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Tissue Sciences Facility

Omaha, Nebraska, US

The UNMC Tissue Sciences Facility has been established to provide basic and specialized histology, staining, immunohistochemistry, and imaging techniques to support the research community for studies on animal and human tissues. We offer a full range of cost effective, standardized services including tissue preparation, tissue procurement, processing, embedding, sectioning, standard and special stains for paraffin and frozen samples, single or multiplex immunohistochemistry/immunofluorescence, antibody optimization, digital bright field slide scanning, fluorescent whole slide imaging, tissue micro array construction, client-use instrumentation with technical support and image analysis services. Multiple software packages are available for image analysis, reconstruction, and deconvolution; these programs include Definiens, Metamorph, Velocity & ITEM Imaging Solutions.

We offer comprehensive pathology support for... Show more »

The UNMC Tissue Sciences Facility has been established to provide basic and specialized histology, staining, immunohistochemistry, and imaging techniques to support the research community for studies on animal and human tissues. We offer a full range of cost effective, standardized services including tissue preparation, tissue procurement, processing, embedding, sectioning, standard and special stains for paraffin and frozen samples, single or multiplex immunohistochemistry/immunofluorescence, antibody optimization, digital bright field slide scanning, fluorescent whole slide imaging, tissue micro array construction, client-use instrumentation with technical support and image analysis services. Multiple software packages are available for image analysis, reconstruction, and deconvolution; these programs include Definiens, Metamorph, Velocity & ITEM Imaging Solutions.

We offer comprehensive pathology support for basic science based on our extensive technical knowledge as well as through understanding the concerns of our research clients. Pathological consultation can be provided to researchers at all points in the research process. Training and mentoring is offered on specialized histology equipment, sample preparation and routine and custom histological procedures.

We offer a five business day turn-around for standard non-complex requests and a 10 business day turn-around for complex requests. Antibody optimization, tissue micro array construction and image analysis services are completed as fast as possible but are not held to a standard 5 or 10 business day turn-around.

Equipment and imaging programs utilized at the facility include:
Thermo-Fisher SlideMate, Slide Labeling
Sakura Tissue-Tek VIP5 Processor
Sakura Tissue-Tek TEC Embedding Station & Para trimmer & Wax Dispenser
Microtomes: Automated Thermo-Fisher HM355S, (2) Leica RM2255, Microm HM325
Cryostat: Leica CM 1850 Cryostat
Leica CryoJane: Leica CryoJane tape transfer system
Ventana Discovery Ultra, Automated IHC/ISH Research Slide Staining Platform
Leica Bond III, Automated IHC/ISH Clinical Platform
Sakura Tissue-Tek Prisma and Glas: Automated Slide Stainer & Cover Glass
Ventana iScan HT Bright Field High Throughput Slide Scanner
Leica DM750 Light Microscope & Leica ICC50 HD Digital Camera
Nikon e400 Microscope
Leica DM 5500B Immunofluorescence Microscope
Memamatsu Orca R2 C10600 and a Leica DFC 450 Digital Camera
Definiens Tissue Studio 3.6, Image Analysis Program
Velocity, 3D Image Analysis
Metamorph Image Analysis
ITEM SIS Soft Imaging Solutions

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Tissue Sciences Facility has not listed any services.

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Image Analysis
Starting at $66.50 per hour

Image analysis services is a custom service and pricing will vary based on the needs of the client; software systems aviable include Definiens, Metamorph, Velocity & SIS ITEM. Please request a quote for detailed pricing.

A consultation with laboratory personnel to review the project is required prior to receiving... Show more »

Image analysis services is a custom service and pricing will vary based on the needs of the client; software systems aviable include Definiens, Metamorph, Velocity & SIS ITEM. Please request a quote for detailed pricing.

A consultation with laboratory personnel to review the project is required prior to receiving materials for anaylysis. Pathologist consultation is available, please contact the laboratory for associated pricing. Materials are accepted into the laboratory Monday-Friday.

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Sample Packaging
Starting at $9.00 per unit

Slide Storage Box, 100 Slide Capacity, $9.00
Slide, Mailer, 5 Slide Capacity, $1.15
Slide, Folder, 20 Slide Capacity, $20.00

Slide Storage Box, 100 Slide Capacity, $9.00
Slide, Mailer, 5 Slide Capacity, $1.15
Slide, Folder, 20 Slide Capacity, $20.00

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Paraffin Sectioning
Starting at $3.75 per slide

Unstained Slide, $4.50
Additional Serial Section, $1.50
Level, $2.75
Unstained Slide, TMA, $7.00
Curl, $3.00
Curl with RNase Treatment, $4.85

Level steps must be indicated by a micron depth. Slides are sectioned at a routine 4 micron thickness unless specified by the client. A block ID list is requested for material... Show more »

Unstained Slide, $4.50
Additional Serial Section, $1.50
Level, $2.75
Unstained Slide, TMA, $7.00
Curl, $3.00
Curl with RNase Treatment, $4.85

Level steps must be indicated by a micron depth. Slides are sectioned at a routine 4 micron thickness unless specified by the client. A block ID list is requested for material verification and accurate slide labeling. Materials are accepted into the laboratory Monday-Friday.

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Tissue Microarrays
Starting at $300.00 per unit

TMA Construction Pricing is a custom service and pricing will vary based on the needs of the client; punch size options include 1-5mm. Please request a quote for detailed pricing.

A consultation with laboratory personnel to review the project is required prior to receiving materials for construction. Pathologist consultation... Show more »

TMA Construction Pricing is a custom service and pricing will vary based on the needs of the client; punch size options include 1-5mm. Please request a quote for detailed pricing.

A consultation with laboratory personnel to review the project is required prior to receiving materials for construction. Pathologist consultation is available, please contact the laboratory for associated pricing. Materials are accepted into the laboratory Monday-Friday.

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Immunofluorescence
Starting at $37.50 per slide

Single Antibody, IF Reaction, $37.50
Dual Antibody, IF Reaction, $60.00
Stain, DAPI Counterstain Only, $5.25
Coverslip Upgrade, Vectasheild Anti-Fade (hard-set), $6.75
Coverslip Upgrade, Vectasheild Anti-Fade w/DAPI (hard-set), $7.50

Slides are cover slipped with a routine aqueous mounting media, upgraded hard-set anti-fade... Show more »

Single Antibody, IF Reaction, $37.50
Dual Antibody, IF Reaction, $60.00
Stain, DAPI Counterstain Only, $5.25
Coverslip Upgrade, Vectasheild Anti-Fade (hard-set), $6.75
Coverslip Upgrade, Vectasheild Anti-Fade w/DAPI (hard-set), $7.50

Slides are cover slipped with a routine aqueous mounting media, upgraded hard-set anti-fade options are available. Please contact us for secondary antibody availability. We accept client provided primary and secondary antibodies for optimization services and staining. Materials are accepted into the laboratory Monday-Friday, please mark as light-sensitve. Client antibodies should be sent overnight on ice via FED EX for next day delivery.

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Antibody Optimization
Starting at $1.00 per sample

Antibody Optimization, Single DAB Reaction: Contact Facility
Antibody Optimization, Dual DAB/Purple Reaction: Contact Facility

A consultation is required to discuss the optimization. Many control tissues are available in the facility; if the facility does not have the necessary control, the client will be asked to provide... Show more »

Antibody Optimization, Single DAB Reaction: Contact Facility
Antibody Optimization, Dual DAB/Purple Reaction: Contact Facility

A consultation is required to discuss the optimization. Many control tissues are available in the facility; if the facility does not have the necessary control, the client will be asked to provide it. Antibody optimization services include 6 stained slides per antibody, additional slides may result in additional charges. Upgrade options are available and listed under IHC services.

Materials are accepted into the laboratory Monday-Friday.

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Cryoembedding
Starting at $7.00 per sample

Frozen, (OTC) Embed Only, $7.00

A specimen ID list is requested for material verification and accurate block labeling. Specimens are accepted into the laboratory Tuesday-Friday; materials should be sent overnight on dry ice via FED EX for next day delivery, except on Friday.

Frozen, (OTC) Embed Only, $7.00

A specimen ID list is requested for material verification and accurate block labeling. Specimens are accepted into the laboratory Tuesday-Friday; materials should be sent overnight on dry ice via FED EX for next day delivery, except on Friday.

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Cryosectioning
Starting at $6.00 per slide

Frozen, Unstained Slide, $6.00
Frozen, Serial Section, $4.50
Frozen, Curl, $6.00
Frozen, Level, $3.00

A block ID list is requested for material verification and accurate slide labeling. Slides are cut at a routine 10 micron thickness unless specified by the client. Frozen materials are accepted into the laboratory... Show more »

Frozen, Unstained Slide, $6.00
Frozen, Serial Section, $4.50
Frozen, Curl, $6.00
Frozen, Level, $3.00

A block ID list is requested for material verification and accurate slide labeling. Slides are cut at a routine 10 micron thickness unless specified by the client. Frozen materials are accepted into the laboratory Tuesday-Friday; materials should be sent overnight on dry ice via FED EX for next day delivery, except on Friday.

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Whole Slide Imaging
Starting at $11.25 per slide

Bright Field Slide Scan, Single Section, $11.25
Bright Field Slide Scan, Double Section, $12.25
Bright Field Slide Scan, Triple Section, $13.25
Bright Field Slide Scan, TMA, $14.25
Volume Scan, Add Level, $2.00
IF Slide Imaging-Contact Laboratory for Pricing

Volume scans include up to 3 focal planes at a one micron step. ... Show more »

Bright Field Slide Scan, Single Section, $11.25
Bright Field Slide Scan, Double Section, $12.25
Bright Field Slide Scan, Triple Section, $13.25
Bright Field Slide Scan, TMA, $14.25
Volume Scan, Add Level, $2.00
IF Slide Imaging-Contact Laboratory for Pricing

Volume scans include up to 3 focal planes at a one micron step.

A slide ID list is requested for material verification. Slides are accepted into the laboratory Monday-Friday; we request that external clients provide a flash drive for image transfer.

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VENTANA iScan Coreo
H&E Staining
Hematoxylin and eosin staining
Starting at $3.75 per slide

H&E Package, Paraffin, $17.25 (includes processing & embedding, slide w/one section & H&E stain)
H&E Stain Only, Paraffin, $3.75
H&E Stain Only, Frozen, $4.00
Counter Stain Only, Paraffin, $2.60

A material ID list is requested for material verification and accurate slide labeling. Slides are... Show more »

H&E Package, Paraffin, $17.25 (includes processing & embedding, slide w/one section & H&E stain)
H&E Stain Only, Paraffin, $3.75
H&E Stain Only, Frozen, $4.00
Counter Stain Only, Paraffin, $2.60

A material ID list is requested for material verification and accurate slide labeling. Slides are sectioned at a routine 4 micron thickness unless specified by the client. Materials are accepted into the laboratory Monday-Friday.

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Immunohistochemistry (IHC)
Starting at $37.50 per slide

Single Antibody, DAB Reaction, $37.50
Dual, Antibody, DAB/Purple Reaction, $75.00
Stain Upgrade, Single Purple Reaction, $6.90
Stain Upgrade, Single Yellow Reaction, $19.35
Stain Upgrade, Additional Amplification, $11.90

Please contact us for antibody availability and pricing; current offerings include 170 human and 10... Show more »

Single Antibody, DAB Reaction, $37.50
Dual, Antibody, DAB/Purple Reaction, $75.00
Stain Upgrade, Single Purple Reaction, $6.90
Stain Upgrade, Single Yellow Reaction, $19.35
Stain Upgrade, Additional Amplification, $11.90

Please contact us for antibody availability and pricing; current offerings include 170 human and 10 animal standardized tests. We accept client provided antibodies for optimization services and staining, detailed in optimization services. A slide or block ID list is requested for material verification and accurate slide labeling. Materials are accepted into the laboratory Monday-Friday. Client antibodies should be sent overnight on ice via FED EX for next day delivery.

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Paraffin Embedding
Starting at $8.50 per sample

Embedding, Paraffin, $8.50
Reprocess & Embed, Paraffin, $10.00
Additional Services, Bouins Fixative Alcohol Wash, $3.75
Additional Services, Regular Decal, $9.00
Additional Services, Rapid Decal, $9.00
Grossing Services, starting at $43.00 per hour

A specimen ID list is requested for material verification and accurate... Show more »

Embedding, Paraffin, $8.50
Reprocess & Embed, Paraffin, $10.00
Additional Services, Bouins Fixative Alcohol Wash, $3.75
Additional Services, Regular Decal, $9.00
Additional Services, Rapid Decal, $9.00
Grossing Services, starting at $43.00 per hour

A specimen ID list is requested for material verification and accurate block labeling. Specimens may be sent in 10% neutral buffered formalin or 70% ethyl alcohol. Consultation for proper specimen handling and fixation is available free of charge. Specialized processing protocols and consumables are available for specimens with specific needs, including processing sponges, paper, biopsy bag and cassettes. Materials are accepted into the laboratory Monday-Friday.

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Special Slide Staining
Starting at $7.00 per slide

Special Stain, PAS, $7.00
Special Stain, Masson Trichrome, Blue, $10.50
Special Stain, Alcian Blue 1.0 pH, $12.00
Special Stain, Alcian Blue 2.5 pH, $12.00
Special Stain, Oil Red O, $12.00
Special Stain, VVG, $17.25
Special Stain, Prussian Blue, $18.00
Special Stain, Toluidine Blue, $18.00
Special Stain, Gram,... Show more »

Special Stain, PAS, $7.00
Special Stain, Masson Trichrome, Blue, $10.50
Special Stain, Alcian Blue 1.0 pH, $12.00
Special Stain, Alcian Blue 2.5 pH, $12.00
Special Stain, Oil Red O, $12.00
Special Stain, VVG, $17.25
Special Stain, Prussian Blue, $18.00
Special Stain, Toluidine Blue, $18.00
Special Stain, Gram, $18.00
Special Stain, Fast Red, $18.00
Special Stain, Giemsa, $18.00
Special Stain, Acid-Fast Bacilli, $18.00
Special Stain, FITE Acid Fast, $18.00
Special Stain, Congo Red, $18.00
Special Stain, Colloidal Iron, $18.00
Special Stain, Mucicarmine, $18.00
Special Stain, Luxol Fast Blue, $18.00
Special Stain, Cresyl Violet, $18.00
Special Stain, Sirius Red, $18.00
Special Stain, Alizan Red, $18.00
Special Stain, Von Kossa, $18.00
Special Stain, Jones Silver, $18.00
Special Stain, Grocott's Methenamine Silver, $18.00
Special Stain, Steiner Stain, $18.00
Special Stain, Reticulin, $18.00
Special Stain, Fontana-Masson, $18.00
Special Stain, Movat Pentachrome, $18.00
Special Stain, Auramine-Rhodamine, $18.00
Special Stain, Alcian Blue-PAS, $18.00
Special Stain, PAS-Fungus, $18.00
Special Stain, PAS-Digest, $18.00

Materials are accepted into the laboratory Monday-Friday.

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Data Services
Price on request
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Microscopy
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Protein Expression Visualization
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Clinical Research
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Computational Modeling
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Protein Services
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Biology
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Immunostaining
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Cells and Tissues
Price on request
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Biospecimens
Price on request
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Histological Staining
Price on request
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Common Histochemical Stains
Price on request
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Tissue Embedding
Price on request
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Tissue Sectioning
Price on request
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Imaging & Spectroscopy
Price on request
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Tissue Processing
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Product Development, Testing, and Packaging
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Clinical Laboratory Services
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Clinical and Anatomic Pathology
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Antibody Services
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Packaging Services
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Biochemistry & Molecular Biology
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July 10, 2016

Positive review received for Immunohistochemistry (IHC):

"Fast and skilled service, will be glad to use them again."

August 21, 2013

Positive review received for Whole Slide Imaging:

"Project was completed as agreed upon in a timely manner. David Wert was very responsive to communication."

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