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University of North Carolina at Chapel Hill

Chapel Hill, North Carolina, US

The UNC Department of Chemistry Mass Spectrometry facility specializes in small molecule analysis. Our services include, but are not limited to, quantitative mass spectrometry, liquid chromatography separations, fragmentation and structural elucidation mass spectrometry, complex... Show more »

The UNC Department of Chemistry Mass Spectrometry facility specializes in small molecule analysis. Our services include, but are not limited to, quantitative mass spectrometry, liquid chromatography separations, fragmentation and structural elucidation mass spectrometry, complex mixture analysis, high resolution and accurate mass analysis, and MALDI ionization. Our facility provides individualized user training for undergraduate, graduate, and post-doctoral scholars in the Department of Chemistry.

Additionally, we aim to provide the highest quality mass spectrometric services possible to members of the UNC Chapel Hill Department of Chemistry, UNC campus community and surrounding Research Triangle area.

There are two possible mechanisms for having samples analyzed in our facility. Samples can be submitted for analysis on a fee for service basis or students can run their own samples, following required training, on our instrumentation.

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UNC Macromolecular Interactions Facility

Chapel Hill North Carolina
Orders Completed
100% Positive

The Macromolecular Interactions Facility (UNC folks fondly call it the "Mac-In-Fac") is a core facility of the University of North Carolina at Chapel Hill. In operation since 1996, it provides instrumentation and resources for biophysical characterization of biological... Show more »

The Macromolecular Interactions Facility (UNC folks fondly call it the "Mac-In-Fac") is a core facility of the University of North Carolina at Chapel Hill. In operation since 1996, it provides instrumentation and resources for biophysical characterization of biological macromolecules and their interactions with cognate ligands. Core instrumentation includes surface plasmon resonance (SPR)-based and bio-layer interferometry (BLI)-based biosensors, isothermal titration calorimeters (ITCs) including the fully-automated Auto-ITC200, a circular dichroism (CD) spectropolarimeter, static and dynamic light scattering instruments, a fluorescence microplate reader, a nano-DSF (nano-differential scanning fluorimeter), an analytical ultracentrifuge, and a spectrofluorometer. Though the facility was established for use by UNC researchers, we welcome researchers from other academic institutions and biotech companies. The service is strictly on first-come-first-served basis

Equipment
- Malvern/MicroCal Auto-iTC200: Fully-Automated Isothermal Titration Calorimeter
- Bio-Rad Proteon XPR36: Surface plasmon resonance (SPR)-based biosensor
- ForteBio Octet Red384: Bio-layer Interferometry (BLI)-based biosensor
- SEC-MALS system: Size Exclusion Column with Wyatt multi-angle light scattering system
- Wyatt DynaPro Dynamic Light Scattering Plate Reader
- Applied Photophysics Chirascan Plus Circular Dichroism (CD) spectrometer
- Nanotemper Prometheus NT.48 nanoDSF
- Beckman XL-I: Analytical Ultracentrifuge
- Malvern/MicroCal VP-ITC: Isothermal Titration Calorimeter
- PHERAstar FS Microplate Reader
- Horiba Jobin Yvon Fluorolog-3 T-format Spectrofluorometer

Publications
To date, close to 400 papers containing data obtained at the Mac-In-Fac have been published.
Here are some recent ones:
- A multi-laboratory Comparision of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation.120 authors including A. Tripathy. PLOS ONE (in press), 2015.
- Discovery of a selective, substrate-competitive inhibitor of the lysine methyltransferase SETD8. Ma A, Yu W, Li F, Bleich RM, Herold JM, Butler KV, Norris JL, Korboukh V, Tripathy A, Janzen WP, Arrowsmith CH, Frye SV, Vedadi M, Brown PJ, Jin J. J Med Chem. 2014 Aug 14;57(15):6822-33.
- The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. Nat Commun. 2014;5:3078.
- Molecular Basis for pH-Dependent Mucosal Dehydration in Cystic Fibrosis Airways. Garland, A.L., Walton, W.G., Coakley, R.D., Tan, C.D., Gilmore, R.C., Hobbs, C.A., Tripathy, A., Clunes, L.A., Bencharit, S., Stutts, M.J., Betts, L., Redinbo, M.R., and Tarran, R. Proceedings of the National Academy of Sciences USA 2013 110 (40) 15973-15978.
- Identification of novel integrin binding partners for CIB1: structural and thermodynamic basis of CIB1 promiscuity. Thomas C Freeman, Justin L Black, Holly G Bray, Onur Dagliyan, Yi I. Wu, Ashutosh Tripathy, Nikolay V. Dokholyan, Tina M Leisner, and Leslie V. Parise. Biochemistry 2013 Sept. DOI: 10.1021/bi400678y.
- Plant tumour biocontrol agent employs a tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase. Chopra, S., Palencia, A., Virus, C., Tripathy, A., Temple, B.R., Velazquez-Campoy, A., Cusack, S., and Reader, J. S Nature Communications 2013 4: 1417.

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Microscopy Services Laboratory

Chapel Hill North Carolina
Order Completed
0% Positive

Our mission is to help you with your research.

We provide training, assistance and services in light microscopy, electron microscopy and image analysis.

We look forward to hearing about your research needs and how we can help. We will strive to understand the details of what... Show more »

Our mission is to help you with your research.

We provide training, assistance and services in light microscopy, electron microscopy and image analysis.

We look forward to hearing about your research needs and how we can help. We will strive to understand the details of what you are doing, advise which instruments will most advance your research, train you on the relevant equipment and provide continued assistance. We are here to help you and can consult on all aspects of your project, from sample preparation, to image acquisition and data analysis.

The MSL is a UNC core facility that is part of the Department of Pathology and Laboratory Medicine. We are also the light microscopy core for the Lineberger Comprehensive Cancer Center.

Selected Publications

  • Hathaway CK, Chang AS, Grant R, Kim HS, Madden VJ, Bagnell CR Jr, Jennette JC, Smithies O, Kakoki M. High Elmo1 expression aggravates and low Elmo1 expression prevents diabetic nephropathy. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2218-22. doi: 10.1073/pnas.1600511113. PubMed PMID: 26858454; PubMed Central PMCID: PMC4776516.
  • Schmid RS, Simon JM, Vitucci M, McNeill RS, Bash RE, Werneke AM, Huey L, White KK, Ewend MG, Wu J, Miller CR. Core pathway mutations induce de-differentiation of murine astrocytes into glioblastoma stem cells that are sensitive to radiation but resistant to temozolomide. Neuro Oncol. 2016 Jul;18(7):962-73. doi: 10.1093/neuonc/nov321. PubMed PMID: 26826202; PubMed Central PMCID: PMC4896545.
  • Hathaway CK, Gasim AM, Grant R, Chang AS, Kim HS, Madden VJ, Bagnell CR Jr, Jennette JC, Smithies O, Kakoki M. Low TGFβ1 expression prevents and high expression exacerbates diabetic nephropathy in mice. Proc Natl Acad Sci U S A. 2015 May 5;112(18):5815-20. doi: 10.1073/pnas.1504777112. PubMed PMID: 25902541; PubMed Central PMCID: PMC4426439.
  • Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M. iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. Cell. 2014 Nov 6;159(4):896-910. doi: 10.1016/j.cell.2014.10.010. PubMed PMID: 25417164.
  • McNeill RS, Schmid RS, Bash RE, Vitucci M, White KK, Werneke AM, Constance BH, Huff B, Miller CR. Modeling astrocytoma pathogenesis in vitro and in vivo using cortical astrocytes or neural stem cells from conditional, genetically engineered mice. J Vis Exp. 2014 Aug 12;(90):e51763. doi: 10.3791/51763. PubMed PMID: 25146643; PubMed Central PMCID: PMC4827968.
  • Ariel P, Hoppa MB, Ryan TA. Intrinsic variability in Pv, RRP size, Ca(2+) channel repertoire, and presynaptic potentiation in individual synaptic boutons. Front Synaptic Neurosci. 2013 Jan 11;4:9. doi: 10.3389/fnsyn.2012.00009. PubMed PMID: 23335896; PubMed Central PMCID: PMC3542534.
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Histology Research Core Facility

Chapel Hill North Carolina
Orders Completed
80% Positive

With the advancement and popular emphasis in molecular techniques in today’s laboratory, anatomy-based research—often critical in defining research projects—is becoming a lost art. Many research laboratories no longer have the floor space nor can afford the specialized histology... Show more »

With the advancement and popular emphasis in molecular techniques in today’s laboratory, anatomy-based research—often critical in defining research projects—is becoming a lost art. Many research laboratories no longer have the floor space nor can afford the specialized histology equipment and the personnel required to perform these complex procedures. Today, researchers frequently include expensive mouse models to support and complement their research projects. Consistent and reliable histology is critical in providing structural and quantitative data for these mouse/animal models. The Departmental Histology Facility offers a variety of services insuring that histological techniques are always available for investigators and their research project needs.

The Histology Facility of the Department of Cell and Molecular Physiology at UNC-Chapel Hill is housed in a 600-square foot research laboratory in the Glaxo Building, room 4. The facility is equipped with two Leica 1950 cryostats, a Cryojane Tape Transfer frozen sectioning system, two Leica 2235 rotary microtomes, microscopes and a Vibratome.

The Facility has the equipment and expertise to produce reliable high-quality sections from fixed and unfixed tissues, frozen and paraffin-embedded tissues. Furthermore we provide a unique serial interrupted sectioning technique. This technique allows analysis of the full length of 4mm vessels or tissues, collected on multiple slides with each slide containing serial sections representing a specific distance through the full length of that tissue. In addition, special stains and immunohistochemical assays are routinely performed in the facility.

Recent Publications

  • Cooley B, Funkhouser W, Monroe D, Ezzell A, Mann DM, Lin FC, Monahan PE, Stafford DW. Prophylactic efficacy of BeneFIX vs Alprolix in hemophilia B mice. Blood. 2016 Apr 22. pii: blood-2016-01-696104. [Epub ahead of print]
  • Cozzo AJ, Sundaram S, Ottavia Zattra O, Qin Y, Freemerman AJ, Essaid L, Darr DB, Montgomery SA, McNaughton KK, Ezzell JA, Galanko JA, Troester MA, Makowski L. cMET inhibitor crizotinib impairs angiogenesis and reduces tumor burden in the C3(1)-Tag model of basal-like breast cancer. Springer Plus Breast Cancer Collection, IN PRESS.
  • Corbin JM, Overcash RF, Wren JD, Coburn A, Tipton GJ, Ezzell JA, McNaughton KK, Fung KM, Kosanke SD, Ruiz-Echevarria MJ. Analysis of TMEFF2 allografts and transgenic mouse models reveals roles in prostate regeneration and cancer. Prostate. 2015 Sep 29. doi: 10.1002/pros.23103. [Epub ahead of print] PMID:26417683
  • Van Landeghem L, Santoro MA, Mah AT, Krebs AE, Dehmer JJ, McNaughton KK, Helmrath MA, Magness ST, Lund PK. IGF1 stimulates crypt expansion via differential activation of 2 intestinal stem cell populations. FASEB J. 2015 Apr 2. pii: fj.14-264010. [Epub ahead of print] PMID:25837582
  • Sundaram S, Freemerman AJ, Galanko JA, McNaughton KK, Bendt KM, Darr DB, Troester MA, Makowski L. Obesity-mediated regulation of HGF/c-Met is associated with reduced basal-like breast cancer latency in parous mice. PLoS One. 2014 Oct 29;9(10):e111394. doi: 10.1371/journal.pone.0111394. eCollection 2014. PMID: 25354395
  • Casbas-Hernandez P, Sun X, Roman-Perez E, D'arcy M, Sandhu R, Hishida A, McNaughton KK, Yang XR, Makowski L, Sherman ME, Figueroa JD, Troester MA. Tumor Intrinsic Subtype is Reflected in Cancer-Adjacent Tissue. Cancer Epidemiol Biomarkers Prev. 2014 Dec 2. pii: cebp.0934.2014. PMID:25465802
  • Sundaram S, Le TL, Essaid L, Freemerman AJ, Huang MJ, Galanko JA, McNaughton KK, Bendt KM, Darr DB, Troester MA, Makowski L. Weight Loss Reversed Obesity-Induced HGF/c-Met Pathway and Basal-Like Breast Cancer Progression. Front Oncol. 2014 Jul 8;4:175. doi: 10.3389/fonc.2014.00175. eCollection 2014. PMID:25072025
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UNC Eshelman School of Pharmacy - NMR Facility

Chapel Hill North Carolina
No Reviews Available

The Eshelman School of Pharmacy NMR Facility at the University of North Carolina at Chapel Hill has had a diverse group of users with a broad range of interests. These include natural products or other "small" molecules; polymers; and biomolecular structure and dynamics. ... Show more »

The Eshelman School of Pharmacy NMR Facility at the University of North Carolina at Chapel Hill has had a diverse group of users with a broad range of interests. These include natural products or other "small" molecules; polymers; and biomolecular structure and dynamics. The Facility has a number of spectrometers, including an Inova 400, which is typically used for routine 1H or 13C one-dimensional experiments, and for those two-dimensional experiments usually associated with "small" molecules; e.g., COSY, ROESY, HSQC, HMBC, and so on. We also have a fully automated 400MR, which is also used for these experiments, as well as for 19F and 31P. In addition, the Laboratory has an Inova 500 which is now often used to study small molecules. However, it is designed for biomolecular NMR, and is therefore our most versatile instrument, with four channels instead of two; waveform generators on all four channels; pre-cooling of the VT air to permit low-temperature studies; and a variety of probes. Recently it has also been used to study more "exotic" nuclei, such as 11B, 119Sn, and129Xe.

Inova 500
- 4 full channels
- short 2H pulse
- NOESY, ROESY, TOCSY…HMQC, HSQC, HMBC…
- triple resonance experiments (1H, 13C, 15N, 31P)
- relaxation experiments
- Probes:
5mm triple resonance XYZ
5mm broadband PFG
5mm Penta PFG
3mm triple resonance PFG

Inova 400
- 1H & 13C detect
- 1H & 13C decouple
- 1D experiments: APT, DEPT, INEPT…
- 2D experiments: NOESY, COSY, DQFC, HSQC, HMBC…

400MR Spectrometer
- 1H, 13C, 31P, 19F experiments
- 2D experiments: NOESY, COSY, DQFC, HSQC, HMBC…
- automated
- SMS 100 sample changer (robot)
- dual ProTune for automatic tuning
- automated locking
- gradient shimming

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Translational Pathology Laboratory (TPL)

Chapel Hill North Carolina
No Reviews Available

The Translational Pathology Laboratory (TPL) is a core facility in the UNC School of Medicine administered by the Lineberger Comprehensive Cancer Center and the Department of Pathology and Laboratory Medicine. We offer access to sophisticated digital pathology platforms and study... Show more »

The Translational Pathology Laboratory (TPL) is a core facility in the UNC School of Medicine administered by the Lineberger Comprehensive Cancer Center and the Department of Pathology and Laboratory Medicine. We offer access to sophisticated digital pathology platforms and study design support to all UNC investigators to enhance translational research.

Recent Publications

  • Karginova O, Siegel MB, Van Swearingen AE, Deal AM, Adamo B, Sambade MJ, et al. Efficacy of Carboplatin Alone and in Combination with ABT888 in Intracranial Murine Models of BRCA-Mutated and BRCA-Wild-Type Triple-Negative Breast Cancer. Mol Cancer Ther. 2015;14(4):920-30. Epub 2015/04/01. doi: 10.1158/1535-7163.MCT-14-0474. PubMed PMID: 25824335; PubMed Central PMCID: PMC4394032.
  • Chang EHea. Palladin is Upregulated in Kidney Disease and Contributes to Epithelial Cell Migration After Injury. Sci Rep. 2015;5(7695).
  • Carson CC, Moschos SJ, Edmiston SN, Darr DB, Nikolaishvili-Feinberg N, Groben PA, et al. IL2 Inducible T-cell Kinase, a Novel Therapeutic Target in Melanoma. Clin Cancer Res. 2015;21(9):2167-76. Epub 2015/05/03. doi: 10.1158/1078-0432.CCR-14-1826. PubMed PMID: 25934889; PubMed Central PMCID: PMC4418029.
  • Zhou B, Damrauer JS, Bailey ST, Hadzic T, Jeong Y, Clark K, et al. Erythropoietin promotes breast tumorigenesis through tumor-initiating cell self-renewal. J Clin Invest. 2014;124(2):553-63. Epub 2014/01/18. doi: 10.1172/JCI69804. PubMed PMID: 24435044; PubMed Central PMCID: PMC3904607.
  • Zhang L YH, Burk LM, Inscoe CR, Hadsell MJ, Chtcheprov P, Lee YZ, Lu J, Chang S, Zhou O. Image-guided microbeam irradiation to brain tumour bearing mice using a carbon nanotube x-ray source array. Phys Med Biol. 2014;59:1283-303. PubMed Central PMCID: PMCPMC4028041.
  • Wyss AB WM, Avery CL, Herring AH, Bensen JT, Barnholtz-Sloan JS, Funkhouser WK, Olshan AF. Single nucleotide polymorphisms in nucleotide excision repair genes, Cancer Treatment, and the Head and Neck Cancer Survival. Cancer Causes & Control 2014;25:437, 2014. PubMed Central PMCID: PMC PMC4096829.
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2017-02-21 09:58:34 -1000

Net Promoter Score of 10 received.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.

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