SkinAxis provides advanced technology for skin research
SkinAxis conducts in vitro efficacy testing of active ingredients and final products using advanced 2D (2Dimensional) & 3D technologies for chemical, pharma and cosmetic companies. Our technology provides more sensitive and accurate identification of activity profile of drugs and active ingredients. 3D skin equivalent system will perform similarly to the current in vivo models used to test cosmetic ingredients for aging, skin barrier function, skin pigmentation, UV protection, DNA damage, antioxidant pathway, skin irritation assay. SkinAxis offers unique QuantiSig technology for evaluation of different pathways in 2D or 3D skin
3D skin models for detection of UV damage
SkinAxis generates 3D skin models for the highly sensitive detection of UV damage using genetically engineered skin cells. Each cell type is engineered to allow the expression a reporter gene upon activation of the DNA damage response pathway. As UV irradiation causes DNA damage, these cells are particularly suitable to assess the effects of UV on skin in a quantifiable manner. Engineered cells are able to reconstitute the normal architecture of skin tissue after establishing air-liquid interface and cultivation in differentiation media. Novel 3D skin models allow rapid and quantitative assessment of the effects of UVA and UVB irradiation.
Product Description
Human macrophages differentiated from Peripheral Blood Mononuclear Cells (PBMC)-derived monocytes.
Sample Collection and Processing
CD14+ monocytes were isolated from buffy coats of normal human volunteers by positive selection using an
immunomagnetic cell separation method. Monocytes were differentiated into macrophages by culturing cells for 10-13 days in the SkinAxis proprietary medium.
Purity
The purity of PBMC-derived CD14+ monocytes, as measured by flow cytometric analysis, is ≥95% (left panel).
Macrophages are >99% positive for the pan-macrophage CD68 marker and show efficient phagocytic function.
Thawing and General Culture Protocol
Stability and Storage
Macrophages are cryopreserved in the SkinAxis Freezing Medium and are stable for long-term storage at -152o C or in liquid nitrogen. Storage at -80o C is NOT recommended. Once thawed, the cells must be used immediately.
Quality control
Cells are derived from donors with undetectable HIV-1, hepatitis B and hepatitis C. Macrophages are characterized by morphological analysis, specific marker expression, and phagocytic ability.
Warning
Primary cells from human blood are a potential biohazard. Treat as potentially infectious. All human sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products.
For more information, please see the Macrophage Data Sheet sheet in the Resources section on our Storefront (right hand side).
2D genetically engineered skin cell models
SkinAxis provides intact and genetically engineered primary human skin models including epidermal keratinocytes, melanocytes, and dermal fibroblasts. Each cell type has been engineered to carry a stable transgene that allows the expression of the reporter upon activation of specific pathways. For testing of active ingredients, we follow the cellular response and reporter gene in cell-specific culture medium.
3D skin models
SkinAxis established a wide range of in vitro 3D skin models. We provide 3D models developed using different types of skin cells. SkinAxis’ novel approach is based on the co-culture of multiple human primary skin cells in three-dimensional (3D) systems in physiologically relevant conditions in which skin’s native biology can be reconstituted and analyzed. We establish air-liquid interface in 3D models to generate stratification and differentiation of skin cells.
These models can be used to identify and validate active ingredients or final products.
Genetically engineered 3D skin models
SkinAxis generates genetically engineered 3D skin models following the transduction of primary skin cells with viral vectors carrying reporter genes under the control of tissue specific promoters. SkinAxis has developed a series of vectors and cell lines for the expression of fluorescent as well as chemiluminescent reporter proteins. The engineered cells maintain their physiological responses while allowing the precise assessment and more detailed analysis of the regulation of skin molecular targets upon treatment with drugs and active ingredients.
3D skin models for detection of UV damage
SkinAxis generates 3D skin models for the highly sensitive detection of UV damage using genetically engineered skin cells. Each cell type is engineered to allow the expression a reporter gene upon activation of the DNA damage response pathway. As UV irradiation causes DNA damage, these cells are particularly suitable to assess the effects of UV on skin in a quantifiable manner. Engineered cells are able to reconstitute the normal architecture of skin tissue after establishing air-liquid interface and cultivation in differentiation media. Novel 3D skin models allow rapid and quantitative assessment of the effects of UVA and UVB irradiation.
Normal Peripheral Blood Monocyte Derived Macrophages (MDM) M1 and M2
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