1314031136000

University of Arizona Genetics Core

Tucson, Arizona

The GLP University of Arizona Genetics Core offers access to state-of-the-art resources and services to help investigators, educators, students and the biotech community conduct and promote research in the field of genomics.

UAGC provides a wide range of molecular biology services and support. Our fee-per-use services inculding: DNA Extraction, Sample Banking, Transgenic Mouse Genotyping, Human Cell Line Authentication, Sanger DNA Sequencing, Next-Generation Sequencing, Microsatellite DNA Typing, Sequenom Multiplex SNP Genotyping and Methylation Analysis, Real-time PCR and many others are available at a low cost to the entire research community.

Certifications:
- GLP
- CLIA/CAP in progress

Instrumentation:
- ABI PROSM 3730 genetic analyzer
- Promega PowerPlex 16HS assay
- Biotek Synergy H1 microplater reader
- Nanodrop
- Ion Torrent PGM
- MassARRAY... Show more »

The GLP University of Arizona Genetics Core offers access to state-of-the-art resources and services to help investigators, educators, students and the biotech community conduct and promote research in the field of genomics.

UAGC provides a wide range of molecular biology services and support. Our fee-per-use services inculding: DNA Extraction, Sample Banking, Transgenic Mouse Genotyping, Human Cell Line Authentication, Sanger DNA Sequencing, Next-Generation Sequencing, Microsatellite DNA Typing, Sequenom Multiplex SNP Genotyping and Methylation Analysis, Real-time PCR and many others are available at a low cost to the entire research community.

Certifications:
- GLP
- CLIA/CAP in progress

Instrumentation:
- ABI PROSM 3730 genetic analyzer
- Promega PowerPlex 16HS assay
- Biotek Synergy H1 microplater reader
- Nanodrop
- Ion Torrent PGM
- MassARRAY MALDI-TOF MS
- iCycler iQ
- ABI7900
- Illumina HiSeq2000
- Roche 454

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    Capillary electrophoresis
    $1.20 USD
    per Sample

    UAGC offers Fragment Analysis services with our two ABI3730 DNA Analyzers. The 3730 employs an array of 48 capillaries to electrophoretically separate fluorescently labeled DNA fragments.

    Multiplexed PCR or pooled reactions amplified with compatible labeled primers are scored with the ABI PRISM® 3730 DNA... Show more »

    UAGC offers Fragment Analysis services with our two ABI3730 DNA Analyzers. The 3730 employs an array of 48 capillaries to electrophoretically separate fluorescently labeled DNA fragments.

    Multiplexed PCR or pooled reactions amplified with compatible labeled primers are scored with the ABI PRISM® 3730 DNA Analyzer.

    Fragment Analysis Applications:
    Microsatellite Analysis
    Genotyping
    Mutation Detection

    This service includes:
    PCR product dilution and formamide preparation
    Internal Size Standard- GS 500 Liz or ROX
    Post Processing and QC step

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    Cell line authentication
    $60.00 USD
    per Sample

    This service is ideal for researchers who wish to perform identity verification of human cell lines. We perform autosomal STR typing, using the Promega PowerPlex16HS assay. The inquiring party is responsible for the location and use of a reference data set in order to confirm identity. Profiles are suitable for verification using... Show more »

    This service is ideal for researchers who wish to perform identity verification of human cell lines. We perform autosomal STR typing, using the Promega PowerPlex16HS assay. The inquiring party is responsible for the location and use of a reference data set in order to confirm identity. Profiles are suitable for verification using supplier databases including ATCC,DSMZ,JCRB and others. Sample scores (allelic values), and electropherogram trace data will be communicated to client for any further applications and analysis.

    This service includes:
    DNA Dilution
    PCR with the PowerPlex16HS Kit from Promega
    Agarose gel to confirm amplification
    Fragment Analysis-AB 3730 Genetic Analyzer
    First pass of data
    Second data check
    Transmission of scores and/or trace data

    Turnaround time: 1 week

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    Genomic DNA extraction
    $12.00 USD
    per Sample

    UAGC offers high-throughput, high recovery, quick and easy, Oragene Discover Kit, DNA extraction services as well as RNA extraction.

    HIGH-THROUGHPUT DNA EXTRACTION SERVICE:
    Automated DNA extraction service for sample sizes 48+
    Capacity for up to 1,000 samples per day
    $10.89 per sample (Academic discounts may... Show more »

    UAGC offers high-throughput, high recovery, quick and easy, Oragene Discover Kit, DNA extraction services as well as RNA extraction.

    HIGH-THROUGHPUT DNA EXTRACTION SERVICE:
    Automated DNA extraction service for sample sizes 48+
    Capacity for up to 1,000 samples per day
    $10.89 per sample (Academic discounts may apply)

    HIGH RECOVERY DNA EXTRACTION:
    For samples of limited source material, high inhibition or for high molecular weight and high quantity DNA recovery.

    ORAGENE DNA EXTRACTION:
    UAGC is a certified member of the DNA Genotek (maker of the Oragene DNA Collection Kit) Partner Program.

    Quick and Easy DNA EXTRACTION:
    UAGC offers this simple extraction as a low-cost option. This is ideal for certain projects and sample types (i.e. screening or genotyping of mouse tails or fruit flies).

    Fees:

    - High Throughput DNA Extraction (per sample)
    Academic $4.19
    Non-Academic $6.00
    Industry $7.00

    - High Recovery DNA Extraction (per sample)
    Academic $10.00
    Non-Academic $12.00
    Industry $16.00

    - Quick and Easy DNA Extraction (per sample)
    Academic $1.38
    Non-Academic $2.00
    Industry $3.00

    - Oragene•Discover Kit DNA Extraction (per plate)
    Academic $1,067.34
    Non-Academic $1,067.34
    Industry 96-well

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    Ion Torrent NGS
    Ion Torrent next generation sequencing
    $715.95 USD
    per Sample

    UAGC accepts Genomic DNA, PCR product, BACs, cDNA, and RNA for sequencing on the Ion Torrent Personal Genome Machine.

    Applications include:
    Small Genome Sequencing-Microbial and viral de novo & resequencing
    Targeted Resequencing (Amplicon)
    Whole Genome/Exome Validation-validate other platform... Show more »

    UAGC accepts Genomic DNA, PCR product, BACs, cDNA, and RNA for sequencing on the Ion Torrent Personal Genome Machine.

    Applications include:
    Small Genome Sequencing-Microbial and viral de novo & resequencing
    Targeted Resequencing (Amplicon)
    Whole Genome/Exome Validation-validate other platform results
    RNA Sequencing-Whole Transcriptome

    Ion Torrent Pricing is shown for For-Profit Customers. Non profit/ academic users please contact us for rate information.

    Ion Torrent Library Construction-DNA: $782.55/sample
    Whole Transcriptome: $715.95/sample
    Ion Torrent Template Preparation- $499.50/sample

    314 Chip Sequencing:
    One chip per initialization: $932.40/chip
    Two chips per initialization: $799.20/chip

    316 Chip Sequencing:
    One chip per initialization: Please inquire
    Two chips per initialization: Please inquire

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    Methylation analysis
    $11.70 USD
    per Sample

    UACG offers Methylation Analysis using the Sequenom MassARRAY MALDI-TOF Instrument. This technique can provide methylation ratios for CpGs within a target sequence from in the range of 10-90%. This process can be scaled to accommodation many regions for multiple samples. Cost is $4508.00 - per 384 well plate ($11.74 per sample). Does not include primer cost.

    UACG offers Methylation Analysis using the Sequenom MassARRAY MALDI-TOF Instrument. This technique can provide methylation ratios for CpGs within a target sequence from in the range of 10-90%. This process can be scaled to accommodation many regions for multiple samples. Cost is $4508.00 - per 384 well plate ($11.74 per sample). Does not include primer cost.

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    RNA extraction
    $16.00 USD
    per Sample

    RNA isolation from any tissue type is available from the UAGC. This service includes:
    Tubes of RNAlater
    Tissue homogenization
    RNA extraction
    RNA Isolation

    Fees:

    RNA Extraction (per sample)
    Academic $8.00
    Non-Academic $9.00
    Industry $10.00

    RNA isolation from any tissue type is available from the UAGC. This service includes:
    Tubes of RNAlater
    Tissue homogenization
    RNA extraction
    RNA Isolation

    Fees:

    RNA Extraction (per sample)
    Academic $8.00
    Non-Academic $9.00
    Industry $10.00

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    Roche 454 NGS
    Roche 454 next generation sequencing
    $6,216.00 USD
    per Plate

    UAGC accepts Genomic DNA, PCR products, BACs, cDNA for genome sequencing on the Roch 454 FLX instrument.

    Applications include:
    Whole genome sequencing (de novo, resequencing) via Shotgun sequencing (bacteria, viruses) and BAC-based shotgun sequencing (animals, plants)
    Amplicon sequencing
    Small RNA... Show more »

    UAGC accepts Genomic DNA, PCR products, BACs, cDNA for genome sequencing on the Roch 454 FLX instrument.

    Applications include:
    Whole genome sequencing (de novo, resequencing) via Shotgun sequencing (bacteria, viruses) and BAC-based shotgun sequencing (animals, plants)
    Amplicon sequencing
    Small RNA sequencing, including expressed sequence tags (ESTs)
    Transcriptome analysis

    Expected results include sequence data assembled de novo for novel organisms or mapped to genome or transcriptome references for characterized organisms, 3kb, 8kb and 20kb Paired End Library Preparation and Sequencing.

    Pricing:
    Sample Preparation- $333 per sample (NOTE- this charge does not apply for most samples. Sample prep charge only applies if we are synthesizing cDNA from RNA or extracting genetic material for you.
    Library Preparation (General or Rapid) - $666 per sample
    Titrations - $333 per sample
    Bulk emPCR - $2,919 flat rate per whole plate
    Sequencing - $6,216 flat rate per whole plate

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    SNP genotyping
    Single Nucleotide Polymorphism genotyping
    Price on request

    Taqman Genotype: $1.05 per SNP

    Taqman Genotype: $1.05 per SNP

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    Sanger Sequencing
    $8.00 USD
    per Sample

    UACG can sequence your PCR product using our 2 AB 3730 instruments. Turn around time is 3-4 days. We offer this service for tubes or 96-well plates. (Discount for 96-well plate sequencing)

    Price for sequencing:
    Low Volume (tubes) DNA Sequencing: $8.00 - per sample/ 1 direction.
    High Volume (96 well plate) DNA Sequencing: $167.00per 96 well plate

    UACG can sequence your PCR product using our 2 AB 3730 instruments. Turn around time is 3-4 days. We offer this service for tubes or 96-well plates. (Discount for 96-well plate sequencing)

    Price for sequencing:
    Low Volume (tubes) DNA Sequencing: $8.00 - per sample/ 1 direction.
    High Volume (96 well plate) DNA Sequencing: $167.00per 96 well plate

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    Sequenom
    Sequenom genotyping
    $4.56 USD
    per Sample

    SNP Genotyping: $1,750.00 - per 384 well plate/ per 30 SNP's = $4.56 per sample ($0.15 per SNP)

    DNA Methylation Analysis: $2,641.00 - per 384 well plate

    SNP Genotyping: $1,750.00 - per 384 well plate/ per 30 SNP's = $4.56 per sample ($0.15 per SNP)

    DNA Methylation Analysis: $2,641.00 - per 384 well plate

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    Transgenic genotyping
    $3.90 USD
    per Sample

    We can genotype your transgenic mice. Multiple tests from 1 tail tip. Quick and Easy DNA extraction included. We can provide bar-coded tubes, you add the tail tips and we will take care of the rest. Contact us to ask about our prevalidated assays.

    We can genotype your transgenic mice. Multiple tests from 1 tail tip. Quick and Easy DNA extraction included. We can provide bar-coded tubes, you add the tail tips and we will take care of the rest. Contact us to ask about our prevalidated assays.

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    Whole exome seq
    Whole exome sequencing
    Price on request

    The HiSeq 2000 delivers the industry's highest sequencing output with a Single Read generating up to 3 billion clusters and a Paired-End Read up to 6 billion.

    The Illumina HiSeq 2000 may be used for:
    Whole genome sequencing (de novo, resequencing) via:
    • Shotgun sequencing (bacteria, viruses) and Show more »

    The HiSeq 2000 delivers the industry's highest sequencing output with a Single Read generating up to 3 billion clusters and a Paired-End Read up to 6 billion.

    The Illumina HiSeq 2000 may be used for:
    Whole genome sequencing (de novo, resequencing) via:
    • Shotgun sequencing (bacteria, viruses) and
    • BAC-based shotgun sequencing (animals, plants).
    Amplicon sequencing
    • RAD Seq
    Small RNA sequencing, including expressed sequence tags (ESTs)
    • Assembled de novo for novel organisms or
    • Mapped to genome or transcriptome references for characterized organisms
    Transcriptome analysis

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    qPCR
    Quantitative PCR
    Price on request

    UAGC now offers assistance with PCR. We have a skilled team of technicians and a large bank of BioRad-MJ thermocyclers to support your DNA amplification needs. Real Time PCR provides a quantitative assessment of your sample for gene expression, gene transcription studies or targeted nucleic acids.

    Our PCR related services... Show more »

    UAGC now offers assistance with PCR. We have a skilled team of technicians and a large bank of BioRad-MJ thermocyclers to support your DNA amplification needs. Real Time PCR provides a quantitative assessment of your sample for gene expression, gene transcription studies or targeted nucleic acids.

    Our PCR related services include but are not limited to:
    Primer design
    Primer validation
    PCR optimization
    High throughput sample testing
    Reaction multiplexing

    Equipment:
    ABI7300
    iCycler IQ
    ABI7900

    Fees:
    Academic: $38.00/hour
    Industry: $58.00/hour

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Roshan Karki gave a positive review for Cell line authentication:

May 06, 2012
Great turn around time. Easy to interpret results. Always available for questions. Great rates for cell authentication project!

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