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University of Nebraska, Lincoln

Lincoln, Nebraska, US
Yac9pavxs5oyil5v6ksk rbc logo winner

Spectroscopy and Biophysics Core

Lincoln Nebraska
130 Orders Completed
95% Positive
1 Endorsement

The role of the Spectroscopy and Biophysics Core is to provide instrumentation, training and support for any type of experimental work involving biophysical and spectroscopic measurements. This include CD spectroscopy, ITC, DSC, stopped flow, fluorescence/chemiluminescence,... Show more »

The role of the Spectroscopy and Biophysics Core is to provide instrumentation, training and support for any type of experimental work involving biophysical and spectroscopic measurements. This include CD spectroscopy, ITC, DSC, stopped flow, fluorescence/chemiluminescence, elemental analysis, metabolomics (targeted small molecule) and proteomics (protein ID, gel extraction, targeted analysis and PTMs).

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Nebraska Center for Materials and Nanoscience

Lincoln Nebraska
No Reviews Available

NCMN Nanofabrication Central Facility provides state-of-the-art instrument for designing, fabricating, characterizing and testing of complex nano/micro-scale structures and devices. All these advanced tool sets are housed within the 4000 sq. ft. clean room at the Voelte-Keegan... Show more »

NCMN Nanofabrication Central Facility provides state-of-the-art instrument for designing, fabricating, characterizing and testing of complex nano/micro-scale structures and devices. All these advanced tool sets are housed within the 4000 sq. ft. clean room at the Voelte-Keegan Nanoscience Research Center. The facility opens to all UNL researchers as well as external (including private sector) researchers for carrying out their research projects in physics, chemistry, nano/microelectronics, MEMs/NEMs, nano-bio, and other related and interdisciplinary areas. Staff support is available for training, process consultation, and collaboration on new process development.

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0d2yujmt0kxd8qfmjw6j extreme light laboratory

Extreme Light Laboratory

Lincoln Nebraska
No Reviews Available

The Extreme Light Core Facility provides laser services to investigators from around the world. The state of the art facility offers scientists access to specialized laser equipment to carry out a broad range of experiments.

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Proteomics and Metabolomics Facility

Lincoln Nebraska
Orders Completed
100% Positive

The Proteomics and Metabolomics Facility (PMF) at the Center for Biotechnology offers a range of technical services using mass spectrometry, including identification and relative quantification of proteins and several advanced methods for profiling and quantitation of small... Show more »

The Proteomics and Metabolomics Facility (PMF) at the Center for Biotechnology offers a range of technical services using mass spectrometry, including identification and relative quantification of proteins and several advanced methods for profiling and quantitation of small molecules. With highly specialized technology capabilities and proficiency and expertise of personnel in the field of mass spectrometry, the facility is well-prepared and positioned to serve as a regional, national, and international resource and to foster collaboration in the fast developing field of proteomics and metabolomics.

Instruments & Facilities

PROTEOMICS PLATFORM

❖ Thermo Q-Exactive – High Field (QE-HF) coupled to Dionex U3000 nanoRSLC

  • Bottom-up proteomics for protein identification, quantification using labeling (TMT- 6 or 10 plex) or label-free approaches; PTMs characterization (phosphorylation, ubiquitination, acetylation, etc.); Intact protein characterization.
  • Targeted quantification of peptides using Parallel Reaction Monitoring (PRM) or Data Independent Acquisition (DIA).
  • High Resolution/Accurate Mass (HR/AM) instrument
  • Resolving power up to 240,000 at 200 m/z
  • Sub ppm mass accuracy
  • Scan speed of 18 Hz at 15,000 resolution
  • Flow rate of the nanopump: 20 nL/min to 1.5 µL/min
  • Pressure to 800 bar (11,600 psi)

❖ Mascot 2.5.1 (Matrix Science Ltd) for protein identification from MS/MS spectra

❖ Proteome Discoverer 2.1 Search Engine (Thermo) for metabolic, isotope labeling and label free quantitative proteomics

❖ Scaffold 4.4.7 (Proteome Software) for protein identification, reporting and label-free quantitative proteomics

❖ PEAKS 7.5 (Bioinformatics Solutions Inc) for de novo sequencing and PTM characterization

METABOLOMICS PLATFORM

❖ Agilent 1290 Infinity II UPLC

  • Separation, profiling and quantification of a wide range of small molecules: PEG, dyes, vitamins, organic acids, carbohydrates, triglycerides, phospholipids, FAME, and more….
  • Peptide and protein fractionation for proteomics applications.
  • Flow rate range: 0.05 to 5 mL/min
  • Pressure 1300 bar (18,800 psi)
  • Multicolumn thermostat and multisampler
  • DAD, ELSD and Fluorescence detector allowing detection of a wide range of compounds (absorbing, fluorescent, non-volatile, and semi-volatile compounds)

❖ Agilent GC-MS 5977A

  • Separation, profiling and quantification of volatile compounds for food regulation, environmental analysis, forensic analysis, drug detection, research.
  • Headspace sampler for analysis of volatile and semi-volatile compounds in solid, liquid and gas samples (without sample extraction)
  • NIST and Fiehn GC-MS compound libraries for identification/confirmation of unknown volatile compounds
  • Integrates with Mass Profiler Professional for qualitative and quantitative studies
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Department of Entomology

No Reviews Available

Insect pest management aims to develop and implement environmentally sound, sustainable practices to reduce the impact of insect pests. This research area concentrates on pests of field crops, livestock, horticultural crops, urban environments, and forests.

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Bioinformatics Core Research Facility

Lincoln Nebraska
No Reviews Available

The UNL Bioinformatics Core Research Facility (BCRF) is located on the City Campus of the University of Nebraska-Lincoln, and is part of the Center for Biotechnology which houses several other cores. We offer rich standard and custom bioinformatics services for a reasonable price... Show more »

The UNL Bioinformatics Core Research Facility (BCRF) is located on the City Campus of the University of Nebraska-Lincoln, and is part of the Center for Biotechnology which houses several other cores. We offer rich standard and custom bioinformatics services for a reasonable price and timely turnaround, and can provide outstanding service from data quality assurance all the way to publication-ready graphs and data deposition to standard repositories.

Our average turnaround time is approximately 7 business days for most services, thanks to our HPC Linux compute cluster and priority-access to the University's supercomputing cluster for larger analyses.

Most recent publication:
Sinha R, Clarke J, Riethoven J. Metabolic Syndrome, Gut Microbiome and Dietary Bioactive Peptides, an Unexplored Triad. Diabesity, v. 4, n. 1, p. 1-4, jan. 2018. ISSN 2369-0569. doi:http://dx.doi.org/10.15562/diabesity.2018.45.

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