1314031136000

University of Arizona Genetics Core

155 Orders Completed
Tucson, Arizona, US

About University of Arizona Genetics Core

Divison of: University of Arizona

The GLP University of Arizona Genetics Core offers access to state-of-the-art resources and services to help investigators, educators, students and the biotech community conduct and promote research in the field of genomics.

UAGC provides a wide range of molecular biology services and support. Our... Show more »

The GLP University of Arizona Genetics Core offers access to state-of-the-art resources and services to help investigators, educators, students and the biotech community conduct and promote research in the field of genomics.

UAGC provides a wide range of molecular biology services and support. Our fee-per-use services inculding: DNA Extraction, Transgenic Mouse Genotyping, Human Cell Line Authentication, Sanger DNA Sequencing, Next-Generation Sequencing, Microsatellite DNA Typing, Sequenom Multiplex SNP Genotyping and Methylation Analysis, Real-time PCR and many others are available at a low cost to the entire research community.

Certifications:

  • GLP
  • CLIA/CAP

Instrumentation:

  • ABI PRISM 3730 genetic analyzer
  • Promega PowerPlex 16HS assay
  • Biotek Synergy microplate reader
  • Nanodrop
  • MassARRAY MALDI-TOF MS
  • ABI7900
    -Illumina HiSeq2500
    -Illumina MiSeq
    -Illumina Next Seq500
    -Nanostring NCounter
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Our Services (27)


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DNA/RNA Analysis and Quality Control

Starting at $66.00 per chip

DNA or RNA QC using the Agilent 2100 Bioanalyzer

DNA or RNA QC using the Agilent 2100 Bioanalyzer

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Agilent Bioanalyzer

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DNA Fragment Analysis

Starting at $1.87 per sample

Fragment analysis with 4 or 5 dye chemistry. Standard ladder is GS500, GS1200LIZ available upon request

Fragment analysis with 4 or 5 dye chemistry. Standard ladder is GS500, GS1200LIZ available upon request

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Illumina NGS Library Preparation

Starting at $200.00 per library
Request a quote for more information about this service.

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Illumina Sequencing

Starting at $1,300.20 per chip

UAGC offers Illumina HiSeq 2500, MiSeq and NextSeq500 for NGS sequencing. Library preparation charges are in addition to sequencing rate- please inquire. Various read lengths and sequencing outputs available

UAGC offers Illumina HiSeq 2500, MiSeq and NextSeq500 for NGS sequencing. Library preparation charges are in addition to sequencing rate- please inquire. Various read lengths and sequencing outputs available

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Illumina HiSeq 2500 Illumina MiSeq Illumina NextSeq 500

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NanoString nCounter Analysis

Starting at $630.00 per run

The Nanostring nCounter system can power you gene expression experiments. Molecular "barcodes" allows for multiplexing of multiple gene targets per run. Each run is 1 cartridge- can accommodate up to 12 samples

The Nanostring nCounter system can power you gene expression experiments. Molecular "barcodes" allows for multiplexing of multiple gene targets per run. Each run is 1 cartridge- can accommodate up to 12 samples

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Qiagen BioSprint

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Cell Line Authentication

Starting at $66.00 per sample

This service is ideal for researchers who wish to perform identity verification of human cell lines. We perform autosomal STR typing, using the Promega PowerPlex16HS assay. Sample scores (allelic values), comments about the sample profile match to known cell lines, and electropherogram trace data will be communicated to client... Show more »

This service is ideal for researchers who wish to perform identity verification of human cell lines. We perform autosomal STR typing, using the Promega PowerPlex16HS assay. Sample scores (allelic values), comments about the sample profile match to known cell lines, and electropherogram trace data will be communicated to client for any further applications and analysis.

*Please note: there are no separate tiers of service, everything, including a signed report, is included in this price

This service includes:
DNA Dilution
PCR with the PowerPlex16HS Kit from Promega
Fragment Analysis-AB 3730 Genetic Analyzer
First pass of data
Second data check
Search of sample profile in published cell line databases
Transmission of scores and/or trace data

This price if for receipt of gDNA. DNA extraction from a cell pellet starts at $9.60/sample.

Turnaround time: 1 week

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Applied Biosystems 3730xl DNA Sequencer Qiagen BioSprint Human

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Capillary Electrophoresis

Starting at $1.87 per sample

UAGC offers Fragment Analysis services with our two ABI3730 DNA Analyzers. The 3730 employs an array of 48 capillaries to electrophoretically separate fluorescently labeled DNA fragments.

Multiplexed PCR or pooled reactions amplified with compatible labeled primers are scored with the ABI PRISM® 3730 DNA Analyzer.

Fragment... Show more »

UAGC offers Fragment Analysis services with our two ABI3730 DNA Analyzers. The 3730 employs an array of 48 capillaries to electrophoretically separate fluorescently labeled DNA fragments.

Multiplexed PCR or pooled reactions amplified with compatible labeled primers are scored with the ABI PRISM® 3730 DNA Analyzer.

Fragment Analysis Applications:
Microsatellite Analysis
Genotyping
Mutation Detection

This service includes:
PCR product dilution and formamide preparation
Internal Size Standard- GS 500 Liz or ROX
Post Processing and QC step

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Qiagen BioSprint

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Genomic DNA Extraction

Starting at $12.00 per sample

UAGC offers high-throughput, high-recovery, quick and easy, Oragene Discover Kit DNA extraction services as well as RNA extraction*

HIGH-THROUGHPUT DNA EXTRACTION SERVICE:
Automated DNA extraction service for sample sizes 48+
Capacity for up to 1,000 samples per day
$12.00 per sample (Academic discounts may apply)

HIGH... Show more »

UAGC offers high-throughput, high-recovery, quick and easy, Oragene Discover Kit DNA extraction services as well as RNA extraction*

HIGH-THROUGHPUT DNA EXTRACTION SERVICE:
Automated DNA extraction service for sample sizes 48+
Capacity for up to 1,000 samples per day
$12.00 per sample (Academic discounts may apply)

HIGH RECOVERY DNA EXTRACTION:
For samples of limited source material, high inhibition or for high molecular weight and high quantity DNA recovery.

ORAGENE DNA EXTRACTION:
UAGC is a certified member of the DNA Genotek (maker of the Oragene DNA Collection Kit) Partner Program.

Quick and Easy DNA EXTRACTION:
UAGC offers this simple extraction as a low-cost option. This is ideal for certain projects and sample types (i.e. screening or genotyping of mouse tails or fruit flies). This extraction protocol does not involve any purification of DNA.

Fees:
High Throughput DNA Extraction (per sample)
External Academic/Non-Profit $9.60
Industry $12.00

High Recovery DNA Extraction (per sample)
External Academic/Non-Profit $18.00
Industry $22.50

Quick and Easy DNA Extraction (per sample)
External Academic/Non-Profit $2.40
Industry $3.00

Oragene•Discover Kit DNA Extraction (per plate)
External Academic/Non-Profit $1,067.34
Industry 96-well

*Please contact lab for RNA extraction pricing, or extraction from material classified as BSL2

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Qiagen BioSprint

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RNA Extraction and Purification

Starting at $33.60 per sample

RNA isolation from any tissue type is available from the UAGC. Ask us about these features add-ons
Tubes of RNAlater
Tissue homogenization
RNA QC

RNA isolation from any tissue type is available from the UAGC. Ask us about these features add-ons
Tubes of RNAlater
Tissue homogenization
RNA QC

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SNP Genotyping

Single Nucleotide Polymorphism Genotyping
Starting at $4.20 per reaction

Taqman Genotype: cost per SNP- does not include assay reagents

Agena Mass Array SNP genotyping also available:
UAGC offers SNP genotyping on the Sequenom MassARRAY® MALDI–TOF instrument. Using this technology we can multiplex SNPs for up to 36 loci per reaction. This is the most cost effective method to genotype many SNPs... Show more »

Taqman Genotype: cost per SNP- does not include assay reagents

Agena Mass Array SNP genotyping also available:
UAGC offers SNP genotyping on the Sequenom MassARRAY® MALDI–TOF instrument. Using this technology we can multiplex SNPs for up to 36 loci per reaction. This is the most cost effective method to genotype many SNPs in many samples.

SNP Genotyping is available in 96-well and 384 well plates with approx 30 SNP's per multiplex. Contact us for experimental design details and experiment specific pricing information.

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MALDI-TOF-MS Qiagen BioSprint

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Sanger Sequencing

Starting at $210.00 per plate

UACG can sequence your PCR product using our 2 AB 3730 instruments. Turn around time is 3-4 days. We offer this service for tubes or 96-well plates. (Discount for 96-well plate sequencing)

Price for sequencing:
Low Volume (tubes) DNA Sequencing: $8.00 - per sample/ 1 direction.
High Volume (96 well plate) DNA Sequencing:... Show more »

UACG can sequence your PCR product using our 2 AB 3730 instruments. Turn around time is 3-4 days. We offer this service for tubes or 96-well plates. (Discount for 96-well plate sequencing)

Price for sequencing:
Low Volume (tubes) DNA Sequencing: $8.00 - per sample/ 1 direction.
High Volume (96 well plate) DNA Sequencing: $210.00per 96 well plate- plate cleanup, quantification and normalization also available.

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Applied Biosystems 3730xl DNA Sequencer

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Transgenic Genotyping

Starting at $5.40 per reaction

We can genotype your transgenic mice. Multiple tests from 1 tail tip. Quick and Easy DNA extraction available for $2.40. We can provide bar-coded tubes, you add the tail tips and we will take care of the rest. Contact us to ask about our prevalidated assays.

Sample Input
We provide barcoded collection tubes pre-filled with... Show more »

We can genotype your transgenic mice. Multiple tests from 1 tail tip. Quick and Easy DNA extraction available for $2.40. We can provide bar-coded tubes, you add the tail tips and we will take care of the rest. Contact us to ask about our prevalidated assays.

Sample Input
We provide barcoded collection tubes pre-filled with extraction buffer. Simply add 2-4mm of tail tissue and return to the UAGC. Samples with STE can be shipped at ambient temperature. Other tissues can be utilized, please email for details.

Expected Results
Depending on the locus, the UAGC utilizes a variety of methods to generate data on your samples, including fragment analysis, Taqman scatter plot, and gel electrophoresis.

Turnaround time: 5 business days

The UAGC provides Transgenic Mouse Genotyping Services. Select your region of interest (to see full list; see MouseAssay_list attachment in sidebar), or we can design a custom assay to genotype your specific line.

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ABI 3730XL

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Whole Exome Sequencing (WES)

Price on request

The HiSeq 2500 with 2x100 PE chemistry can produce 25-30GB data per lane. Library preparation services are available.

The Illumina HiSeq 2500 may be used for:
Whole genome sequencing (de novo, resequencing) via:
• Shotgun sequencing (bacteria, viruses) and
• BAC-based shotgun sequencing (animals, plants).
Amplicon... Show more »

The HiSeq 2500 with 2x100 PE chemistry can produce 25-30GB data per lane. Library preparation services are available.

The Illumina HiSeq 2500 may be used for:
Whole genome sequencing (de novo, resequencing) via:
• Shotgun sequencing (bacteria, viruses) and
• BAC-based shotgun sequencing (animals, plants).
Amplicon sequencing
• RAD Seq
Small RNA sequencing, including expressed sequence tags (ESTs)
• Assembled de novo for novel organisms or
• Mapped to genome or transcriptome references for characterized organisms
Transcriptome analysis

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Illumina HiSeq 2500 Qiagen BioSprint

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qPCR

Quantitative PCR
Starting at $5.00 per sample

Real Time PCR provides a quantitative assessment of your sample for gene expression, gene transcription studies or targeted nucleic acids.

Equipment:
ABI7300
ABI7900

Additional QC steps, sample prep protocols and assay charges may apply. Please inquire for a more detailed quote.

Real Time PCR provides a quantitative assessment of your sample for gene expression, gene transcription studies or targeted nucleic acids.

Equipment:
ABI7300
ABI7900

Additional QC steps, sample prep protocols and assay charges may apply. Please inquire for a more detailed quote.

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ABI 7900HT ABI 7300

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Genotyping and Gene Expression Assays

Price on request
Request a quote for more information about this service.

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RT-PCR

Reverse transcription polymerase chain reaction
Price on request
Request a quote for more information about this service.

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DNA Sequencing

Price on request
Request a quote for more information about this service.

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DNA Extraction and Purification

Price on request
Request a quote for more information about this service.

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Whole Exome Sequence Capture

Price on request
Request a quote for more information about this service.

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Gel Staining

Price on request
Request a quote for more information about this service.

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Gel Imaging

Price on request
Request a quote for more information about this service.

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Sequenom Targeted SNP Genotyping

Sequenom targeted single nucleotide polymorphism genotyping
Price on request
Request a quote for more information about this service.

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TaqMan Assays

TaqMan Gene Expression Assays
Price on request
Request a quote for more information about this service.

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DNA Quantification

Price on request
Request a quote for more information about this service.

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Shotgun DNA Sequencing

Price on request
Request a quote for more information about this service.

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Plasmid Sequencing

Price on request
Request a quote for more information about this service.

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Amplicon Sequencing

Price on request
Request a quote for more information about this service.

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Jose Carranza

Research Technician, NGS
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Elizabeth Cox

Research Specialist, DNA Extraction, Sample QC, Cell Line Authentication, Genotyping
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Barbara Fransway

Manager, Genomic Research Services
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Jonathan Galina-Mehlman

Research Specialist, NGS team
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Heather Issar

Research Specialist, MassArray Genotyping
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Crystal Richt

Research Specialist, Nanostring
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James Shira

Research Specialist, RNA, TaqMan Genotyping, Real Time PCR

FileType/PDF Created with Sketch. Current_UAGC_assay_list_Oct2014.pdf

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  • Positive review for RT-PCR:

    August 2018

    "Great service, definitely reccomend"

  • Positive review for Cell Line Authentication:

    February 2018

    "The service was timely and good."

  • Positive review for Cell Line Authentication:

    December 2017

    "Fast turnaround and reasonably priced. Will definitely use again!"

  • Positive review for Cell Line Authentication:

    September 2017

    "Great service, fast return of results."

  • Positive review for Cell Line Authentication:

    July 2017

    "Excellent service! Quick and easy, received prompt responses for all my questions. Highly recommend."

  • Positive review for Cell Line Authentication:

    July 2017

    "They did our cell authentication. Every time I have questions, they answered immediately, and they were very nice. Result came back promptly. Excellent core."

  • Positive review for Cell Line Authentication:

    February 2017

    "The service was very quick and painless with great results."

  • Positive review for Cell Line Authentication:

    December 2016

    "Dear Science Exchange, Thank you so much for help us do the cell line authentication. Elizabeth is great, she is very nice to work with. Only this time we didn't figure out what cause the contamination. we will keep contact to figure out what cause the contamination, cell differentiation or contaminated by other cell line? Best Regards, Zhaohong Yin"

  • Positive review for Cell Line Authentication:

    December 2016

    "Barbara and her team have done a great job in authentication of my cell lines. They provided prompt and professional service."

  • Positive review for Transgenic Genotyping:

    December 2016

    "I'm very pleased with the genotyping service that Liz Cox at the University of Arizona is providing for me. She's currently working on four separate genotyping assays for me and provides data in a timely manner. I'm very happy with the service she provides, as it frees up time for my staff to work on other projects. I will certainly use the genotyping service again in the very near future."

  • Positive review for Cell Line Authentication:

    October 2016

    "Fast service. Good quality results."

  • Positive review for Cell Line Authentication:

    July 2016

    "Excellent and efficient service!"

  • Positive review for Cell Line Authentication:

    July 2016

    "The service was so good."

  • Positive review for Cell Line Authentication:

    May 2016

    "Timely processing of samples and great accuracy. Thank you so much for the service you guys provide!"

  • Positive review for Cell Line Authentication:

    April 2016

    "The lab was very responsive to my inquiries and the service was delivered in a timely manner. I highly recommend this service."

  • Positive review for Cell Line Authentication:

    April 2016

    "The service is very professional and thorough. So thankful that Genetics Core/Powerplex is on campus and available for everyone. Great service and would like to utilize it in future. Thank you!"

  • Positive review for Cell Line Authentication:

    April 2016

    "I am very satisfied with the work performed by University of Arizona Genetics Core."

  • Positive review for Cell Line Authentication:

    March 2016

    "High quality service. Fast. Excellent customer service and assistance. I will recommend it."

  • Positive review for Cell Line Authentication:

    March 2016

    "I'm really very happy working with University of Arizona Genetics Core and intend to work again with in future. The service regarding the quality of data and timeline I received from them is very satisfactory. I will refer University of Arizona Genetics Core facility to my freinds/colleagues."

  • Positive review for Cell Line Authentication:

    March 2016

    "I will definitely work with University of Arizona again and again. And I will recommend it highly to my colleagues and collaborators. I am very satisfied with the process of the work. The timing, the accuracy, the immediate responses to my questions and the pricing. Excellent work. Thanks."

  • Positive review for Cell Line Authentication:

    March 2016

    "I'm very happy with the service. Data quality, communication, and time frame of completion are superb! I'll definitely use your service in future requirements. Thanks, Jharna"

  • Positive review for Cell Line Authentication:

    February 2016

    "High quality service. Fast. Excellent customer service and assistance. I will definitely recommend and use again anytime I need"

  • Positive review for qPCR:

    October 2015

    "we are very satisfied with services of UAGC."

  • Positive review for Cell Line Authentication:

    July 2015

    "The cell line authentication service was timely and provided clear and easy-to-interpret results. Customer service was excellent."

  • Positive review for Cell Line Authentication:

    July 2015

    "Very happy with the service provided by UAGC. Great communication and customer service and support, and very reasonable turnaround times. Wouldn't hesitate to recommend this lab for cell line authentication services or work with them again on any future projects."

  • Positive review for RNA Extraction and Purification:

    June 2015

    "The team at University of Arizona Genetics Core, including Colleen Ramsower and Barbara Fransway, is excellent. The turn around on RNA and DNA extractions is quick, samples are high quality and are labelled and packaged carefully in a very thoughtful manner. I appreciate the great attention to detail. Utilization of services at this core has helped my projects progress more efficiently."

  • Positive review for Cell Line Authentication:

    March 2015

    "efficient and a pleasant service experience"

  • Positive review for Cell Line Authentication:

    September 2014

    "Professional service. Very reliable and efficient."

  • Positive review for Cell Line Authentication:

    May 2012

    "Great turn around time. Easy to interpret results. Always available for questions. Great rates for cell authentication project!"

University of Arizona Genetics Core has not received any endorsements.