SynVivo® is a physiological, cell-based microfluidic platform that provides a biologically realistic microenvironment allowing real-time study of cellular behavior, drug delivery and drug discovery. SynVivo 3D tissue and organ-on-chip models recreate complex in vivo microenvironments including scale, morphology, hemodynamics, and cellular interactions with a side-by-side architecture enabling real-time visualization and analysis. SynVivo's 3D tissue models have been extensively validated in oncology, neuroscience, inflammation and toxicology applications. Models include SynTumor (Cancer), SynBBB (blood brain barrier), SynRAM (Inflammation) and SynTox (Toxicology). Services include target validation, compound screening, biomarker analysis, ADME/Tox and mechanism of action studies. Assay Development services can be performed to develop new tissue/organ models, assays or custom chip designs. See http://www.synvivobio.com Unlike well plate testing performed under static conditions, SynVivo reproduces the realistic dynamic conditions for analyzing cell-drug and cell-cell interactions thereby providing an accurate in vitro platform to study and elucidate the mechanisms of success and failure. Compared to in vivo animal studies, SynVivo allows real-time visualization and analysis of the assay in a controlled environment.
SynRAM 3D tissue model www.synvivobio.com/Synram allows the study of the entire inflammation pathway in a realistic and dynamic environment. SynRAM delivers a physiologically realistic model and enables real-time tracking of rolling, adhesion and migration processes by mimicking a histological slice of co-cultured tissue and/or tumor cells with a lumen of endothelial cells. SynRAM has been successfully validated against in vivo studies showing excellent correlation with rolling velocities, adhesion patterns and migratory processes.
This model can be used for studies of:
Real-time, quantitative immune-endothelial interactions
Cell rolling, adhesion and migration
Screening for cell surface biomarkers
Screening for activators/inhibitors of inflammation
(1) A novel microfluidic assay reveals a key role for protein kinase C δ in regulating human neutrophil-endothelium interaction. Soroush F, Zhang T, King DJ, Tang Y, Deosarkar S, Prabhakarpandian B, Kilpatrick LE, Kiani MF. J Leukoc Biol November 2016 100:1027–1035.
(2) Bioinspired Microfluidic Assay for In Vitro Modeling of Leukocyte–Endothelium Interactions. G. Lamberti, B. Prabhakarpandian, C. Garson, A. Smith, K. Pant, B. Wang, and M.F. Kiani. Anal. Chem., 2014, 86 (16), pp 8344–8351.doi:10.1021/ac5018716
SynBBB 3D blood brain barrier model from SynVivo recreates the in vivo microenvironment by mimicking a histological slice of brain tissue cells in communication with endothelial cells across the blood brain barrier (BBB). See http://www.synvivobio.com/synbbb. Shear-induced endothelial cell tight junctions, which cannot be achieved in the Transwell® model, are easily achieved in the SynBBB model using physiological fluid flow. Formation of tight changes can be measured using biochemical or electrical analysis (assessing changes in electrical resistance) with the SynVivo Cell Impedance Analyzer. Interactions between brain tissue cells and endothelial cells are readily visualized in the SynBBB assay. Transwell models do not allow real-time visualization of these cellular interactions, which are critical for understanding of the BBB microenvironment.
The SynBBB model can be used for the following studies:
•Drug permeability: Evaluate real-time permeability of therapeutics and small molecules across the endothelium of the BBB. Readouts can be biochemical or electrical analysis (assessing changes in electrical resistance)
•Inflammation: Understand the underlying mechanisms of inflammatory responses on the regulation of the BBB.
•Cell migration: Visualize and quantify in real-time migration of immune cells across the BBB.
•Tight junction proteins: Determine the levels of tight junction proteins namely zonula occludens, claudins and occludins which regulate the BBB.
•Transporter proteins: Analyze functionality of transporter proteins (e.g. Pgp) in normal and dysfunctional BBB.
•Omic changes: Perform genomic, proteomic and metabolic analysis on normal and dysfunctional BBB.
•Neurotoxicity: Analyze toxicity effects of chemical, biological and physical agents on the cells of the BBB.
•Neuro-oncology: Investigate effects of the tumor cells on the BBB.
Human and Rat BBB model can be used for the studies indicated using primary cells or cell lines.
Recent Publications using the SynBBB model
(1) Trastuzumab Distribution in an In-Vivo and In-Vitro Model of Brain Metastases of Breast Cancer Tori B. Terrell-Hall, Mohamed Ismail Nounou, Fatema El-Amrawy, Jessica I.G. Griffith and Paul R. Lockman Oncotarget. 2017; 8:83734-83744
(2) Permeability across a novel microfluidic blood tumor barrier model. Tori B. Terrell Hall, Amanda G. Ammer, Jessica I. G. Griffith and Paul R. Lockman Fluids and Barriers of the CNS (2017) 14:3
(3) A Novel Dynamic Neonatal Blood-Brain Barrier on a Chip. S. Deosarkar, B. Prabhakarpandian, B. Wang, J.B. Sheffield, B. Krynska, M. Kiani. PLOS ONE, 2015
(4) SyM-BBB: A Microfluidic Blood Brain Barrier Model B. Prabhakarpandian, M.-C. Shen, J.B. Nichols, I.R. Mills, M.S.-Wegrzynowicz, M. Aschner, K. Pant, Lab on a Chip, 2013, 13, 1093-1101
Using our co-culture technologies we are able to screen your most promising drug candidates for toxicity affects before you have to resort to costly and time consuming in vivo trials.
Many of the most promising drugs discovered today rely not just on bio-chemical interactions but specific cell-cell interactions that can only be simulated with realistic morphology and physiology.
You’ve found a drug that works well in static well plate tests. We can assess that drug’s performance under more physiological realistic conditions using the SynVivo technology.
SynVivo® is a physiological, cell-based microfluidic platform that provides a biologically realistic microenvironment allowing real-time study of cellular behavior, drug delivery and drug discovery. SynVivo 3D tissue models recreate complex in vivo microenvironments including scale, morphology, hemodynamics, and cellular interactions with a side-by-side architecture enabling real-time visualization and analysis. SynVivo's 3D tissue models have been extensively validated in oncology, neuroscience, inflammation and toxicology applications. Services include target validation, compound screening, biomarker analysis, ADME/Tox and mechanism of action studies. Assay Development services can be performed to develop new tissue/organ models, assays or custom chip designs. See http://www.synvivobio.com
ADME and DMPK Studies Services
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