**The University of Iowa High Throughput Screening Core facility (UIHTS) is offering in vitro, high-throughput testing for COVID-19 projects. Click the Resources tab or inquire for details.**
Established by the university sponsors and NIH funding, the University of Iowa High Throughput Screening Facility (UIHTS) is a high throughput platform that integrates robotics, detection systems and chemical /biologics libraries. UIHTS enables scalable screening approaches, not only to foster hit and lead generation for drug discovery and development through screening of large chemical/biologics libraries; but also to facilitate molecular probe discovery for mechanism of action (MOA) studies of chemical biology through screening of focused intellectually-designed compound collections. As a university core facility, UIHTS provides highly flexible screening services, project management, grant assistance and assay/technology development for investigators across campus and beyond.
In addition, UIHTS will be serving as your primary source and portal for bridging your high throughput biology and potential translational medical researches by nurturing the collaboration with medicinal chemistry, pharmacokinetics and pharmacodynamics, and clinical trials.
High Throughput Screening: from scalable screen, pilot screen, primary screen, to secondary screen
UIHTS provides scalable screen with defined number of compounds (~50 compounds) for unbiased in vitro analysis for the specific targets or phenotypes
UIHTS provides pilot screen with a small library of Microsource spectrum library (2130 compounds) to generate some preliminary results using optimized assays for the validation of the screen robustness for large library screening.
UIHTS provides primary screen with a structure diversity library of ChemBridge Diversity Set (50K compounds) to generate initial hits as the basis of further hit-to-lead and leads optimization process.
UIHTS can work with you to develop your bench-top assays or your ideas of assays of your biological targets into microplate-based assays which are compatible with high throughput screening. These assays include biochemical assays and cell-based assays. The assay formats include 96-well, 384-well or 1536-well formats with various detection technologies (absorbance, fluorescence, luminescence, fluorescence polarization (FP), TR-FRET, AlphaScreen, and label-free (Epic/EnSpire)).
UIHTS can also work with you on different targets, which include 1) Biochemical assays on enzymes (i.e. kinases, GTPase, and DNA methyltransferase), protein-protein interactions, nucleic acid-protein interactions; 2) Cell-based assays on membrane proteins (i.e. GPCR, ion channels, transporters, and RGP), nuclear proteins, and phenotypic based live-dead cell death.
UIHTS can work with you with high content imaging of both fixed and live cells. Our imaging capability can work with glass slides, 24-wells, 96-well and 1536-wells microplate automatically.
Assay Adaption and Validation
UIHTS has developed a practical approach to transfer/adapt your assays in different stages into high throughput screening compatible assays as scalable screen assays, ready for any scale of screening (from small library of 1-2K compounds, to up to 350K-2 M compounds), both for small molecules and biologics.
UIHTS has also established a protocol for the validation of your assays, either biochemical or cell-based assays. The assays will be validated by the common HTS parameters of Z factors and S/N ratios. If necessary, dose responses of the assays will be determined to check the robustness of the assays, and to confirm the physiological relevance on targeted mechanism of actions
Exemplary Assay Protocols
Contrast to common bench-top assays, scalable and HTS compatile assays need detailed protocol and standard operation procedures (SOPs) for the consistence and reproducibility of the assays. Protocols and SOPs are especially critical for the transfer and adaption of assays among different labs. We recommend that you use the protocols or SOPs in the formats as on PubChem or Nature Protocols. Contact us if you need an example of SOP of UIHTS facility.
University of Iowa High Throughput Screening Core facility (UIHTS) has not received any reviews.
University of Iowa High Throughput Screening Core facility (UIHTS) has not received any endorsements.