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Spectroscopy and Biophysics Core

200 Orders Completed
Lincoln, Nebraska, US

About Spectroscopy and Biophysics Core

The role of the Spectroscopy and Biophysics Core is to provide instrumentation, training and support for any type of experimental work involving biophysical and spectroscopic measurements. This include CD spectroscopy, ITC, DSC, stopped flow, fluorescence/chemiluminescence, elemental analysis, metabolomics... Show more »

The role of the Spectroscopy and Biophysics Core is to provide instrumentation, training and support for any type of experimental work involving biophysical and spectroscopic measurements. This include CD spectroscopy, ITC, DSC, stopped flow, fluorescence/chemiluminescence, elemental analysis, metabolomics (targeted small molecule) and proteomics (protein ID, gel extraction, targeted analysis and PTMs).

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Our Services (33)


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MALDI Tissue Imaging

Matrix-Assisted Laser Desorption Ionization Tissue Imaging
Price on request
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ICP-MS

Inductively Coupled Plasma Mass Spectrometry
Price on request

Determination of most elements in the Periodic Table is possible, except for most halogens, first row elements and some non-metal elements such as S and As where the analysis is only possible if the concentration is at the mg/L (ppm) and ug/L (ppb) respectively. Instrument is equipped with a collision cell for the removal of... Show more »

Determination of most elements in the Periodic Table is possible, except for most halogens, first row elements and some non-metal elements such as S and As where the analysis is only possible if the concentration is at the mg/L (ppm) and ug/L (ppb) respectively. Instrument is equipped with a collision cell for the removal of polyatomic interferences. A single sample injection requires about 200 uL, although less volume is possible if the sensitivity is not compromised. Detection of low levels of Selenium is possible using the reaction mode with Hydrogen gas, down to sub-ppb levels.

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Protein Identification

Starting at $60.00 per sample

Tryptic (or other proteases) peptide fragmentation by LC-MS/MS. The instrument is an LTQ Velos Pro equipped with dual pressure ion-trap. The nanoLC is a 3000 Ultimate from Dionex, which includes a PepMap C18 trap column and 0.075x150 mm nanospray column for separation of peptides. Typical resolution for the MS is 10,000 (M/deltaM).

Tryptic (or other proteases) peptide fragmentation by LC-MS/MS. The instrument is an LTQ Velos Pro equipped with dual pressure ion-trap. The nanoLC is a 3000 Ultimate from Dionex, which includes a PepMap C18 trap column and 0.075x150 mm nanospray column for separation of peptides. Typical resolution for the MS is 10,000 (M/deltaM).

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CD Spectroscopy

Circular dichroism spectroscopy
Starting at $20.00 per sample

The instrument is a Jasco J850 is equipped with a Peltier thermal cell holder (5 to 90 oC), a titrator for protein and peptide denaturation studies, and a fluorescence anisotropy add-on for the study of rotational correlation of macromolecules and FRET type of experiments.

The instrument is a Jasco J850 is equipped with a Peltier thermal cell holder (5 to 90 oC), a titrator for protein and peptide denaturation studies, and a fluorescence anisotropy add-on for the study of rotational correlation of macromolecules and FRET type of experiments.

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MS-MS

Tandem Mass Spectrometry
Starting at $30.00 per sample

LC-SRM and LC-MS of small molecules ( <2000 Da) utilizing an Agilent LC1200 coupled to a diode array detector and an Sciex Hybrid QTrap 4000. The instrument can operate as a Triple Quadrupole MS as well as an ion Trap MS instrument for structural studies. The Ion Trap mode is limited to MS/MS/MS and other experiments such as high resolution (0.4Da) , and determination of charge states (ER).

LC-SRM and LC-MS of small molecules ( <2000 Da) utilizing an Agilent LC1200 coupled to a diode array detector and an Sciex Hybrid QTrap 4000. The instrument can operate as a Triple Quadrupole MS as well as an ion Trap MS instrument for structural studies. The Ion Trap mode is limited to MS/MS/MS and other experiments such as high resolution (0.4Da) , and determination of charge states (ER).

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Agilent 1200

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Isothermal Titration Calorimetry (ITC)

Starting at $124.00 per sample

Determination of binding parameters (number of sites, enthalpy, entropy and binding constant) are possible for small ligand/macromolecule systems. The required volumes are about 500 uL of titrant and 2 mL of macromolecule, although it is feasible to perform the experiment in reverse for precious protein samples. The... Show more »

Determination of binding parameters (number of sites, enthalpy, entropy and binding constant) are possible for small ligand/macromolecule systems. The required volumes are about 500 uL of titrant and 2 mL of macromolecule, although it is feasible to perform the experiment in reverse for precious protein samples. The concentrations required are dependent on the heat release/taken up at each titration point.

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LC-ESI-MS/MS

Liquid Chromatography Coupled Electrospray Ionization Tandem Mass Spectrometry
Starting at $60.00 per hour

Small molecule analysis via using HPLC coupled with Electrospray Ionization MS^n (up to 10) with Thermo LCQ Fleet Instrument. Direct infusions are also possible

Small molecule analysis via using HPLC coupled with Electrospray Ionization MS^n (up to 10) with Thermo LCQ Fleet Instrument. Direct infusions are also possible

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MALDI FT-ICR

Matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance
Starting at $60.00 per run

High Resolution (ICR) analysis of small molecules and peptides using 384-Target Analysis. The instrument is a Bruker Solarix XR. Choices of MALDI matrices are available.

High Resolution (ICR) analysis of small molecules and peptides using 384-Target Analysis. The instrument is a Bruker Solarix XR. Choices of MALDI matrices are available.

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Analytical Ultracentrifugation (AUC)

Starting at $125.00 per run

Academic rate: $100.00/run
Industry rate: $125.00/run

Academic rate: $100.00/run
Industry rate: $125.00/run

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Differential Scanning Calorimetry (DSC)

Starting at $60.00 per sample

The instrument operates in the range of 10-130 oC. The sample amount for a single experiment is about 700 uL, though 1.0 mL is usually preferable. The concentration of macromolecule that will be optimal depends on the heat of denaturation.

The instrument operates in the range of 10-130 oC. The sample amount for a single experiment is about 700 uL, though 1.0 mL is usually preferable. The concentration of macromolecule that will be optimal depends on the heat of denaturation.

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Rapid Chemical Quench

Starting at $60.00 per hour
Request a quote for more information about this service.

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FRET

Fluorescence resonance energy transfer
Starting at $60.00 per hour
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Stopped Flow Spectroscopy

Starting at $60.00 per hour
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Elemental Analysis

Starting at $60.00 per hour

Determination of most elements in the Periodic Table is possible, except for most halogens, first row elements and some non-metal elements such as S and As where the analysis is only possible if the concentration is at the mg/L (ppm) and ug/L (ppb) respectively. Instrument is equipped with a collision cell for the removal of... Show more »

Determination of most elements in the Periodic Table is possible, except for most halogens, first row elements and some non-metal elements such as S and As where the analysis is only possible if the concentration is at the mg/L (ppm) and ug/L (ppb) respectively. Instrument is equipped with a collision cell for the removal of polyatomic interferences. A single sample injection requires about 200 uL, although less volume is possible if the sensitivity is not compromised. Detection of low levels of Selenium is possible using the reaction mode with Hydrogen gas, down to sub-ppb levels.

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Imaging & Spectroscopy

Price on request
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Other Mass Spectrometry Methods

Price on request
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Tandem Mass Spectrometry Methods

Price on request
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Calorimetry

Price on request
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MALDI Mass Spectrometry Methods

Price on request
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Microscopy

Price on request
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Physical Analysis Methods

Price on request
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Spectroscopy

Price on request
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Protein Sequencing

Price on request
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Optical Microscopy

Price on request
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Centrifugation

Price on request
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Liquid Chromatography Coupled Mass Spectrometry Methods

Price on request
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Protein Services

Price on request
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Mass Spectrometry

Price on request
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Fluorescence-Based Microscopy

Price on request
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Separation/Purification Services

Price on request
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Fluorescence Spectroscopy

Price on request
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Hydrolyzed Amino Acid Analysis

Price on request
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LC-MS

Liquid Chromatography-Coupled Mass Spectrometry
Price on request
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Javier Seravalli

Spectroscopy & Metabolomics Director
  • Positive review for Analytical Ultracentrifugation (AUC):

    September 2019

    "Responsive, thorough, quick turn-around, and good price. Highly recommended."

  • Positive review for ICP-MS:

    March 2019

    "Always very professional and reliable. Easy to work with."

  • Positive review for ICP-MS:

    January 2019

    "Working with the Spectroscopy and Biophysics Core was extremely easy and the customer service was second to none. Instructions were very clear, turnaround time was incredible and when asked about several variables during our ICP-MS trial, Javier responded quickly and concisely. Plan to use more in the future and would recommend to anyone looking for efficient and very reasonably priced ICP-MS analysis."

  • Positive review for ICP-MS:

    October 2018

    "Yes. Very professional and easy to work with. Answers questions and explanations in a timely manner."

  • Positive review for LC-MS:

    October 2018

    "Javier is great and easy to work with. Very fast responses, easy to read reports, and is reliable. My future LCMS needs will go to him. Thanks, Javier."

  • Positive review for ICP-MS:

    September 2018

    "Javier was very responsive and patient when it took far longer than I expected to prep the samples. The precision of the measurements was in line with the QA/QC data he provided to me before we contracted to work together and was more than sufficient to answer the questions that we were trying to answer with these samples. This lab comes with my highest recommendation and I look forward to working with them again."

  • Positive review for ICP-MS:

    August 2018

    "Javier puts a lot of effort into running my samples even when there are mistakes made on my part. He communicates very well and I highly recommend his services."

  • Positive review for ICP-MS:

    June 2018

    "These folks are very easy to work with and have done very good work on a large number of orders over the past 18 months. I highly recommend them and plan on working with them in the future."

  • Positive review for ICP-MS:

    April 2018

    "Their turnaround time has been decreasing over the past few months (which is too bad) but I would still go with them"

  • Positive review for ICP-MS:

    April 2018

    "Always very professional and easy to work with. I highly recommend."

  • Positive review for ICP-MS:

    March 2018

    "Javier was very helpful with my questions as always"

  • Positive review for ICP-MS:

    March 2018

    "Easy to work with and high rate of accuracy on ICP-MS results."

  • Positive review for ICP-MS:

    February 2018

    "Timely responses and results"

  • Positive review for ICP-MS:

    January 2018

    "Javier always does a good job of offering quotes and returning results in a timely fashion."

  • Positive review for ICP-MS:

    January 2018

    "Generally fast turn-around and they are very conscientious about their work and lab results. I would use them again and would recommend their services to my colleagues."

  • Positive review for LC-ESI-MS/MS:

    September 2017

    "Put extra time and effort to deliver informative results."

  • Positive review for ICP-MS:

    August 2017

    "As always, a fine job and internal checks show remarkable repeatability on data."

  • Positive review for ICP-MS:

    July 2017

    "Fairly rapid turn-around, reliable data as determined by internal blind duplicates."

  • Positive review for LC-ESI-MS/MS:

    July 2017

    "I sure would want to work with Spectroscopy and Biophysics Core again. They put all their effort and time to clarify their results for you. I'm planning to send two more samples for testing."

  • Positive review for ICP-MS:

    June 2017

    "Internal controls met and work was performed in a timely and satisfactory fashion. I would highly recommend this lab for future work."

  • Positive review for ICP-MS:

    June 2017

    "They are always very prompt with quotes as well as analysis of my samples. Javier is very thoughtful about getting the details of my order correct. I will continue to work with them."

  • Positive review for ICP-MS:

    June 2017

    "They were quick, courteous, and detailed with results."

  • Positive review for ICP-MS:

    March 2017

    "Yes. Performed analysis in a timely and professional manner"

  • Positive review for Protein Identification:

    March 2017

    "Sent three protein (in gel) samples for protein ID. Javier was very timely and helpful with processing and analysis. Would definitely recommend for anyone looking to for quick MS analysis."

  • Positive review for ICP-MS:

    February 2017

    "Very satisfied. Fast turn-around and no hassles."

  • Positive review for ICP-MS:

    January 2017

    "Great fast turnaround service at an affordable price. Will certainly use their ICP/MS services in the future."

  • Positive review for Isothermal Titration Calorimetry (ITC):

    October 2016

    "Yes I would highly recommend to work with Spectroscopy and Biophysics Core again. Did a great job in delivering and explaining the results for me. Regards, Thorunn."

  • Positive review for ICP-MS:

    May 2016

    "Great job. Good data at a very reasonable price. The hourly billing for multiple measurements made it affordable to get good statistics. Recommend."

  • Positive review for ICP-MS:

    February 2016

    "Very fast and competent. I would use this lab again in the future."

  • Positive review for LC-ESI-MS/MS:

    September 2015

    "Dr. Seravalli is very professional and knowledgeable. He is able to solve complex problems and help clients gain valuable information. Thank you very much.."

  • Positive review for MS-MS:

    May 2015

    "The Laboratory Professional who had my case (Javier Seravalli) did an outstanding job, as he has in the past. Not only did he perform the requested chemical analysis, but he helped me to improve my experiment design, and provided a clear description of the tests run, and also correlated the data to theoretical values. I couldn't have asked for more; the fees were incredibly reasonable."

  • Negative review for Isothermal Titration Calorimetry (ITC):

    November 2014

    "Very unsatisfactory work. The laboratory personnel did not handle my samples well and so missed out on an important control without even caring to double check with me. He also refused to answer my emails and any further communications requiring details of the experimental procedures. Would not recommend this lab to anyone."

  • Positive review for MS-MS:

    November 2014

    "Great scientist! He was helpful and was able to break the process down to me in laymans terms. He was prompt with his responses and went above and beyond to get me quality results."

  • Positive review for MS-MS:

    August 2014

    "Detailed report did it right from the start very happy"

  • Positive review for Isothermal Titration Calorimetry (ITC):

    January 2014

    "Our experience has been good although the experiment could have been expedited. We would also like to be more involved in the experimental design before it is finally run."

  • Positive review for ICP-MS:

    October 2013

    "Very professional"

  • Positive review for ICP-MS:

    January 2013

    "I was content with the turnaround time of the project and prompt response to emails."

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    Paul FinmanMay 2016

    Great job in ICP-MS. Good data at a very reasonable price. The hourly billing for multiple measurements made it affordable to get good statistics. Recommend and endorse.

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