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Sino Biological

1 Order Completed
Wayne, Pennsylvania, US

About Sino Biological

Founded: 2007 Type: Privately Held Size: 201-500 employees

**Sino Biological offers services and/or reagents specific to COVID-19 research (see below). Submit a quote request containing the word ‘COVID-19’ to receive even more details.**

Founded by MIT professor, Dr. Daniel I.C. Wang, and his former student, Dr. Liangzhi...

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**Sino Biological offers services and/or reagents specific to COVID-19 research (see below). Submit a quote request containing the word ‘COVID-19’ to receive even more details.**

Founded by MIT professor, Dr. Daniel I.C. Wang, and his former student, Dr. Liangzhi Xie, in 2007, Sino Biological Inc. is a developing biological reagents manufacturer located in Beijing, offering a comprehensive set of premium quality reagents, all of which are produced in-house and cover a broad range of life science research and drug development. It also provides one-stop services for protein and antibody discovery, research, development, production and commercialization. Meanwhile, Sino Biological is dedicated to research and development of biological pharmaceuticals. Sino Biological provides biological research reagents and CRO services to the global life science and biotech community. As a world-leading manufacturer of biological products, we offer a one-Stop Biology Services for protein and antibody discovery, research, development, production, and commercialization. >400 recombinant monoclonal antibody production projects (10mg to 30 gram) for our customers worldwide in the past two years, More than 100 of these were intended for pre-clinical in vivo studies >600 recombinant proteins produced at bulk quantities in two years

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Our Services (23)


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Virus Production

Price on request

CoV spike

 

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on... Show more »

CoV spike

 

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV. 

All CoV spike Reagents:

CoV spike proteins, recombinant: There are 12 CoV spike protein produced in house with high quality which are covering various species. All these CoV spike protein are expressed by different host cells.  These CoV spike proteins are produced with different tags, such as His Tag, Fc Tag. 
Anti-CoV spike Antibody: There are 23 CoV spike antibodies which are validated in multiple tissues with various applications, including WB, ICC/IF, IP, IHC-P, ELISA, FCM, Microneutralizaiton(MN). All the CoV spike anbodies are produced in house and all are in stock. CoV spike antibody customerized service is available. 
CoV spike Overexpression Lysate: CoV spike overexpression lysate was created in HEK293 Cells, Baculovirus-Insect cells, Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of CoV spike protein from the overexpression lysate was verified. 
CoV spike cDNA Clones: All these CoV spike cDNA clone are full sequence confirmed. There are 12 CoV spike expression cDNA clones with various fusion tags, especially GFPspark tag and OFPspark tag. CoV spike expression cDNA clones are expression validated.CoV spike cDNA clones customerized service are available. 
CoV spike Immunoprecipitation (IP) Kit: The Anti-CoV spike magnetic Beads, conjugated with Anti-CoV spike antibody, are used for immuneprecipitation (IP) ofCoV spike proteins which expressed in vitro expression systems. 

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Large Scale Monoclonal Antibody (mAb) Production

Price on request

Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of... Show more »

Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.

Advantages:

  • Self-developed high-efficiency culture medium and transfection reagents, high-expression vectors and engineering cell lines with indigenous intellectual property, ensuring high success rates at low cost for antibody production.
  • Different culture scale from shake flasks to bioreactors, 24 hours a day, 7 days a week continuous culturing to ensure rapid production of antibodies.
  • Provide transient transfection for antibody production, and develops stable cell lines for antibodies production to meet different customers needs.
  • Express full-length antibody of each subtype (IgG1, IgG2a, IgG2b, IgG3, IgG4, IgM, etc.), chimeric antibody (human/mouse chimeric antibody, mouse/rabbit chimeric antibody, etc.), and also antibody fragments (Fab, scFv, nanobody, etc.).
  • Produce antibodies with high-purity, high-concentration and low-endotoxin according to customers demands.
  • One-stop Service: Mab development- Hybridoma antibody gene sequencing - Mouse antibody production - Chimeric antibody production - Antibody humanization - Antibody affinity maturation - Antibody characterization and analysis- Process development - GMP production.

Delivery: • Purified antibody • SDS-PAGE data • CoA

Turnaround: 

Gene synthesis and codon optimization (optional): 1-2 weeks

Vector construction: 1-2 weeks

Expression & purification: 1-2 weeks

QC analysis: 3days

 

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High Throughput Antibody Expression

Price on request

Sino Biological provides high-throughput recombinant antibody expression and purification services that has combined professional high-throughput gene synthesis, vector construction, and optimized technology of transient antibody expression. Making full use of superior mammalian cell expression platforms, Sino Biological can... Show more »

Sino Biological provides high-throughput recombinant antibody expression and purification services that has combined professional high-throughput gene synthesis, vector construction, and optimized technology of transient antibody expression. Making full use of superior mammalian cell expression platforms, Sino Biological can provide services of rapid production of recombinant antibodies in HEK293/CHO cells.

Advantages:

Free codon optimization, Optimized high-efficiency expression vectors, Optimized culture medium and expression systems, Flexible production scales, Fast turnaround, Great expression capacity: >100 antibodies per week 

Delivery: • Purified antibody • CoA • Expression vector (Optional)

Turnaround: 2-4 weeks

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Biology High-throughput Recombinant antibody HEK293 CHO

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Recombinant Antibody Production

Price on request

Sino Biological has a professional mammalian cell expression platform with more than 10 years of process optimization and production experience, providing one-stop services from gene synthesis to high-quality antibody production. 

Items:

  • High-throughput Antibody Production Service... Show more »

Sino Biological has a professional mammalian cell expression platform with more than 10 years of process optimization and production experience, providing one-stop services from gene synthesis to high-quality antibody production. 

Items:

  • High-throughput Antibody Production Service (0.1mg-1mg)
  • Large-scale Antibody Production Service (100mg-100g)
  • Chimeric Antibody Production Service
  • Antibody Fragment Production Service
  • Bispecific Antibody Production Service

Advantages:

  • Capacity and technology platform: Optimized high-efficiency expression vectors, proprietary culture medium and mammalian cell expression platform
  • Rich experience: • Experienced in expressing different kinds of antibodies: scFv antibody, Fab antibody, full length antibody, chimeric antibody, bispecific antibody, multi-chains co-transfection and expression, etc. • Experienced in expressing and purifying multi-isotypes antibodies:Human IgG1, IgG2, IgG3, IgG4 ; Mouse IgG1, IgG2a, IgG2b, IgG3; Rat IgG1, IgG2a, IgG2b, IgG2c ; Rabbit IgG; Canine IgG; IgA, IgM, IgE
  • High quality: High monomer purity: up to 99%, Low endotoxin level: as low as 0.1 EU/mg and Comprehensive quality control analysis: UV, SDS-PAGE, ELISA, qPCR, SEC-HPLC, CEX-HPLC, LC-MS/MS, CE-SDS, ADCC,CDC, OCTET Affinity, Kinetics, off-rate, ranking

 

>High-throughput Antibody Production Service:

 

Sino Biological provides high-throughput recombinant antibody expression and purification services that has combined professional high-throughput gene synthesis, vector construction, and optimized technology of transient antibody expression. Making full use of superior mammalian cell expression platforms, Sino Biological can provide services of rapid production of recombinant antibodies in HEK293/CHO cells.

Advantages:

Free codon optimization, Optimized high-efficiency expression vectors, Optimized culture medium and expression systems, Flexible production scales, Fast turnaround, Great expression capacity: >100 antibodies per week 

Delivery: • Purified antibody • CoA • Expression vector (Optional)

Turnaround: 2-4 weeks

 

>Large-scale Antibody Production Service

 

Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. Sino Biological can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.

Advantages:

  • Self-developed high-efficiency culture medium and transfection reagents, high-expression vectors and engineering cell lines with indigenous intellectual property, ensuring high success rates at low cost for antibody production.
  • Different culture scale from shake flasks to bioreactors, 24 hours a day, 7 days a week continuous culturing to ensure rapid production of antibodies.
  • Provide transient transfection for antibody production, and develops stable cell lines for antibodies production to meet different customers needs.
  • Express full-length antibody of each subtype (IgG1, IgG2a, IgG2b, IgG3, IgG4, IgM, etc.), chimeric antibody (human/mouse chimeric antibody, mouse/rabbit chimeric antibody, etc.), and also antibody fragments (Fab, scFv, nanobody, etc.).
  • Produce antibodies with high-purity, high-concentration and low-endotoxin according to customers demands.
  • One-stop Service: Mab development- Hybridoma antibody gene sequencing - Mouse antibody production - Chimeric antibody production - Antibody humanization - Antibody affinity maturation - Antibody characterization and analysis- Process development - GMP production.

Delivery: • Purified antibody • SDS-PAGE data • CoA

Turnaround: 

Gene synthesis and codon optimization (optional): 1-2 weeks

Vector construction: 1-2 weeks

Expression & purification: 1-2 weeks

QC analysis: 3days

 

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Phospho-Specific Antibody Production

Price on request

Post-translational modification, especially phosphorylation of proteins critical for the biological function of proteins. It plays an important role in many key regulatory mechanisms of life phenomena such as signaling, cell cycle, proliferation, metabolism, growth & development, and cancer... Show more »

Post-translational modification, especially phosphorylation of proteins critical for the biological function of proteins. It plays an important role in many key regulatory mechanisms of life phenomena such as signaling, cell cycle, proliferation, metabolism, growth & development, and cancer mechanisms.

Phosphorylation process is usually the addition of a phosphate (PO4) group to a protein, commonly at serine (S), threonine (T) or tyrosine (Y) residue via the action of a kinase. Phosphorylation-Specific Antibody can discriminate between the phosphorylated and the non-phosphorylated state of a protein. It is one of the most frequently asked for types of PTM specific antibodies.

Applications:

  • - Protein phosphorylation levels are often associated with protein activity: Many signaling pathway proteins cause a series of cellular responses by altering their phosphorylation levels after some stimulation, but their protein content does not change much.
  • - Phospho-specific antibody: Prepared against a specific phosphorylation site to detect the changes in the functional state of the protein (enhanced or decreased phosphorylation level) when the cell is stimulated.
  • - Non-phospho-specific antibody: It mainly detects the protein expression level, tissue distribution or cell localization in tissues or cells, and can also be used as an internal reference for changes in phosphorylation level.

Items & Procedures

- Design and synthesis of phosphopeptide - Animal immunization - Monoclonal antibody screening & library construction - Antibody production and purification - Quality verification & delivery

Technical Platforms:

  • Phospho-Specific Rabbit Pab Production Platform: Short cycle, low price
  • Phospho-Specific Mouse Mab Production Platform: High specificity and good lot-to-lot consistency
  • Phospho-Specific Rabbit Mab Production Platform: High affinity and sensitivity
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Antibodies

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Protein Production

Price on request

Sino Biological Inc. has over 10 years' experience with 6000+ recombinant proteins production. And there is a complete system of protein expression, from gene sequence to purified protein. We have multiple protein expression systems, such as bacterial, yeast, baculovirus-insect and mammalian expression system with 30+... Show more »

Sino Biological Inc. has over 10 years' experience with 6000+ recombinant proteins production. And there is a complete system of protein expression, from gene sequence to purified protein. We have multiple protein expression systems, such as bacterial, yeast, baculovirus-insect and mammalian expression system with 30+ purification systems to choose. High R & D ability with over 1000 new proteins per year, quick problem-solving ability, and close to 100 bioreactors with a variable volume between 2-1000 L to guarantee high-throughput and large-scale production allow us to serve scientists around the world.

Advantages

  • - High-efficiency expression vectors
  • - High cell density culturing - Proprietary medium formulation
  • - Over 6000 proteins expression and purification experience
  • - Inclusion body refolding & soluble expression
  • - Protein biopharmaceutical technology

Items

  • - E.coli protein expression service
  • - Baculovirus-insect expression service
  • - Mammalian transient protein expression service
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Antibody Services

Price on request

Sino Biological has more than 10 years experience in antibody development and production, and is committed to providing scientific research institutions and pharmaceutical companies with one-stop services including antigen production, antibody development, ELISA kit development, antibody detection and production, hybridoma... Show more »

Sino Biological has more than 10 years experience in antibody development and production, and is committed to providing scientific research institutions and pharmaceutical companies with one-stop services including antigen production, antibody development, ELISA kit development, antibody detection and production, hybridoma antibody gene sequencing, chimeric antibody and antibody fragment expression, antibody humanization, antibody affinity maturation, anti-idiotype antibody and bispecific antibody production services.

Advantages

  • - Varied expression platforms for antigen: Five antigen expression platforms including HEK293, CHO, E.coli, Yeast,and Insect cell can meet the needs of different types of antigen recombinant expression.
  • - Multiple antigen types:Polypeptide and protein antigens are two main methods for immunization and screening, various methods such as DNA, cell and insect virus immunization are also available
  • - Various animal species for immnization:Mouse and rabbit Different antibody
  • - development technologies:Hybridoma technology and antibody library screening technology can be used to increase the probability of obtaining positive clones and high-specificity & high-affinity antibodies
  • - Diversified antibody screening technologies:Solid-phase and liquid-phase antibody screening techniques can both be used to reduce the amount of coating antigen and increase antibody diversity
  • - Multiple antibody detection platforms: ELISA, WB, IHC, FACS, SEC-HPLC, Octet, endotoxin, mass spectrometry and other antibody detection platforms can be used for comprehensive assessment of antibody quality
  • - Various antibody purification methods:Protein A, protein G, protein L, size exclusion chromatography, ion exchange chromatography, antigen affinity purification and other purification methods will be done to improve antibody purity
  • - Antibody recombinant expression technology:Using optimized plasmids and HEK293 cell line, Sino Biological enables the rapid, high-throughput and large-scale production of recombinant antibodies and antibody fragments
  • - Hybridoma culture technology:Serum-free suspension culturing of hybridoma cells can be used, compared with ascites, the antibody will not include complex components and will avoid the serum influence on detection

Items

  • - Antibody Production & Development Services
  • - Mini-scale Antibody Production Service(0.1mg-1mg)
  • - Large-scale Antibody Production Service(100mg-100g)
  • - Recombinant Antibody Production Service
  • - Rabbit Mab Production Service
  • - Standard Mouse Mab Production Service
  • - Speedy Mouse Mab Production Service
  • - Standard Rabbit Pab Production Service
  • - Speedy Rabbit Pab Production Service
  • - Phospho-Specific Antibody Production Service
  • - Antigen production Service
  • - ELISA Kit development Service
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Antibody Products

Price on request

Sino Biological has a professional mammalian cell expression platform with more than 10 years of process optimization and production experience, providing one-stop services from gene synthesis to high-quality antibody production. For the past 10 years, Sino Biological has provided thousands of antibodies production service to... Show more »

Sino Biological has a professional mammalian cell expression platform with more than 10 years of process optimization and production experience, providing one-stop services from gene synthesis to high-quality antibody production. For the past 10 years, Sino Biological has provided thousands of antibodies production service to customers worldwide and has established good cooperation relationships with world's leading pharmaceutical companies. Sino Biological is the preferred antibody production service provider for Top10 pharmaceutical companies worldwide.

Advantages

  • Capacity and technology platform
  • Rich experience
  • High quality

Items

  • Mini-scale Antibody Production Service (0.1mg - 1mg)
  • Small and Mid-scale Antibody Production Service (1mg- 100mg)
  • Large-scale Antibody Production Service (100mg - 100g)
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Recombinant Protein Expression

Price on request

Sino Biological Inc. has over 10 years' experience with 6000+ recombinant proteins production. And there is a complete system of protein expression, from gene sequence to purified protein. We have multiple protein expression systems, such as bacterial, yeast, baculovirus-insect and mammalian expression system with 30+... Show more »

Sino Biological Inc. has over 10 years' experience with 6000+ recombinant proteins production. And there is a complete system of protein expression, from gene sequence to purified protein. We have multiple protein expression systems, such as bacterial, yeast, baculovirus-insect and mammalian expression system with 30+ purification systems to choose. High R & D ability with over 1000 new proteins per year, quick problem-solving ability, and close to 100 bioreactors with a variable volume between 2-1000 L to guarantee high-throughput and large-scale production allow us to serve scientists around the world.

Advantages

  • - High-efficiency expression vectors
  • - Multiple expression vectors and E.coli cells to meet various expression needs
  • - High cell density culturing
  • - Proprietary medium formulation
  • - Over 10 years of 6000 proteins expression and purification experience
  • - Inclusion body refolding & soluble expression
  • - Protein biopharmaceutical technology

Items 

  • - E.coli expression service
  • - Mammalian transient protein expression service
  • - Baculovirus-insect expression service
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Monoclonal Antibody (mAb) Production

Price on request

Advantages:

  • - Advanced technologies: • Optimized immunization • High efficient electrofusion • Phage display library technology • Solid / Liquid-phage screening • Hybridoma serum-free cell culture • Multiple purification methods
  • - Various antigens:... Show more »

Advantages:

  • - Advanced technologies: • Optimized immunization • High efficient electrofusion • Phage display library technology • Solid / Liquid-phage screening • Hybridoma serum-free cell culture • Multiple purification methods
  • - Various antigens: • Recombinant protein • Natural protein • Virus • Peptide • Cell • DNA
  • - Customized service:  • Optional validation: ELISA, WB, IHC, IF, FACS, ICC, Octet, MS, etc. • Multiple screening methods at key nodes • Milestone payments • Timely feedback and communication

Items

  • - Rabbit mAb Production Service
  • - Standard Mouse mAb Production Service
  • - Fast Mouse mAb Production Service
  • - Recombinant Antibody Production Services

 

﹥Rabbit mAb Production Service

 

Sino Biological develops rabbit mAb by constructing antibody library to directly obtain antibody genes and their sequences, which is more suitable for the long-term preservation of excellent antibodies and their recombinant expression.

Delivery: - Antibody H and L chains sequences - 0.1-0.5mg purified mAb - Vectors containing the H and L chain, respectively - Experimental report

Turnaround: - Antigen validation: 1-2 days - Immunization and serum titer test: 8-10 weeks - Library construction & screening: 4-6 weeks - Antibody production & purification: 2-3 weeks - QC analysis: 3days

Guarantee ELISA positive for immunogen

 

﹥Standard Mouse mAb Production Service

 

Mouse mAb developed by Sino Biological has the advantages of high sensitivity & specificity. We can also provide flexible customized solutions according to customer requirements.

Delivery: - 2-5 positive clones (2 tubes of cryopreserved cells) - 2-5mL supernatant per clone - 1-3mg purified antibody (choose a hybridoma cell) - Experimental report

Turnaround: - Antigen validation: 1-2 days - Immunization and serum titer test: 8-10 weeks - Fusion and Screening: 4-6 weeks - Antibody production & purification: 2-3 weeks - QC analysis: 3days

Guarantee ELISA positive for immunogen

 

﹥Fast Mouse mAb Production Service

 

Sino Biological Inc. has shortened the immunization time to 4-5 weeks and launched Speedy Mouse mAb Production Service, which can offer high-quality antibodies more quickly and efficiently.

Advantage:

  • Short production time for antibodies
  • Without increasing the dose of immunogens
  • High fusion efficiency with electrofusion technique
  • Able to produce antibodies for a specific application such as FC, IHC, and WB
  • Rapid production scale from milligram to gram level
  • Antibody with high purity, high lot-to-lot reproducibility and without animal viruses contamination

Turnaround: - Antigen Preparation - Immunization and serum titer test: 5 weeks - Fusion and screening: 5 weeks - Antibody production and analysis: 3 weeks

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Monoclonal Antibody (mAb) Development

Price on request

Rabbit mAb Development Service

Sino Biological develops rabbit mAb by constructing an antibody library to directly obtain antibody genes and their sequences, which is more suitable for the long-term preservation of excellent antibodies and their recombinant expression.

Advantages

Rabbit mAb Development Service

Sino Biological develops rabbit mAb by constructing an antibody library to directly obtain antibody genes and their sequences, which is more suitable for the long-term preservation of excellent antibodies and their recombinant expression.

Advantages

  • - International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience in 6000+ protein production, a high success rate of antigen expression
  • - Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of the designed peptide is greater than 95%
  • - Leading antibody library construction and screening technology, reduce the risk of antibody fragment deletions or mismatches and increase the probability of screening for target antibodies
  • - ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
  • - High affinity, 10-100 times more than mouse mAb, in average
  • - One-stop service: From antibody development, vector construction to antibody expression & purification, saving customers time
  • - Quoting by steps, charging by nodes, reducing customer's risk and saving costs

Delivery: - Antibody H and L chains sequences for 1 positive clone - 0.1-0.5mg purified mAb - Vectors containing the H and L chain, respectively -Experimental report 

Turnaround: - Antigen validation: 1-2 days - Immunization and serum titer test: 8-10 weeks - Library construction & screening: 4-6 weeks - Antibody production & purification: 2-3 weeks - QC analysis: 3days

Guarantee ELISA positive for immunogen

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Polyclonal Antibody Production

Price on request

Sino Biological offers cost-effective polyclonal antibody production services, which involve peptide or protein antigen synthesis, rabbit or goat immunization, antibody purification and QC control test.

Sino Biological has rich experience in custom polyclonal antibodies and guarantees an ELISA titer of 1:64,000. We've... Show more »

Sino Biological offers cost-effective polyclonal antibody production services, which involve peptide or protein antigen synthesis, rabbit or goat immunization, antibody purification and QC control test.

Sino Biological has rich experience in custom polyclonal antibodies and guarantees an ELISA titer of 1:64,000. We've designed multiple service packages that can fit into various customer's needs. WB/IHC guaranteed pAb services guarantee positive binding to antigen in ELISA or WB/IHC assay. Fast rabbit pAb service delivers custom polyclonal antibodies from animal immunization to purified antibodies in just 45 days.

Items:

  • Standard Rabbit pAb Production
  • Fast Rabbit pAb Production
  • Phospho-Specific Antibody Production

 

>Standard Rabbit pAb Production

 

Rabbit pAb has been an important tool for scientific researchers because of its advantages. Sino Biological has both excellent antigen design and antibody development team, and rich experience in rabbit PAb development. We aim to develop high-quality rabbit pAb that meets customers' application requirements.

Advantages

  • - International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience in 6000+ protein production, a high success rate of antigen expression
  • - Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of the designed peptide is greater than 95%
  • - Serum titer ≥1:64,000
  • - Quoting by steps, charging by nodes, reducing customer's risk and saving costs

Delivery

- Purified pAbs from all 30-40ml serum/rabbit - Experimental report

Turnaround

- Antigen validation: 1-2 days - Immunization and serum titer test: 8-10 weeks - Purification: 3 days - QC analysis: 3days Guarantee ELISA titer: ≥1:64,000

 

>Fast Rabbit pAb Production

 

 Sino Biological Inc. has launched Speedy Rabbit Polyclonal Antibody Production Service which can complete polyclonal antibody production and identification within 45 days, and provide high-quality antibodies more quickly and efficiently.

Advantage

  • World-leading recombinant protein expression and purification techniques, professional peptide design and high-efficiency conjugation techniques
  • Short production time, completing antibody production within 45 days
  • Guaranteed Rabbit pAb with a serum ELISA titer of ≥1:64,000
  • Antibody with high sensitivity, using less antibody each time
  • Guaranteed delivery amount, meeting experiment needs
  • Quoting by steps, charging by nodes, reducing customer's risk and saving costs

Turnaround

Antigen Preparation, Immunization and serum titer test: 5-6 weeks, Purification (Optional): 3 days, QC analysis: 3 days

 

>Phospho-Specific Antibody Production

 

Post-translational modification, especially phosphorylation of proteins critical for the biological function of proteins. It plays an important role in many key regulatory mechanisms of life phenomena such as signaling, cell cycle, proliferation, metabolism, growth & development, and cancer mechanisms.

Phosphorylation process is usually the addition of a phosphate (PO4) group to a protein, commonly at serine (S), threonine (T) or tyrosine (Y) residue via the action of a kinase. Phosphorylation-Specific Antibody can discriminate between the phosphorylated and the non-phosphorylated state of a protein. It is one of the most frequently asked for types of PTM specific antibodies.

Applications:

  • - Protein phosphorylation levels are often associated with protein activity: Many signaling pathway proteins cause a series of cellular responses by altering their phosphorylation levels after some stimulation, but their protein content does not change much.
  • - Phospho-specific antibody: Prepared against a specific phosphorylation site to detect the changes in the functional state of the protein (enhanced or decreased phosphorylation level) when the cell is stimulated.
  • - Non-phospho-specific antibody: It mainly detects the protein expression level, tissue distribution or cell localization in tissues or cells, and can also be used as an internal reference for changes in phosphorylation level.

Procedures

- Design and synthesis of phosphopeptide - Animal immunization - Monoclonal antibody screening & library construction - Antibody production and purification - Quality verification & delivery

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Bacterial Protein Expression

Price on request

E.coli Protein Expression Service

Sino Biological has abundant experience of soluble protein expression and purification and protein refolding, and multiple E.coli cell lines and expression vectors. E.coli cell possesses features of clear genetic background, high expression of target genes, short cycle,... Show more »

E.coli Protein Expression Service

Sino Biological has abundant experience of soluble protein expression and purification and protein refolding, and multiple E.coli cell lines and expression vectors. E.coli cell possesses features of clear genetic background, high expression of target genes, short cycle, strong anti-pollution ability and easy to be scaled up. E.coli protein expression system is suitable for protein expression of multiple species, acting as a principal tool for recombinant protein expression and biotechnological industrial development, especially for small protein production.

Advantages

  • - Over 10 years' experience of protein expression
  • - Multiple protein purification and refolding technologies
  • - High-throughput and large-scale protein expression and production
  • - One-stop service from gene to purified protein
  • - Multiple expression vectors and E.coli cells to meet various expression needs

Delivery:

- Sequencing report - Expression evaluation result - Feasibility report - If expression is feasible, provide a certain amount of protein sample to the client based on the expression - Purified protein - SDS-PAGE report - CoA

Turnaround:

- Gene synthesis and codon optimization (optional): 1-2 weeks - Vector construction: 1-2 weeks - Expression and purification: 1-2 weeks - Large-scale expression and purification (optional): 2-3 weeks

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Biopharmaceutical Development

Price on request

Sino Biological has a comprehensive antibody drug research and development platform, with experienced scientists and advanced equipment to provide monoclonal antibody preparation, high-throughput antibody production, antibody engineering, and biopharmaceutical analysis services involving physicochemical analysis and biological... Show more »

Sino Biological has a comprehensive antibody drug research and development platform, with experienced scientists and advanced equipment to provide monoclonal antibody preparation, high-throughput antibody production, antibody engineering, and biopharmaceutical analysis services involving physicochemical analysis and biological activities.

Advantages

  • ﹥6,000 protein expression experience 
  • ﹥20,000 antibody screening experience
  • Successfully screened dozens of candidate antibodies with good biological activity and druggability against several important drug targets 
  • Established cell activity evaluation models for many antibodies against key drug targets (PD-1, PD-L1, and EGFR, etc)
  •  A full set of preclinical research experience for multiple key drug targets

Items

- Biopharmaceutical Analysis Service

- Recombinant Antibody Production Service

 

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Transient Protein Expression

Price on request

Sino Biological mammalian transient expression system, mainly using CHO cells and HEK293 cells as expression hosts, can provide flexible services which could meet different customer needs, including tag removal, endotoxin removal, SEC-HPLC assay, glycosylation analysis and so... Show more »

Sino Biological mammalian transient expression system, mainly using CHO cells and HEK293 cells as expression hosts, can provide flexible services which could meet different customer needs, including tag removal, endotoxin removal, SEC-HPLC assay, glycosylation analysis and so on.

Advantages

  • High-efficiency expression vectors 
  • Over 10 years' experience in protein expression 
  • Optimized mammalian expression systems 
  • One-stop service from gene sequence to purified protein
  • High-throughput and large-scale production 
  • High yield expression with post-translational modification and correct folding

Delivery

- Sequencing report - Feasibility report - 0.1-0.5 mg sample (if the expression is feasible and sample remains from QC testing) - Purified protein -SDS-PAGE report - CoA

Turnaround

- Gene synthesis and codon optimization (optional): 1-2 weeks - Vector construction: 1-2 weeks - Expression and purification: 2-3 weeks - Large-scale expression and purification (optional): 2-3 weeks

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Baculovirus/Insect Cell Protein Expression

Price on request

Sino Biological possesses abundant experience of baculovirus-Insect cell protein expression, especially the expression of large proteins, toxic proteins, kinases, intracellular proteins and membrane proteins.

Advantages

  • - Multiple insect cells: Sf9, Sf21, Hi-5, etc.
  • - Over 10 years' experience of... Show more »

Sino Biological possesses abundant experience of baculovirus-Insect cell protein expression, especially the expression of large proteins, toxic proteins, kinases, intracellular proteins and membrane proteins.

Advantages

  • - Multiple insect cells: Sf9, Sf21, Hi-5, etc.
  • - Over 10 years' experience of protein expression
  • - One-stop service from gene sequence to purified protein
  • - Optimized expression vectors and higher-titer virus packaging
  • - High-efficiency expression of intracellular and extracellular proteins
  • - High-throughput and large-scale protein production

Delivery

- Sequencing report - Feasibility report - 0.1-0.5 mg sample (if the expression is feasible and sample remains from QC testing) - Purified protein -SDS-PAGE report - CoA  

Turnaround

- Gene synthesis and codon optimization (optional): 1-2 weeks - Vector construction: 1-2 weeks - Virus packaging and amplification: 2-3 weeks - Expression and purification: 2-3 weeks - Large-scale expression and purification (optional): 2-3 weeks

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Biology Proteins milligram Insects Drug Discovery

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Bispecific Antibody Production

Price on request

Sino Biological can provide you with fast and efficient bispecific antibody expression service, based on the optimized mature mammalian cell expression platform. A bispecific antibody is an artificial antibody containing two kinds of specific antigen binding sites and can establish a bridge between target cells and functional... Show more »

Sino Biological can provide you with fast and efficient bispecific antibody expression service, based on the optimized mature mammalian cell expression platform. A bispecific antibody is an artificial antibody containing two kinds of specific antigen binding sites and can establish a bridge between target cells and functional molecules (cells) to stimulate a directed immune response. It is a genetically engineered antibody, which has become a hot spot in the field of antibody engineering and has a broad application prospect in tumor immunotherapy.

Advantages 

  • Optimized HEK293 or CHO mammalian cell expression platforms: better glycosylation and post-translational modifications 
  • Multiple purification methods: Protein A/G/L affinity purification Anion and cation exchange chromatography Size Exclusion Chromatography Column (SEC) 
  • Powerful QC analysis: SEC-HPLC, MS, activity assay, endotoxin test, glycosylation analysis, etc

Deliverables:

- Purified antibody - SDS-PAGE data - CoA

Turnaround

- Gene synthesis and codon optimization (optional): 1-2 weeks - Vector construction: 1-2 weeks - Expression & purification: 1-2 weeks - QC analysis: 3 days

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HPLC-SEC endotoxin Glycosylation Antibodies Drug Discovery

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Anti-Idiotype Antibody Production

Price on request

Sino Biological Inc. has various solutions to problems that you may encounter during the development of anti-idiotype antibodies. We can offer a full range of services - from antigen preparation, anti-idiotypic antibody development to detection method establishment and kit development.

Items:

Sino Biological Inc. has various solutions to problems that you may encounter during the development of anti-idiotype antibodies. We can offer a full range of services - from antigen preparation, anti-idiotypic antibody development to detection method establishment and kit development.

Items:

  • Anti-idiotype Rabbit pAb Production Service
  • Anti-idiotype Mouse mAb Production Service
  • Anti-idiotype Rabbit mAb Production Service

Advantages

  • Rich experience in antigen preparation: antigen expression, full-length antibody and purification service
  • Different kinds of antigens: full-length antibody, F(ab')2
  • Various species: perfect for ELISA kit development
  • A variety of purification methods: Protein A, antibody drug, human IgG, isotype control
  • Multiple immunization strategies: increased titer and higher success rate 
  • Proprietary rabbit monoclonal antibody technology: larger library size and higher affinity
  • High-throughput antibody expression and screening technology

Application:

• Antibody drug immunogenicity studies
• Vaccine development
• Anti-drug antibodies (ADA) for clinical development
• Antibody drug pharmacokinetic/pharmacodynamics (PK/PD) studies: detecting free, total or bound drug in blood

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Antibodies Drug Discovery High-throughput antibody screening

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Antibody Fragment Production

Price on request

Fab/scFv Antibody Fragment Production Services

Sino Biological has developed a technology platform in mammalian cells for the expression and production of antibody Fab fragments. The recombinant antibody Fab fragments produced by our technology is of superior quality than antibody Fab fragments generated from enzyme... Show more »

Fab/scFv Antibody Fragment Production Services

Sino Biological has developed a technology platform in mammalian cells for the expression and production of antibody Fab fragments. The recombinant antibody Fab fragments produced by our technology is of superior quality than antibody Fab fragments generated from enzyme cleavage of full antibodies.

Sino Biological is capable of producing ScFv single chain antibody fragment using either production systems, depending on customer's preference. ScFv (single-chain Fragment variable) antibody expression and production is a routine work to support antibody discovery with an antibody library. ScFv protein can be readily expressed in a microbial expression system such as E. coli. It can also be expressed in mammalian cells through transient transfection.

Advantages:

  • Different expression systems: E.coli, HEK293 cells, CHO cells
  • Multiple tags: His-tag, FLAG-tag, HA-tag, SUMO-tag, etc.
  • Various species: human, mouse, rat, rabbit, canine, rhesus, etc.

Delivery:

  • - Purified antibody
  • - SDS-PAGE data
  • - CoA

Turnaround:

  • - Gene synthesis and codon optimization (optional): 1-2 weeks
  • - Vector construction: 1-2 weeks
  • - Expression & purification: 1-2 weeks
  • - QC analysis: 3 days
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Chimeric Antibody Production

Price on request

Sino Biological has rich experience in chimeric antibody production covering not only human and mouse antibodies, but also rabbit, rat, canine antibodies. Our professional scientists and engineers are at your service to support your antibody chimerization needs.

Advantages

Sino Biological has rich experience in chimeric antibody production covering not only human and mouse antibodies, but also rabbit, rat, canine antibodies. Our professional scientists and engineers are at your service to support your antibody chimerization needs.

Advantages

  • - HEK293 or CHO cells: better glycosylation and post-translational modifications
  • - Multiple species: variable region species: human, mouse, rat, canine, rhesus, chicken, rabbit, camel, etc. constant region species: human, mouse, rat, canine, rhesus, rabbit, etc.
  • - Multiple subtypes: IgG, IgA, IgM, IgE

Delivery:

- Purified antibody - SDS-PAGE data - CoA

Turnaround:

- Gene synthesis and codon optimization (optional): 1-2 weeks - Vector construction: 1-2 weeks - Expression & purification: 1-2 weeks - QC analysis: 3 days

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Biology Drug Discovery HEK293 CHO Human Mouse Rabbit Rat canine Rhesus/Cynomolgus Monkey Chicken Camelid Show 12 more tags Show less

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ELISA Kits

Price on request

Sino Biological can provide development and production of ELISA kits for target molecules in blood and other biological samples that involved in scientific research, clinical and diagnostic research, and drug development processes. 

Sino Biological has advanced R&D technology platform and professional technical... Show more »

Sino Biological can provide development and production of ELISA kits for target molecules in blood and other biological samples that involved in scientific research, clinical and diagnostic research, and drug development processes. 

Sino Biological has advanced R&D technology platform and professional technical team and has accumulated rich experience in protein expression and purification, antibody development and production, as well as various immunization detection.

Advantages

  • Abundant resources: We have 5 major protein expression and production platforms including HEK293, CHO, E.coli, Yeast, and the Insect cell. As well as optimized reagent formulation, enzyme labeling technology, and production process. Sino Biological can also customize related products and carry out kit development according to customers' need in the least time.
  • Professional R&D platform:Sino Biological has developed more than 100,000 ELISA antibody in the past 10 years. We have successfully developed a 400+ ELISA pair set and 150+ ELISA kits. Sino Biological also has rich experience on ELISA kit customization for dozens of biopharmaceutical companies. 
  • Professional technical support: Our technical support team will provide a suitable proposal to meet customers' need fastly and efficiently. 
  • Quality assurance: We have various natural samples, as well as testing experience which can be used for comprehensive and accurate methodological validation of Elisa kits. 
  • Convenient and fast: We can provide one-stop service with fewer timelines and high efficiency, which can help the customer save time.

Delivery:

- ELISA Kit - CoA

Turnaround: 6-12 weeks

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Antigen Expression and Purification

Price on request

Recombinant Protein & peptide Antigen Production Service

Our antibody development services can accept antigens provided by our customers and can also help customers produce antigens. Sino Biological can provide high-quality peptide antigens and protein antigens. Protein antigens have integrity confirmation. In... Show more »

Recombinant Protein & peptide Antigen Production Service

Our antibody development services can accept antigens provided by our customers and can also help customers produce antigens. Sino Biological can provide high-quality peptide antigens and protein antigens. Protein antigens have integrity confirmation. In addition, Sino Biological can also provide diversified immunization methods such as DNA, cells, and insect viruses to solve the protein expression problems and immunogens are closer to natural proteins.

When the protein in the sample is in its natural conformation, it is preferred to immunize with a protein antigen, such as the development of neutralizing antibodies. If it is difficult to express the proteins or other specific detection requirements is needed, peptide antigens are economical and practical choices, such as the species-specific detection of the same protein.

Advantages

  • International leading recombinant protein production and purification technology, with five antigen expression platforms: HEK293, CHO, E.coli, Yeast, and Insect cell, and multiple protein purification platforms such as nickel column, conjugation receptor, conjugation antibody, protein A/G, size exclusion chromatography, hydrophobic interaction chromatography and ion exchange chromatography etc.
  • With the intellectual property of the medium, highly efficient expression vector and high-density cell culture systems to fully improve expression success rates and save customers cost
  • The leading technical personnel and laboratory equipment, rich experience in 6000+ protein expression and purification, supporting high-throughput, large-scale and rapid production
  • Professional polypeptide design software, experienced designers, and efficient conjugation solutions to ensure the immunization successful rate of the designed polypeptide is greater than 95%

Delivery

- Polypeptide sequences - 2mg unconjugated polypeptide

Turnaround

Polypeptide antigen production: 4-6 weeks

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Glycosylation Analysis

Price on request

Biopharmaceutical Analysis Service, Bioanalytical Services

Sino Biological has highly efficient and standardized enterprise processes and is a leading biotech company focusing on early drug discovery and development services. Sino Biological has passed the ISO9001:2008 quality management system certification and can... Show more »

Biopharmaceutical Analysis Service, Bioanalytical Services

Sino Biological has highly efficient and standardized enterprise processes and is a leading biotech company focusing on early drug discovery and development services. Sino Biological has passed the ISO9001:2008 quality management system certification and can provide supreme analysis services covering biological, physical, biochemical and other properties. With great expertise in biopharmaceutical analysis, we now can provide you rapidly analysis services including protein purity, molecular weight, peptide mapping, charge heterogeneity, glycosylation, thermostability, ADCC, CDC, affinity, residual host cell protein and processes related impurities, etc.

Sino Biological is equipped with LC-MS, GC-HPLC, Fluorescence Spectroscopy, Thermal Stability Analyzer, Dynamic Light Scattering, Capillary Electrophoresis, Octet Biomolecular Interaction Analysis and other advanced instruments. With rigorous scientific attitude and strict and standardized management system, Sino Biological can provide strong support for your scientific research and biopharmaceutical research work.

Advanced Equipment and Platforms for Biological Analysis:

- LC-MS - High-performance liquid chromatography (HPLC) - Capillary isoelectric focusing electrophoresis (CIEF) - Capillary Electrophoresis (CE) - Octet biomolecular interaction analysis - Thermal stability analysis Analysis

Types:

Anti-Idiotype antibody development, Affinity measurement, FcRn binding assays

Items:

- Quantitation - Purity test - Size heterogeneity analysis; Molecular weight analysis; Isoelectric point test - Peptide mapping; N-terminal Sequencing; C-terminal Sequencing; Disulfide Bond Analysis; Sequence coverage; Post-translational modification analysis; Structure analysis; Charge Heterogeneity Analysis; Iso-Asp analysis - Residual DNA Analysis; Host Cell Protein Analysis; Residual Protein A Analysis - Endotoxin Test; Microbial Limits Testing - Thermostability Measurement - Activity Assay

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UV/Vis Spectrophotometry BCA ELISA SDS-PAGE HPLC-SEC LC-MS CE-SDS RP-HPLC Circular Dichroism Fluorescence CEX-HPLC qPCR LAL assay assay optimization Octet CDC cellular activity assay enzyme assay Membrane Filtration Drug Discovery Large molecules/Biologics Show 21 more tags Show less

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