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Transgenic & Gene Targeting Core

Salt Lake City, Utah, US

The Transgenic and Gene Targeting Mouse Core is available to make transgenic mice using pronuclear injection of DNA constructs including BAC DNA and CRISPR's into embryos. The Core is available to generate gene-targeted mice using knockout or conditional constructs electroporated into embryonic stem (ES) cells. This is followed by blastocyst injection of targeted ES cells to obtain germline chimeric mice eventually leading to the production of gene-targeted mice. The Transgenic and Gene Targeting Mouse Core offers related procedures including cryopreservation and storage of mouse sperm and mouse embryos, rederivation of mouse lines from frozen embryos, in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to rederive frozen sperm, development of primary ES cell lines from targeted mouse lines, and karyotyping of ES clones.

The Core is available for technical advice regarding injection... Show more »

The Transgenic and Gene Targeting Mouse Core is available to make transgenic mice using pronuclear injection of DNA constructs including BAC DNA and CRISPR's into embryos. The Core is available to generate gene-targeted mice using knockout or conditional constructs electroporated into embryonic stem (ES) cells. This is followed by blastocyst injection of targeted ES cells to obtain germline chimeric mice eventually leading to the production of gene-targeted mice. The Transgenic and Gene Targeting Mouse Core offers related procedures including cryopreservation and storage of mouse sperm and mouse embryos, rederivation of mouse lines from frozen embryos, in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to rederive frozen sperm, development of primary ES cell lines from targeted mouse lines, and karyotyping of ES clones.

The Core is available for technical advice regarding injection procedures, cell culture techniques, vector design and construction, as well as analysis of ES cell clones and mice. The Core is amenable to new ideas and available to try new methods that researchers are interested in. The Core maintains the necessary mouse colonies for basic procedures, including a large colony of C57Bl6 mice, albino C57Bl6 mice, C57Bl6 x CBA F1 mice. We also maintain a small colony of ROSA-flp mice in the C57Bl6 background, and CMV-cre deleter mice. The Core maintains 129 ES cells, 129/C57Bl6 hybrid ES cells, and C57Bl6 ES cells (N and J) for gene-targeting. Equipment includes two full microinjection stations and dissection microscopes with fluorescence imaging capabilities, Peizo drills, Femtojet injectors, an XYClone laser, pipette pullers, microforges, and a rate controlled embryo freezer.

The Core has successfully provided genetically altered mice to many University of Utah researchers as well as to researchers throughout the world. The Core has rederived mouse lines for researchers using frozen sperm and frozen embryos shipped from around the world.


Certifications: IACUC


Available animals:
- C57Bl6 mice
- albino C57Bl6 mice
- C57Bl6 x CBA F1 mice
- ROSA-flp mice in C57Bl6 background
- CMV-cre deleter mice


Cells available:
- 129 ES cells
- 129/C57Bl6 hybrid ES cells
- C57Bl6 ES cells (N and J) for gene-targeting


Equipment:
- two full microinjection stations and dissection microscopes with fluorescence imaging capabilities
- Peizo drills
- Femtojet injectors
- XYClone laser
- pipette pullers
- microforges
- rate controlled embryo freezer


Standard sample shipment requests:
- sperm and embryos must be shipped in liquid nitrogen dry shipper
- cells must be sent on dry ice, overnight


We work closely with the University of Utah Mutation Generation and Detection Core in the design and construction of CRISPR's. Link is https://www.scienceexchange.com/labs/mutation-generation-and-detection-facility.

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Transgenic & Gene Targeting Core has not listed any services.

Pronuclear Injection
Starting at $4,500.00 per prep

Production of transgenic mice using pronuclear injection of DNA, including BAC DNA and CRISPR's. At least 200 embryos will be injected and implanted. Ear biopsies from all resulting pups will be given to the investigator for genotyping.
Pricing for University of Utah labs is less.

Production of transgenic mice using pronuclear injection of DNA, including BAC DNA and CRISPR's. At least 200 embryos will be injected and implanted. Ear biopsies from all resulting pups will be given to the investigator for genotyping.
Pricing for University of Utah labs is less.

« Show less
Rodent in vitro Fertilization
Starting at $1,500.00 per sample

In vitro fertilization to rederive mouse lines from frozen or fresh sperm.
Pricing is less for University of Utah investigators.

In vitro fertilization to rederive mouse lines from frozen or fresh sperm.
Pricing is less for University of Utah investigators.

« Show less
Blastocyst Injection
Starting at $8,250.00 per cell line

Targeted ES cells (two clones from the same targeting construct) will be microinjected into C56Bl/6 blastocysts, after which they will be implanted into pseudopregnant females. Any resulting male pups which are >40% chimeric will be bred to test for germline transmission. It is recommended that targeted ES clones by karyotyped... Show more »

Targeted ES cells (two clones from the same targeting construct) will be microinjected into C56Bl/6 blastocysts, after which they will be implanted into pseudopregnant females. Any resulting male pups which are >40% chimeric will be bred to test for germline transmission. It is recommended that targeted ES clones by karyotyped prior to microinjection.
Price for University of Utah labs is lower.

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Embryonic Stem Cell Gene Targeting
Starting at $7,500.00 per prep

Production of gene targeted embryonic stem cells using selectable marker(s). We target your DNA into 129 derived R1 ES cells or G4 ES cells (129/C57B16 hybrid) or C57Bl6 ES cells, either N or J.
The gene-targeting facility will electroporate DNA provided by the investigator into embryonic stem cells (ES cells) and perform... Show more »

Production of gene targeted embryonic stem cells using selectable marker(s). We target your DNA into 129 derived R1 ES cells or G4 ES cells (129/C57B16 hybrid) or C57Bl6 ES cells, either N or J.
The gene-targeting facility will electroporate DNA provided by the investigator into embryonic stem cells (ES cells) and perform positive-negative selection. At least 100 ES clones will be picked, frozen, and duplicate plates will be grown up for DNA. DNA will be isolated and given to the investigator to analyze for targeted clones.

Guaranteed 100 or more clones for you to analyze. Price includes growing up and refreezing targeted clones. Price is less for University of Utah labs.

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Transgenic Animal Model Development
Starting at $4,500.00 per prep

Production of knockout or gene targeted mice using CRISPR/Cas technology. At least 200 embryos will be injected and implanted. Ear biopsies from all resulting pups will be given to the investigator for genotyping.
We work closely with the University of Utah Mutation Generation and Detection Core in the design and construction... Show more »

Production of knockout or gene targeted mice using CRISPR/Cas technology. At least 200 embryos will be injected and implanted. Ear biopsies from all resulting pups will be given to the investigator for genotyping.
We work closely with the University of Utah Mutation Generation and Detection Core in the design and construction of CRISPR's. Link is https://www.scienceexchange.com/labs/mutation-generation-and-detection-facility.
Pricing for University of Utah labs is less.

« Show less
Genetically Engineered Mice
Starting at $4,500.00 per prep

Production of transgenic mice using pronuclear injection of DNA, including BAC DNA and CRISPR's. At least 200 embryos will be injected and implanted. Ear biopsies from all resulting pups will be given to the investigator for genotyping.
Pricing for University of Utah labs is less.

Production of transgenic mice using pronuclear injection of DNA, including BAC DNA and CRISPR's. At least 200 embryos will be injected and implanted. Ear biopsies from all resulting pups will be given to the investigator for genotyping.
Pricing for University of Utah labs is less.

« Show less
Animal Models and Studies
Price on request
Request a quote for more information about this service.
Animal Model Housing and Maintenance
Price on request
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Biology
Price on request
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Genetic Engineering
Price on request
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Transgenic Mouse Services
Price on request
Request a quote for more information about this service.
2018-04-06 12:00:15 -0700

Net Promoter Score of 10 received for Genetically Engineered Mice.

Additional Ratings: satisfaction with timeliness: 7.

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