Our laboratory answers biochemical and structural biology questions in fields related to cancer research with a focus on transcriptional and translational cellular processes. We believe that a better molecular and structural understanding of these processes will reveal novel cancer drug targets. We therefore generate high quality protein reagents and conduct biophysical, biochemical, and structural characterization experiments to uncover novel mechanisms of action. And within integrated teams of clinicians, biologists, and chemists, we perform in-vitro studies that facilitate development of novel chemical probes, tool compounds, and drug therapies. If you are looking for a partner and collaborator on your mechanism- or therapeutic-focused project, don't hesitate to contact us.
Crystal screening, optimization, and harvesting. Data-collection, data-processing, phasing, structure refinement, and PDB deposition.
Bacterial (E. coli) and Baculoviral (Sf9 and Hi5) expressions, from 1 to 10 liter scale
Milligram-scale, FPLC-based affinity chromatography (Nickle-, Flag-, and Maltose-based resins), ion-echange, chromatography (Mono-Q or -S), and size-exclusion chromatography (Superdex-75 or -200).
Isothermal Titration Calorimetry (ITC), thermal stabilization assay (TSA), differential scanning fluorimetry (DSF), Circular Dichroism (CD), Dynamic Light Scattering (DLS). Fluorescence Polarization (FP), Fluorescence Resonance Energy Transfer, Time-Resolved (tr-FRET), and ADPGlo.
See this link for options: https://www.dhepaglab.org/data/b2efaeed.html
Structural Biology Facility (DFCI) has not received any reviews.
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