The University of California, Los Angeles (UCLA) is a public research university located in the Westwood neighborhood of Los Angeles, California, USA. It is the second oldest of the ten campuses of the University of California system, and the most selective public university in the United States. UCLA, (along with Berkeley), is considered a flagship campus of the University of California system, and offers over 300 undergraduate and graduate degree programs in a wide range of disciplines. [From Wikipedia.org]
The Clinical & Translational Research Laboratory (CTRL) offers a full range of cost-effective laboratory testing to support your research efforts. The laboratory performs routine chemistry tests (basic metabolic panel, comprehensive metabolic group, electrolyte panel, hepatic... Show more »
The Clinical & Translational Research Laboratory (CTRL) offers a full range of cost-effective laboratory testing to support your research efforts. The laboratory performs routine chemistry tests (basic metabolic panel, comprehensive metabolic group, electrolyte panel, hepatic panel, lipid panel and individual tests), thyroid function tests, fertility tests, tumor marker tests, basic coagulation testing and routine urinalysis testing. These tests are performed in the CTRL lab; however, referral tests can be sent to the UCLA Clinical Laboratory or outside reference laboratories. New assays not on the test menu can be validated and performed to meet the needs of individual research investigators.
The laboratory is California-licensed, CLIA-certified, and CAP-accredited. A copy of each certificate can be issued upon request. The lab is staffed by ten research scientists, two hospital laboratory technicians, and two clinical laboratory scientists. It occupies approximately 5,000 square feet of space located on the A-level of the Center for Health Sciences (CHS) building. The facility works with investigators both inside and outside the UCLA enterprise on a collaborative and fee-for-service basis.« Show less
UCLA Clinical Microarray Core/JCCC Genomics Shared Resource (CMC/GSR), directed by Dr. Xinmin Li, including a staff of six with 56 years combined genomics experience. [http://youtu.be/9gEONcrFDDk] We are a pharma trial site for Pfizer and... Show more »
UCLA Clinical Microarray Core/JCCC Genomics Shared Resource (CMC/GSR), directed by Dr. Xinmin Li, including a staff of six with 56 years combined genomics experience. [http://youtu.be/9gEONcrFDDk] We are a pharma trial site for Pfizer and Quintiles. This CLIA/CAP genomic facility equipped with all major next generation sequencing/microarray instruments and related bioinformatics resources including:
Nucleic acids isolation and QC
o MagNa Pure Compact
o Applied Biosystems 7500 Fast Real
o Agilent 2100 Bioanalyzer, NanoDrop 8000, Qubit 2.0 Fluorometer
o Illumina HiSeq2500 sequencing system
o Illumina MiSeq sequencing system
o Ion Personal Genome Machine (PGMTM)
o IntegenX Apollo 324 system
o Fluidigm Access Array™ System
o Affymetrix GeneChip® System 3000Dx v.2
o Affymetrix GeneChip® System 3000
o Affymetrix GeneAtlas system
o Agilent microarray system
o Roche NimbleGen microarray system
o miRNA array system
o Beckman Coulter ArrayPlex
o Hoffman2 data storage server
o Hoffman2 Linus clusters equipped with various genomic data analysis tools
including Galaxy server
o Lab-hosted data analysis server and data analysis tools including Partek
Genomics Suite, NextGENe, Ingenuity.
With the state of the art instruments and well established data analysis and management pipelines, the CMC/GSR offers a wide range of genomic services including:
Automated DNA/RNA isolation & quality evaluation
Next generation sequencing services
o Whole exon sequencing
o Targeted resequencing
Microarray serviceso Gene expression profiling
o SNP genotyping & CNV analysis
o Global methylation analysis
o ChIP-Chip analysis
o miRNA profiling
o NGS/Microarray Data analysis
o Data analysis consultation & software tutorial
o Online data delivery & long-term data storage
These state-of-the-art NGS and microarray services are offered in a cost-effective and timely manner to basic science, translational and clinical researchers.
The CMC/GSR clientele include researchers generally unfamiliar with whole genome and bioinformatics approaches as well as experts seeking more sophisticated solutions.
In addition to providing comprehensive genomic services, the CMC/GSR provides “beyond service” assistance in science and technology which includes fostering new ideas, facilitating project collaboration and integration across biomedical disciplines and developing new applications to advance the quality of the science.
The CMC/GSR also provides educational training to faculty, staff and students and strives to raise awareness of new directions and major discoveries in the areas of genomics and bioinformatics.
By providing the above services, the CMC/GSR has supported a wide range of translational investigations, including:
Evaluation of drug effect
Optimization of drug dose
Identification of drug targets
Identification of expression signature for disease classification
Identification of gene expression markers for disease diagnosis/prognosis
Evaluation of host response to pathogen infection
Identification of disease-associated SNPs
Identification of pathogenic copy number variations
Understanding molecular mechanisms of pathogenesis and evolution
DNA minimum is 1 ng, HIGH quality
RNA Standard protocol - 1 ug
RNA specialized protocol - 500pg
In summary, the CMC/GSR provides next-generation sequencing (NGS)/microarray platforms, an integrated bioinformatics pipeline and technical/intellectual expertise, which has significantly facilitated scientific findings and yielded numerous high impact publications including:
Bensinger SJ, Bradley MN, Joseph SB, Zelcer N, Janssen EM, Hausner MA, Shih R, Parks JS, Edwards PA, Jamieson BD, Tontonoz P. LXR signaling couples sterol metabolism to proliferation in the acquired immune response. Cell. 2008;134:97-111. PMC2626438
Burton JB, Johnson M, Sato M, Koh SB, Mulholland DJ, Stout D, Chatziioannou AF, Phelps ME, Wu H, Wu L. Adenovirus-mediated gene expression imaging to directly
detect sentinel lymph node metastasis of prostate cancer. Nat Med. 2008;14:882-8. PMC2811163
Chang J, Wang Z, Tang E, Fan Z, McCauley L, Franceschi R, Guan K, Krebsbach PH, Wang CY. Inhibition of osteoblastic bone formation by nuclear factor-kappaB.
Nat Med. 2009;15:682-9. PMC2768554 Chin MH, Pellegrini M, Plath K, Lowry WE. Molecular analyses of human induced pluripotent stem cells and embryonic stem cells. Cell Stem Cell. 2010;7:263-9. PMC3276111
Cokus SJ, Feng S, Zhang X, Chen Z, Merriman B, Haudenschild CD, Pradhan S, Nelson SF, Pellegrini M, Jacobsen SE. Shotgun bisulphite sequencing of the
Arabidopsis genome reveals DNA methylation patterning. Nature. 2008;452:215-9. PMC2377394
Fan Z, Yamaza T, Lee JS, Yu J, Wang S, Fan G, Shi S, Wang CY. BCOR regulates mesenchymal stem cell function by epigenetic mechanisms. Nat Cell Biol. 2009;11:1002-9. PMC2752141
Ferrari R, Pellegrini M, Horwitz GA, Xie W, Berk AJ, Kurdistani SK. Epigenetic reprogramming by adenovirus e1a. Science. 2008;321:1086-8. PMC2693122
Nazarian R, Shi H, Wang Q, Kong X, Koya RC, Lee H, Chen Z, Lee MK, Attar N, Sazegar H, Chodon T, Nelson SF, McArthur G, Sosman JA, Ribas A, Lo RS. Melanomas acquire resistance to B-RAF(V600E) inhibition by RTK or N-RAS upregulation. Nature. 2010;468:973-7. PMC3143360
Poulikakos PI, Persaud Y, Janakiraman M, Kong X, Ng C, Moriceau G, Shi H, Atefi M, Titz B, Gabay MT, Salton M, Dahlman KB, Tadi M, Wargo JA, Flaherty KT,
Kelley MC, Misteli T, Chapman PB, Sosman JA, Graeber TG, Ribas A, Lo RS, Rosen N, Solit DB. RAF inhibitor resistance is mediated by dimerization of aberrantly spliced BRAF(V600E). Nature. 2011;480:387-90. PMC3266695
Sridharan R, Tchieu J, Mason MJ, Yachechko R, Kuoy E, Horvath S, Zhou Q, Plath K. Role of the murine reprogramming factors in the induction of pluripotency. Cell. 2009;136:364-77. PMC3273494
Wu X, Ye Y, Kiemeney LA, Sulem P, Rafnar T, Matullo G, Seminara D, Yoshida T, Saeki N, Andrew AS, Dinney CP, Czerniak B, Zhang ZF, Kiltie AE, Bishop DT,
Vineis P, Porru S, Buntinx F, Kellen E, Zeegers MP, Kumar R, Rudnai P, Gurzau E, Koppova K, Mayordomo JI, Sanchez M, Saez B, Lindblom A, de Verdier P, Steineck
G, Mills GB, Schned A, Guarrera S, Polidoro S, Chang SC, Lin J, Chang DW, Hale KS, Majewski T, Grossman HB, Thorlacius S, Thorsteinsdottir U, Aben KK, Witjes
JA, Stefansson K, Amos CI, Karagas MR, Gu J. Genetic variation in the prostate stem cell antigen gene PSCA confers susceptibility to urinary bladder cancer. Nat Genet.
Shi H, Moriceau G, Kong X, Lee MK, Lee H, Koya RC, Ng C, Chodon T, Scolyer RA, Dahlman KB, Sosman JA, Kefford RF, Long GV, Nelson SF, Ribas A, Lo RS.
Melanoma whole-exome sequencing identifies (V600E)B-RAF amplificationmediated acquired B-RAF inhibitor resistance. Nat Commun. 2012;3:724.
Tanaka H, Kono E, Tran CP, Miyazaki H, Yamashiro J, Shimomura T, Fazli L, Wada R, Huang J, Vessella RL, An J, Horvath S, Gleave M, Rettig MB, Wainberg ZA, Reiter RE. Monoclonal antibody targeting of N-cadherin inhibits prostate cancer growth, metastasis and castration resistance. Nat Med. 2010;16:1414-20. PMC3088104
Tchieu J, Kuoy E, Chin MH, Trinh H, Patterson M, Sherman SP, Aimiuwu O, Lindgren A, Hakimian S, Zack JA, Clark AT, Pyle AD, Lowry WE, Plath K. Female human iPSCs retain an inactive X chromosome. Cell Stem Cell. 2010;7:329-42. PMC2935700« Show less
The UCLA Immunogenetics Center (UIC) provides comprehensive testing for organ and tissue transplantation. Transplant testing has a long history at UCLA. HLA typing was pioneered here in the 1960's. The development of the microcytoxicity test in 1964 marked the beginning of... Show more »
The UCLA Immunogenetics Center (UIC) provides comprehensive testing for organ and tissue transplantation. Transplant testing has a long history at UCLA. HLA typing was pioneered here in the 1960's. The development of the microcytoxicity test in 1964 marked the beginning of international testing and standardization of HLA typing. The UCLA Immunogenetics Center has retained its leadership position in HLA research, and in the development of accompanying diagnostic testing. In the intervening years, laboratory and scientific personnel, from the US and abroad, have learned HLA typing through courses and workshops at the UCLA Immunogenetics laboratory. The center provides immunogenetics and histocompatibility testing services to a wide range of physicians, patients, pharmaceutical companies and other medical facilities. The laboratory is a World Health Organization reference laboratory for HLA, and is licensed by the State of California, New York, Maryland, Pennsylvania and Rhode Island, is CMS certified and accredited by the American Society for Histocompatibility and Immunogenetics.« Show less
The Translational Pathology Core Laboratory (TPCL) is a research facility in the UCLA Department of Pathology and Laboratory Medicine and a UCLA Jonsson Comprehensive Cancer Center Shared Facility. Since 1996, the TPCL has provided an array of pathology-related services in support of... Show more »
The Translational Pathology Core Laboratory (TPCL) is a research facility in the UCLA Department of Pathology and Laboratory Medicine and a UCLA Jonsson Comprehensive Cancer Center Shared Facility. Since 1996, the TPCL has provided an array of pathology-related services in support of basic, translational and clinical research at UCLA. Our mission is to provide the highest quality pathology services in an efficient and cost-effective manner. The TPCL also provides expert consultative services to investigators in pathology-related study design, tissue selection, microscopic interpretation, immunohistochemistry, laser capture microdissection, digital image analysis, and IRB-related tissue questions.« Show less
GenoSeq, the UCLA Genotyping and Sequencing Core, is a state-of-the-art laboratory facility equipped with the latest genomic technologies. The GenoSeq Core provides services to research groups on the UCLA campus and in the broader scientific community. The Core contributes to research... Show more »
GenoSeq, the UCLA Genotyping and Sequencing Core, is a state-of-the-art laboratory facility equipped with the latest genomic technologies. The GenoSeq Core provides services to research groups on the UCLA campus and in the broader scientific community. The Core contributes to research throughout the world, including projects from Europe, Asia, Australia, South America, and a project analyzing samples from Antarctica. Approximately 4000 scientists from over 600 academic, government, and commercial labs have used the Core since 2000. The Core is part of the UCLA David Geffen School of Medicine, whose Medical Center has been rated one of the country’s top three hospitals, and number one on the west coast, for 18 consecutive years.
The GenoSeq Core is involved in a broad range of scientific research. Medical research performed at the GenoSeq Core includes studies of human neurological disorders, such as autism, bipolar disorder, and epilepsy; cancers of breast, colon, lung, and prostate; cardiac disease; and digestive disorders. The GenoSeq Core also carries out studies of the genetics of non-human organisms including wolves in Yellowstone Park, endangered California condors, giraffes, whales, crickets, and oak.
The GenoSeq Core is closely linked to the UCLA Bioinformatics Core in Gonda Center, which provides the computing power and sophisticated software needed to analyze and interpret the unprecedented volume of genetic data being generated with modern laboratory automation.« Show less
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