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UCSF Nikon Imaging Center

San Francisco, California, US

The Nikon Imaging Center at UCSF/QB3 (http://nic.ucsf.edu/) is a core facility for light microscopy established in partnership with Nikon Instruments, Inc. and a number of other microscopy vendors to provide access to cutting-edge microscopy tools for both UCSF researchers and outside researchers.

We have seven microscopes available for use: three widefield systems (all with temperature and CO2 control for mammalian cell time lapse imaging); a spinning disk confocal; a laser-scanning confocal; and structured illumination and STORM super-resolution systems. We can do high speed imaging (100 fps for multiple colors and >400 fps for single color imaging). We also have deconvolution and image analysis software available.

In general, our scopes are user operated and we provide training and assistance, although we can acquire data from fixed slides for you

For more information, see http://nic.ucsf.edu/

UCSF Nikon Imaging Center has not listed any services.

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3D Structured Illumination Microscopy
Starting at $82.00 per hour

We have a Nikon N-SIM microscope, equipped with four laser lines (405, 488, 561, and 647 nm) that can acquire super-resolution images with a resolution of ~120 nm in XY. It is capable of 3D Z-stack acquisition and can also acquire 2D TIRF-SIM images.

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

We have a Nikon N-SIM microscope, equipped with four laser lines (405, 488, 561, and 647 nm) that can acquire super-resolution images with a resolution of ~120 nm in XY. It is capable of 3D Z-stack acquisition and can also acquire 2D TIRF-SIM images.

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

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Deconvolution Microscopy
Starting at $60.00 per hour

We can acquire and deconvolve 3D image stacks on any of our widefield microscopes.

We can acquire and deconvolve 3D image stacks on any of our widefield microscopes.

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Fluorescence-Based Microscopy
Starting at $60.00 per hour

This widefield microscope is optimized for high speed, large field-of-view imaging. It captures 5.5 megapixel images at up to 100 frames per second and can acquire Z-stacks at mutlicolor images at this speed. It can acquire smaller fields of view much faster (e.g. 512 x 512 pixels at 420 fps). It is capable of both fluorescence... Show more »

This widefield microscope is optimized for high speed, large field-of-view imaging. It captures 5.5 megapixel images at up to 100 frames per second and can acquire Z-stacks at mutlicolor images at this speed. It can acquire smaller fields of view much faster (e.g. 512 x 512 pixels at 420 fps). It is capable of both fluorescence imaging and color brightfield imaging. It has a motorized X-Y stage and can acquire images from multiwell plates and can also acquire tiled images of large samples for image stitching.

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Fluorescence Microscopy Training
Starting at $200.00 per hour

We provide training on any of the fluorescence microscopes in our facility, including advice on optimization of the microscope system for imaging your samples, and advice on sample preparation.

Internal Rates: $0 per hour
External Academic Rates: $128 per hour
External Corporate Rates: $200 per hour

We provide training on any of the fluorescence microscopes in our facility, including advice on optimization of the microscope system for imaging your samples, and advice on sample preparation.

Internal Rates: $0 per hour
External Academic Rates: $128 per hour
External Corporate Rates: $200 per hour

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Light Sheet Microscopy
Starting at $82.00 per hour

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

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Spectral Confocal Microscopy
Starting at $82.00 per hour

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

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Live Cell Imaging
Starting at $82.00 per hour

We have a fully automated Nikon Ti microscope equipped with a 37°C incubation chamber and CO2 control for live cell imaging. Nikon Perfect Focus keeps samples in focus over hours or days for automated time lapse experiments while the automated stage allows for multi-position imaging.

This microscope is equipped for imaging of... Show more »

We have a fully automated Nikon Ti microscope equipped with a 37°C incubation chamber and CO2 control for live cell imaging. Nikon Perfect Focus keeps samples in focus over hours or days for automated time lapse experiments while the automated stage allows for multi-position imaging.

This microscope is equipped for imaging of DAPI / FITC / Cy3 / Cy5 / CFP / YFP / GFP / RFP and differential interference contrast (DIC) and phase contrast imaging.

Time Lapse, Attended

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

Time Lapse, Unattended

Internal Rates: $4.65 per hour
External Academic Rates: $9.31 per hour
External Corporate Rates: $16.29 per hour

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Spinning Disk Confocal Microscopy
Starting at $82.00 per hour

This system is a Nikon Ti equipped with a Yokagawa CSU22 spinning disk confocal an XY stage, and a piezo Z-stage. It has four laser lines (405, 488, 561, 640 nm) and is capable of multi-position imaging and fast multicolor Z-stack acquisition, up to 60 frames per second. It has a CO2 and temperature controlled incubator for live... Show more »

This system is a Nikon Ti equipped with a Yokagawa CSU22 spinning disk confocal an XY stage, and a piezo Z-stage. It has four laser lines (405, 488, 561, 640 nm) and is capable of multi-position imaging and fast multicolor Z-stack acquisition, up to 60 frames per second. It has a CO2 and temperature controlled incubator for live specimen imaging.

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

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Stochastic Optical Reconstruction Microscopy
Starting at $80.00 per hour

We have a Nikon N-STORM microscope and can provide training and assistance operating it. We have high power (>100 mW) lasers at 488, 561, and 647 nm, and a 405 nm laser for dye activation. This system is capable of PALM and STORM microscopy.

We have a Nikon N-STORM microscope and can provide training and assistance operating it. We have high power (>100 mW) lasers at 488, 561, and 647 nm, and a 405 nm laser for dye activation. This system is capable of PALM and STORM microscopy.

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TIRF
Total Internal Reflection Microscopy
Starting at $82.00 per hour

A four color (405, 488, 561, and 647 nm) TIRF microscopy system, with motorized Z-stage and stage-top incubator for time lapse imaging of live cells. Please contact us for additional information.

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

A four color (405, 488, 561, and 647 nm) TIRF microscopy system, with motorized Z-stage and stage-top incubator for time lapse imaging of live cells. Please contact us for additional information.

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

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Widefield fluorescence microscopy
Starting at $82.00 per hour

Use of the 6D Widefield Microscope, a fully-motorized Nikon Ti equipped with an XY stage, excitation and emission filter wheels, a Coolsnap HQ2 CCD and a Nikon color CCD. Filters are available for imaging DAPI, FITC, Cy3, Cy5, Cy7, CFP, YFP, GFP, and RFP. Pricing is for outside industrial users; academic use is cheaper. We can... Show more »

Use of the 6D Widefield Microscope, a fully-motorized Nikon Ti equipped with an XY stage, excitation and emission filter wheels, a Coolsnap HQ2 CCD and a Nikon color CCD. Filters are available for imaging DAPI, FITC, Cy3, Cy5, Cy7, CFP, YFP, GFP, and RFP. Pricing is for outside industrial users; academic use is cheaper. We can image user provided samples on this microscope for an additional fee.

Internal Rates: $23 per hour
External Academic Rates: $47 per hour
External Corporate Rates: $82 per hour

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Imaging & Spectroscopy
Price on request
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Imaging and Microscopy Training
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Microscopy
Price on request
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Optical Microscopy
Price on request
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Confocal Microscopy
Price on request
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Super Resolution Microscopy
Price on request
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