Tissue Solutions Ltd

We provide a range of fresh human tissue samples to our clients in the UK, Europe and USA on a prospective basis. The viability of this will obviously vary depending on the locations of both the source and the client.
We can deliver a range of both normal and cancer samples from surgical resection for use in for example ex vivo and cell based assays.

In addition, post mortem samples can be provided from organ donors that are not suitable for transplantation.
Fresh bloods and urine samples can also be obtained from healthy and diseased volunteers on a prospective collection basis.

Fresh human skin is available for use in your drug discovery and development applications such as drug permeation studies, skin irritation and toxicology models and for the isolation of primary and stem cells.

We provide surgical excess tissue following cosmetic procedures and can supply sheets of fresh skin from abdomen (full thickness and dermatomed), breast, scalp, and occasionally from arm, upper and lower eye lid and back.

Post mortem skin can also be obtained from most areas of the body (depending on donor consent) and the typical post mortem intervals (PMIs) are 6-8 hours. The donor age range does vary but donors over the age of 65 will be more common.

All fresh skin samples can be shipped in your medium of choice and are supplied with details of the donors HCV, HBV and HIV status.

Cryopreserved human PBMCs and Cord Blood Mononuclear Cells are now available immediately, with functionality comparable to fresh isolations. We also offer highly purified monocyte, T-cells and B-cell subsets. These are available with basic or enhanced Characterization.

PBMCs available from normal and diseased donors (includes autoimmune diseases: RA, SLE,
Crohn’s, MS)
Basic Characterization — includes age, gender, ethnicity, HLA-I data
Enhanced Characterization — includes age, gender, ethnicity, HLA Class-I and Class-II data and ELISpot-IFNγ responses to PHA, CEF and CMV peptide pools
All cells are supplied with donor information including age, sex, liver pathology and medical history. We can also supply subcellular fractions: microsomes, S9 fractions (non—induced or induced and cytosols).

We are continually adding to our range of cells isolated from fresh human tissue. These are a valuable, physiologically relevant in vitro research tool. We can offer:

• Primary Cells and cell lines
  
• Normal/healthy human cells
  
• Disease specific human cells
  

These can be shipped as live cultures or cryopreserved.

Hepatocytes:

• Fresh or Frozen,
  
• For growing in suspension or in monolayers,
  
• As short— (5 to 6 days) or long—term cultures (up to 3 weeks).
  
• Fresh cells can be shipped in flasks or multiwall plates.
  

All cells are supplied with donor information including age, sex, liver pathology and medical history. We can also supply subcellular fractions: microsomes, S9 fractions (non—induced or induced and cytosols).

Peripheral Blood Mononuclear Cells (PBMC):

Cryopreserved human PBMCs and Cord Blood Mononuclear Cells are now available immediately, with functionality comparable to fresh isolations. We also offer highly purified monocyte, T-cells and B-cell subsets. These are available with basic or enhanced Characterization.

• PBMCs available from normal and diseased donors (includes autoimmune diseases: RA, SLE,
  Crohn’s, MS)
  
• Basic Characterization — includes age, gender, ethnicity, HLA-I data
  
• Enhanced Characterization — includes age, gender, ethnicity, HLA Class-I and Class-II data and ELISpot-IFNγ responses to PHA, CEF and CMV peptide pools
  

All cells are supplied with donor information including age, sex, liver pathology and medical history. We can also supply subcellular fractions: microsomes, S9 fractions (non—induced or induced and cytosols).

Human Keratinocytes:

Normal adult keratinocytes prepared from breast or abdominal skin.

Human Fibroblasts:

Isolated from abdominal skin, breast skin or scalp samples.

Human Melanocytes:

Epidermal melanocytes isolated from the skin of healthy donors.

Human Adipocytes & Pre—adipocytes:

Subcutaneous adipocytes and pre—adipocytes purified from surgical excess normal material. Protocols for culture and differentiation are provided.

Human Pancreatic Islets:

High quality, characterised human pancreatic islets are available for research use, including normal human islets and pancreatic islets from type 1 and type 2 diabetic donors.

All our human cells have been isolated from donors that are negative for HIV1/2, HCV and HBV infection and certified free of mycoplasmal, bacterial, yeast and fungal contamination. Please contact us for details of our current stock availability.

Cryopreserved human PBMCs and Cord Blood Mononuclear Cells are now available immediately, with functionality comparable to fresh isolations. We also offer highly purified monocyte, T-cells and B-cell subsets. These are available with basic or enhanced Characterization.

• PBMCs available from normal and diseased donors (includes autoimmune diseases: RA, SLE,
  Crohn’s, MS)
  
• Basic Characterization — includes age, gender, ethnicity, HLA-I data
  
• Enhanced Characterization — includes age, gender, ethnicity, HLA Class-I and Class-II data and ELISpot-IFNγ responses to PHA, CEF and CMV peptide pools
  

All cells are supplied with donor information including age, sex, liver pathology and medical history. We can also supply subcellular fractions: microsomes, S9 fractions (non—induced or induced and cytosols).

Cell and Tissue Culture Services

Human Tissue Services