Sunomix Biosciences is a leading Contract Research Organization based in JLABS. Johnson & Johnson Innovation, JLABS San Diego, California, that manages a platform for outsourcing Products and Services across all areas of life sciences. We have many platform for genome sequencing, RNA Sequencing, RT-PCR, Genotyping, Protein/antibodies expression & purification, microarrays services, Multiplex beads arrays, Pre-clinical and clinical studies, Transgenic Mouse production and many other Molecular, Cellular, Biochemistry & Immunology services under GLP and non GLP. We offers competitive and high quality services to domestic and international pharmaceutical, biotech, academic, medical device and related industries.
The laboratory facilities for Sunomix Biosciences, Inc, located into JLABS. Johnson & Johnson Innovation, JLABS, established by Janssen Research & Development in La Jolla, CA in January 2012, provides innovative companies with an efficient way to discover and develop solutions to today’s healthcare challenges. This 40,000 sqft building located at 3210 Merryfield Row, 92121, gives companies facilities containing over 70 pieces of large equipments including core biology and chemistry facilities, three tissue culture rooms, two cold rooms, conference/meeting rooms and a business center. Our individual lab units have modern amenities for research and come equipped with features such as HPLC, LCMS, Flow Cytometer, Fluorescent Microscope, NMR Spectrometer, Microarray.....Work is done under GLP/GMP if needed.
Anti-proliferative IC50 will be determined using Promega's Cell Titer Glo reagent. An 8 point curve will be generated with duplicate values at each point. Experiment will be repeated and an average of two IC50 values will be reported. One compound + one control compound will be tested in three cell lines.
Antigen Expression and Purification
Protein Expression and Purification
• Bacterial, Insect and Mammalian Systems
Enzyme-linked immunosorbent assay
Kit-based custom designed ELISA development and services
Indirect, Sandwich, competitive
Homogenization/Sonication of cell/tissue lysates
Transfection/Transduction of mammalian/non-mammalian cells
Optimization of protocols for commercial kits
Analyze serum, plasma, cells, tissues
Analyze chromogenic substrates
Multi-channel and automatic pipets
Number of samples processed in a single experiment: 96-well plate format
Typical turn-around time: 7 days
ELISA with TMB substrate. Primary Antibody, standards.
Purified proteins/cell lysate/ tissue lysate samples will be run on SDS-polyacrylamide gels followed by transfer of the fractionated proteins/polypeptides/fragments onto PVDF membrane. The membrane is probed with a primary-secondary antibody pair via chemiluminescent method to detect the protein/fragment of interest. This service needs to be optimized on a case basis to suit the optimal fractionation of protein samples provided by the customer.
Time Line: 5-10 Days.
Summary of Service: Western Blot Run (WB) will be optimized to fractionate the purified proteins/cell lysate/ tissue lysate samples on SDS-polyacrylamide gels followed by transfer of the fractionated proteins/polypeptides/fragments onto PVDF or nitrocellulose membrane. The membrane is probed with a primary-secondary antibody pair via chemiluminescent method to detect the protein/fragment of interest. If STD proteins are not transferred, we will repeat the electrophoresis, blotting and probing steps to obtain a satisfactory chemiluminescent signal. This service often needs to be optimized on a case basis to suit the optimal fractionation of protein samples provided by the customer.
Number of samples per run: 1-8
WB System: (1) Reducing or Non-Reducing SDS-gel (2) Linear Gradient (4-20% SDS-PAGE Gel) or Fixed conc. (4, 10, 12% or custom SDS-PAGE Gel) (3) TRIS-Glycine buffer system or any other system preferred by customer (4) Choice of marker/standard proteins (5) Wet-transfer of proteins on PVDF membrane (6) Probing of WB membrane with one primary - secondary antibody pair and visualization by chemiluminescent method.
Deliverables: Digital image of the blot via email.
Requirements: Customer provides the following
(1) Pure Protein samples in Sample Buffer (>50ug/protein/run; Conc. = >1mg/ml) or Cell/tissue lysates in Sample Buffer (>500ug/sample/run; Conc. = >1mg/ml). Alternatively, we may attempt to solublize the samples in Sample buffer for a nominal fee ($10/pure protein sample).
(2) Primary-secondary antibody pair to detect the protein/fragment of interest. Alternatively, customer may purchase the antibodies available from us.
Available cell lines and tissues:
HeLa, MCF-7, A549, PC3, SK-N-SH, NIH/3T3,
LNCaP, SuDHL-16, CAPAN-1, Mosquito cell line
Mouse, Rat and Human organs/tissues.
Generation of Stable Cell Line in 28 Days
Stable cell lines over-expressing a protein of interest (or shRNA construct targeting a gene of interest) are important research tools, particularly for the production of recombinant antibodies and other proteins, the study of gene functions, drug screening, and other applications. In addition, utilizing a stable mammalian cell line can make products more acceptable for clinical use. Stable cell lines will reliably grow for extended periods of time and can continue to express a transgene at a consistent rate. However, production of stable cancer cell lines and primary cells can be very expensive, labor-intensive, and time-consuming.
Sunomix Biosciences offers several stable cell line generation services: protein overexpressing cell lines, RNAi knockdown cell lines, and reporter stable cell lines (luciferase, GFP, RFP, YFP). Company scientists provide expert services with years of cell culture experience with more than 150 cancer cell lines (available in house). All clonal stable cell lines are developed based on individual client specifications, using highly efficient gene delivery technologies to produce stably expressing clonal cell lines in just 28 days. We can produce multiple clonal cell lines with various expression levels (low / medium / high expression).
Typically, antibiotic resistance or fluorescent reporter gene markers are incorporated into the plasmid DNA construct to facilitate selection process. These selection markers can be co-expressed on the same vector or independently expressed on two separate vectors. The selection process facilitates the selection of the most efficient expressers or silencers of the gene of interest.
Standard services include transfection of plasmid DNA (10 – 20 µg), drug selection of clonal cells, colony pick up, generation of a stable line, expression and functional screening (at least 10 passages), and final validation of construct expression by qRT-PCR and/or Western blot analysis.
The Western blot analytical technique uses antibodies specific to the target protein in order to quantify the expression of the protein in a sample. The genomic integration of the target gene and associated mRNA expression increase can be verified using quantitative real-time RT-PCR technique. The utilization of both techniques validate that the target gene is both integrated into the genome and functionally expressing.
Purification or isolation of nucleic acids is a critical step for most methods in molecular biology, clinical research, genomics and biotechnology. The success of your downstream applications therefore depends on rapid, reliable, and efficient DNA or RNA extraction and purification.
We offers services of extracting and purifying DNA or RNA from a vast array of cellular sources, from human tissues down to bacterial cells. We also provide high quality nucleic acid extraction kits including DNA purification, RNA purification and plasmid purification kits.
The purification of DNA, either genomic or as a plasmid, is a critical step for most methods in molecular biology, clinical research, genomics and biotechnology. The success of your downstream applications therefore depends on rapid, reliable, and efficient DNA extraction and purification.
We provide a rapid and affordable service which generates high quality and high yield DNA using our proprietary product chemistry. Using validated procedures we are able to extract DNA from a broad range of biological matrices including but not limited to:
Lymphocytes, buffy coats, plasma and sera
Frozen tissue and biopsy material
Formalin Fixed Paraffin Embedded Tissue (FFPE)
Buccal scrapes / swabs and mouthwash samples
Cultured cells (mammalian and bacterial)
Skilled scientist team uses their proprietary, resin-based system in order to obtain total RNA of the highest quality.
With the rigors of RNA research in mind, we have developed special products that effectively stabilize and preserve RNA in samples prior to purification, as well as a comprehensive portfolio of kits for the purification of high-quality, inhibitor-free RNA from essentially any sample source.
RNA purification service is well suited to isolate total RNA from many different starting materials including:
Plasma/Serum & Urine
Tissue Samples, including Fatty Tissues & brain Cells
Biopsies, including Fine Needle Biopsies, FFPE samples
LCM samples, Blood RNA
miRNA from all sample types for miRNA PCR Analysis and microarray, Plant Materials
Fungi / Yeast (Soil)
Depending on project size, biological background of the project and downstream processing requirements, we can implement an extraction service solution customized to any application using any combination of our well proven technologies.
Engineered Cell Lines by CRISPR/Cas9 and TALEN System
Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. We are pleased to announce that we are providing new services for making engineered cell lines.
Sunomix Biosciences can use CRISPR/Cas9 and TALEN System to make various forms of gene modification. We have years of experience in generating the following cell lines.
Knockout cell lines
Conditional Knockout cell lines
Site-specific knock-in cell lines
Gene Mutation cell lines
Double gene knockout cell lines
Gene tagging cell lines
Our services include:
Targeting vector construction and confirmation, and select the most efficient engineered nuclease
Stable cell clones selection
Perform transfection on target cells including HEK293 cells, CHO cells, CHO D44 cells, HeLa cells,other tumor cells, Mouse ES cells, Human ES cells, Primary cells etc. Select cell by MACS, FACS and screen gene-edited cell clones. Expand and cryopreserve the engineered cells.
Gene editing evaluation
Evaluate the target stable cell clones via one or several assays such as Western Blot, ELISA, real-time PCR,or reporter assays etc.
Gene Expression Microarray Service
We provide an integrated solution to your gene profiling needs from sample to data. The availability of array services for both Agilent and Roche NimbleGen platforms offers our customers a variety of choices in array content, density, and analysis tools. With our gene expression array service, you get data ready for publication within a very reasonable budget. Please refer to the data deliverables of our gene expression microarray service on our web site.
Custom Gene Expression Cell Lines
The overexpression of proteins is critical for many applications, including studying the function of the gene, and in functional assays and screens. Mammalian cells offer advantages in post-translational modification and protein folding that are crucial in the production of eukaryotic proteins such as antibodies, recombinant proteins etc. Stable cell lines overexpressing specific proteins have integrated GOI in the genome and are costly and vital assets for life science research.
Although the production of such protein-expressing cell lines is very important to a lot of research applications, it can be a challenge since it requires extensive cell culture experience and expertise. Our scientists bring years of extensive experience in generation of stable cell lines. We provide services for generation of stable cell lines that can overexpress virtually any protein of interest in your cell line of choice. Customers can provide their own expression cassette or choose from our extensive ORF clone collection.
Our services include:
Construct your gene of interest into either a suitable mammalian expression vector or a lentiviral vector followed by producing high titer lentivirus. Based on your requirements we choose the best strategy and corresponding vector system.
Stable cell clones selection
Perform transfection or transduction on target cells and select stable cell clones.
Gene expression evaluation
Evaluate the gene expression in stable cell clones via one or several assays such as Western Blot, ELISA, real-time PCR, or reporter assays etc.
The genes are synthesized with 100% Accuracy Guarantee.
We strives to be the most competitive gene synthesis service provider in the industry! Genes up to 1,200 bp are normally synthesized in 2 weeks!
Gene Synthesis Service Highlights
Free codon optimization upon request.
Free subcloning in one of our standard vectors (pBSK(+) Simple/pUC18/pUC19/pUC57).
Subcloning into any vector of your choice.
One stop source: from gene synthesis to protein expression and antibody production.
Gene Synthesis Deliverables, Turnaround Time and Pricing
4 µg of lyophilized plasmid containing the gene insert, sequence verified.
Project QC report, complete construct map and sequence chromatograms.
Gene Length Turnaround
<1.5 kb 2-3 weeks
1.5-3 kb 2-4 weeks
3-5 kb 4-5 weeks
5 kb 5+ weeks
Standard or Custom Vector Information
Cloning Vector Resistance
pBSK(+) Simple-Amp Ampicillin
pBSK(+) Simple-Kan Kanamycin
pBluescript II SK(+) Ampicillin
Whole Genome Mapping
Whole genome mapping is a de novo process that generates whole genome, ordered, restriction maps with no requirement for previous sequence information and provides a comprehensive view of genomic architecture. Resembling a barcode, the whole genome map is unique to the organism and provides a complete, structural view of the genome that reveals its architecture in a single, easy-to-interpret image.
Whole Genome Mapping Features and Benefits:
A more accurate approach – enables detection and correction of assembly errors for higher accuracy sequence assembly.
Without references – independent of sequence information, and do not require amplification or PCR steps.
High resolution – accelerate whole genome sequencing.
Whole Genome Mapping Applications:
Estimate the absolute length of a genome
Assemble Whole Genome Sequencing
Quickly detect differences between two genomes
CD Genomics Whole Genome Mapping Service includes:
Enzyme digestion result
Assembly and analysis
Genome-Wide Association Studies (GWAS)
Genome-wide association studies (GWAS), thousands or up to millions of genetic markers are genotyped in groups of individuals that either carries a certain trait (cases) or not (controls). Differences in the allele and genotype frequencies between the different groups lead to the detection of genomic regions that are associated with the certain trait. GWAS typically focus on associations between single-nucleotide polymorphisms (SNPs) and traits like major diseases in humans or breeding traits in animals and plants. Equipped with Affymetrix GeneChip® Scanner 3000 7G and Illumina’s iScan System, We offers researchers the flexibility to profile samples with millions of markers in high-throughput format, and deliver dense genome wide coverage with the most up-to-date content available from the scientific community.
Multi-stage Design and Analysis
SNP Positioning and Search
Disease Associated Study
Odds Ratio, Relative Risk Calculation
Linkage Disequilibrium (LD) Analysis
Why Choose Sunomix Biosciences?
Cost-effective analysis using custom arrays
Exceptional study design, data analysis options and support
Peace of mind with complete sample tracking and quality assurance
Rapid and standardized delivery of high-quality results using automated, high-throughput sample processing.
CNV Analysis Service
Agilent aCGH Technology
Agilent’s oligonucleotide aCGH platform offers genome-wide as well as customized profiling on 60-mer oligo CGH microarrays for human, mouse and rat. Agilent’s CGH end-to-end solution consists of flexible microarray formats, optimized and easy-to-use protocol, high resolution microarray scanning and powerful analytics software.
Supports a variety of formats, probes, and species
Custom arrays with user-defined content
Commercial arrays with standard content
Both Affymetrix and Illumina offer solutions for CNV analysis using SNP arrays. We provides wet lab and data analysis services for each platform.
Analyze SNP and CNV data across millions of markers
Estimate Log R ratio and B-allele frequency for copy number analysis
Call genotypes, normalize and cluster data, and generate SNP statistics
Export genotype data to various third party applications; access multiple CNV algorithms and CNV analysis tools
Generate a chromosomal heat map for examining copy number aberrations across the entire genome for multiple samples
Free advice on experimental design
Peace of mind of using a laboratory with extensive experience of running small, medium and large-scale projects
Excellent data quality
Fosmid library construction service
We offers high quality fosmid library construction.
Fosmids are commonly used for preparing genomic libraries when a smaller insert size is desired. Fosmids are excellent candidates for closing gaps in a whole genome sequencing and physical mapping, metagenomic and expression screening and more due to their uniform coverage. The entire 40 kb insert can be shuttled into an expression vector more easily than a larger insert and the higher copy number per cell allows for easier manipulation and cheaper downstream applications.
Our Fosmid library construction service as follow:
Large Fosmid Insert length Available
Exceptional Yield & Small DNA Input Required
Easy production of Fosmid DNA.
Exceptional Success Rate.
Genomic library construction
Genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. then, using a host cell to carry the vectors allowing for easy amplification and retrieval of specific clones from the library for analysis.
We accumulated extensive experience in constructing large insert genomic libraries with different clone vectors.
Mutant library construction
In vitro molecular optimization is a very efficient means of generating mutant proteins with improved or novel properties, identifying regulatory sequences, and probing for structurally and functionally critical residues. Mutant libraries constructed using the in vitro molecular optimization method provide one useful approach to the systematic study of protein properties, regulation, and function.
Our mutant library service includes:
Sunomix Biosciences combines its expertise in de novo gene synthesis and site-directed mutagenesis into an excellent site-directed mutagenesis library construction service. The site-directed mutagenesis library offers a great platform for protein function and active center studies. In these libraries, any given residue can be substituted with any of other 19 common amino acids, creating systematic combinations of amino acid mutations that reveal any significant pattern.
Scanning point mutation is a systematic means of improving protein performance. It outperforms standard alanine/cysteine scanning by replacing each amino acid with all 20 amino acids simultaneously. This technique provides a detailed profile of each amino acid at the position. For each codon of interest, a small, site-saturated library is constructed. This library can be delivered as a pool or in a separated format for any substitution variant (19 in total). The application of our expertise in de novo gene synthesis to the field of sequential permutation scanning allows us to provide superb sequential permutation scanning library construction services.
With our advanced degenerated oligonucleotide techniques, we can generate any form of randomization or degeneration of full-length gene in a synthetic DNA fragment. This permits controlled, highly precise randomization within oligonucleotides. Our in vitro library synthesis technology can introduce random substitutions on a controlled level with maximum flexibility. The mutation frequency can be set to any value between 1 and 20 mutations per kb. A peer group of 48, 96, or 192 individual transformants is sequence-verified.
BAC Library Construction Service
BAC libraries are essential elements in doing large-scale genome research such as genome sequencing, physical maps, and gene cloning. This technology was used in applications for genomic analysis that included large-scale physical mapping and genomic sequencing although this technology was developed much later than Yeast Artificial Chromosome (YAC) and cosmid cloning technologies.
This service should be attributed to the technical advantages of BAC cloning over YAC and cosmid cloning system summarized as follows:
Bigger insert size (up to 300 kb) than cosmid and other plasmid system.
Much more stable than yeast.
Bacterial clones and libraries grow much faster than YAC.
Easier in handling and gridding during DNA preparation.
Our BAC library construction service as follow:
Arrayed/non-arrayed BAC library
An arrayed or non-arrayed BAC library is the preferred choice for researchers wishing to screen using hybridization techniques. An arrayed library is also the only template that will do for whole genome sequencing or physical mapping.
Screened/Pooled BAC library
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive
Dependable and Affordable DNA Oligos and DNA Probes
We are a leading supplier of long DNA oligos. With cartridge (BAP) purification system, we deliver highly competitive DNA oligos at high purity level – shorter oligos (<20 bases) could reach as high as 98% – yet the cost is much less than HPLC or PAGE method. DNA oligos produced by the BAP cartridge method have such high purity that they can be directly used for any downstream experiments such as PCR, DNA sequencing, gene synthesis and mutagenesis.
Synthesize DNA oligos up to 180 bases.
Scale from 50nmol to 20µmol.
4 grades are available: Desalted, BAP-Cartridge, PAGE and HPLC.
Wide range oligo modification services.
50nmol BAP Cartridge
100nmol BAP Cartridge
200nmol BAP Cartridge
Quantitative PCR (QPCR), is a DNA sequence detection and quantitation method extensively used for DNA copy number detection, gene expression, SNP genotyping, adventitious agent detection, and many other applications in the fields of genomics research, drug R&D, and biologics testings. Leveraged with extensive experience in QPCR assay design, validation, and testing, and equipped with the state-of-art ABI 7900 HT Sequence Detection System, We offer a variety of Real Time PCR services to clients around the world.
Real-Time PCR Services
The following real-time QPCR services are offered based upon applications.
Copy Number Analysis Target gene specific and genomic DNA assays are used to determine the copy number of a specific gene in cell banks.
Biodistribution Studies Sensitive and target-specific QPCR assays can be custom designed and validated to assess the presence/absence of gene therapy vectors in tissues collected in a preclinical animal safety study.
Residual DNA Testing Sensitive and target-specific QPCR assays are routinely used to detect residual host cell DNA in biological products derived from cell substrates (E. Coli, CHO, Yeast, Human, etc). Residual DNA Testings is also commonly used for cleaning validation.
Gene Expression Analysis The expression levels of specific genes, Small RNA, Micro RNA, and any RNA species of different samples are analyzed using Relative Quantitation Assays predesigned by Applied Biosystems Inc. or custom designed.
TaqMan SNP Genotyping Single Nucleotide Polymorphisms (SNPs) are assessed using TaqMan Allelic Discrimination assays predesigned by Applied Biosystems Inc. or custom designed using ABI’s SNP Genotyping Assay Design Software.
Adventitious Agent Testing The presence/absence of adventitious agents, such as virus and bacteria, is determined with Plus/Minus assays that include target specific PCR primers and probes.
Custom Assay Design and Validation Target specific assays of any species are routinely designed, qualified or validated.
Validated Assay Transfer and Testing Technology transfer of clients’ validated QPCR assays for the purpose of testing a large number of samples in a cost-efficient way.
QPCR Assay Types
Both Real-Time PCR or End-Point PCR assays are routinely performed at Sunomix Biosciences.
Absolute Quantitation Assay Real-time PCR assay with absolute quantitation determined via a Standard Curve.
Relative Quantitation Assay Real-time PCR assay with relative quantitation determined as fold-chage relative to a calibrator or normalizing sample.
Allelic Discrimination Assay End-point PCR assay used to determine the genotype (homozygotes or heterozygotes) of samples.
Plus/Minus Assay End-point PCR assay used to determine the presence or absence of a specific target sequence in a sample.
QPCR Services By Quality Requirements
Sunomix Biosciences offers QPCR services under GLP and GMP regulations promulgated by US FDA (CFR 21 Part 58, Part 210 & 211 and Part 11), European, Japanese, as well as other regulatory agencies worldwide, as applicable to laboratory services provided. We have extensive experience working with clients around the world.
Research Grade QPCR DNA sequencing is performed using the same methods as GLP/GMP DNA sequencing, but with less documentation and quality unit involvement.
GLP Grade QPCR DNA sequencing is performed for the purpose of regulatory submission under Good Laboratory Practices (GLP) promulgated by US FDA (21 CFR Part 58 and Part 11), European, Japanese, and other regulatory agencies worldwide.
GMP Grade QPCR DNA sequencing is performed for the purpose of regulatory submission under current Good Manufacturing Practices (GMP) promulgated by US FDA (21 CFR Part 210 & 211 and Part 11), European, Japanese, and other regulatory agencies worldwide.
PCR Cloning and shRNA-encoding vector construction
We provide cloning services, DNA sequencing, alignment, and plasmid construction. Customers are welcome to send us the Gene ID/Genbank/Swiss-Prot number, the gene/protein sequence, or a plasmid containing a gene of interest.
shRNA Design and Synthesis
We offer a complete service for preparing DNA templates and synthesizing hairpin shRNAs. Purification of the resulting shRNAs and preparation for transfection into cultured cells are offered as well. mRNA Amplification uses the T7 promoter (tagged polydT) primer for priming first strand cDNA synthesis. Double-stranded cDNA is used as a template for in vitro transcription.
In addition to standard ribonucleotides, company scientists also incorporate modified nucleotides (e.g., aminoallyl-UTP) to produce labeled RNA probes and amplifies the original mRNA more than 1 million-fold. RNA is also purified and prepared for all additional applications.
Gene Knockdown by shRNAs
The customer provides the name and accession numbers of the target genes, mails the cells and primary antibodies for Western blotting analysis to us, and the lab team does the rest . Our service includes design, construction, and confirmation of the 3′-5′ sequence the shRNA vectors for every target gene. Design and synthesis of the SYBR-labeled qPCR primers for quantitation of target gene expression, shRNA transfection into the cell line supplied by the customer, and targeted gene expression analysis by qPCR at 24, 48, and 72 hr post-transfection and Western blot at 48 and 72 hr after transfection are performed by company scientists. Transfection controls includes the negative vector control with scrambled sequences (21 bp) and a GFP protein vector control for assessment of transfection efficiency.
Plasmid DNA preparation is a method used to extract and purify plasmid DNA. Many methods have been developed to purify plasmid DNA from bacteria. We offer both research grade plasmids and endotoxin free plasmids (endotoxin <0.1 EU/mg). Scale from 100 ug to 100+ mg, our high quality plasmids will help you speed up your research in efficient cell transfection, DNA vaccination, antibody production and other in vitro studies.
We offer a wide range of plasmid preparation services for many applications, including research, preclinical, clinical, and diagnostic applications. The plasmid DNA isolation service will be suitable for transfection and animal studies to both biotechnology researchers and pharmaceutical companies. Our goal is to provide you with the most affordable, high-quality plasmid DNA manufacturing services that ensure your satisfaction in a timely and professional manner.
Plasmid DNAs serve as important tools in genetics and biotechnology labs where they are commonly used to multiply (make many copies of) or express particular genes. Another major use of plasmids is to make large amounts of proteins from the inserted gene. This is a cheap and easy way of mass-producing a gene or the protein it then codes for, for example, insulin or even antibiotics.
Summary of Our Process:
Selection of host strain and growth conditions.
Cell harvesting, lysis and plasmid purification.
Wide range of quality control testing including genomic DNA /RNA /protein contamination test, endotoxin (lipopolysaccharides) assay, restriction profile analysis, DNA Sequencing and OD260/280 readings and so on.
We offer plasmid production services for your scientific research as follows:
Construct your plasmid according to your requirements.
Produce plasmids for applications both in vitro and in vivo.
Robust manufacturing protocols.
Flexible scale: up to gram level.
High standard quality control.
Short turn-around time.
We offer exceptional quality siRNAs to knock down your target genes. Custom siRNAs can be synthesized according to sequence information you provide, or you can take advantage of our complimentary siRNA design service*. Up to 30-mer siRNA including a choice of 32 different 3′ overhangs can be ordered with a variety of modification options for expanded specificity. siRNA is provided purified, annealed, lyophilized and ready-to-use. For even greater convenience, we offer Genome-wide Pre-designed siRNA – siRNAs pre-designed for human (18,084 genes), mouse (17,118 genes) and rat (9,392 genes) synthesized and ready-to-ship.
All custom siRNAs are synthesized in our state-of-the-art clean room facility and then purified free of charge. For higher purity, HPLC purification is available at an additional charge. Each siRNA is quality controlled by MALDI-TOF mass spectrometry to guarantee highest quality and analyzed by PAGE to confirm its duplex structure.
Features and Benefits
Guaranteed performance: Two of three custom siRNA will give 80% siRNA knockdown
Guaranteed Quality: Manufactured in a state-of-the-art clean room and QC’ed by MALDI-TOF and PAGE
Custom siRNA design service available: Turbo si-Designer software design is available free of charge
Competitive pricing: Overhang and annealing service provided free of charge.
shRNA Adenovirus Production, Full Service Pilot Scale
Recombinant Type 5 shRNA Adenovirus (E1/E3 deletion) with Ad.MAX System:
– One-stop service: from shRNA design to packaging.
– Unsurpassable high infection efficiency.
– Special design request acceptable, FREE!
Large shRNA Ad.MAX™ shuttle vector collection offers tremendous flexibility for choosing a specific promoter and reporter.
Single-promoter Ad.MAX™ shuttle vector
Dual-promoter Ad.MAX™ shuttle vector
Gene cloning and subcloning of genes into any cloning vector you want. 100% sequence guarantee
Antibodies targeted toward specific diseases or targets can be codon optimized for maximum expression in the host organism. Also, an antibody library can be constructed to screen for the most efficient antibody variant.
Organism Production Optimization
Optimize expression of genes related to resource production to maximize industrial biological production efficiency.
Get difficult-to-obtain DNA sequences without a template and upgrade the quality of your research by constructing hypothetical genes.
Codon optimization can increase protein expression efficiency, and a mutant library derived from this process can yield proteins with increased function. Optimizations include secondary structure removal, and repeat reduction as well as organism optimizations.
Sunomix Biosciences & it’s partners are the industry leading expert in antibody, protein engineering and can utilise our experience to provide comprehensive services for the conjugation and characterisation of Antibody-Drug Conjugates. With years of experience in the a variety of cell based bioassays and binding assays, we provide comprehensive expertise to support the development of antibody-drug conjugate products for all phases of drug development from discovery through to GMP manufacturing.
Antibody-drug conjugates (ADCs) are a new class of highly potent biopharmaceutical drugs designed as a targeted therapy for the treatment of people with cancer. ADCs are complex molecules composed of an antibody linked, via a stable, chemical linker to a biological active cytotoxic (anticancer) payload or drug. Therapeutic monoclonal antibodies may be conjugated with a wide array of different molecules. Using the highly specific and selective targeting mechanism of the antibody, the ADC can discriminate with great sensitivity between normal and cancerous tissues, allowing a significant reduction in the therapeutic dose of the drug reducing the toxic side effects to patients.
We provides a wide range of services to support the characterization of various forms of antibodies that can be applied to antibody drug conjugates, which including almost all the monoclonal and engineered antibodies, peptides and proteins. Our scientists are pleased to offer site-specific antibody-drug conjugation services based on traditional strategy as well as unique conjugation strategy developed at our labs. Small drugs or toxins can be conjugated to an antibody at a specific site away from the antigen binding site.
We offer a series of Antibody-Drug Conjugation Services
Conjugation of drug or toxin to antibody
Characterization of Antibody-Drug Conjugates bysize exclusion chromatography and UV/Vis spectrometry
Binding and Immunoassays
Peptide library is a systematic combination of different peptides in large numbers. With a 106-well, high throughput peptide synthesizer, we can simultaneously synthesize 106 different peptides (6-22 aa) per run at the most economical cost. Each peptide undergoes rigorous quality control to avoid any cross contaminants before delivery. This system is ideal for library peptide synthesis where a few milligrams of a large number of peptides are required at crude levels.
For peptides between 6 and 22 amino acids
Scale from 2.5µmol to 50µmol
Modifications include labeling, the incorporation of unnatural amino acids, biotin and fluorescence
No cross contamination
Standard turnaround: 2 weeks
Price per Peptide (in US Dollars)
We offer diverse while cost-effective protein conjugation services. They are a solid part of our all-in-one custom services in the field of protein expression and antibody production. Our capacity allows both small-scale and large-scale custom protein conjugation and purification. In particular, we provide a wide variety of services for conjugating amine-reactive fluorescent dyes, horseradish peroxidase, alkaline phosphatase and other enzymes, biotins and other haptens to antibodies.
For standard antibody conjugation to organic dyes and protein conjugation to other dyes, enzymes or other biomolecules. Labeling of other functional groups, special purification of conjugates and bioassays may also be available.
Features of the Services:
A full range of services from protein purification and labeling to conjugate purification
A wide variety of labels including fluorescent dyes, horseradish peroxidase, alkaline phosphatase and other enzymes, biotins and other haptens
Instant access to protein expression and antibody production services
The lowest prices in industry.
We will make our best effort to produce the conjugate you request. Optimal labeling must be determined empirically; and it is technically impossible to guarantee the yield and biological function of the final products. We refund payments for the failed services. Whatever labels you want to add to your protein, just let us know; in most cases we can accommodate your request.
Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture, which is vital for the characterization of the function, structure and interactions of the protein of interest. As an industry leader in recombinant antibody and protein production, we are pleased to launch the best protein purification service. With years of experience in purification of thousands of proteins, we have the necessary know-how to deliver the best possible results.
No matter the protein is produced at our labs or provided by the client as cell pellets, bacterial paste or conditioned medium, We can propose a solution to quickly and cost-effectively purify your proteins to your specifications.
Our protein purification services include:
Antibody Purification (Protein A/G)
Membrane Protein Purification
Non-Tagged Protein Purification
Protein Sample Preparation
Tagged Protein Purification
Our most prominent protein enrichment methodologies include:
Ion-exchange chromatography – Cation exchange, anion exchange
Gel filtration / Size-exclusion chromatography
Hydrophobic interaction chromatography
We specializes in developing novel multiplexed protein assays for immune monitoring. These assays have been designed to measure humoral immune response to biological therapeutics and vaccine antigens, vaccine carrier proteins, as well as infection. We use the Cytometric Bead Array (CBA) as an assay platform for immune monitoring. This bead-based flow cytometry application allows users to quantify multiple proteins simultaneously. We have extensive experience working with pharmaceutical companies developing CBA-based antibody detection assays. Intracellular cytokine assays we offer flow cytometry-based intracellular cytokine assays and sample processing services for immune monitoring relevant for studies of autoimmune disease and many other applications.
Sunomix Biosciences offers custom antibody production services to the scientific community to accelerate a project From Biology to Discovery™. When the antibody for a chosen antigen is not already available off the shelf, it is time to develop a custom project. We want an antibody that specifically detects the antigen in the chosen application.
Monoclonal Antibody Production Services
We offers different options to develop a monoclonal antibody with preset properties. Proteins, peptides, modified peptides, or cells can be used as antigens in mice and rats.
Protein: With 2 mg of protein as antigen, we generate a set of monoclonal antibodies against the target protein for assay development. We purified 10 mg of antibody within 5 months.
Peptide: We produce clones against a short region of the target protein. We designs and synthesizes the best peptide candidate from the protein sequence. We purify 10 mg of purified antibody within 6 months.
Modification-containing peptide: For monitoring target activation by phosphorylation for instance, a phosphopeptide is used as antigen to generate phosphospecific monoclonal antibodies. We purify 2-10 mg of antibody within 6 months.
We offers complimentary peptide design services to ensure the success of the project.
Monoclonal Antibody Services
Protein mAb Combo
Peptide mAb Combo
Modified Peptide mAb Combo
Peptide synthesis up to 20 aa, purity>90%, 15-20 mg
Phospho-peptide synthesis up to 20 aa, purity>90%, 15-20 mg
Animal immunization (5 mice), serum titration and spleen collection
Hybridoma fusion, screening & subculture
Antibody Production & Purification (2 clones up to 15 mg)
Every antibody project is supplied with a Peptide Design and Project reports, when applicable. We guarantee that the antibody generated using an antigen synthesized by us will recognize the antigen in ELISA.
Althought most projects can be completed within 5-6 months, actual processing time is dependent on the peptide synthesis, animal immunization processes and clone subculture. Please contact the Technical Service Dept for updated timelines. Estimated delivery times are stated on all quotations.
We have extensive experience in producing monoclonal antibodies in hamsters. The use of hamster as the host animal offers a unique species alternative in areas where mouse and rat antibodies may not work well. We can employ up to 10 hamsters for each antigen. Immune responses are elicited using immunogens such as transfected cell lines, DNA, recombinant proteins, native proteins, peptides or haptens conjugated to carrier molecules.
We perform splenocyte fusion on one or more animals. We select and subclone the strongly positive clones from the parental cultures by 2 rounds of limiting dilution cloning. Stable clones will be expanded into 24-well plates and then into T-flasks for further characterization. At no additional cost, client could select one clone for in vitro production of approximately 1L of supernantant. Supernatants will be purified using Protein A/G column and pure antibodies will be deliverable. We also offer a full range of contract manufacturing services, including In vitro Antibody and Protein Production in bioreactors and large-scale Antibody Purification.
Our high affinity hamster antibodies is suitable for common research applications: Chromatin Immunoprecipitation, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot.
Yeast two-hybrid cDNA library construction service
The establishment of the yeast two-hybrid library is based on the understanding of the regulation of the transcription initiation process of eukaryotic cells. Many eukaryotic transcription activation factors come from two separate, functionally independent domains. However, a separate DNA binding domain cannot activate gene transcription, and a separate transcription activation domain cannot activate the UAS downstream genes. The DNA binding domain and transcription activation domain have to be combined to derive the complete function of the transcription activation factor.
The yeast two-hybrid library can be used to study the interaction between known proteins, to find the domain that plays a key role in protein-protein interaction and to discover a new protein interaction with target proteins.
The yeast two-hybrid library has the following applications:
We are committed to assisting the research community with proper cell line documentation through its Cell Line Authentication and Mycoplasma Detection services. We offer the most comprehensive testing available. We uses DNA STR analysis, examining 17 highly polymorphic genetic markers, more than twice than is recommended. A DNA profile is generated, uniquely identifying the cell line. Authentication will ensure the research has been conducted using the correct cells, maximizing reliable results, supporting publication efforts and reducing the risk of wasted time and resources. A researchers reputation could also be adversely affected by publishing results from contaminated cell lines.
We uses STR (Short Tandem Repeat) Analysis in Cell Line Authentication.
The DNA profile generated for Cell Line Authentication utilizes STR analysis of a number of highly polymorphic unlinked loci. Each locus is characterized by a motif of repeat units typically 4 bases in length. The number of repeats detected in a DNA fragment identifies each allele. For example, if the designation at the D5S818 locus is 14, 16, two DNA fragments were detected; one with 14 repeats and one with 16 repeats. The loci used for Cell Line Authentication are utilized for human identification for paternity and forensic analysis and are not designed to detect non-human DNA. Cell lines established from different human sources will possess different DNA profiles.
We offers a comprehensive menu of Custom Immunoassay Development Services for research, diagnostic and therapeutic procedures.
We are a leading service provider that focuses on developing high specific, high affinity monoclonal antibodies for diagnostic use. We have also established proprietary antibody screening and recombinant protein standards manufacturing methods that are tailored for immunoassay development. Of note, we has extensive experience in developing immunoassay kits for quantitative detection of enzymes, antibodies, antigens, receptors, inhibitors, peptides and small molecules from cell culture supernatant, tissue homogenate, cell lysate, serum, plasma, ascites, cerebrospinal fluid and urine samples that are collected from either preclinical animal studies or clinical human studies. We can also build up kits that are intended for analysis of enzyme activity, protein-protein interaction, nucleic acid-protein interaction and inhibitor characterization.
Our staff scientists have extensive experience in screening of phage display peptide, cDNA and scFv/Fab libraries. In particular, by conducting library screening, panning, for 4 cycles, we normally get scFv/Fab antibodies of an affinity of 10-7 By constructing serial sub-libraries of the isolated scFv/Fab antibodies, Our protocol allows increase of the affinity of the scFV antibodies from 10-8 to 10-9. We have successfully obtained a scFV antibody that has an extremely high affinity of 10-12, whose binding to the antigen is essentially irreversible.
Phage Display Libraries:
Libraries commercially available
In house premade libraries (HuScL®, HuSdL®, HuFabL®, MuScL®)
Custom constructed library or libraries from the customer
Intact cells, tissues or any in vivo selection systems required by the customer.
Identify enzyme inhibitors
Map epitopes on antigens;
Select new antibodies;
Discover/validate new therapeutic targets;
Discover interaction between ligand and receptor;
Select any peptide/protein binders
Adenovirus Amplification, Large Scale
Large Scale Amplification with Ad.MAX™ System & 2xCsCl Purification:
– Exceptional gene delivery efficiency (nearly 100%) for in vivo studies
– No integration into host genome
– Induce high-level transient protein expression.
– Accommodate inserts of up to 8 kb.
Generation of high titer recombinant adenoviral stocks in ~4000 cm2 of Ad.MAX™ 293 cells.
2xCsCl ultracentrifuge for highly purified adenovirus.
Adenovirus PFU titration.
Required Materials: Customer provide adenovirus vector or select our pre-packaged adenovirus vectors.
Turnaround Time: 1 ~ 2 weeks.
Deliverables: >2.0 ml (10×0.2 ml) super purified adenovirus particles at >5E+10~5E+11 PFU/ml* suitable for in vivo studies (OK for animal injection).
Custom-Designed Studies in Virus Production
We have the expertise to grow a variety of different viruses and to bulk produce many types of tissue culture cells. Please call for additional information.
Our regularly works with clients to expand and purify viruses needed for research or other studies. In the past we worked with HHV6, MVA, Vaccinia, SHIV and many other viruses.
The capabilities exist to provide crude preparations of the virus as well as virus purified using a sucrose gradient. These capabilities allow us to provide both small and large volumes of virus at low and high titers.
shRNA Adenovirus Production, Full Service Large Scale
Recombinant Type 5 shRNA Adenovirus (E1/E3 deletion) with Ad.MAX System:
– One-stop service: from shRNA design to packaging.
– Unsurpassable high infection efficiency.
– Quick turnaround time: 3 to 4 weeks.
– Special design request acceptable, FREE!
Large shRNA Ad.MAX™ shuttle vector collection offers tremendous flexibility for choosing a specific promoter and reporter.
Single-promoter Ad.MAX™ shuttle vector
Dual-promoter Ad.MAX™ shuttle vector
CMV, CAG , H1, U6, Synapsin, UBC, EF1α, ALB(1.4), ApoE/AAT1, CaMKII
ELA1, Enh358MCK, cTNT, GFAP, MBP, SST, TBG, αMHC, hRPE, mIP1, tMCK
eGFP, RFP, mRFP, TurboGFP, eYFP, Venus, Luc, LacZ
Required Materials: Sequence of gene of interest or shRNA.
Turnaround Time: 3 ~ 4 weeks.
Deliverables: >2 ml of high purity and high titer (5E+10~1E+11 PFU/ml*) adenovirus stock suitable for in vivo studies (OK for animal injection) .
The goal is to support immunologic research related to the pathogenesis, treatment and diagnosis. The experienced staff address this goal by supporting the research endeavors with services to characterize phenotypic, functional and immunogenetic attributes of the immune system.
– Receive/procure, process, catalogue, store and distribute biospecimens
– Perform phenotypic and functional assessment of the immune system
– Perform multiplex soluble marker testing
– Perform immunogenetic testing
– Provide access to novel instrumentation
– Provide consultation and training
Services Reviews Endorsements
Enzyme-linked immunosorbent assay (ELISA)
– Sandwich, competitive, indirect, ELISpot
– Grow mammalian cells
– Optimization of protocols for commercial kits
– Analyze serum, plasma, cells, tissues
– In-house human serum/cells/tissue
– Analyze fluorescent/chromogenic substrates
-Cytometric Beads Array CBA
– Multi-channel pipets
– Automatic pipets
Number of samples processed in a single experiment: 80
Typical turn-around time: 2 days
ELISA based testing for cytokines, chemokines, soluble receptors and other blood/body fluid components.
We offer Cell Banking Services, Cell Growth and Cryopreservation Services. GLP-compliant cell line banking systems provide mammalian cell production, assurance that a uniform population of cells is preserved, cell integrity is maintained, and cells are readily available per customer request. Our cell banking service includes generation of Master Cell Bank (MCB) and Working Cell Banks (WCB). Facilities Cell line banks are maintained in a secure, controlled and monitored storage environment. Cell banking work is performed within certified Class II laminar flow biosafety cabinets with HEPA filtered air. Cells are grown only in pre-calibrated incubators with UV air and water protection. Quality Control GLP-compliant process with established SOPs and associated cell line batch records. Cell lines are certified to be free of Mycoplasma or Bacterial contamination Cell growth and Cryopreservation We provide cell expansion to manufacture a cell bank using standard T-flasks, roller bottles, shake flasks, and cell factories. Cryopreservation is performed in a monitored liquid nitrogen system. We can ship the cell bank (on dry ice) directly to the client or to a specified third party.
GLP-compliant Cryopreservation Services (Cell Banking)
Cryopreservation and cryogenic storage at ultralow temperatures provides an indefinite longevity to cells. Liquid nitrogen at -196°C is required to successfully preserve the complex biological structures and to virtually stop all biological activity. Our cell banking service complies with regulatory guidelines to ensure that all procedures, practices and facilities are conforming. Our GLP-compliant cryopreservation and cell line banking systems provide mammalian cell bank production and services.
All cell lines are certified to be free of Mycoplasma or Bacterial contamination. Our protocols utilize suitable combinations of cryoprotectants and cooling regimes to guarantee successful cryopreservation of biological materials, cells, tissues, tissue samples, including: Stable cell lines, Cells for transfusion, Umbilical cord blood cells, Stem cells, Tumor/ histological cells. We offer long-term storage of cells in a secure, controlled, and monitored environment. Cell banking work is performed within certified Class II laminar flow biosafety cabinets with HEPA filtered air. Cells are grown only in calibrated incubators with UV air and water protection. Banks are divided and stored in separate freezers to minimize risks of a freezer malfunction. The levels of the liquid nitrogen and freezer temperatures are monitored 24/7 via the monitoring system. We ensure the security of the storage facility by a fully redundant backup system. Cryovials can be used (to expand cell population) or shipped directly to the client or to a specified third party. We ship cells on dry ice to any destination worldwide.
Clinical Molecular Diagnostics Services
Biochemistry & Molecular Biology Services
Human Biospecimen Management and Storage Services
Pharmacology & Toxicology Services
• Acute Toxicity
• Subchronic Toxicity
• Chronic Toxicity
• Range Finding
• Behavioral Studies
• Maximum Tolerated Dose
• Reproductive Toxicity
• Carcinogenicity Studies
Sunomix Biosciences has not received any reviews.
Sunomix Biosciences has not received any endorsements.