The Amnion Foundation is a registered 501(c)3 organization that provides custom cell isolation and assay services from human birth tissue (placenta, umbilical cord, and derived cells). We offer placental stem cells that are derived from term placenta after the birth. These cells exhibit multi-lineage plasticity and are of baby origin. We also offer umbilical vein endothelial cells (hUVEC) and other custom cells and services. We will provide cells that have been isolated and maintained at physiologic oxygen levels as well as cells isolated and maintained under typical laboratory conditions (21% Oxygen). We provide biological sample sterility testing for research samples, mycoplasma testing for research samples, phenotypic profiling of cells by flow cytometery, extracellular matrix preparation from birth tissue, and collection of conditioned media and/or microvesicle preparations from tissue or isolated cells.
We provide BACT/Alert 3D testing to assess sterility of biological samples as a fee-for-service. We also provide Mycoplasma testing through real-time PCR w/7500 FAST system using MYCOSeq. This testing service is ideal for testing of research samples intended for commercial distribution (isolated cells, conditioned media, or extracellar matrix samples) or sterility testing of samples prior to preclinical research studies.
Birth tissues are a plentiful and pristine source of living cells that can support a broad spectrum of experimental model systems and therapeutic approaches. Every placenta is comprised of both maternal-origin and baby-origin cells, which creates a unique opportunity to source cells without any risk to the mother or child. At the Amnion Foundation, we specialize in isolating and banking human cells for use by our partners and clients in the pursuit of new therapies.
HUMAN PLACENTA-DERIVED STEM CELLS (HPSC)
Isolated from the human placenta after a healthy birth, baby-origin hPSCs are phenotypically stable and can be expanded extensively to create large banks of cells to support research, pre-clinical, and clinical programs. The potential applications for hPSCs are supported by over two decades of peer-reviewed scientific publications and the advancement of programs at highly accredited research institutes, including the Wake Forest Institute of Regenerative Medicine.
BIRTH TISSUE CELLS & SERVICES
The placenta and umbilical cord tissue contain multiple highly valuable cell types, including cytotrophoblasts, a variety of endothelial cells, and specialized immune cells (Hofbauer cells) that can support the establishment of model systems aimed at assessing placental barrier function, vasculogenesis, and infectious diseases. Furthermore, many of these cell types (endothelial cells, pericytes, and variety of stem and progenitor cells) have tremendous potential as components of regenerative medicine products. The Amnion Foundation is committed to providing quality cells and isolation services for our partners and clients.
We provide custom isolation of specialized fetal macrophages -- Hofbauer Cells -- from human placenta. Please contact us to determine the ideal number and format to fit your research needs.
We will conduct cell-based assays using placental stem cells, endothelial cells, or other birth tissue-derived cell types, to your specification and provide raw data, processed data, and a summary report of outcomes. Our standard assays include basic dose-response using MTT or MTS readout, ELISAS, cell migration, and tube formation.
We will work with our clients to develop cell-based assays for their endpoints using our available primary cell inventory. Our capabilities include basic biochemistry assays (metabolism, viability, enzymatic activity), secreted molecular measurement (ELISA), gene expression, cell migration, and tube formation.
We provide cryopreserved inventory of well-characterized placental stem cells and human umbilical vein endothelial cells (HUVEC), isolated and maintained at either atmospheric (21%) or physiologic oxygen tension. We also offer custom isolation / formats for cytotrophoblasts, villous capillary endothelial cells, and Hofbauer cells. We can provide fresh or dehydrated amnion and/or chorion, or other birth tissue components to support research activities.
We provide assistance in the design and protocol development of assays involving the use of placental stem cells, endothelial cells, macrophages, and other primary human cell types. We can conduct wet-lab optimization of assays using standard analytical equipment (fluorescence imaging, multi-function plate readers with temperature and oxygen control, qRT-PCR). We have extensive experience in primary cell culture and handling and can adapt assays to challenging cell types.
We provide human placental stem cells, isolated from term placenta after birth. These cells are derived from the fetal side of the placenta and are thus baby-origin (vs. maternal origin). They have multi-lineage potential and are being advanced for regenerative medicine applications.
We also offer hUVEC, cytotrophoblasts, Hofbauer cells, and other birth tissue-derived cells.
We conduct cell isolations to client specification from birth tissue. We will also conduct protocol optimization for clients as a fee-for-service.
We have access to human birth tissue (placenta and umbilical cord) from within the United States. All of the tissues we receive are collected through licensed and established tissue collection agencies with all protocols and approvals in place, in compliance with all local, state, and federal regulations governing the use of human tissue specimens. We can provide fresh tissue samples, formalin-fixed paraffin-embedded tissue samples, or isolated cells or derivatives. In many cases, we can provide cells suitable for assay use that are proactively consented for use in research that includes genetic testing. All of our specimens are consented for use in research by not-for-profit and for-profit entities, can be transferred to third parties, and can be used in commercial activities.
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