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UNC Macromolecular Interactions Facility

Chapel Hill, North Carolina, US

The Macromolecular Interactions Facility (UNC folks fondly call it the "Mac-In-Fac") is a core facility of the University of North Carolina at Chapel Hill. In operation since 1996, it provides instrumentation and resources for biophysical characterization of biological macromolecules and their interactions with cognate ligands. Core instrumentation includes surface plasmon resonance (SPR)-based and bio-layer interferometry (BLI)-based biosensors, isothermal titration calorimeters (ITCs) including the fully-automated Auto-ITC200, a circular dichroism (CD) spectropolarimeter, static and dynamic light scattering instruments, a fluorescence microplate reader, a nano-DSF (nano-differential scanning fluorimeter), an analytical ultracentrifuge, and a spectrofluorometer. Though the facility was established for use by UNC researchers, we welcome researchers from other academic institutions and biotech companies. The... Show more »

The Macromolecular Interactions Facility (UNC folks fondly call it the "Mac-In-Fac") is a core facility of the University of North Carolina at Chapel Hill. In operation since 1996, it provides instrumentation and resources for biophysical characterization of biological macromolecules and their interactions with cognate ligands. Core instrumentation includes surface plasmon resonance (SPR)-based and bio-layer interferometry (BLI)-based biosensors, isothermal titration calorimeters (ITCs) including the fully-automated Auto-ITC200, a circular dichroism (CD) spectropolarimeter, static and dynamic light scattering instruments, a fluorescence microplate reader, a nano-DSF (nano-differential scanning fluorimeter), an analytical ultracentrifuge, and a spectrofluorometer. Though the facility was established for use by UNC researchers, we welcome researchers from other academic institutions and biotech companies. The service is strictly on first-come-first-served basis

Equipment
- Malvern/MicroCal Auto-iTC200: Fully-Automated Isothermal Titration Calorimeter
- Bio-Rad Proteon XPR36: Surface plasmon resonance (SPR)-based biosensor
- ForteBio Octet Red384: Bio-layer Interferometry (BLI)-based biosensor
- SEC-MALS system: Size Exclusion Column with Wyatt multi-angle light scattering system
- Wyatt DynaPro Dynamic Light Scattering Plate Reader
- Applied Photophysics Chirascan Plus Circular Dichroism (CD) spectrometer
- Nanotemper Prometheus NT.48 nanoDSF
- Beckman XL-I: Analytical Ultracentrifuge
- Malvern/MicroCal VP-ITC: Isothermal Titration Calorimeter
- PHERAstar FS Microplate Reader
- Horiba Jobin Yvon Fluorolog-3 T-format Spectrofluorometer

Publications
To date, close to 400 papers containing data obtained at the Mac-In-Fac have been published.
Here are some recent ones:
- A multi-laboratory Comparision of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation.120 authors including A. Tripathy. PLOS ONE (in press), 2015.
- Discovery of a selective, substrate-competitive inhibitor of the lysine methyltransferase SETD8. Ma A, Yu W, Li F, Bleich RM, Herold JM, Butler KV, Norris JL, Korboukh V, Tripathy A, Janzen WP, Arrowsmith CH, Frye SV, Vedadi M, Brown PJ, Jin J. J Med Chem. 2014 Aug 14;57(15):6822-33.
- The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. Nat Commun. 2014;5:3078.
- Molecular Basis for pH-Dependent Mucosal Dehydration in Cystic Fibrosis Airways. Garland, A.L., Walton, W.G., Coakley, R.D., Tan, C.D., Gilmore, R.C., Hobbs, C.A., Tripathy, A., Clunes, L.A., Bencharit, S., Stutts, M.J., Betts, L., Redinbo, M.R., and Tarran, R. Proceedings of the National Academy of Sciences USA 2013 110 (40) 15973-15978.
- Identification of novel integrin binding partners for CIB1: structural and thermodynamic basis of CIB1 promiscuity. Thomas C Freeman, Justin L Black, Holly G Bray, Onur Dagliyan, Yi I. Wu, Ashutosh Tripathy, Nikolay V. Dokholyan, Tina M Leisner, and Leslie V. Parise. Biochemistry 2013 Sept. DOI: 10.1021/bi400678y.
- Plant tumour biocontrol agent employs a tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase. Chopra, S., Palencia, A., Virus, C., Tripathy, A., Temple, B.R., Velazquez-Campoy, A., Cusack, S., and Reader, J. S Nature Communications 2013 4: 1417.

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UNC Macromolecular Interactions Facility has not listed any services.

Isothermal Titration Calorimetry (ITC)
Price on request

Instrument: Microcal/Malvern Auto-ITC200
Information Obtained: Stoichiometry (N), Equilibrium binding constant (Kd), deltaG, deltaH, and deltaS

Instrument: Microcal/Malvern Auto-ITC200
Information Obtained: Stoichiometry (N), Equilibrium binding constant (Kd), deltaG, deltaH, and deltaS

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NanoDSF
Price on request

Instrument: Nanotemper Prometheus NT.48 nanoDSF
Information Obtained: Melting temperature (Tm) and sample stability

Instrument: Nanotemper Prometheus NT.48 nanoDSF
Information Obtained: Melting temperature (Tm) and sample stability

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Circular Dichroism
Price on request

Instrument: Applied Photophysics Chirascan-Plus CD spectrometer
Information Obtained: secondary structure, melting profile, and melting temperature (Tm)

Instrument: Applied Photophysics Chirascan-Plus CD spectrometer
Information Obtained: secondary structure, melting profile, and melting temperature (Tm)

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SEC-MALS
Size exclusion chromatography with multiangle light scattering detectors
Price on request

Instruments: Wyatt DAWN Heleos-II, Optilab T-rex Refractometer, Agilent FPLC, and Superdex 200 column
Information Obtained: Molecular weight, oligomeric states, hydrodynamic and RMS radius

Instruments: Wyatt DAWN Heleos-II, Optilab T-rex Refractometer, Agilent FPLC, and Superdex 200 column
Information Obtained: Molecular weight, oligomeric states, hydrodynamic and RMS radius

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Dynamic Light Scattering
Price on request

Instrument: Wyatt DynaPro Plate Reader
Information Obtained: Hydrodynamic radius, presence of aggregates, and sample purity

Instrument: Wyatt DynaPro Plate Reader
Information Obtained: Hydrodynamic radius, presence of aggregates, and sample purity

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Melting Point Determination
Price on request

Instrument: Nanotemper Prometheus NT.48 nanoDSF
Information Obtained: Melting temperature (Tm) and sample stability

Instrument: Nanotemper Prometheus NT.48 nanoDSF
Information Obtained: Melting temperature (Tm) and sample stability

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Imaging & Spectroscopy
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Spectroscopy
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Scattering Techniques
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Chemistry and Materials
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Analytical Chemistry Services
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Purification Services
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Chromatography
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Size Exclusion Chromatography (SEC)
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Engineering and Fabrication
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Physical Analysis Methods
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Calorimetry
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2018-05-10 19:31:28 +0200

Net Promoter Score of 8 received for SEC-MALS.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 8.
2017-12-20 23:39:27 +0100

Net Promoter Score of 10 received for SEC-MALS.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
June 28, 2017

Positive review received for Isothermal Titration Calorimetry (ITC):

"The ITC work is good, and Professor AshTripathy has a good patient to answer my questions. Only one concerns for me is that if we do a lot of binding assays such as 100 reactions it will need over $30,000 funding, it will be too expansive to afford. Best! Fengzhi Li"

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