immunoassay services, and... Show more »

The CGIBD's Advanced Analytics (AA) Core provides high-quality, quantitative solutions for studying proteins, peptides, and other molecules, including mRNA and cDNA. The Core's immunotechnologies (IT) component provides consultation, ordering, and immunoassay services, and supports a wide variety of analytes in both traditional ELISA/EIA and xMAP-based multiplex bead assay formats.

The AA Core also offers high-throughput real-time qPCR service on its Fluidigm BioMark HD system, allowing users to simultaneously measure as many as 96 samples for up to 96 genes, and requiring as little as a single cell per sample when used in combination with the Core's C1 single-cell auto prep system.

Whether measuring cytokines or gene expression, the AA core's favorable pricing and unique cost-sharing model allow investigators access to a wide variety of assays and an extensive probe library for a fraction of their list price.

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High Throughput Sequencing Facility

Chapel Hill, North Carolina
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The HTSF is part of the Carolina Center for Genome Sciences (CCGS) and was established in 2008 with funds from the University Cancer Research Fund (UCRF) and the Lineberger Comprehensive Cancer Center (LCCC).

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Immunology Core

Chapel Hill, North Carolina
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The goal of the Core is to support immunologic research related to the pathogenesis, treatment and diagnosis of HIV infection. The experienced staff of the Core address this goal by supporting the research endeavours of CFAR membership with services to characterize phenotypic,... Show more »

The goal of the Core is to support immunologic research related to the pathogenesis, treatment and diagnosis of HIV infection. The experienced staff of the Core address this goal by supporting the research endeavours of CFAR membership with services to characterize phenotypic, functional and immunogenetic attributes of the immune system in HIV infected individuals.

Immunology Core Aims:
- Receive/procure, process, catalogue, store and distribute biospecimens
- Perform phenotypic and functional assessment of the immune system in HIV infected individuals
- Perform multiplex soluble marker testing
- Perform immunogenetic testing
- Provide access to novel instrumentation
- Provide consultation and training

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Michael Hooker Proteomics Center

Raleigh, North Carolina
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The Mission of the UNC Proteomics Core Facility is to provide UNC investigators with state-of-the-art scientific and technical support in modern proteomics. This requires a commitment to developing, implementing, and optimizing cutting-edge methodologies and instrumentation. In... Show more »

The Mission of the UNC Proteomics Core Facility is to provide UNC investigators with state-of-the-art scientific and technical support in modern proteomics. This requires a commitment to developing, implementing, and optimizing cutting-edge methodologies and instrumentation. In addition, we believe a strong commitment to educating UNC researchers in the field of proteomics is important.

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