Timur Yarovinsky, MD, PhD

Kinetic and endpoint assay for extracellular ATP release from cultured cells with the sensitivity at low nM range.
Many cells release their ATP into extracellular space in response to various triggers, such as microbial toxins, mechanical stimulation, apoptosis, etc. Extracellular ATP (eATP) and its metabolites have profound effects on migration and activation of leukocytes, inflammation, and epithelial and endothelial barriers during infection. Using a luminescence-based assay we can measure eATP in real time for kinetic studies or at predetermined time points. We have shown eATP release from human lung epithelial cells after exposure to a pore-forming toxin from S. aureus (Lizak & Yarovinsky, Cell Host Microbe. 2012 Jan 19; 11(1): 70–80).

• Staphylococcal infection mouse model (pneumonia, sepsis) using laboratory and clinical isolates; price on request.

• Endotoxemia or sterile inflammation; price on request.

• Liver acute phase response; price on request.

Prices from $20.00 per sample.

Using luminescent or fluorescent-based assay, we can measure viability of suspension or adherent cultured cells at different time points after treatment.

T cell proliferation (CFSE) in vitro. Prices from $15 per sample.

Flow cytometry analyses (surface and intracellular) and phenotyping of T cells; prices from $45 per sample.

Prices from $15.00 per sample.

Prices from $15.00 per sample.

From $5.00 per well.

Research project management (experimental design, response to reviews, progress reports); from $80 per hour.

Confidential review and editing of manuscripts, dissertations and grant applications; from $80 per hour.

Animal protocol writing, editing, submission to IACUC, review; from $80 per hour.

We will gladly evaluate novel research reagents and software. We will provide written report with specific comments on pros and cons along with suggestions for improvements.We have previously provided beta testing for LI-COR, Promega, and Corning.