Metabolomics is the global profiling of small metabolites such as amino acids, glycolytic, TCA and pentose cycle metabolites, and in combination with lipidomics (fatty acids, acyl carntines, phospholipids, di- and tri-glycerides) presents a powerful approach to functional biology for biomedical, clinical questions. Fluxomics uses stable isotopes to assess intra- and inter-organ flux. Use of fluxomics combined with metabolomics yields a comprehensive picture of metabolic regulation, and how its is impacted by disease.
The newly established Einstein Stable Isotope & Metabolomics Core (SIMC) uses stable isotope flux and metabolite profiling to help formulate and test hypotheses about the metabolic consequences of various changes in gene expression and protein function, in order to guide further integrative systems biology analyses of the underlying mechanisms in diabetes, insulin resistance, obesity, and diabetic complications. The SIMC Facility is a nonprofit organization established at the Einstein Diabetes Center. It is designed to provide services on the recharge basis. SIMC’s evaluation framework complements the analyses performed in the Animal Physiology Core, such as measurements of whole body carbohydrate/fatty acid oxidation, energy expenditure, tissue-specific insulin sensitivity, glucose, protein and fatty acid metabolism.
Assessments of plasma and tissue metabolite profiles for diagnosis/characterization of physiological and pathophysiological states. These include key metabolites in the glycolytic, gluconeogenic, pentose phosphate, and tricarboxylic (TCA) cycle pathways, fatty acid oxidation and complex lipids.
In vivo stable isotope substrate flux assays to determine rates of protein synthesis, lipogenesis and peripheral glucose disposal.
In vitro assessments of glycolysis (extracellular acidification rates) and mitochondrial oxygen consumption (mitochondrial respiration) in tissue explants, primary isolated and tissue culture cells using Seahorse Biosciences Flux Analyzers.
Stable Isotope and Metabolomics Core has not received any reviews.
Stable Isotope and Metabolomics Core has not received any endorsements.