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TIGM Transgenic Core

College Station, Texas, US

The Texas A&M Institute for Genomic Medicine (TIGM) is an essential resource for any researcher looking to obtain knockout mice and embryonic stem (ES) cells quickly and with favorable intellectual property (IP) terms. Our resources include the world's largest gene trap library of ES cells in the C57BL/6N mouse strain and access to the largest library of ES cells in the 129/SvEvBrd mouse strain. TIGM provides both ES cell clones and mice to the public and private international research community. This facility is AALAS certified.

TIGM Transgenic Core has not listed any services.

Blastocyst Injection
Starting at $2,300.00 per sample

Investigator provides two vials of the frozen clone. The service includes injection of at least 30 blastocysts/clone. All resulting offspring will be transferred to the investigator at weaning.


Turn-around time: 7-8 weeks


*This price is for academic and non-profit customer.

Investigator provides two vials of the frozen clone. The service includes injection of at least 30 blastocysts/clone. All resulting offspring will be transferred to the investigator at weaning.


Turn-around time: 7-8 weeks


*This price is for academic and non-profit customer.

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Cryopreservation
Starting at $100.00 per sample

Cryostorage (2 year fee) $100
The samples will be stored in MVE Model 815-190F liquid nitrogen vapor freezers

Cryostorage (2 year fee) $100
The samples will be stored in MVE Model 815-190F liquid nitrogen vapor freezers

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Embryonic Stem Cell (ESC) Culture
Starting at $225.00 per sample

Expansion of targeted ES cell clones: $225 per clone

Expansion of targeted ES cell clones: $225 per clone

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Embryo Transfer
Starting at $410.00 per sample

Frozen Embryo transfer $410
Investigator provides at least 4 straws each containing approximately 20 embryos. 2 straws will be used for transfer. The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning.

Live Embryo... Show more »

Frozen Embryo transfer $410
Investigator provides at least 4 straws each containing approximately 20 embryos. 2 straws will be used for transfer. The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning.

Live Embryo transfer $410
Investigator provides early stage embryos in M2 medium or alternatively freshly isolated uterine or oviduct tissues from timed pregnancies for the core to flush. The investigator must coordinate with the core at least a week in advance so that the core can set up recipient females at the appropriate stage. All resulting offspring will be transferred to the investigator at weaning.

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Embryonic Stem Cell Electroporation
Starting at $3,400.00 per sample

Electroporation service package $3,400
The service includes linearization/purification/ electroporation of targeting construct; isolation/expansion of drug-resistant colonies in 96-well format (at least 2 plates); preparation of cell pellet plates in 96-well format and expansion of all targeted ES cell clones as specified by the... Show more »

Electroporation service package $3,400
The service includes linearization/purification/ electroporation of targeting construct; isolation/expansion of drug-resistant colonies in 96-well format (at least 2 plates); preparation of cell pellet plates in 96-well format and expansion of all targeted ES cell clones as specified by the customer.

Additional services
Identification of targeted ES cell clones by PCR: $1,100 per plate
Expansion of targeted ES cell clones: $225 per clone

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Embryonic Stem Cell Lines
Price on request

C57Bl/6 Mice and ES Cell Clones
TIGM operates a gene trap library - a premier knockout mouse ES cell resource - that contains over 350,000 cell lines in the C57BL/6 mouse background. This library contains mutated ES cell clones representing more than 10,000 genes.

129/SvEvBrd Mice and ES Cell Clones
TIGM has limited access to... Show more »

C57Bl/6 Mice and ES Cell Clones
TIGM operates a gene trap library - a premier knockout mouse ES cell resource - that contains over 350,000 cell lines in the C57BL/6 mouse background. This library contains mutated ES cell clones representing more than 10,000 genes.

129/SvEvBrd Mice and ES Cell Clones
TIGM has limited access to a privately held 129/SvEvBrd gene trap library. This gene trap library contains more than 270,000 sequence-tagged embryonic stem cell clones in the 129/SvEvBrd mouse strain representing mutations in over 10,000 genes.

ES cell clone orders
Quality control procedures for ES cell clones:
qPCR for Neo to ensure one copy of gene trapping vector is present in the genome
qPCR for SRY to ensure there is no loss or degradation of the Y chromosome
iPCR to ensure the gene trapping vector is in the correct location and orientation
Assays to ensure cultures are free of mycoplasma, fungi and bacteria contamination

For standard ES cell clones orders, TIGM will supply:
Up to three clones per gene confirmed to carry the correct mutations.
Two vials per clone, each containing approximately 5.0x106 ES cells.
QC report with validated genomic insertion site and recommended genotyping strategies

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Embryonic Stem Cell Screening
Starting at $1,100.00 per sample

Identification of targeted ES cell clones by PCR: $ 1,100 per plate

Identification of targeted ES cell clones by PCR: $ 1,100 per plate

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Knockout Mouse Development
Price on request

Knockout Mice Orders
QC for the ES cells before microinjection
- qPCR for Neo to ensure one copy of gene trapping vector is present in the genome
- qPCR for SRY to ensure there is no loss or degradation of the Y chromosome
- iPCR to ensure the gene trapping vector is in the correct location and orientation
- Assays to ensure... Show more »

Knockout Mice Orders
QC for the ES cells before microinjection
- qPCR for Neo to ensure one copy of gene trapping vector is present in the genome
- qPCR for SRY to ensure there is no loss or degradation of the Y chromosome
- iPCR to ensure the gene trapping vector is in the correct location and orientation
- Assays to ensure cultures are free of mycoplasma, fungi and bacteria contamination
Tail samples are genotyped with mutant strategies specific for the insertion site before the animals are shipped. A recommended strategy to genotype for the wild type allele is also provided.

For standard knockout mouse orders, TIGM will supply:
Four (4) mutant mice, heterozygous for the selected mutation, including at least one (1) breeding pair
Validated genotyping protocols
Animal health data

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Animal Embryo and Sperm Cryopreservation
Starting at $1,400.00 per sample

Investigator transfers 6-8 practiced stud males, 3-6 months of age, to TIGM quarantine. The core will perform two sessions and will cryopreserve at least 200 embryos at the morula stage. Additional sessions can be performed at a cost of $650. The cryopreservation fee includes embryo viability testing. After the first year there is... Show more »

Investigator transfers 6-8 practiced stud males, 3-6 months of age, to TIGM quarantine. The core will perform two sessions and will cryopreserve at least 200 embryos at the morula stage. Additional sessions can be performed at a cost of $650. The cryopreservation fee includes embryo viability testing. After the first year there is a biannual $100 liquid nitrogen storage fee.

Sperm Cryopreservation $500
Sperm from two male mice will be cryopreserved using the protocol from the Jackson Laboratory. The cryopreservation fee includes sperm viability testing by in vitro fertilization. After the first year there is a biannual $100 liquid nitrogen storage fee.

Sperm Cryopreservation (Jax Kit) $1,500
Sperm from two male mice will be cryopreserved using the kit from the Jackson Laboratory. The viability of the frozen sperm will be tested at the Jackson Laboratory by in vitro fertilization and stored at the Jackson Laboratory. After 3 years there is a $170 storage fee per strain per year.

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Rodent Rederivation
Starting at $1,100.00 per sample

Rederivation via embryo transfer $1,100
Investigator transfers 3-6 practiced stud males, 3-6 months of age, to TIGM quarantine for mating with superovulated females. The core will perform 1-2 sessions and will transfer at least 100 embryos at the morula stage.

Rederivation via IVF (TIGM or Jax sperm... Show more »

Rederivation via embryo transfer $1,100
Investigator transfers 3-6 practiced stud males, 3-6 months of age, to TIGM quarantine for mating with superovulated females. The core will perform 1-2 sessions and will transfer at least 100 embryos at the morula stage.

Rederivation via IVF (TIGM or Jax sperm samples) $1,100
Investigator provides at least 2 straws each containing at least 40-50 ul of frozen sperm. The core will isolate oocytes from at least 5 superovulated females, perform IVF and transfer the resulting embryos to recipient females. All resulting offspring will be transferred to the investigator at weaning.

Rederivation via IVF (Other Samples) $3,200
Investigator provides at least 4 straws each containing approximately 200 ul of frozen sperm. The core will perform two sessions and will use oocytes from 20 donors per vial of sperm. The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning.

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Mouse
Pronuclear Injection
Starting at $2,600.00 per sample

Pronuclear Injection $2,600
Investigator provides at least 20 ug of plasmid DNA at a concentration of 500 ug/ml. Includes injection of at least 125 FVB embryos. All resulting offspring will be transferred to the investigator at weaning.

Pronuclear Injection $2,600
Investigator provides at least 20 ug of plasmid DNA at a concentration of 500 ug/ml. Includes injection of at least 125 FVB embryos. All resulting offspring will be transferred to the investigator at weaning.

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Genetically Engineered Mice
Price on request
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Genetic Engineering
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Biology
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Mammalian Cell Culture
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Transgenic Mouse Services
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Biospecimens
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Animal Model Housing and Maintenance
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Cryostorage and Cryoprocessing
Price on request
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Animal Biospecimens
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Animal Models and Studies
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Transgenic Animal Model Development
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Cells and Tissues
Price on request
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Biospecimen Management and Storage
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Cell Lines
Price on request
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2018-05-12 09:12:17 -0700

Net Promoter Score of 10 received for Animal Embryo and Sperm Cryopreservation.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

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