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Transgenics and Genetic Constructs Shared Resource (TGCSR)

Lebanon, New Hampshire, US

The mission of the TGCSR is to support the generation and utilization of genetically modified mice by members of the Dartmouth research community. The support starts with intellectual support in planning and designing transgenic experiments provided by consultation with the TGCSR director. The TGCSR is now able to offer not only assistance with design of genetic constructs but also the production of genetic constructs for use in transgenic animal production through “recombineering” in either yeast or E. coli. The tasks of manipulating the mouse embryo are fully provided by the TGCSR either in house or through an established relationship with the Jackson Labs. Jackson labs is now providing cryopreservation services to protect and preserve valuable transgenic mouse lines. The TGCSR can also provide genotyping and mouse husbandry services. As currently organized, the TGCSR can provide the full range of services... Show more »

The mission of the TGCSR is to support the generation and utilization of genetically modified mice by members of the Dartmouth research community. The support starts with intellectual support in planning and designing transgenic experiments provided by consultation with the TGCSR director. The TGCSR is now able to offer not only assistance with design of genetic constructs but also the production of genetic constructs for use in transgenic animal production through “recombineering” in either yeast or E. coli. The tasks of manipulating the mouse embryo are fully provided by the TGCSR either in house or through an established relationship with the Jackson Labs. Jackson labs is now providing cryopreservation services to protect and preserve valuable transgenic mouse lines. The TGCSR can also provide genotyping and mouse husbandry services. As currently organized, the TGCSR can provide the full range of services required to design, generate and maintain transgenic mouse lines.

TGCSR currently maintains two specialized transgenic lines that express Cre or Flp in the germline for genetic manipulation of transgene/knockouts in vivo. TGCSR has a fully equipped embryo manipulation laboratory in the Borwell animal facility and an adjoining 4 room suite of mouse husbandry rooms.

We generally get cell lines from researchers who order. Many of the studies currently are primary tumor cells from patients in what is known as Patient Derived Xenografts (PDX). We do a lot of that as well. We have access to a variety of human melanoma, ovarian, brain and breast lines and likely can find many others from other tumor types within Dartmouth if we send out a request for them.

We have our own colony of NSG mice that we keep for making xenografts of hemopoietic stem cells to make mice with humanized immune systems, one of our strengths. We also do the tumor xenografts in these mice. If someone wants xenografts done in nudes or Rags or something else that is immunodeficient for some reason we order those mice from JAX.

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Transgenics and Genetic Constructs Shared Resource (TGCSR) has not listed any services.

Recombineering
Recombination-Mediated Genetic Engineering
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Southern Blot
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SNP Genotyping
Single Nucleotide Polymorphism Genotyping
Price on request

We offer background checks to verify the validity of your mice population. Confirming the genetic background and purity of your mice DNA gives you peace of mind as you submit manuscripts for publication, or grant applications for review.

We offer background checks to verify the validity of your mice population. Confirming the genetic background and purity of your mice DNA gives you peace of mind as you submit manuscripts for publication, or grant applications for review.

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Mouse
Congenic Strain Creation
Price on request

Speed Congenic mouse lines are created by introgressing genomic intervals (typically containing a modified gene such as a knockout, knockin, or transgene) from one strain (donor strain) to another (recipient strain) using a marker assisted breeding strategy. This is achieved by sequentially back ‐ crossing donor strain mice... Show more »

Speed Congenic mouse lines are created by introgressing genomic intervals (typically containing a modified gene such as a knockout, knockin, or transgene) from one strain (donor strain) to another (recipient strain) using a marker assisted breeding strategy. This is achieved by sequentially back ‐ crossing donor strain mice containing your genetic region of interest to mice of the recipient strain. At each generation, multiple offspring mice are analyzed at the genomic DNA level, using single nucleotide polymorphisms (SNPs) spread throughout the genome, so as to select the “best” mouse (i.e. most similar to the recipient strain) to breed for the next generation, accelerating the development of the congenic mouse line. This is the basis for the SPEED aspect of the Speed Congenic approach.

To maximize the speed and efficiency with which you can develop your Congenic strain, DartMouse recommends sending us samples from male mice that are carriers (i.e. heterozygotes) of your gene/region of interest. Since you can cycle‐ breed males (or place multiple females with each male) selecting males to breed will allow you to generate the desired number of pups at each generation, since the optimal number for us to screen at each generation is 10 male carriers. This
means you should plan to generate at least 40 pups at each generation after the N1 generation, a
number arrived at by keeping in mind the following considerations: approximately 50% of pups born will be males, and approximately 50% will be carriers of your gene of interest. Therefore, about 25% of mice raised in your colony will be both males as well as carriers (heterozygotes) of the modified allele of your gene locus of interest. This is a theoretical calculation, of course, and real ‐ world numbers will vary. Therefore, sometimes you will generate fewer than 10 carriers and sometimes greater. Keep in mind that the fewer mice that DartMouse screens per generation, the lower the benefit of the Speed Congenic approach. Screening four or fewer mice per generation is generally not recommended. Conversely, the cost ‐benefit ratio of screening greater than 11 male carriers begins to increase significantly, markedly reducing the cost‐ effectiveness of the Speed Congenic approach, so we do not advise this for our clients. Thus, we recommend a “sweet spot” target of 10 male carriers.

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Animal Husbandry
Price on request
  • timed pregnancies
  • superovulations
  • maintain transgenic colonies (husbandry)
  • maintaining Flp and Cre expressing mice for mating to transgenic
  • timed pregnancies
  • superovulations
  • maintain transgenic colonies (husbandry)
  • maintaining Flp and Cre expressing mice for mating to transgenic
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Animal Surgery
Price on request
  • tail biopsies
  • tail vein injections
  • ovariectomies
  • vasectomies
  • embryo transfer
  • blood drawing
  • tail biopsies
  • tail vein injections
  • ovariectomies
  • vasectomies
  • embryo transfer
  • blood drawing
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BAC Transgenic Mouse Production
Price on request
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Blastocyst Injection
Price on request
  • Generation of chimeric mice from ES cells
  • Inject ES cells into blastocysts to generate germline chimeric mice
  • Generation of chimeric mice from ES cells
  • Inject ES cells into blastocysts to generate germline chimeric mice
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Embryonic Stem Cell (ESC) Culture
Price on request

ES cell culture for gene targeting or other purposes.

ES cell culture for gene targeting or other purposes.

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Embryonic Stem Cell Gene Targeting
Price on request
  • provide a variety of ES cell lines
  • transfect with KO constructs
  • select clones with drug selection
  • freeze and provide lysates for genotyping
  • expand and refreeze targeted clones for mouse generation
  • karyotype clones to select best clones
  • provide a variety of ES cell lines
  • transfect with KO constructs
  • select clones with drug selection
  • freeze and provide lysates for genotyping
  • expand and refreeze targeted clones for mouse generation
  • karyotype clones to select best clones
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Humanized Mouse Models
Price on request

Humanized mouse:
- Creating specialized mice with a human immune system.
- Currently offering two different techniques using Nod/SCIDIL2 gamma receptor XO mice.

Humanized mouse:
- Creating specialized mice with a human immune system.
- Currently offering two different techniques using Nod/SCIDIL2 gamma receptor XO mice.

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Immunodeficient Animal Models
Price on request
  • Creating specialized mice with a human immune system.
  • Currently offering two different techniques using Nod/SCIDIL2 gamma receptor XO mice.
  • Creating specialized mice with a human immune system.
  • Currently offering two different techniques using Nod/SCIDIL2 gamma receptor XO mice.
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Rodent Rederivation
Price on request

Establish lines from frozen embryos from another institutions or rederive a pathogen infected transgenic line by embryo transfer

Establish lines from frozen embryos from another institutions or rederive a pathogen infected transgenic line by embryo transfer

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Mouse
Animal Embryo and Sperm Cryopreservation
Price on request
  • Cryopreservation of embryos and sperm
  • To preserve & protect your valuable transgenic mouse lines
  • Cryopreservation of embryos and sperm
  • To preserve & protect your valuable transgenic mouse lines
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Patient-Derived Xenograft (PDX)
Price on request

Many of the studies currently are primary tumor cells from patients in what is known as Patient Derived Xenografts (PDX). We do a lot of that as well. We have access to a variety of human melanoma, ovarian, brain and breast lines and likely can find many others from other tumor types within Dartmouth if we send out a request for them.

Many of the studies currently are primary tumor cells from patients in what is known as Patient Derived Xenografts (PDX). We do a lot of that as well. We have access to a variety of human melanoma, ovarian, brain and breast lines and likely can find many others from other tumor types within Dartmouth if we send out a request for them.

« Show less
Transgenic Genotyping
Price on request
  • Purify DNA from ES clones (to screen for targeted clones) or tail biopsies (to screen for founders or transgenic offspring).
  • Establish a genotype assay if not already established.
  • Genotype by PCR or Southern blot
  • Purify DNA from ES clones (to screen for targeted clones) or tail biopsies (to screen for founders or transgenic offspring).
  • Establish a genotype assay if not already established.
  • Genotype by PCR or Southern blot
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Genetically Engineered Mice
Price on request

Provide consultation on transgenic mice:
- construct design and production
- overall experimental design
- utilization
- maintenance
- breeding
- preservation

Genetic construct generation:
- Use recombineering in E. coli or yeast to generate constructs for experimental use

Generate transgenic founders by injection of... Show more »

Provide consultation on transgenic mice:
- construct design and production
- overall experimental design
- utilization
- maintenance
- breeding
- preservation

Genetic construct generation:
- Use recombineering in E. coli or yeast to generate constructs for experimental use

Generate transgenic founders by injection of plasmids, BAC, or lentivirus into oocytes and transplant the oocytes in foster mothers

« Show less
Human Tumor Xenograft Models
Price on request

We have our own colony of NSG mice that we keep for making xenografts of hemopoietic stem cells to make mice with humanized immune systems, one of our strengths. We also do the tumor xenografts in these mice. If someone wants xenografts done in nudes or Rags or something else that is immunodeficient for some reason we order... Show more »

We have our own colony of NSG mice that we keep for making xenografts of hemopoietic stem cells to make mice with humanized immune systems, one of our strengths. We also do the tumor xenografts in these mice. If someone wants xenografts done in nudes or Rags or something else that is immunodeficient for some reason we order those mice from JAX.

We generally get cell lines from the researchers ordering from us. Many of the studies currently are primary tumor cells from patients in what is known as Patient Derived Xenografts (PDX). We do a lot of that as well. We have access to a variety of human melanoma, ovarian, brain and breast lines and likely can find many others from other tumor types within Dartmouth if we send out a request for them.

« Show less
Nucleic Acid Services
Price on request
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Genetic Engineering
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DNA Services
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Oncology Animal Models
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Biology
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Mammalian Cell Culture
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Biochemistry & Molecular Biology
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Transgenic Mouse Services
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Animal Models and Studies
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Animal Model in vivo Analyses
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Animal Biospecimens
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Transgenic Animal Model Development
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Animal Model Housing and Maintenance
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DNA Fragment Analysis
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Animal Models of Disease
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Cells and Tissues
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Xenograft Models
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Genotyping Services
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Immunology Animal Models
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Biospecimens
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Animal Physiology Analyses
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