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Sino Biological

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Wayne, Pennsylvania, US

About Sino Biological

Founded: 2007 Type: Privately Held Size: 201-500 employees

Founded by MIT professor, Dr. Daniel I.C. Wang, and his former student, Dr. Liangzhi Xie, in 2007, Sino Biological Inc. is a developing biological reagents manufacturer located in Beijing, offering a comprehensive set of premium quality reagents, all of which are produced in-house and cover a broad range of... Show more »

Founded by MIT professor, Dr. Daniel I.C. Wang, and his former student, Dr. Liangzhi Xie, in 2007, Sino Biological Inc. is a developing biological reagents manufacturer located in Beijing, offering a comprehensive set of premium quality reagents, all of which are produced in-house and cover a broad range of life science research and drug development. It also provides one-stop services for protein and antibody discovery, research, development, production and commercialization.

Meanwhile, Sino Biological is dedicated to research and development of biological pharmaceuticals. Sino Biological provides biological research reagents and CRO services to the global life science and biotech community. As a world-leading manufacturer of biological products, we offer a one-Stop Biology Services for protein and antibody discovery, research, development, production, and commercialization.
>400 recombinant monoclonal antibody production projects (10mg to 30 gram) for our customers worldwide in the past two years, More than 100 of these were intended for pre-clinical in vivo studies
>600 recombinant proteins produced at bulk quantities in two years

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Our Services (25)


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Protein Production

Price on request

Sino Biological Inc. has over 10 years' experience with 6000+ recombinant proteins production. And there is a complete system of protein expression, from gene sequence to purified protein. We have multiple protein expression systems, such as bacterial, yeast, baculovirus-insect and mammalian expression system with 30+ purification... Show more »

Sino Biological Inc. has over 10 years' experience with 6000+ recombinant proteins production. And there is a complete system of protein expression, from gene sequence to purified protein. We have multiple protein expression systems, such as bacterial, yeast, baculovirus-insect and mammalian expression system with 30+ purification systems to choose. High R & D ability with over 1000 new proteins per year, quick problem-solving ability, and close to 100 bioreactors with a variable volume between 2-1000 L to guarantee high-throughput and large-scale production allow us to serve scientists around the world.

Advantages
- High-efficiency expression vectors
- High cell density culturing
- Proprietary medium formulation
- Over 6000 proteins expression and purification experience
- Inclusion body refolding & soluble expression
- Protein biopharmaceutical technology

Items
- E.coli expression service
- HEK293 / CHO transient expression
- Yeast expression service
- Baculovirus-insect expression service
- CHO / HEK293 stable cell line development service

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Proteins Biology Drug Discovery Yeast

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Antibody Services

Price on request

Sino Biological has more than 10 years experience in antibody development and production, and is committed to providing scientific research institutions and pharmaceutical companies with one-stop services including antigen production, antibody development, ELISA kit development, antibody detection and production, hybridoma... Show more »

Sino Biological has more than 10 years experience in antibody development and production, and is committed to providing scientific research institutions and pharmaceutical companies with one-stop services including antigen production, antibody development, ELISA kit development, antibody detection and production, hybridoma antibody gene sequencing, chimeric antibody and antibody fragment expression, antibody humanization, antibody affinity maturation, anti-idiotype antibody and bispecific antibody production services.

Advantages
- Multiple antigen types:Polypeptide and protein antigens are two main methods for immunization and screening, various methods such as DNA, cell and insect virus immunization are also available
- Various animal species for immnization:Mouse and rabbit
Different antibody development technologies:Hybridoma technology and antibody library screening technology can be used to increase the probability of obtaining positive clones and high-specificity & high-affinity antibodies
- Diversified antibody screening technologies:Solid-phase and liquid-phase antibody screening techniques can both be used to reduce the amount of coating antigen and increase antibody diversity
- Multiple antibody detection platforms: ELISA, WB, IHC, FACS, SEC-HPLC, Octet, endotoxin, mass spectrometry and other antibody detection platforms can be used for comprehensive assessment of antibody quality
- Various antibody purification methods:Protein A, protein G, protein L, size exclusion chromatography, ion exchange chromatography, antigen affinity purification and other purification methods will be done to improve antibody purity
- Antibody recombinant expression technology:Using optimized plasmids and HEK293 cell line, Sino Biological enables the rapid, high-throughput and large-scale production of recombinant antibodies and antibody fragments
Hybridoma culture technology:Serum-free suspension culturing of hybridoma cells can be used, compared with ascites, the antibody will not include complex components and will avoid the serum influence on detection

Items
- Antibody Production & Development Services
- Rabbit Mab development Service
- Mouse Mab development Service
- Rabbit Pab development Service
- Hybridoma cell culture & antibody production Service
- Hybridoma antibody gene sequencing Service
- Antigen production Service
- ELISA Kit development Service

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Antibody Products

Price on request

Sino Biological has a professional mammalian cell expression platform with more than 10 years of process optimization and production experience, providing one-stop services from gene synthesis to high-quality antibody production. For the past 10 years, Sino Biological has provided thousands of antibodies production service to... Show more »

Sino Biological has a professional mammalian cell expression platform with more than 10 years of process optimization and production experience, providing one-stop services from gene synthesis to high-quality antibody production. For the past 10 years, Sino Biological has provided thousands of antibodies production service to customers worldwide and has established good cooperation relationships with world's leading pharmaceutical companies. Sino Biological is the preferred antibody production service provider for Top10 pharmaceutical companies worldwide.

Advantages
- Capacity and technology platform
- Rich experience
- High quality

Items
- Mini-scale Antibody Production Service (0.1mg - 1mg)
- Small and Mid-scale Antibody Production Service (1mg- 100mg)
- Large-scale Antibody Production Service (100mg - 100g)

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Recombinant Protein Expression

Price on request

Sino Biological Inc. has over 10 years' experience with 6000+ recombinant proteins production. And there is a complete system of protein expression, from gene sequence to purified protein. We have multiple protein expression systems, such as bacterial, yeast, baculovirus-insect and mammalian expression system with 30+ purification... Show more »

Sino Biological Inc. has over 10 years' experience with 6000+ recombinant proteins production. And there is a complete system of protein expression, from gene sequence to purified protein. We have multiple protein expression systems, such as bacterial, yeast, baculovirus-insect and mammalian expression system with 30+ purification systems to choose. High R & D ability with over 1000 new proteins per year, quick problem-solving ability, and close to 100 bioreactors with a variable volume between 2-1000 L to guarantee high-throughput and large-scale production allow us to serve scientists around the world.

Advantages
- High-efficiency expression vectors
- High cell density culturing
- Proprietary medium formulation
- Over 6000 proteins expression and purification experience
- Inclusion body refolding & soluble expression
- Protein biopharmaceutical technology

Items
- E.coli expression service
- HEK293 / CHO transient expression
- Yeast expression service
- Baculovirus-insect expression service
- CHO / HEK293 stable cell line development service

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Monoclonal Antibody (mAb) Production

Price on request

Advantages
- International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience in 6000+ protein production, a high success rate of antigen expression
- Professional peptide design software and high-efficiency conjugation methods ensure that the... Show more »

Advantages
- International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience in 6000+ protein production, a high success rate of antigen expression
- Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of the designed peptide is greater than 95%
- Customized immunization, screening, and detection proposed according to customer's final application requirements can be provided
- ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
- Quoting by steps, charging by nodes, reducing customer's risk and saving costs
- Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG-mediated cell fusion

Items
- Rabbit mAb Development Service
- Mouse mAb Development Service

﹥Rabbit mAb Development Service

Delivery
- Antibody H and L chains sequences for 1 positive clone
- 0.1-0.5mg purified mAb
- Vectors containing the H and L chain, respectively
- CoA
- Remaining antigen

Turnaround
- Antigen validation: 1-2 days
- Immunization and serum titer test: 8-10 weeks
- Library construction & screening: 4-6 weeks
- Antibody production & purification: 2-3 weeks
- QC analysis: 3days

Guarantee
ELISA positive for immunogen

﹥Mouse mAb Development Service

Delivery
- 2 positive clones
- 2 tubes of frozen hybridoma cells per clone
- 0.1-0.5mg purified mAb per clone
- CoA
- Remaining antigen

Turnaround
- Antigen validation: 1-2 days
- Immunization and serum titer test: 8-10 weeks
- Library construction & screening: 4-6 weeks
- Antibody production & purification: 2-3 weeks
- QC analysis: 3days

Guarantee
ELISA positive for immunogen

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Monoclonal Antibody (mAb) Development

Price on request

Rabbit mAb Development Service

Rabbit mAb usually has higher affinity and specificity than mouse mAb, it can recognize more novel epitopes and mouse antigens, and plays a key role in the development of therapeutic drug candidates and diagnostic kits. Sino Biological develops rabbit mAb by constructing an antibody library to... Show more »

Rabbit mAb Development Service

Rabbit mAb usually has higher affinity and specificity than mouse mAb, it can recognize more novel epitopes and mouse antigens, and plays a key role in the development of therapeutic drug candidates and diagnostic kits. Sino Biological develops rabbit mAb by constructing an antibody library to directly obtain antibody genes and their sequences, which is more suitable for the long-term preservation of excellent antibodies and their recombinant expression.

Advantages
- International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience in 6000+ protein production, a high success rate of antigen expression
- Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of the designed peptide is greater than 95%
- Leading antibody library construction and screening technology, reduce the risk of antibody fragment deletions or mismatches and increase the probability of screening for target antibodies
- ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
- High affinity, 10-100 times more than mouse mAb, in average
- One-stop service: From antibody development, vector construction to antibody expression & purification, saving customers time
- Quoting by steps, charging by nodes, reducing customer's risk and saving costs

Delivery
- Antibody H and L chains sequences for 1 positive clone
- 0.1-0.5mg purified mAb
- Vectors containing the H and L chain, respectively
- CoA
- Remaining antigen

Turnaround
- Antigen validation: 1-2 days
- Immunization and serum titer test: 8-10 weeks
- Library construction & screening: 4-6 weeks
- Antibody production & purification: 2-3 weeks
- QC analysis: 3days

Guarantee
ELISA positive for immunogen

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Polyclonal Antibody Production

Price on request

Rabbit Polyclonal Antibody Development Service

Rabbit pAb has been an important tool for scientific researchers because of its advantages such as short developing timeline, multiple recognition epitopes, precipitation and agglutination reactions. Sino Biological has both excellent antigen design and antibody development team,... Show more »

Rabbit Polyclonal Antibody Development Service

Rabbit pAb has been an important tool for scientific researchers because of its advantages such as short developing timeline, multiple recognition epitopes, precipitation and agglutination reactions. Sino Biological has both excellent antigen design and antibody development team, and rich experience in rabbit PAb development. We aim to develop high-quality rabbit pAb that meets customers' application requirements.

Advantages
- International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience in 6000+ protein production, a high success rate of antigen expression
- Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of the designed peptide is greater than 95%
- Serum titer ≥1:64,000
- Quoting by steps, charging by nodes, reducing customer's risk and saving costs

Delivery
- Purified pAbs from all 30-40ml serum/rabbit
- CoA
- Remaining antigen

Turnaround
- Antigen validation: 1-2 days
- Immunization and serum titer test: 8-10 weeks
- Purification: 3 days
- QC analysis: 3days

Guarantee
ELISA titer: ≥1:64,000

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Bacterial Protein Expression

Price on request

E.coli Protein Expression Service

E.coli protein expression system is now the most wide-used and economical expression system. E.coli cell possesses features of clear genetic background, high expression of target genes, short cycle, strong anti-pollution ability and easy to be scaled up. E.coli protein expression system is... Show more »

E.coli Protein Expression Service

E.coli protein expression system is now the most wide-used and economical expression system. E.coli cell possesses features of clear genetic background, high expression of target genes, short cycle, strong anti-pollution ability and easy to be scaled up. E.coli protein expression system is suitable for protein expression of multiple species, acting as a principal tool for recombinant protein expression and biotechnological industrial development, especially for small protein production. With the abundant experience of soluble protein expression and purification and protein refolding, and multiple E.coli cell lines and expression vectors, Sino Biological can provide you high-quality expression service.

Advantages
- Over 10 years' experience of protein expression
- Multiple protein purification and refolding technologies
- High-throughput and large-scale protein expression and production
- One-stop service from gene to purified protein
- Multiple expression vectors and E.coli cells to meet various expression needs

Delivery
- Sequencing report
- Feasibility report
- 1-3 mg sample (if the expression is feasible and sample remains from QC testing)
- Purified protein
- SDS-PAGE report
- CoA

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 1-2 weeks
- Expression and purification: 1-2 weeks
- Large-scale expression and purification (optional): 2-3 weeks

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Biopharmaceutical Development

Price on request

Sino Biological has a comprehensive antibody drug research and development platform, with experienced scientists and advanced equipment to provide monoclonal antibody preparation, high-throughput antibody production, antibody engineering, and biopharmaceutical analysis services involving physicochemical analysis and biological... Show more »

Sino Biological has a comprehensive antibody drug research and development platform, with experienced scientists and advanced equipment to provide monoclonal antibody preparation, high-throughput antibody production, antibody engineering, and biopharmaceutical analysis services involving physicochemical analysis and biological activities.

Advantages
- ﹥6,000 protein expression experience
- ﹥20,000 antibody screening experience
- Successfully screened dozens of candidate antibodies with good biological activity and druggability against several important drug targets
- Established cell activity evaluation models for many antibodies against key drug targets (PD-1, PD-L1, and EGFR, etc)
- A full set of preclinical research experience for multiple key drug targets

Items
- Therapeutic Antibody Development Service
- Antibody Engineering Service
- Biopharmaceutical Analysis Service
- Bioprocess Engineering Service

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Transient Protein Expression

Price on request

Sino Biological mammalian transient expression system, mainly using CHO cells and HEK293 cells as expression hosts, can provide flexible services which could meet different customer needs, including tag removal, endotoxin removal, SEC-HPLC assay, glycosylation analysis and so on.

Advantages
- High-efficiency expression... Show more »

Sino Biological mammalian transient expression system, mainly using CHO cells and HEK293 cells as expression hosts, can provide flexible services which could meet different customer needs, including tag removal, endotoxin removal, SEC-HPLC assay, glycosylation analysis and so on.

Advantages
- High-efficiency expression vectors
- Over 10 years' experience in protein expression
- Optimized mammalian expression systems
- One-stop service from gene sequence to purified protein
- High-throughput and large-scale production
- High yield expression with post-translational modification and correct folding

Delivery
- Purified protein
-SDS-PAGE report
- CoA
- Sequencing report
- Feasibility report
- 0.1-0.5 mg sample (if the expression is feasible and sample remains from QC testing)

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 1-2 weeks
- Expression and purification: 2-3 weeks
- Large-scale expression and purification (optional): 2-3 weeks

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Protein Expression in Yeast

Price on request

Yeast protein expression system, combining with the advantages of both prokaryotic system and eukaryotic system, is an economical and high-efficiency eukaryotic protein expression system. It possesses features of low-cost media, low demands for culturing conditions and suitability to scale up. Compared with the prokaryotic system,... Show more »

Yeast protein expression system, combining with the advantages of both prokaryotic system and eukaryotic system, is an economical and high-efficiency eukaryotic protein expression system. It possesses features of low-cost media, low demands for culturing conditions and suitability to scale up. Compared with the prokaryotic system, yeast expression system is capable of partial post-translational modification, such as glycosylation, acylation, lipidation, and phosphorylation. It is useful to prepare high valued protein with close to the natural structure.

Advantages
- One-stop service from gene to purified protein
- Over 10 years' experience of protein expression
- Efficient expression vectors with high yield
- High-throughput and large-scale protein production

Delivery
- Purified protein
-SDS-PAGE report
- CoA
- Sequencing report
- Feasibility report
- 0.1-0.5 mg sample (if the expression is feasible and sample remains from QC testing)

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 1 week
- Expression and purification: 1-2 weeks
- Large-scale expression and purification (optional): 2-3 weeks

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Baculovirus/Insect Cell Protein Expression

Price on request

Baculovirus-Insect Cell Protein Expression Service

Sino Biological possesses abundant experience of baculovirus-Insect cell protein expression, especially the expression of large proteins, toxic proteins, kinases, intracellular proteins and membrane proteins.

Advantages
- Multiple insect cells: Sf9, Sf21, Hi-5, etc.
-... Show more »

Baculovirus-Insect Cell Protein Expression Service

Sino Biological possesses abundant experience of baculovirus-Insect cell protein expression, especially the expression of large proteins, toxic proteins, kinases, intracellular proteins and membrane proteins.

Advantages
- Multiple insect cells: Sf9, Sf21, Hi-5, etc.
- Optimized expression vectors and higher-titer virus packaging
- High-efficiency expression of intracellular and extracellular proteins
- High-throughput and large-scale protein production

Delivery
- Purified protein
-SDS-PAGE report
- CoA
- Sequencing report
- Feasibility report
- 0.1-0.5 mg sample (if the expression is feasible and sample remains from QC testing)

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 2-3 weeks
- Expression and purification: 2-3 weeks
- Large-scale expression and purification (optional): 2-3 weeks

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Insects Proteins milligram Biology Drug Discovery

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Cell Line Generation

Price on request

The stable cell line is useful in various areas, such as recombinant protein and antibody production, testing and analysis, gene editing, functional study and so on. Engaged in protein expression study for years and possessing abundant stable cell line development experience, Sino Biological can effectively increase stable cell... Show more »

The stable cell line is useful in various areas, such as recombinant protein and antibody production, testing and analysis, gene editing, functional study and so on. Engaged in protein expression study for years and possessing abundant stable cell line development experience, Sino Biological can effectively increase stable cell line productivity and improve experimental results. Integrating all the experimental steps, we provide one-stop service from gene sequence to delivered stable cell line.

Items
- CHO stable cell line development
- HEK293 stable cell line development

﹥CHO stable cell line development
Delivery
- Purified protein
- SDS-PAGE report
- CoA
- Sequencing report
- Evaluation report
- Expression report
- 2-3 stable clones (optional)

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 2-3 weeks
- Transient expression evaluation: 2 weeks
- Stable cell line development: 12-20 weeks
- Large-scale expression and purification (optional): 2-3 weeks

﹥HEK293 stable cell line development
Delivery
- Purified protein
- SDS-PAGE report
- CoA
- Sequencing report
- Identification report
- 2 tube cells (1×10^6 cells/tube)

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 2-3 weeks
- Stable cell line development: 10-12 weeks
- Large-scale expression and purification (optional): 2-3 weeks

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Proteins Biology Drug Discovery

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Therapeutic Monoclonal Antibody (mAb) Development

Price on request

For the development of antibody drugs, the selection of targets in the early stage and the acquisition of antibodies are of utmost importance. Sino Biological has not only a comprehensive technology platform for antigen design and preparation, antibody preparation, screening, and identification, but also an optimized mouse... Show more »

For the development of antibody drugs, the selection of targets in the early stage and the acquisition of antibodies are of utmost importance. Sino Biological has not only a comprehensive technology platform for antigen design and preparation, antibody preparation, screening, and identification, but also an optimized mouse hybridoma technology platform, leading rabbit monoclonal antibody technology platform, high-throughput antibody screening, and sequencing, and high-throughput antibody production technology platform. Sino Biological is your good partner for drug target screening, who has successfully delivered tens of thousands of projects and has ample practical experience.

Advantages
- Monoclonal antibody production technology
- Comprehensive immunization methods
- Multi-species animals immunization
- Antigen preparation
- High-throughput antibody production
- Multiple high-throughput screening methods
- Multiple detection techniques

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Bispecific Antibody Production

Price on request

Bispecific antibody production service

A bispecific antibody is an artificial antibody containing two kinds of specific antigen binding sites and can establish a bridge between target cells and functional molecules (cells) to stimulate a directed immune response. It is a genetically engineered antibody, which has become a hot... Show more »

Bispecific antibody production service

A bispecific antibody is an artificial antibody containing two kinds of specific antigen binding sites and can establish a bridge between target cells and functional molecules (cells) to stimulate a directed immune response. It is a genetically engineered antibody, which has become a hot spot in the field of antibody engineering and has a broad application prospect in tumor immunotherapy. Based on the optimized mature mammalian cell expression platform, Sino Biological can provide you with fast and efficient bispecific antibody expression service.

Advantages
- Optimized HEK293 or CHO mammalian cell expression platforms: better glycosylation and post-translational modifications
- Multiple purification methods:
Protein A/G/L affinity purification
Anion and cation exchange chromatography
Size Exclusion Chromatography Column (SEC)
- Powerful QC analysis: SEC-HPLC, MS, activity assay, endotoxin test, glycosylation analysis, etc

Deliverables:
- Purified antibody
- SDS-PAGE data
- CoA

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 1-2 weeks
- Expression & purification: 1-2 weeks
- QC analysis: 3 days

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HPLC-SEC endotoxin Glycosylation Antibodies Drug Discovery

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Anti-Idiotype Antibody Production

Price on request

Sino Biological has an optimized hybridoma technology for production of mouse monoclonal antibody and proprietary rabbit monoclonal antibody technology. Sino Biological can provide paired detection antibody services that screen both free antibodies and total antibody drugs. and it can also offer you with exclusive anti-idiotypic... Show more »

Sino Biological has an optimized hybridoma technology for production of mouse monoclonal antibody and proprietary rabbit monoclonal antibody technology. Sino Biological can provide paired detection antibody services that screen both free antibodies and total antibody drugs. and it can also offer you with exclusive anti-idiotypic antibody preparation service.

Advantages
- Multiple species: mouse, rabbit, etc
- Optimized mouse hybridoma technology: higher fusion efficiency
- Serum-free hybridoma cell culture technology
- Proprietary rabbit monoclonal antibody technology: larger library size and higher affinity
- High-throughput antibody expression and screening technology

Deliverables:
- hybridoma cells
- purified antibodies and reports
- vectors containing the HC and LC gene of antibodies
- purified antibodies and reports

Turnaround
- 4-6 months

Antigen
- A client can provide enough qualified antibodies as immunogens
- Client can also provide antibody sequence for Sino Biological to express

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Antibodies Drug Discovery

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Antibody Fragment Production

Price on request

Sino Biological has developed a technology platform in mammalian cells for the expression and production of antibody Fab fragments. The recombinant antibody Fab fragments produced by our technology is of superior quality than antibody Fab fragments generated from enzyme cleavage of full antibodies.
ScFv (single-chain Fragment... Show more »

Sino Biological has developed a technology platform in mammalian cells for the expression and production of antibody Fab fragments. The recombinant antibody Fab fragments produced by our technology is of superior quality than antibody Fab fragments generated from enzyme cleavage of full antibodies.
ScFv (single-chain Fragment variable) antibody expression and production is a routine work to support antibody discovery with an antibody library. ScFv protein can be readily expressed in a microbial expression system such as E. coli. It can also be expressed in mammalian cells through transient transfection. Sino Biological is capable of producing ScFv single chain antibody fragment using either production systems, depending on customer's preference.

Advantages
- Different expression systems: E.coli, HEK293 cells, CHO cells
- Multiple tags: His-tag, FLAG-tag, HA-tag, SUMO-tag, etc.
- Various species: human, mouse, rat, rabbit, canine, rhesus, etc.

Deliverables:
- Purified antibody
- SDS-PAGE data
-CoA

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 1-2 weeks
- Expression & purification: 1-2 weeks
- QC analysis: 3 days

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Antibody Engineering

Price on request

In biopharmaceutical development, monoclonal antibodies could be engineered after the early screening. Based on engineering antibody platform and technical advantages, Sino Biological can provide a one-stop service for antibody engineering and production including chimeric antibody construction, antibody fragment production. We... Show more »

In biopharmaceutical development, monoclonal antibodies could be engineered after the early screening. Based on engineering antibody platform and technical advantages, Sino Biological can provide a one-stop service for antibody engineering and production including chimeric antibody construction, antibody fragment production. We are here to give you important support about multiple engineering attempts and better study of antibody properties.

Advantages
- Rich experience in the production of thousands of engineered antibodies
- Fastest delivery time of antibody production is two weeks
- Multiple species: human, mouse, rat, rabbit, canine, rhesus, etc
- Professional project management teams: stringently controls the entire project process, communicates with you timely about the progress and discuss issues encountered during the project
- Strict confidentiality: team members have limited access to project information and your information is strictly protected during the entire project

Items
- Chimeric Antibody Production Service
- Antibody Fragment Production Service
- Bispecific Antibody Production Service
- Anti-idiotype Antibody Development Service

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Biology Drug Discovery

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Chimeric Antibody Production

Price on request

Sino Biological has rich experience in chimeric antibody production covering not only human and mouse antibodies, but also rabbit, rat, canine antibodies. Our professional scientists and engineers are at your service to support your antibody chimerization needs.

Advantages
- HEK293 or CHO cells: better glycosylation and... Show more »

Sino Biological has rich experience in chimeric antibody production covering not only human and mouse antibodies, but also rabbit, rat, canine antibodies. Our professional scientists and engineers are at your service to support your antibody chimerization needs.

Advantages
- HEK293 or CHO cells: better glycosylation and post-translational modifications
- Multiple species:
variable region species: human, mouse, rat, canine, rhesus, chicken, rabbit, camel, etc.
constant region species: human, mouse, rat, canine, rhesus, rabbit, etc.
- Multiple subtypes: IgG, IgA, IgM, IgE

Deliverables:
- Purified antibody
- SDS-PAGE data
- CoA

Turnaround
- Gene synthesis and codon optimization (optional): 1-2 weeks
- Vector construction: 1-2 weeks
- Expression & purification: 1-2 weeks
- QC analysis: 3 days

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Biology Drug Discovery

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ELISA Kits

Price on request

Sino Biological has advanced R&D technology platform and professional technical team and has accumulated rich experience in protein expression and purification, antibody development and production, as well as various immunization detection. Sino Biological can provide development and production of ELISA kits for target molecules... Show more »

Sino Biological has advanced R&D technology platform and professional technical team and has accumulated rich experience in protein expression and purification, antibody development and production, as well as various immunization detection. Sino Biological can provide development and production of ELISA kits for target molecules in blood and other biological samples that involved in scientific research, clinical and diagnostic research, and drug development processes.

Advantages
- Abundant resources: We have 5 major protein expression and production platforms including HEK293, CHO, E.coli, Yeast, and the Insect cell. As well as optimized reagent formulation, enzyme labeling technology, and production process. Sino Biological can also customize related products and carry out kit development according to customers' need in the least time.
- Professional R&D platform:Sino Biological has developed more than 100,000 ELISA antibody in the past 10 years. We have successfully developed a 400+ ELISA pair set and 150+ ELISA kits. Sino Biological also has rich experience on ELISA kit customization for dozens of biopharmaceutical companies.
- Professional technical support: Our technical support team will provide a suitable proposal to meet customers' need fastly and efficiently.
- Quality assurance: We have various natural samples, as well as testing experience which can be used for comprehensive and accurate methodological validation of Elisa kits.
- Convenient and fast: We can provide one-stop service with fewer timelines and high efficiency, which can help the customer save time.

Delivery
- ELISA Kit
- CoA

Turnaround
- 6-12 weeks

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ELISA Biology Drug Discovery

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Hybridoma Production

Price on request

Hybridoma Cell Culture And Antibody Production Service

Compared with the ascites method for production of mAb, hybridoma cells can be cultured and purified in vitro, which has the advantages of high purity, high batch-to-batch reproducibility, less impurity and no animal virus contamination. Sino Biological provides hybridoma... Show more »

Hybridoma Cell Culture And Antibody Production Service

Compared with the ascites method for production of mAb, hybridoma cells can be cultured and purified in vitro, which has the advantages of high purity, high batch-to-batch reproducibility, less impurity and no animal virus contamination. Sino Biological provides hybridoma cell culture and antibody production services which can use low-serum or serum-free media for high-density culture in vitro and reduce bovine IgG contamination. And it has been a preferred option for domestic and foreign customers to produce antibodies using hybridoma cells.

Advantages
- > 200 hybridoma cultures and antibody production experience, success rate >99%。
- The scale of production ranges from milligrams to grams, meet the demands of different customers experiments
- Rich experience in the production of various antibody subtypes including IgG1, IgG2a, IgG2b, IgM, etc.
- The high-quality culture medium produced in-house can meet the need of high-density culture, increase yield and reduce cost
- Optimize culture and purification process to ensure the rapid and efficient purification of antibodies

Delivery
- Purified antibody
- SDS-PAGE data
- CoA

Turnaround
- Cell recovery: 1-2 days
- Adaptation: 4-6 weeks
- Production & purification: 6-10 weeks
- QC analysis: 3days

Customers need to provide
- at least 2 tubes of frozen hybridoma cells or two T25 of living cells, number of cells >1 x 10^6
- the information of antibody subtypes

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SDS-PAGE Purifications Antibodies gram milligram Biology Drug Discovery Show 7 more tags Show less

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Hybridoma Sequencing

Price on request

Hybridoma Antibody Gene Sequencing Service

During preservation, hybridoma cells may be lost antibody genes, or difficult to be recovered, or even dead, which will affect antibody production. In order to preserve and stably produce excellent antibodies for a long time, antibody genes in hybridoma cells can be extracted and... Show more »

Hybridoma Antibody Gene Sequencing Service

During preservation, hybridoma cells may be lost antibody genes, or difficult to be recovered, or even dead, which will affect antibody production. In order to preserve and stably produce excellent antibodies for a long time, antibody genes in hybridoma cells can be extracted and sequenced. Nucleotide sequences are obtained and stored as DNA for recombinant expression and production at any time.

The hybridoma antibody gene sequencing service provided by Sino Biological not only can quickly obtain the mAb gene but also can use its advanced codon optimization and gene synthesis strategies to engineer the expression and purification of the antibody.

Advantages
- Cloning of heavy- and light- chain variable region gene of mAb or antibody full-length gene
- Rich experience on primer design and antibody gene analysis
- Multiple species (human, mouse, rat, rabbit, etc.) and subtypes (IgG1, IgG2a, IgG2b, IgM, etc.) can be used for antibody gene sequencing
- One-stop service from antigen production - antibody development - hybridoma antibody gene sequencing - chimeric antibody production to humanized antibody development etc.

Delivery
- Antibody heavy and light chain sequences
- Vectors containing heavy and light chain, respectively

Turnaround
3-4 weeks

Customers need to provide
- Number of cells >1 x 10^6 or cells treated with Trizol by following steps:
a. The cells and supernatant are blown, the number of cells >1 x 10^6, cells are alive and in good condition.
b. Cells are centrifuged, and then discard the supernatant, 1-2 ml Trizol (1 x 10^6 add 1 ml, 5 x 10^6-10^7 add 2ml, 1x 10^8 add 3ml) is added according to the number of cells. Store at -80℃ and prepare for RNA extraction.
- The information of antibody subtypes
- Species and sources of hybridoma cells

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Antigen Expression and Purification

Price on request

Antigen Production Service

Our antibody development services can accept antigens provided by our customers and can also help customers produce antigens. Obtaining high-quality antigens is the key factor to successfully develop antibodies. Sino Biological can provide peptide antigens and protein antigens. Protein antigens have... Show more »

Antigen Production Service

Our antibody development services can accept antigens provided by our customers and can also help customers produce antigens. Obtaining high-quality antigens is the key factor to successfully develop antibodies. Sino Biological can provide peptide antigens and protein antigens. Protein antigens have integrity confirmation. When the protein in the sample is in its natural conformation, it is preferred to immunize with a protein antigen, such as the development of neutralizing antibodies.

If it is difficult to express the proteins or other specific detection requirements is needed, peptide antigens are economical and practical choices, such as the species-specific detection of the same protein. In addition, Sino Biological can also provide diversified immunization methods such as DNA, cells, and insect viruses to solve the protein expression problems and immunogens are closer to natural proteins.

Advantages
- International leading recombinant protein production and purification technology, with five antigen expression platforms: HEK293, CHO, E.coli, Yeast, and Insect cell, and multiple protein purification platforms such as nickel column, conjugation receptor, conjugation antibody, protein A/G, size exclusion chromatography, hydrophobic interaction chromatography and ion exchange chromatography etc.
- With the intellectual property of the medium, highly efficient expression vector and high-density cell culture systems to fully improve expression success rates and save customers cost
- The leading technical personnel and laboratory equipment, rich experience in 6000+ protein expression and purification, supporting high-throughput, large-scale and rapid production
- Professional polypeptide design software, experienced designers, and efficient conjugation solutions to ensure the immunization successful rate of the designed polypeptide is greater than 95%

Delivery
- Polypeptide sequences
- 2mg unconjugated polypeptide

Turnaround
Polypeptide antigen production: 4-6 weeks

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Insects milligram Biology Drug Discovery Yeast

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Glycosylation Analysis

Price on request

Biopharmaceutical Analysis Service

Sino Biological has highly efficient and standardized enterprise processes and is a leading biotech company focusing on early drug discovery and development services. Sino Biological has passed the ISO9001:2008 quality management system certification and can provide supreme analysis services... Show more »

Biopharmaceutical Analysis Service

Sino Biological has highly efficient and standardized enterprise processes and is a leading biotech company focusing on early drug discovery and development services. Sino Biological has passed the ISO9001:2008 quality management system certification and can provide supreme analysis services covering biological, physical, biochemical and other properties. With great expertise in biopharmaceutical analysis, we now can provide you rapidly analysis services including protein purity, molecular weight, peptide mapping, charge heterogeneity, glycosylation, thermostability, ADCC, CDC, affinity, residual host cell protein and processes related impurities, etc.
Sino Biological is equipped with LC-MS, GC-HPLC, Fluorescence Spectroscopy, Thermal Stability Analyzer, Dynamic Light Scattering, Capillary Electrophoresis, Octet Biomolecular Interaction Analysis and other advanced instruments. With rigorous scientific attitude and strict and standardized management system, Sino Biological can provide strong support for your scientific research and biopharmaceutical research work.

Advanced Equipment and Platforms for Biological Analysis
- LC-MS
- High-performance liquid chromatography (HPLC)
- Capillary isoelectric focusing electrophoresis (CIEF)
- Capillary Electrophoresis (CE)
- Octet biomolecular interaction analysis
- thermal stability analysis

Analysis
- Quantitation
- Purity test
- Size heterogeneity analysis; Molecular weight analysis; Isoelectric point test
- Peptide mapping; N-terminal Sequencing; C-terminal Sequencing; Disulfide Bond Analysis; Sequence coverage; Post-translational modification analysis; Structure analysis; Charge Heterogeneity Analysis; Iso-Asp analysis
- Residual DNA Analysis; Host Cell Protein Analysis; Residual Protein A Analysis
- Endotoxin Test; Microbial Limits Testing
- Thermostability Measurement
- Activity Assay

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ELISA qPCR HPLC-SEC SDS-PAGE LAL assay enzyme assay LC-MS Circular Dichroism assay optimization Membrane Filtration Fluorescence UV/Vis Spectrophotometry BCA CE-SDS RP-HPLC CEX-HPLC Octet CDC cellular activity assay Drug Discovery Large molecules/Biologics Show 21 more tags Show less

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Bioprocess Development and Scale-Up

Price on request

Bioprocess Engineering Service

Sino Biological has a professional and well-experienced team and technology platform that can provide a series of preclinical services covering antibody stable cell line development, detection method development and validation and product stability studies. We will help you overcome bottlenecks in... Show more »

Bioprocess Engineering Service

Sino Biological has a professional and well-experienced team and technology platform that can provide a series of preclinical services covering antibody stable cell line development, detection method development and validation and product stability studies. We will help you overcome bottlenecks in biopharmaceutical development and accelerate your R&D process.

Advantage
- A wealth of experience in expression, purification and quality control analysis
- Comprehensive program design and R&D capabilities
- A full set of preclinical research experience for multiple key drug targets

Items
- Antibody transient expression
- Stability studies
- Antibody stable cell line development

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Drug Discovery Large molecules/Biologics

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