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Transgenic Mouse Models

Portland, Oregon, US

Transgenic Mouse Models Core laboratory (TMM) is a structural unit of the OHSU Shared Resource Program and is supported by this program and the Knight Cancer Institute at OHSU.

The Transgenic Core was established within OHSU in 1994 in response to a growing interest and need for this important technology of many scientists and physicians among different faculties of OHSU.

There has been exponential growth of transgenic mouse projects at OHSU with over two hundred fifty unique transgenic and knockout mouse lines produced during the past 17 years. Moreover, the genetically modified mice generated by OHSU's Transgenic Core are successfully used by investigators within the USA and other countries. Currently the TMM supports 27 grants from NIH and other funding agencies.

Service fees include:

1) Purchase of virgin C57BL6/J or B6xDBA-2F1 females (donors of oocytes).
2) Superovulation of the virgin... Show more »

Transgenic Mouse Models Core laboratory (TMM) is a structural unit of the OHSU Shared Resource Program and is supported by this program and the Knight Cancer Institute at OHSU.

The Transgenic Core was established within OHSU in 1994 in response to a growing interest and need for this important technology of many scientists and physicians among different faculties of OHSU.

There has been exponential growth of transgenic mouse projects at OHSU with over two hundred fifty unique transgenic and knockout mouse lines produced during the past 17 years. Moreover, the genetically modified mice generated by OHSU's Transgenic Core are successfully used by investigators within the USA and other countries. Currently the TMM supports 27 grants from NIH and other funding agencies.

Service fees include:

1) Purchase of virgin C57BL6/J or B6xDBA-2F1 females (donors of oocytes).
2) Superovulation of the virgin females.
3) Microinjection into 250-300 C57BL/6 or B6xDBA-2 zygotes.
4) Transplantation of the microinjected embryos into pseudopregnant recipient females.
5) Animal housing charges for recipients and their offspring until weaning.
6) The production of 3 guaranteed transgenic founders. TgMM does not guarantee expression of the transgene.

Service fees do not include:

1) Animal housing charges for litters after weaning.
2) Ear/tail tissue collection for DNA isolation for genotyping.
3) Health testing of the foster mother.
4) Shipping charges.

Additional Services:
According to the customers' request, TgMM can purchase and use other mouse lines for the generation of genetically modified mice. Moreover, TgMM can tag, collect tail samples, extract DNA and genotype the offspring by PCR. For this purpose the animals can be housed in our colony beyond the usual 3 weeks (weaning age). These additional services will be charged as extra fees, including the extra costs for the cage(s) per diem.

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Transgenic Mouse Models has not listed any services.

Blastocyst Injection
Starting at $1,500.00 per sample

1 clone $2,350 103 A
2 clones $4,700 103 B
One day injection $1,500 103 A1

1 clone $2,350 103 A
2 clones $4,700 103 B
One day injection $1,500 103 A1

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Cryopreservation
Starting at $100.00 per sample

Cryo Storage of ES cells 1 line/year $100 111 A

Cryo Storage of ES cells 1 line/year $100 111 A

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Embryonic stem cell culture
Embryonic stem cell culture
Starting at $550.00 per sample

102 ES Cell Gene Targeting $3,900
102 A Expanding ES cell clones from 96-well plates:
102 A11 clone $550
102 A22 clones $700
102 A3 3 clones $850
$150 for every additional clone, up to 10 clones (102 A10)
102 B Expand one vial of ES cells to freeze back $500

102 ES Cell Gene Targeting $3,900
102 A Expanding ES cell clones from 96-well plates:
102 A11 clone $550
102 A22 clones $700
102 A3 3 clones $850
$150 for every additional clone, up to 10 clones (102 A10)
102 B Expand one vial of ES cells to freeze back $500

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Mouse Embryonic Fibroblast Feeder
Starting at $250.00 per sample

MEF feeder cells (1 vial, 5x106 cells,) $250 (120 C)

MEF feeder cells (1 vial, 5x106 cells,) $250 (120 C)

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Animal Embryo and Sperm Cryopreservation
Starting at $100.00 per sample

Cryopreservation of embryos $1,600 110 E
Quality control of frozen embryos by retransfer in uterus $200 105
Cryo Storage of embryos/sperm 1 line/year $100 111
Cryopreservation of sperm $500... Show more »

Cryopreservation of embryos $1,600 110 E
Quality control of frozen embryos by retransfer in uterus $200 105
Cryo Storage of embryos/sperm 1 line/year $100 111
Cryopreservation of sperm $500 110 S

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Rodent Rederivation
Starting at $500.00 per sample

Rederivation via Embryo Transfer $500 (104 A )
Rederivation via IVF w/ Frozen-Thawed Sperm $1,500 (104 B)

Rederivation via Embryo Transfer $500 (104 A )
Rederivation via IVF w/ Frozen-Thawed Sperm $1,500 (104 B)

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Mouse
Pronuclear injection
Starting at $1,600.00 per sample

101 B B6D2F1 hybrid $3,600
101 B1 B6D2F1 hybrid $1,600 One day injection
101 CC57BL/6 $4,800
101 C1C57BL/6 $2,000 One day injection

101 B B6D2F1 hybrid $3,600
101 B1 B6D2F1 hybrid $1,600 One day injection
101 CC57BL/6 $4,800
101 C1C57BL/6 $2,000 One day injection

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Genetically Engineered Mice
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Genetic Engineering
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Mammalian Cell Culture
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Transgenic Mouse Services
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Biospecimens
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Animal Housing and Maintenance
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Biology
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Cryostorage
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Animal Models and Studies
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Animal Biospecimens
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Transgenic Animal Model Development
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Cells and Tissues
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Human Biospecimen Management and Storage
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