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Stem Cell Core Facility

Los Angeles, California, US

The USC Stem Cell Core facility is a CIRM funded comprehensive support center and central hub for the provision of knowledge, resources and state of the art technology to researchers working with stem cells. Our primary aims are to facilitate stem cell and biomedical research at USC and the LA area and to develop new technologies for working with pluripotent stem cells. We offer a wide range of services in the following areas:

Cells and Reagents
Reproducibility in experimental science is dependent on homogeneity and consistency. One of the primary aims of the USC Stem Cell Core is to standardize stem cell research by providing quality controlled cells and reagents.

Equipment
The USC Stem Cell Core is a shared laboratory space housing communal cell culture workstations, a state of the art imaging core, spectral karyotyping facilities, a Q-PCR machine, fully equipped molecular biology work stations and... Show more »

The USC Stem Cell Core facility is a CIRM funded comprehensive support center and central hub for the provision of knowledge, resources and state of the art technology to researchers working with stem cells. Our primary aims are to facilitate stem cell and biomedical research at USC and the LA area and to develop new technologies for working with pluripotent stem cells. We offer a wide range of services in the following areas:

Cells and Reagents
Reproducibility in experimental science is dependent on homogeneity and consistency. One of the primary aims of the USC Stem Cell Core is to standardize stem cell research by providing quality controlled cells and reagents.

Equipment
The USC Stem Cell Core is a shared laboratory space housing communal cell culture workstations, a state of the art imaging core, spectral karyotyping facilities, a Q-PCR machine, fully equipped molecular biology work stations and a variety of equipment for performing histological analysis. Flow cytometry and high content screening equipment are made available through the FACS core.

Laboratory Space
As a shared research facility we offer communal laboratory space for investigators to conduct collaborative or start up research involving stem cells. Our goal is to expand stem cell research in the LA area and to encourage collaboration between novice and seasoned researchers.

Technical Assistance
The USC Stem Cell Core is staffed with a team of highly experienced technicians, boasting a combined technical expertise of over 60 years. We provide comprehensive assistance, which includes a biobanking and histology service.

Training and Education
Training and education are pivotal to the advancement of Stem Cell Research. The USC Stem Cell Core offers a broad range of educational services including technical protocols, one on one customized training and a week long course covering human pluripotent stem cell culture techniques.

Research and Development
The USC Stem Cell Core Research and Development program was established with two main aims: 1) to develop and centralize new technologies for working with human pluripotent stem cells, 2) to support researchers embarking on collaborative and pilot projects.

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Stem Cell Core Facility has not listed any services.

Biobanking
Price on request

A range of services for cryopreserving hPSC's and other in vitro cell lines are available through the USC Stem Cell Core.

A range of services for cryopreserving hPSC's and other in vitro cell lines are available through the USC Stem Cell Core.

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Cell Culture Media and Reagents
Price on request

The USC Stem Cell Core distributes high quality lot tested serum from hyclone and KnockoutTM Serum replacer, a serum free formulation which is commonly used to maintain human and murine pluripotent stem cells. We offer complete media for maintaining human pluripotent stem cells with and without MEF feeders. Discounted antibodies... Show more »

The USC Stem Cell Core distributes high quality lot tested serum from hyclone and KnockoutTM Serum replacer, a serum free formulation which is commonly used to maintain human and murine pluripotent stem cells. We offer complete media for maintaining human pluripotent stem cells with and without MEF feeders. Discounted antibodies for detecting undifferentiated human pluripotent stem cells are available through the USC Stem Cell Core. We highly recommend the combined use of GCTM2 and TG30 during cell sorting procedures for isolating very primitive stem cell populations for cells at various stages of differentiation.

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Custom Cell Culture Media
Price on request

The USC Stem Cell Core distributes high quality lot tested serum from hyclone and KnockoutTM Serum replacer, a serum free formulation which is commonly used to maintain human and murine pluripotent stem cells. We offer complete media for maintaining human pluripotent stem cells with and without MEF feeders. Discounted antibodies... Show more »

The USC Stem Cell Core distributes high quality lot tested serum from hyclone and KnockoutTM Serum replacer, a serum free formulation which is commonly used to maintain human and murine pluripotent stem cells. We offer complete media for maintaining human pluripotent stem cells with and without MEF feeders. Discounted antibodies for detecting undifferentiated human pluripotent stem cells are available through the USC Stem Cell Core. We highly recommend the combined use of GCTM2 and TG30 during cell sorting procedures for isolating very primitive stem cell populations for cells at various stages of differentiation.

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Embryonic stem cell culture training
Price on request

The USC Stem Cell Core offers a comprehensive training courses in human pluripotent stem cell (hPSC) culture techniques. The courses are designed for researchers and post graduate students who are interested in establishing a hPSC laboratory and transitioning into the hPSC field. Covered topics include:

Media production... Show more »

The USC Stem Cell Core offers a comprehensive training courses in human pluripotent stem cell (hPSC) culture techniques. The courses are designed for researchers and post graduate students who are interested in establishing a hPSC laboratory and transitioning into the hPSC field. Covered topics include:

Media production (regular growth media and MEF-CM)
Matrigel coating
hPSC subculture (manual and enzymatic)
Culture of ESI serum adapted hESC lines
Feeder free hPSC culture (mTeSR1, StemPro and MEF-CM)
Techniques for plating dissociated hPSC's
EB production (via scraping and centrifugation)
Primary MEF isolation
Fibroblast culture
MEF Feeder layer production
Quality control testing hPSC cultures

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Grant Writing
Price on request

The USC Stem Cell Core is highly supportive of grant writing activities aimed at securing funding for stem cell research, particularly research involving human pluripotent stem cells and/or the Core facility. We will provide letters of recommendation and participate in collaborative grant writing upon request. Please consult... Show more »

The USC Stem Cell Core is highly supportive of grant writing activities aimed at securing funding for stem cell research, particularly research involving human pluripotent stem cells and/or the Core facility. We will provide letters of recommendation and participate in collaborative grant writing upon request. Please consult with the Core director Dr. Victoria Fox (victorif@med.usc.edu) for further details.

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Histology & Pathology Services
Price on request

The USC Stem Cell Core histology services offers assistance with processing, embedding, sectioning, H&E staining and equipment usage.

The USC Stem Cell Core histology services offers assistance with processing, embedding, sectioning, H&E staining and equipment usage.

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Human embryonic stem cell lines
Price on request

The USC Stem Cell Core distributes two of the most commonly used human embryonic stem cell lines, H1 and H9 with MEF feeders and under feeder free conditions (on Matrigel in MEF conditioned medium).

The USC Stem Cell Core holds a repository of cryopreserved cell lines, which are distributed as frozen vials or thawed cultures.

The USC Stem Cell Core distributes two of the most commonly used human embryonic stem cell lines, H1 and H9 with MEF feeders and under feeder free conditions (on Matrigel in MEF conditioned medium).

The USC Stem Cell Core holds a repository of cryopreserved cell lines, which are distributed as frozen vials or thawed cultures.

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Mouse Embryonic Fibroblast Feeder
Price on request

MEF feeders can be obtained from the USC Stem Cell Core as irradiated cells (live and frozen) or pre-plated feeder layers.

MEF feeders can be obtained from the USC Stem Cell Core as irradiated cells (live and frozen) or pre-plated feeder layers.

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Cell Culture Consulting
Price on request

The USC Stem Cell Core provides technical assistance and guidance to researchers working with pluripotent stem cells and other in vitro cell lines. To date we have provided support to over 25 labs on a range of issues including:

Basic cell culture, Stem cell culture,Experimental design, Assay development,Data... Show more »

The USC Stem Cell Core provides technical assistance and guidance to researchers working with pluripotent stem cells and other in vitro cell lines. To date we have provided support to over 25 labs on a range of issues including:

Basic cell culture, Stem cell culture,Experimental design, Assay development,Data analysis,Pluripotency testing, Sorting and plating of stem cells for experimental analysis, New cell line development (from frozen stocks and primary material), Media testing

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Editorial Support
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Clinical Laboratory Services
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Cell and Tissue Culture
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Cells and Tissues
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Biology
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Mammalian Cell Culture
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Human Biospecimen Management and Storage
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Scientific Communication
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Project Management & Consulting Services
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Biospecimens
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Clinical and Anatomic Pathology
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Clinical Research
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Cell Lines
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