SOLVE Research and Consultancy AB

Method development for asymmetric flow field- flow fractionation (AF4) and size exclusion chromatography (SEC).

Detection: multi-angle light scattering (MALS), refractive index detection (RI), ultraviolet (UV) or fluorescence detection.

Sample type: peptides, proteins, antibodies, liposomes, nanoparticles, viruses like particles, vaccine, polymer, and polysaccharides. 

Turnaround time: the analysis will start within 1-2 weeks from sample arrival. Depending on the extend of the project a final report will be delivered within 4-6 weeks from start of the analysis. (Note: Timing is a guide only. Please contact us for a customized planification).

Deliverables:

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

Separation using asymmetric flow field- flow fractionation (AF4), size exclusion chromatography (SEC) and high-performance liquid chromatography (HPLC).

Detection: multi-angle light scattering (MALS), refractive index detection (RI), ultraviolet (UV) or fluorescence detection, and label-free detection methods (SPR).

Sample type: peptides, proteins, antibodies, liposomes, nanoparticles, viruses like particles, vaccine, polymer, and polysaccharides. Carbohydrates, alcohols, and organic acids in food, biochemical, biofuel, biomedical, and biotechnology applications.

Turnaround time: the analysis will start within 1-2 weeks from sample arrival. Depending on the extend of the project a final report will be delivered within 4-6 weeks from start of the analysis. (Note: Timing is a guide only. Please contact us for a customized planification).

Deliverables:

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

Separation using asymmetric flow field- flow fractionation (AF4) and size exclusion chromatography (SEC).

Methods: AF4 or SEC connected to multi-angle light scattering (MALS), refractive index detection (RI), ultraviolet (UV) or fluorescence detection, and label-free detection methods (SPR).

Sample type: peptides, proteins, antibodies, liposomes, nanoparticles, viruses like particles, vaccine, polymer, and polysaccharides.

Turnaround time: the analysis will start within 1-2 weeks from sample arrival. Depending on the extend of the project a final report will be delivered within 4-6 weeks from start of the analysis. (Note: Timing is a guide only. Please contact us for a customized planification).

Deliverables:

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

Chromatography consulting services using asymmetric flow field- flow fractionation (AF4), size exclusion chromatography (SEC) and high-performance liquid chromatography (HPLC).

Detection: multi-angle light scattering (MALS), refractive index detection (RI), ultraviolet (UV) or fluorescence detection.

Sample type: peptides, proteins, antibodies, liposomes, nanoparticles, viruses like particles, vaccine, polymer, and polysaccharides. Carbohydrates, alcohols, and organic acids in food, biochemical, biofuel, biomedical, and biotechnology applications.

Turnaround time: the analysis will start within 1-2 weeks from sample arrival. Depending on the extend of the project a final report will be delivered within 4-6 weeks from start of the analysis. (Note: Timing is a guide only. Please contact us for a customized planification).

Deliverables:

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

A typical use of asymmetric flow field-flow fractionation (AF4) at SOLVE is for size and molar mass determination. It is applicable to samples with size above ~3 nm / 10 kDa. AF4 is also applicable to aggregate analysis and to the analysis of large polydisperse compounds with tendency to aggregate or adsorb to surfaces. AF4 is as an orthogonal technique to size exclusion chromatography (SEC). One recent application of AF4 is the analysis of protein and antibodies in complex matrixes such as serum and plasma. At SOLVE we use AF4 instrumentation from Wyatt Technology, coupled to UV, RI, MALS, and FL detectors.

Applicability:

AF4 is a method applicable to a very wide range of compounds, such as proteins, nanoparticles, viruses, and polymers. AF4 is applicable to compounds with molecular weight of ~ 10 000 g/mol and upwards to compounds of approximately 1 µm in size. Typical uses are for stability testing of proteins, aggregation analysis, batch to batch control, size and molar mass distribution.

Sample type: peptides, proteins, antibodies, liposomes, nanoparticles, viruses like particles, vaccine, polymer, and polysaccharides.

Sample requirements: Dispersed sample, minimum 100 ug sample.

Turnaround time: the analysis will start within 1-2 weeks from sample arrival. Depending on the extend of the project a final report will be delivered within 4-6 weeks from start of the analysis. (Note: Timing is a guide only. please contact us for a customized planification).

Deliverables: 

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

https://www.solveresearch.com/techniques/af4/

A typical use of size exclusion chromatography (SEC) at SOLVE is as a high resolution quantitative method for monitoring degradation and oligomerization. Our SEC instrumentation is connected to MALS-RI detectors to enable direct and reliable molar mass determination, as an alternative to molar mass determination with standards. We have columns from different manufacturers to cover the size range from peptides to large polymers. We also use UV and FL detection.

Applicability: Size exclusion chromatography (SEC) is applicable to a wide range of compounds, such as peptides, proteins, and polymers, with molecular weight of ~ 1000 g/mol up to ~10·106 g/mol. Typical uses are for stability testing of proteins, aggregation analysis, batch to batch control, molar mass distribution.

Turnaround time: the analysis will start within 1-2 weeks from sample arrival. Depending on the extend of the project a final report will be delivered within 4-6 weeks from start of the analysis. (Note: Timing is a guide only. please contact us for a customized planification).

Deliverables:

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

https://www.solveresearch.com/techniques/sec/

We primarily use analytical ultra centrifugation (AUC) for validation purposes, and for the analysis of especially challenging samples after other characterization techniques had been tried (SEC and AF4). We operate AUC instrumentation with a wide range of rotors and cells, where the sedimentation of components is monitored by UV and interference optics (RI).

Applicability: Analytical ultra centrifugation (AUC) is a method applicable to a very wide range of compounds, such as proteins, nanoparticles, viruses, and polymers. The technique is applicable to compounds with molecular weight of ~ 1 000 g/mol and upwards. AUC is the golden method to compare against when it comes to validation of methods (alternately to size exclusion chromatography (SEC)). Typical uses of AUC are stability testing of proteins, aggregation analysis, batch to batch control, molar mass distribution.

Turnaround time: the analysis will start within 2 weeks from sample arrival. Depending on the extend of the project a final report will be delivered within 6 weeks from start of the analysis. (Note: Timing is a guide only. please contact us for a customized planification).

Deliverables:

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

https://www.solveresearch.com/techniques/auc/

Electrical FFF (EAF4) is a new analytical technique. 

Applicability: Electrical FFF (EAF4) is a method applicable to exosomes, drug delivery vehicles, proteins. Typical uses of EAF4 is separation of proteins based on size and simultaneous determination of electrical characteristics of each population (zeta-potential and effective net charge over the size distribution.

Turnaround time: the analysis will start within 2 weeks from sample arrival. Depending on the extend of the project a final report will be delivered within 6 weeks from start of the analysis. (Note: Timing is a guide only. please contact us for a customized planification).

Deliverables: 

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

• A written report of the results.
• Telephone meetings for discussions of analysis and results.

https://www.solveresearch.com/techniques/dls/

- Instrumentation: Mastersizer Malvern

- Scanning electron microscopy
- Transmission electron microscopy

We offer comprehensive characterization of particles/powders (1 µm to 30 mm) with image analysis using QICPIC.

- Instrumentation: Sympatec QICPIC.
- Application: Particle size and shape analysis, aspect ratio, particle gallery. 
- Standard sample requirements: Particles 1 µm to 30 mm.