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Translational Biodiscovery Lab, Christchurch Heart Institute

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Christchurch, NZ

About Translational Biodiscovery Lab, Christchurch Heart Institute

Divison of: University of Otago

Here at the Christchurch Heart Institute we help save thousands of lives and increase survival of those suffering from heart disease, through research into improved diagnosis, better prediction and advanced treatments.

Our research is focussed on

  • New blood tests for heart attack diagnosis and prognosis
  • Using new methods for treating heart failure and improving outcomes
  • Understanding the genetics of heart disease; why heart disease runs in some families

At the Translational Biodiscovery Lab, we focus on the development of novel biomarker assays for the diagnosis and prognosis of disease, in both clinical and pre-clinical settings. Most assays have been developed to detect the human, rodent and ovine forms of these hormones. In addition, HPLC technology is employed to assess the kinetics and mechanisms underlying the metabolism of peptides in plasma and tissue. The lab is well equipped with high-throughput Roche and BRAHMS Kryptor analysers, as well as RIA, ELISA and Luminex technologies. We also have in vitro cell culture and ex vivo isolated heart facilities for further biodiscovery applications.

Recent Publications

  • Ellmers, L. J., Rademaker, M. T., Charles, C. J., Yandle, T. G., & Richards, A. M. (2015). (PRO)renin receptor blockade ameliorates cardiac injury and remodeling and improves function after myocardial infarction. Journal of Cardiac Failure. Advance online publication.
  • Yandle, T. G., & Richards, A. M. (2015). B-Type natriuretic peptide circulating forms: Analytical and bioactivity issues [Invited critical review]. Clinica Chimica Acta, 448, 195-205.
  • Lampinen, K. H., Rönnback, M., Groop, P.-H., Nicholls, M. G., Yandle, T. G., & Kaaja, R. J. (2014). Increased plasma norepinephrine levels in previously pre-eclamptic women. Journal of Human Hypertension, 28(4), 269-273.
  • Mahagamasekera, P. G., Ruygrok, P. N., Palmer, S. C., Richards, A. M., Ansell, G. S., Nicholls, M. G., Pemberton, C. J., Lewis, L. K., Yandle, T. G. (2014). B-type natriuretic peptide forms within the heart, coronary sinus, and peripheral circulation in humans: Evidence for degradation before secretion. Clinical Chemistry, 60(3), 549-558.
  • Lewis, L. K., Yandle, T. G., Hilton, P. J., Jensen, B. P., Begg, E. J., & Nicholls, M. G. (2014). Endogenous ouabain is not ouabain. Hypertension, 64, 680-683.
  • Cullen, L., Müller, C., Parsonage, W. A., Wildi, K., Greenslade, J. H., Twerenbold, R., Aldous, S., … Troughton, R., … George, P., … Pemberton, C. J., Richards, A. M., … Florkowski, C., Deely, J. M., Than, M. (2013). Validation of high-sensitivity troponin I in a 2-hour diagnostic strategy to assess 30-day outcomes in emergency-department patients with possible acute coronary syndrome. JACC, 62(14), 1242-1249.
  • Pemberton, C. J., Siriwardena, M., Kleffmann, T., Ruygrok, P., Palmer, S. C., Yandle, T. G., & Richards, A. M. (2012). First identification of circulating Prepro-A-Type natriuretic peptide (PreproANP) signal peptide fragments in humans: Initial assessment as cardiovascular biomarkers. Clinical Chemistry, 58(4), 757-767.

Our Services (25)


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Clinical Biomarkers

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Biomarker Analysis

Starting at $13.00 per sample

Cardiovascular biomarker research measurement and biochemical analysis of over 30 different important markers (including troponins, BNP/ANP/CNP peptides, neprilysin activity, endothelins, angiotensin, catecholamines) and more. All assays are quality controlled to ISO15189-like standard and we have undertaken contracts for multiple research and industry providers.

LC-MS/MS Luminex® ELISA

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Assay Development

Price on request

Assays that have been developed within the laboratory include;

  • atrial natriuretic peptide (ANP)
  • brain natriuretic peptide (BNP)
  • N-terminal BNP
  • c-type natriuretic peptide (CNP)
  • neutral endopeptidase activity (NEP or E.C. 24.11)
  • adrenomedullin
  • angiotensin II
  • angiotensin converting enzyme activity (ACE)
  • plasma renin activity
  • arginine vasopressin
  • ACTH
  • cortisol
  • aldosterone
  • endothelin
  • cGMP
  • cAMP
  • the catecholamines
  • Ghrelin peptides
  • signal peptide fragments derived from preproproteins


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HPLC-MS/MS

High Performance Liquid Chromatography Coupled Tandem Mass Spectrometry
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Biochemical characterisation of targets can be carried out using reverse phase, size exclusion and ion exchange HPLC modalities. Mass spectroscopy analysis to augment results can also be arranged.


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Luminex Multiplex Assays

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In house multiplex assays to differential BNP peptide forms and singleplex for CNP peptides are available. In house assays for ANP peptides are in development. Externally provided cytokine, growth factor and cardiovascular markers are available on request.


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ELISA

Enzyme-linked immunosorbent assay
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A full suite of tissue and plasma ELISAs are available for cardiovascular, metabolic, immunology and emergency medicine studies in clinical and experimental settings.


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Biology

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Clinical Chemistry

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Biochemistry & Molecular Biology

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Mass Spectrometry

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Liquid Chromatography Coupled Mass Spectrometry Methods

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Project Management

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Clinical Laboratory Services

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Clinical Research

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Pharmacology & Toxicology

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Protein Purification

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Protein Purification and Quantification Services


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Drug Discovery & Development

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Drug Discovery & Development Services


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Pharmacology

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Pharmacology Services


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Imaging & Spectroscopy

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Antibody/Antigen Detection Based Testing

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Protein Services

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Spectroscopy

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Biomarkers

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Protein Quantification

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Bioanalysis

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CP

Chris Pemberton

Research Associate Professor

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