StemCore Laboratories is a high-throughput genomics facility within the Ottawa Hospital Research Institute (OHRI). StemCore Laboratories is developing a world-class infrastructure for genomics, bioinformatics, and proteomics, and is capable of facilitating large-scale scientific research and biotechnology projects. Stemcore Laboratories seeks out projects that are challenging, cutting-edge, extend the boundaries of biological knowledge, and will positively impact the state of human health.
Services Provided: we provide access to the full line of Affymetrix DNA Analysis products including 500K SNP Mapping Assay Chip and Tiling Arrays
Services Provided: we provide access to the full line of Affymetrix Gene Expression products including: Human Gene 1.0 ST $450, Human Exon 1.0 ST $850, Human Promoter 1.0R $850, Mouse Gene 1.0 ST $450, Mouse Exon 1.0 ST $850, Mouse Promoter 1.0R $850, Rat Gene 1.0 ST $450, Rat Exon 1.0 ST $850, New Model Organism Chips (Xenopus $650, Zebrafish $850, C.elegans $650 etc.)
The BeckmanCoulter MoFlo can be used for multi-parameter experiments and applications requiring high speed sorting and/or sorting of four sub-populations simultaneously. A designated operator processes the samples. $50 setup plus $100 per hour.
Illumina GAIIx Genome Analyzer:
Cluster generation single read 51 cycle sequencing $1300, Cluster generation paired-end 51 cycle sequencing $1970, Cluster generation single read 76 cycle sequencing $1500, Cluster generation paired-end 76 cycle sequencing $2300
ChIP-seq Library Construction $650, mRNA Library Construction $650, gDNA Library Construction $750, Directional mRNA Library Construction $1100, Small RNA Library Construction $50
Applied Biosystems 3730 DNA Analyzer. Services: SNP Analysis, Methylation Status Detection
High throughput, automated DNA sequencing services are currently provided for by the use of the state of the art Applied Biosystems 3730 DNA Analyzer. This capillary based instrument has the capability to run up to 48 samples in one run with read lengths of greater that 1,000 bases (PHRED Q20 scores >800) within 2 hours. Services provided: DNA sequencing of plasmids, PCR products, cosmids, Phage, BAC, and Genomic DNA; Primer Walking Projects including Primer Design, Contig Assembly and Consensus Sequence Generation; High Throughput DNA Sequencing Projects
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