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UNC Eshelman School of Pharmacy - NMR Facility

Chapel Hill, North Carolina, US

The Eshelman School of Pharmacy NMR Facility at the University of North Carolina at Chapel Hill has had a diverse group of users with a broad range of interests. These include natural products or other "small" molecules; polymers; and biomolecular structure and dynamics. The Facility has a number of spectrometers, including an Inova 400, which is typically used for routine 1H or 13C one-dimensional experiments, and for those two-dimensional experiments usually associated with "small" molecules; e.g., COSY, ROESY, HSQC, HMBC, and so on. We also have a fully automated 400MR, which is also used for these experiments, as well as for 19F and 31P. In addition, the Laboratory has an Inova 500 which is now often used to study small molecules. However, it is designed for biomolecular NMR, and is therefore our most versatile instrument, with four channels instead of two; waveform generators on all four... Show more »

The Eshelman School of Pharmacy NMR Facility at the University of North Carolina at Chapel Hill has had a diverse group of users with a broad range of interests. These include natural products or other "small" molecules; polymers; and biomolecular structure and dynamics. The Facility has a number of spectrometers, including an Inova 400, which is typically used for routine 1H or 13C one-dimensional experiments, and for those two-dimensional experiments usually associated with "small" molecules; e.g., COSY, ROESY, HSQC, HMBC, and so on. We also have a fully automated 400MR, which is also used for these experiments, as well as for 19F and 31P. In addition, the Laboratory has an Inova 500 which is now often used to study small molecules. However, it is designed for biomolecular NMR, and is therefore our most versatile instrument, with four channels instead of two; waveform generators on all four channels; pre-cooling of the VT air to permit low-temperature studies; and a variety of probes. Recently it has also been used to study more "exotic" nuclei, such as 11B, 119Sn, and129Xe.

Inova 500
- 4 full channels
- short 2H pulse
- NOESY, ROESY, TOCSY…HMQC, HSQC, HMBC…
- triple resonance experiments (1H, 13C, 15N, 31P)
- relaxation experiments
- Probes:
5mm triple resonance XYZ
5mm broadband PFG
5mm Penta PFG
3mm triple resonance PFG

Inova 400
- 1H & 13C detect
- 1H & 13C decouple
- 1D experiments: APT, DEPT, INEPT…
- 2D experiments: NOESY, COSY, DQFC, HSQC, HMBC…

400MR Spectrometer
- 1H, 13C, 31P, 19F experiments
- 2D experiments: NOESY, COSY, DQFC, HSQC, HMBC…
- automated
- SMS 100 sample changer (robot)
- dual ProTune for automatic tuning
- automated locking
- gradient shimming

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Nuclear magnetic resonance spectroscopy
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The Facility has two 400 MHz instruments: an Inova 400 and an automated 400MR. Both are used for 1H and 13C 1D experiments (the 400MR can also observe 19F and 31P) and those 2D experiments often associated with small molecules (e.g., COSY, ROESY, HSQC, HMBC, etc.). The Laboratory also has an Inova 500. This is now primarily used... Show more »

The Facility has two 400 MHz instruments: an Inova 400 and an automated 400MR. Both are used for 1H and 13C 1D experiments (the 400MR can also observe 19F and 31P) and those 2D experiments often associated with small molecules (e.g., COSY, ROESY, HSQC, HMBC, etc.). The Laboratory also has an Inova 500. This is now primarily used to study small molecules, but was originally designed for biomolecular NMR. It is therefore our most versatile instrument, with four channels instead of two; waveform generators on all four channels; pre-cooling of the VT air to permit low-temperature studies; and a variety of probes. Recently it has also been used to study more exotic nuclei, such as 11B and 129Xe.

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