TopQ BioSystems

We perform IHC image analysis on nuclei, membrane and cytoplasmic quantification (expression level score, stain intensity, percentage positive and etc.) for regular or TMA IHC data.

The price for histology image analysis: $50/hour

The sample estimate template for n hours of image analysis: n x $50

We perform immunofluorescent IHC double or triple staining on FFPE slides.

The prices for staining per slide are:
$35/slide (double staining)
$45/slide (triple staining)

The sample estimate template for n slides with double staining: n x $35

The sample estimate template for n slides with triple staining: n x $45

Deliverables:
- images
- image analysis data (if requested analysis)
- presented in Microsoft power point until unless requested by client in any other method

Capabilities:
- preparation and mounting of sectioned tissue from fixed tissues
- preparation of both paraffin-embedded and frozen sections
- have access to FFPE tissues in house: mouse tissue FFPE samples derived from the mice injected with human breast cancer cell lines.
- prepare sample sections for IHC

Data analyses available:
- We are able to perform quantitative data analysis on colorimetric staining, including statistics analysis.

We do not:
- have access to a tissue bank
- culture mammalian cells and prepare them for antibody staining
- culture non-mammalian (i.e. insect) cells
- transfect cells with either viruses or chemicals
- imaging of live cells

Equipment:
- Upright
- Inverted

Fluorescent channels and wavelengths:
- n/a - do not do fluorescent staining

Quality IHC for Difficult Targets,
as well as for those common and easy antigens.

We perform the IHC detections of difficult-to-detect target antigens in tissue and cell samples. Some target antigens are difficult-to-detect because of various reasons; the troubleshooting would be too time-consuming to meet the deadlines for the required quality IHC data, and also very costly for materials and labors. Confidently, we particularly provide the quality IHC services for those difficult targets in the research/ development projects. Our head technical expert has intensively been working on IHC for over 10 years with more than 300 primary antibodies, detected numerous antigens, including many difficult targets, no failed case so far. Therefore, we had rich experience in handling difficult staining to detect specific target signals, and constantly generating high-quality IHC data. We offer the following quality IHC services with the primary antibodies provided by customers:

-Primary antibody selections
-Formalin fixed paraffin embedded (FFPE) tissue sample sectioning
-Processing condition optimizations
-IHC single, double or triple staining (colorimetric or fluorescent)
FFPE slides
TMA slides
-IHC data analysis

Meanwhile, we also provide quality IHC services for those common and easy antigens.

The price for staining per slide: $25/slide (single staining)

The sample estimate template for n slides with single staining: n x $25

For single tissue samples (each tissue sample on a single slide)
Condition optimization: $250/antibody x 2 antibodies = $500
IHC staining: $25/slide x 60 slides = $1500

For TMA samples (60 samples on a single slide)
Condition optimization: $300/antibody x 2 antibodies = $600
TMA IHC staining: $40/slide x 2 slides = $80

We offer consulting services for colorimetric or fluorescent IHC single or double staining, including strategies, experimental designs, antibody selections, sample preparations, IHC data analysis and etc.

The price for consulting services: $50/hour

The sample estimate template for n hours of consulting services: n x $50

We perform IHC staining on Tissue microarray (TMA) slides.

The price for staining per TMA slide: $40/slide

The sample estimate template for n TMA slides: n x $40

We perform Formalin fixed paraffin embedded (FFPE) tissue sample sectioning for processing condition optimization tests.

The price for sectioning: $3/section

The sample estimate template for n sections: n x $3