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Tissue Culture Facility

Chapel Hill, North Carolina, US

The TCF currently occupies approximately 5000 net square feet (nsf) of laboratory space, not including warm room, cold room, or the specialized microscope space, that consists of six rooms that accommodate media preparation, cell culture manipulations, testing procedures, B-cell immortalization services, or other activities that must be functionally separated. A walk-in incubator and walk-in cold storage room are both dedicated to TCF functions. A main administrative area serves as the pick-up area for users to obtain research materials, place orders, or consult with TCF staff.

The facility has seven biosafety cabinets, twelve double-chamber CO2 incubators, two automated cell counters, four specialized cell bioreactors, large and small volume centrifuges, and microscopes to accommodate a wide range of cell culture activities. All microscopes in the TCF’s program have high-resolution digital cameras and digital... Show more »

The TCF currently occupies approximately 5000 net square feet (nsf) of laboratory space, not including warm room, cold room, or the specialized microscope space, that consists of six rooms that accommodate media preparation, cell culture manipulations, testing procedures, B-cell immortalization services, or other activities that must be functionally separated. A walk-in incubator and walk-in cold storage room are both dedicated to TCF functions. A main administrative area serves as the pick-up area for users to obtain research materials, place orders, or consult with TCF staff.

The facility has seven biosafety cabinets, twelve double-chamber CO2 incubators, two automated cell counters, four specialized cell bioreactors, large and small volume centrifuges, and microscopes to accommodate a wide range of cell culture activities. All microscopes in the TCF’s program have high-resolution digital cameras and digital image capture capabilities. The facility has temperature-critical storage space for samples or materials as follows; a +37°C walk-in warm room, a +4°C walk-in cold room, eight –20°C upright freezers, and three –80°C upright freezers. The TCF has nine liquid nitrogen storage freezers that accommodate the facility’s general cell repository, long-term liquid nitrogen storage for Cancer Center members, primary and back-up storage for immortalized human lymphoblast cultures generated from clinical based research protocols and other human genetics repository functions. There is also a programmable-rate freezer for the automated cryopreservation of cell cultures for the TCF repository cells and for users’ cultures.

The TCF is well equipped and well staffed to meet the current and future specialized research support requirements of the UNC Lineberger Comprehensive Cancer Center members, the UNC research community in general, and other area research-oriented institutions

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Tissue Culture Facility has not listed any services.

Mammalian Cell Culture
Price on request
  • anchorage cell systems
  • bioreactor services
  • B-lymphocyte immortalization
  • cell cryopreservation
  • cell line acquisition
  • cell repository
  • hollow-fiber cell culture system
  • large & small scale cell cultures
  • liquid nitrogen storage
  • monoclonal antibody isotyping
  • monoclonal antibody production
  • mycoplasma treatment/cure
  • suspension cell systems
  • anchorage cell systems
  • bioreactor services
  • B-lymphocyte immortalization
  • cell cryopreservation
  • cell line acquisition
  • cell repository
  • hollow-fiber cell culture system
  • large & small scale cell cultures
  • liquid nitrogen storage
  • monoclonal antibody isotyping
  • monoclonal antibody production
  • mycoplasma treatment/cure
  • suspension cell systems
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Monoclonal Antibody (mAb) Production
Starting at $65.00 per sample

The UNC Institutional Animal Care and Use Committee (IACUC) has informed us that new guidelines will result in greater restrictions on the use of animals for the production of monoclonal antibodies in ascites. As an alternative, the Tissue Culture Facility has expanded and improved techniques for in vitro monoclonal antibody... Show more »

The UNC Institutional Animal Care and Use Committee (IACUC) has informed us that new guidelines will result in greater restrictions on the use of animals for the production of monoclonal antibodies in ascites. As an alternative, the Tissue Culture Facility has expanded and improved techniques for in vitro monoclonal antibody production using your hybridoma. The TCF does not currently create hybridomas, but can arrange to have that service provided for a fee at the NCSU Hybridoma Facility. The Tissue Culture Facility (TCF) can provide the following services related to monoclonal antibody in vitro production using existing hybridomas.


I. Standard production using your hybridoma in a fetal bovine serum (FBS) supplemented medium.

This is the most basic production system whereby your hybridoma is grown in FBS supplemented cell culture medium to the volume requested. The maximum cell density possible is attained, the cells are allowed to produce antibody in the medium until approximately 80% of the cell population becomes non-viable, and the medium containing the antibody is collected. Cells are typically grown in standard spinner cultures for this technique.

Volumes available using this system:
1 to 75 liters

Typical Mab concentration:
10 to 50 ug/ml

Typical delivery time (start from frozen vial):
5 liters; 3 to 4 weeks

Cost:
$65 per liter (non-members)


II. Standard production using your hybridoma in a serum-free cell culture medium.

Production is as above except the hybridoma cells are weaned to serum-free medium prior to production. This may be important to investigators concerned with subsequent purification as the absence of extraneous serum proteins makes purification easier. Time is added (two to three weeks) to the delivery time due to the weaning process.

Volumes available using this system:
1 to 75 liters

Typical Mab concentration:
10 to 50 ug/ml

Typical delivery time (start from frozen vial):
5 liters; 3 to 5 weeks

Cost:
$65 per liter + $25 per week for weaning (~2 weeks)


III. Production using Hollow-fiber Bioreactor / Special membrane design Integra flasks.

This system is recommended for investigators requiring relatively large amounts of antibody at medium to high concentrations. Your hybridoma is weaned to serum-free medium and loaded into a hollow-fiber bioreactor or a new stationary system by Integra. Cells are grown in the extra-capillary space of the cartridge or in a special membrane area in the Integra flasks to very high densities. Typically, seven milliliters of medium containing the antibody is collected every two to three days over a time period necessary to obtain the total required amount of antibody. The antibody isotype is monitored during weaning and production and is included in the cost.

Volumes available using this system:
limited only by time/cost

Typical Mab concentration:
0.5 to 3 mg/ml

Typical delivery time (start from frozen vial):
50 ml; 4 to 8 weeks

Cost:
$600 flat fee for 50 ml; + $25 per 7 ml after that


IV. Isotype Determination.

We can determine and monitor the isotype of your monoclonal antibody during production if required. (This service is included free in the hollow-fiber production package.)

Isotype determination cost: $15.00 per sample

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Biology
Price on request
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Cells and Tissues
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Biochemistry & Molecular Biology
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Protein Services
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Antibody Services
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