1393886904030 ddb9918790f56fcfcead4428885d0aae

Sigma Life Science

St. Louis, Missouri, US

In late 2015, Sigma-Aldrich joined forces with life science business of Merck KGaA, Darmstadt Germany. Our combined organization, which operates as MilliporeSigma in the United States and Canada, is a global leader in the $125 billion life science industry.

Our shared purpose is to solve the toughest problems in life science by collaborating with the global scientific community. Together, we offer a broad portfolio of more than 300,000 products, including many of the most highly respected brands in the industry. Our offerings span every step of the biotech/biopharma production chain, creating a complete end-to-end workflow with enhanced customer service, a simplified interface and a leading e-commerce and distribution platform.

MilliporeSigma operates 65 manufacturing sites worldwide and employs more than 19,000 people in 66 countries. This geographic reach, combined with our innovative portfolio,... Show more »

In late 2015, Sigma-Aldrich joined forces with life science business of Merck KGaA, Darmstadt Germany. Our combined organization, which operates as MilliporeSigma in the United States and Canada, is a global leader in the $125 billion life science industry.

Our shared purpose is to solve the toughest problems in life science by collaborating with the global scientific community. Together, we offer a broad portfolio of more than 300,000 products, including many of the most highly respected brands in the industry. Our offerings span every step of the biotech/biopharma production chain, creating a complete end-to-end workflow with enhanced customer service, a simplified interface and a leading e-commerce and distribution platform.

MilliporeSigma operates 65 manufacturing sites worldwide and employs more than 19,000 people in 66 countries. This geographic reach, combined with our innovative portfolio, industry-leading capabilities, and a culture that fosters curiosity and collaboration, uniquely positions us to anticipate and fulfill our customers’ needs – and solve their toughest life science problems.

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Sigma Life Science has not listed any services.

Oligonucleotide Synthesis
Price on request

RNA Oligos

RNA Oligos are single-stranded, non-siRNA oligos for various molecular applications.
Specifications
• Synthesis scales = 0.05, 0.2 & 1.0 µmol (inquire for mg to g quantities)
• Purifications = Desalt, Cartridge, HPLC & PAGE
• Sequence Lengths = 7 – 45 bases
• Backbone = Phosphodiester /... Show more »

RNA Oligos

RNA Oligos are single-stranded, non-siRNA oligos for various molecular applications.
Specifications
• Synthesis scales = 0.05, 0.2 & 1.0 µmol (inquire for mg to g quantities)
• Purifications = Desalt, Cartridge, HPLC & PAGE
• Sequence Lengths = 7 – 45 bases
• Backbone = Phosphodiester / Phosphorothioate
• Modifications = Amines, Biotins, 2'-OMe, etc.
• Quality control: 100% mass spectrometry
• Formats = Dry in tubes
Keywords: RNA oligos, RNA oligonucleotides, single-stranded RNA oligos, single-stranded RNA oligonucleotides, oligo synthesis, oligonucleotide synthesis

Long Oligos

Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long-oligo synthesis. The techniques we have developed to purify long oligos are unmatched by other suppliers.
Specifications
• Amounts = 5 OD (Cartridge) & 0.5 OD (PAGE)
• Purifications = Cartridge & PAGE
• Sequence lengths = 121 – 180 bases
• Backbones: Phosphodiester / Phosphorothioate
• Modifications = Phosphate, Biotin, Amine C6, Amine C12, Inosine, dUracil & Spacer C3
• Quality control: 100% mass spectrometry
• Formats = Dry in tubes
For sequence lengths of 10 – 120 bases, see Custom DNA Oligos.
Keywords: gene synthesis oligos, gene synthesis oligonucleotides, CRISPR oligos, CRISPR oligonucleotides, synthetic biology oligos, synthetic biology oligonucleotides

iScale Oligos™ DNA & siRNA

iScale Oligos™ DNA & siRNA
iScale Oligos are milligram quantities of custom DNA and siRNA oligonucleotides for in vivo, high-throughput, and commercial projects (life science research tools, molecular diagnostics, and laboratory developed tests).
Specifications
• Amounts = 10, 25, 50, 100, 250 & 500 mg(1 – 5 g)
• Purifications = Desalt (≤5 g), HPLC (≤1 g) & in vivo grade
• Sequence lengths = 10 – 50 bases (inquire for longer sequences)
• Backbones: Phosphodiester / Phosphorothioate
• Modifications = Biotin, Biotin TEG, Biotin dT, phosphate, Amino-Modifier (C3, C6, C6 dT, C7, C12), TET™, HEX™, 5-Me-dC, Spacer (12, 18, C3), dSpacer, Thiol-Modifier-C3 S-S, & 2'-OMe

Easy Oligo

Order Easy Oligo when the focus is speed, convenience and simplicity. Easy Oligos are shipped within 24 hours from order receipt, at a fixed concentration, and are suitable for a wide range of applications, including PCR and sequencing.
Specifications
• Amount = 3 OD (~15 nmol)
• Purification: Desalt
• Sequence lengths = 15 – 30 bases
• Backbone: Phosphodiester
• Modifications = none
• Quality control: 100% mass spectrometry
• Format = 100 µM water in tubes
• Order volume = ≤48 oligos per 24-hours
Not available in all countries. For dry primers delivered quickly, see Pure & Simple Primers. For more complex oligonucleotide needs, see Custom DNA Oligos.
Keywords: oligos, oligonucleotides, oligo synthesis, oligonucleotide synthesis
• Quality control: 100% mass spectrometry
• Formats = Tubes
o Pooling & aliquoting
o Dry or in water / buffer
• Services = in vivo-grade purification with salt exchange, filtration & endotoxin testing
For standard synthesis scales for classical molecular biology applications, see Custom DNA Oligos.
Keywords: oligos for high-throughput applications, oligos for commercialization, in vivo grade oligos, oligo synthesis, oligonucleotide synthesis

Custom DNA Oligos

Whether you need PCR/qPCR primers or modified oligonucleotides for other classical molecular biology applications, order Custom DNA Oligos.
Specifications
• Synthesis scales: 0.025, 0.05, 0.2, 1.0, 10 & 15 µmol
• Purifications: Desalt, Cartridge, HPLC & PAGE
• Sequence lengths: 10 – 120 bases
• Backbones: Phosphodiester / Phosphorothioate
• Modifications: over 200 available
• Quality Control: 100% mass spectrometry
• Formats: Tubes, plates
o Pooling & aliquoting
o Dry or in water / buffer
Assay design support is available. For milligram / gram quantities of material, see our iScale Oligos product. For sequences 121 – 180 bases, see our Long Oligos product.
Keywords: oligos, oligonucleotides, oligo synthesis, oligonucleotide synthesis, primers, PCR primers, qPCR primers, modified oligos, modified oligonucleotides, primer design

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Oligonucleotide Probes
Price on request

Dual-Labeled Probes

Dual-Labeled Probes, used in the 5' nuclease assay, are the most common probe type for qPCR and are often referred to as hydrolysis probes.
Specifications
• Amounts: 1, 3, 5 & 10 OD
• Purification: HPLC
• Sequence lengths: 15 – 40 bases
• Modifications: LNA available
• Dyes: Comprehensive... Show more »

Dual-Labeled Probes

Dual-Labeled Probes, used in the 5' nuclease assay, are the most common probe type for qPCR and are often referred to as hydrolysis probes.
Specifications
• Amounts: 1, 3, 5 & 10 OD
• Purification: HPLC
• Sequence lengths: 15 – 40 bases
• Modifications: LNA available
• Dyes: Comprehensive offering of reporters & quenchers
• Quality Control: 100% mass spectrometry
• Formats: Supplied dry in amber tubes
Assay design support is available. For accompanying primers, see our Custom DNA Oligos or Pure & Simple Primers products.
Keywords: oligos, oligonucleotides, oligo synthesis, oligonucleotide synthesis, qPCR Probes, TaqMan

Scorpions® Probes

Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.
Specifications
• Amounts: 1, 3 & 5 OD
• Purification: HPLC
• Sequence lengths: 30 – 60 bases
• Modifications: LNA available
• Dyes: Comprehensive offering of reporters & quenchers
• Quality Control: 100% mass spectrometry
• Formats: Supplied dry in amber tubes
Assay design support is available. For an accompanying primer, see our Custom DNA Oligos or Pure & Simple Primers products.
Keywords: oligos, oligonucleotides, oligo synthesis, oligonucleotide synthesis, qPCR Probes

Molecular Beacons

Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.
Specifications
• Amounts: 1, 3, 5 & 10 OD
• Purification: HPLC
• Sequence lengths: 15 – 40 bases
• Modifications: LNA available
• Dyes: Comprehensive offering of reporters & quenchers
• Quality Control: 100% mass spectrometry
• Formats: Supplied dry in amber tubes
Assay design support is available. For accompanying primers, see our Custom DNA Oligos or Pure & Simple Primers products.
Keywords: oligos, oligonucleotides, oligo synthesis, oligonucleotide synthesis, qPCR Probes

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siRNA Synthesis
Price on request

Fast siRNA

Expedite your siRNA order with priority in the production process. The TAT is only 3 days. Not available in all countries.
Specifications
• Amounts: 10 & 25 nmol
• Purifications: Desalt
• Sequence lengths: 19 – 23 bases
• Modifications: None
• Quality Control: 100% mass spectrometry
• Formats: 50 or... Show more »

Fast siRNA

Expedite your siRNA order with priority in the production process. The TAT is only 3 days. Not available in all countries.
Specifications
• Amounts: 10 & 25 nmol
• Purifications: Desalt
• Sequence lengths: 19 – 23 bases
• Modifications: None
• Quality Control: 100% mass spectrometry
• Formats: 50 or 100 µM in tubes
Keywords: RNAi, gene silencing, siRNA synthesis, custom siRNA

Custom siRNA

Tailor your unique Custom siRNA sequence with a wide range of modifications, purifications and quantities to meet your RNAi needs.
Specifications
• Amounts: 10, 25, 50 & 250 nmol
• Purifications: Desalt & HPLC
• Sequence lengths: 19 – 27 bases
• Backbones: Phosphodiester / Phosphorothioate
• Modifications: 2'-OMe, Amines, Cyanine dyes, 6-FAM™, Biotin, Phosphate, Thiol, Abasic & 2'-Fluoro.
• Quality Control: 100% mass spectrometry
• Formats: Tubes, plates
o Pooling & aliquoting
o Dry or in water / buffer
Keywords: RNAi, gene silencing, siRNA synthesis, custom siRNA

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Custom siRNA Library
Price on request
Request a quote for more information about this service.
CRISPR-Mediated Targeted Gene Integration
Price on request

CRISPR-Mediated Targeted Integration in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers targeted integration in iPS and ES cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with... Show more »

CRISPR-Mediated Targeted Integration in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers targeted integration in iPS and ES cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom targeted integration service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Production of an oligonucleotide or plasmid donor
  • Nucleofection of the nuclease, gRNA(s), and donor
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

CRISPR-Mediated Targeted Integration in Immortalized Cells

Cell Design Studio at MilliporeSigma offers targeted integration in immortalized cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom targeted integration service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Production of an oligonucleotide or plasmid donor
  • Nucleofection of the nuclease, gRNA(s), and donor
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

« Show less
ES cells
iPS cells
Immortalized cells
CRISPR-Mediated SNP Modification
Price on request

CRISPR-Mediated SNP Modification in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers SNP modification in iPS and ES cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either... Show more »

CRISPR-Mediated SNP Modification in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers SNP modification in iPS and ES cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Production of an oligonucleotide donor
  • Nucleofection of the nuclease, gRNA(s), and donor
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

CRISPR-Mediated SNP Modification in Immortalized Cells

Cell Design Studio at MilliporeSigma offers SNP modification in immortalized cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Production of an oligonucleotide donor
  • Nucleofection of the nuclease, gRNA(s), and donor
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

« Show less
ES cells
iPS cells
Immortalized cells
CRISPR/Cas9 Knockout Cell Lines
Price on request

CRISPR-Mediated Gene Knockout in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers knockout iPS and ES cell line production using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your... Show more »

CRISPR-Mediated Gene Knockout in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers knockout iPS and ES cell line production using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Nucleofection of the nuclease and gRNA(s)
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

CRISPR-Mediated Gene Knockout in Immortalized Cells

Cell Design Studio at MilliporeSigma offers knockout immortalized cell line production using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Nucleofection of the nuclease and gRNA(s)
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

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ES cells
iPS cells
Immortalized cells
CRISPR/Cas9 Vector Construction
Starting at $500.00 per clone

As the first company to provide genome editing tools to the global research community, Sigma-Aldrich has leveraged the expertise of its best-in-class bioinformatics and manufacturing labs to develop an extensive line of CRISPR/Cas9 reagents.

Sigma CRISPRs Plasmid:
All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression... Show more »

As the first company to provide genome editing tools to the global research community, Sigma-Aldrich has leveraged the expertise of its best-in-class bioinformatics and manufacturing labs to develop an extensive line of CRISPR/Cas9 reagents.

Sigma CRISPRs Plasmid:
All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids
• A codon optimized Cas9 protein and a gRNA are expressed from a single vector and provided as ready-to-use, transformation-grade DNA.
• Unique CRISPR sites pre-designed to minimize off-targeting are available for the coding regions of the human, mouse, and rat genomes. Custom designs for any other regions or species are always available upon request.
• GFP or RFP is fused to the C-terminus of Cas9 with a 2A peptide, enabling tracking of transfection efficiency and enrichment of genome editing activity through FAC sorting.

Sigma CRISPR Cas9 mRNA:
• Wild type Cas9 or Cas9-D10A nickase available
• The Cas9 mRNA is capped and polyA-tailed for increased stability.

Sigma CRISPR guide RNA - purified RNA
• Ready-to-use gRNAs in a purified RNA format suitable for microinjection or cell culture.
• The CRISPR RNA format is ideal for researchers creating transgenic animal models or for those concerned about genomic plasmid integration.
• The RNA format avoids the need for a specific promoter, allowing for expression in most known cell types and organisms.

*This listing is for the reagents only. We do not perform the service.

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ZFN-Mediated Targeted Integration in IPS and ES Cells
Price on request

Cell Design Studio at MilliporeSigma offers targeted integration in iPS and ES cell lines using the highly specific zinc finger nuclease genome editing system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. We can engineer either your cells,... Show more »

Cell Design Studio at MilliporeSigma offers targeted integration in iPS and ES cell lines using the highly specific zinc finger nuclease genome editing system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Production of an oligonucleotide or plasmid donor
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated Targeted Integration in Immortalized Cells
Price on request

Cell Design Studio at MilliporeSigma offers targeted integration in immortalized cell lines using the highly specific zinc finger nuclease genome editing system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. We can engineer either your... Show more »

Cell Design Studio at MilliporeSigma offers targeted integration in immortalized cell lines using the highly specific zinc finger nuclease genome editing system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Production of an oligonucleotide or plasmid donor
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated SNP Modification in IPS and ES Cells
Price on request

Cell Design Studio at MilliporeSigma offers SNP modification in iPS and ES cell lines using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

Cell Design Studio at MilliporeSigma offers SNP modification in iPS and ES cell lines using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Production of an oligonucleotide donor
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated SNP Modification in Immortalized Cells
Price on request

Cell Design Studio at MilliporeSigma offers SNP modification in immortalized cell lines using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

Cell Design Studio at MilliporeSigma offers SNP modification in immortalized cell lines using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Production of an oligonucleotide donor
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated Knockout in IPS And ES cells
Price on request

Cell Design Studio at MilliporeSigma offers knockout iPS and ES cell line production using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

Cell Design Studio at MilliporeSigma offers knockout iPS and ES cell line production using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Nucleofection of ZFNs
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated Genome Editing
Zinc Finger Nuclease-Mediated Genome Editing
Price on request

Cell Design Studio at MilliporeSigma offers knockout immortalized cell line production using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

Cell Design Studio at MilliporeSigma offers knockout immortalized cell line production using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Nucleofection of ZFNs
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

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Cas9-Expressing Cell Lines
Price on request

Cell Design Studio at MilliporeSigma offers custom production of Cas9-expressing cell lines for your CRISPR screening and engineering needs. Our service utilizes highly specific zinc finger nucleases to introduce the coding sequence for wild-type Cas9 or Cas9-D10A nicking nuclease into a safe harbor locus with a variety of custom... Show more »

Cell Design Studio at MilliporeSigma offers custom production of Cas9-expressing cell lines for your CRISPR screening and engineering needs. Our service utilizes highly specific zinc finger nucleases to introduce the coding sequence for wild-type Cas9 or Cas9-D10A nicking nuclease into a safe harbor locus with a variety of custom options such as fluorescent cassettes, loxP sites, and different promoters. We can carry out the work in either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom Cas9-expressing cell line production service includes:

  • Comprehensive evaluation the cell line
  • Production of validated ZFNs
  • Production of a plasmid donor (Cas9 or Cas9-D10A)
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
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Chemical Synthesis
Price on request

Rapid Custom Synthesis (RCS)
From small-scale to bulk quantities, let us help you get what you need.

RCS is Optimal for Synthesizing:

-Starting materials
-Catalysts and ligands
-Intermediates
-Reference standards
-Authentic metabolite markers
-Lead-like or drug-like compounds
-Analog/SAR sets
-Catalyst & ligand... Show more »

Rapid Custom Synthesis (RCS)
From small-scale to bulk quantities, let us help you get what you need.

RCS is Optimal for Synthesizing:

-Starting materials
-Catalysts and ligands
-Intermediates
-Reference standards
-Authentic metabolite markers
-Lead-like or drug-like compounds
-Analog/SAR sets
-Catalyst & ligand set screening

Advantages

-By using the RCS solution from Sigma-Aldrich, your organization can access air-sensitive chemistries with confidence.
-Our RCS team has over 30 years of experience in handling pyrophoric materials.
-Nearby facilities allow for quick overnight delivery of products to US-based customers.

Capabilities

-Gram quantities of product delivered to your lab in as little as 4 weeks
-Capacity of 12+ projects per month
-Particular expertise in synthesis of homogeneous catalysts, ligands, and reagents.

Made-to-Order

-Custom products can be synthesized using methods designed by the RCS team or methods designed by your chemists.
-RCS products are backed by analytical data to suit your needs.
-Products can be offered on an exclusive basis or stocked for quick reorder availability.

Planning for Success

-Wide range of seamless scale-up options available.
-Potential for transfer to SAFC facilities.

Product Information required
*Product Name:
Synonyms and/or Acronyms:
Molecular Formula:
CAS Number:
*Quantity (grams):
Special Requirements (if any):
SMILES String
references to publications (if known):
*Application:
Is Product known in Literature?

*= Required information

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gram
milligram
kilogram
Human Biobanking
Price on request

Cell Line Expansion and BioBanking

Cell Line Expansion and BioBanking

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Plasmids and Vectors
Price on request
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Biochemistry & Molecular Biology
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Chemistry and Materials
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Biology
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Biospecimens
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DNA Synthesis and Probe Development
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DNA Synthesis and Probe Development Services

DNA Synthesis and Probe Development Services

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Synthetic Chemistry
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Genetic Engineering
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Biospecimen Management and Storage
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Mammalian Cell Culture
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Cells and Tissues
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RNA Services
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RNA Services

RNA Services

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Predesigned siRNA
Price on request

Order an extensive collection of human, mouse and rat siRNA. Multiple sequences are available for every gene with rankings to indicate performance.
Specifications
• Amounts: 10, 25, 50 & 250 nmol
• Purifications: Desalt & HPLC
• Sequence lengths: 21 bases
• Modifications: None
• Quality Control: 100% mass... Show more »

Order an extensive collection of human, mouse and rat siRNA. Multiple sequences are available for every gene with rankings to indicate performance.
Specifications
• Amounts: 10, 25, 50 & 250 nmol
• Purifications: Desalt & HPLC
• Sequence lengths: 21 bases
• Modifications: None
• Quality Control: 100% mass spectrometry
• Formats: dry in tubes
o Pooling & aliquoting
Keywords: RNAi, gene silencing, siRNA synthesis, predesigned siRNA

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Predesigned PCR Assay Probes
Price on request

KiCqStart® Probe Assays

KiCqStart Probe Assays are predesigned probe assays for gene expression analysis via RT-qPCR. Sequences provided with delivery.
Specifications
Design characteristics
• GC Content: 20 - 80%
• Melting Temperature: 58.0 °C
• Secondary Structure: None
• Excessive 3′ Primer Clamping:... Show more »

KiCqStart® Probe Assays

KiCqStart Probe Assays are predesigned probe assays for gene expression analysis via RT-qPCR. Sequences provided with delivery.
Specifications
Design characteristics
• GC Content: 20 - 80%
• Melting Temperature: 58.0 °C
• Secondary Structure: None
• Excessive 3′ Primer Clamping: Minimal
• Amplicon size: 75 - 150 bases
• Splice variant detected: Most prevalent
Oligo Characteristics (primers)
• Molecule: DNA (all sequences verified by mass spec QC)
• Internal Modifications: None
• Contents: 1 forward and 1 reverse primer
• Length: 18 - 27 bases
• Purification: Cartridge
• Format: 3 OD minimum yield, dry; 2 tubes with 1 oligonucleotide each
Oligo Characteristics (probe)
• Molecule: DNA (all sequences verified by mass spec QC)
• Internal Modifications: None
• 5’ Reporter Dye: 6-FAM™ or HEX™
• 3’ Quencher: Onyx Quencher™ A (OQ™A)
• Contents: 1 Dual-Labeled Probe
• Length: 21 - 27 bases
• Purification: HPLC
• Format: 1 OD minimum yield, dry; 1 tube with 1 oligonucleotide
Available for most genes from human, mouse, and rat.
Keywords: gene expression analysis, predesigned probes, predesigned qPCR assay, TaqMan

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Predesigned PCR Primers
Price on request

KiCqStart® Primers

KiCqStart Primers are predesigned SYBR® Green primers for gene expression analysis via RT-qPCR. Sequences provided with delivery.
Specifications
Design characteristics
• GC Content: 20 - 80%
• Melting Temperature: 55.0 ± 5 ºC
• Secondary Structure: None
• Excessive 3′ Clamping: Minimal
• Homology:... Show more »

KiCqStart® Primers

KiCqStart Primers are predesigned SYBR® Green primers for gene expression analysis via RT-qPCR. Sequences provided with delivery.
Specifications
Design characteristics
• GC Content: 20 - 80%
• Melting Temperature: 55.0 ± 5 ºC
• Secondary Structure: None
• Excessive 3′ Clamping: Minimal
• Homology: None
Oligo Characteristics
• Molecule: DNA (all sequences verified by mass spec QC); no modifications
• Contents: 1 forward and 1 reverse primer
• Length: 18 - 24 bases
• Purification: Cartridge
• Format: 3 OD minimum yield, dry; 1 oligonucleotide per tube
Available for most genes from human, mouse, rat, zebrafish, cow, horse, Arabidopsis, chicken, dog, rabbit, pig, cat, rice, soybean, rhesus, Drosophila, C. elegans, corn, X. laevis, Guinea Pig, and Chinese Hamster.
Keywords: gene expression analysis, SYBR Green primers, predesigned primers, qPCR primers

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FRET Probes
Price on request

LightCycler® Probes

LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.
Specifications
• Amounts: 0.1, 0.25, 1.5 & 15 OD
• Purification: HPLC
• Sequence lengths: 15 – 40 bases
• Modifications: LNA available
• Dyes: Probe 1... Show more »

LightCycler® Probes

LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.
Specifications
• Amounts: 0.1, 0.25, 1.5 & 15 OD
• Purification: HPLC
• Sequence lengths: 15 – 40 bases
• Modifications: LNA available
• Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)
• Quality Control: 100% mass spectrometry
• Formats: Supplied dry in amber tubes
Assay design support is available. For accompanying primers, see our Custom DNA Oligos or Pure & Simple Primers products.
Keywords: oligos, oligonucleotides, oligo synthesis, oligonucleotide synthesis, qPCR Probes

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PCR
Price on request

PCR Services

PCR Services

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NGS Oligos
Price on request

Next-Gen Sequencing Oligos
Market-leading Next-Gen Sequencing Oligos are custom universal and index (barcode) adapter oligos for major sequencing platforms. They come with suitable purity (percent full-length sequence) to maintain sequence read integrity as well as low levels of cross-contamination to facilitate proper sequence... Show more »

Next-Gen Sequencing Oligos
Market-leading Next-Gen Sequencing Oligos are custom universal and index (barcode) adapter oligos for major sequencing platforms. They come with suitable purity (percent full-length sequence) to maintain sequence read integrity as well as low levels of cross-contamination to facilitate proper sequence assembly. Three options are available: NGSO-Bronze, Silver, and Gold
Specifications
• NGSO-Bronze
o Cross contamination: <2%
o Scale & Yield: 0.05 µmol (1 – 3 OD), 0.2 µmol (3 – 8 OD) & 1.0 µmol (9 – 13 OD)—inquire for larger scales
o Purity: 70 – 85%
o Sequence Lengths: 10 – 80 bases
o Modifications: 5'-Phosphate, Phosphorothioate & 5-Me-dC
o Format: Dry in tubes
• NGSO-Silver
o Cross contamination: <1%
o Scale & Yield: 0.2 µmol (10 nmol or 2 – 6 OD depending on sequence length)—inquire for larger scales
o Purity: 65 – 80%
o Sequence Lengths: 10 – 80 bases
o Modifications: 5'-Phosphate, Phosphorothioate & 5-Me-dC
o Format: Supplied in solution in tubes or 96-well plates
 Standard: 100 µM in water, TE (10 mM Tris, pH 7.5-8.0, 1 mM EDTA ) or Tris (10 mM Tris-HCl, pH 8.0)
 Inquire: 30-150 µM in water or 30-130 µM in TE / Tris buffer
 Mixing available: Yes
 Duplexing available: No
• NGSO-Gold
o Cross contamination: <0.25%
o Scale & Yield: 0.2 µmol (3 – 8 OD) & 1.0 µmol (9 – 13 OD) or Yield: 25, 50, 100, 250 & 500 nmol—inquire for mg quantities
o Purity: 85 – 95%
o Sequence Lengths: 10 – 80 bases
o Modifications: 5'-Phosphate, Phosphorothioate & 5-Me-dC
o Format: Tubes
 Pooling & aliquoting
 Dry or in water / buffer
Keywords: next-generation sequencing oligos, next-generation sequencing oligonucleotides, NGS oligos, NGS oligonucleotides, next-generation sequencing adapters, NGS adapters

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PCR Primer Design
Price on request

Order Pure and Simple Primers, and you will be assured a quality start to all of your research applications with our ultra-high base coupling efficiency and optimized cartridge purification process.
Specifications
• Amount = 3 OD
• Purification = Cartridge
• Sequence lengths = 12 – 35 bases
• Backbone:... Show more »

Order Pure and Simple Primers, and you will be assured a quality start to all of your research applications with our ultra-high base coupling efficiency and optimized cartridge purification process.
Specifications
• Amount = 3 OD
• Purification = Cartridge
• Sequence lengths = 12 – 35 bases
• Backbone: Phosphodiester
• Modifications = None
• Quality control: 100% mass spectrometry
• Format = Dry in tubes
• Ships within 24 – 48 hours
For oligonucleotides in solution delivered quickly, see Easy Oligo
Keywords: primers, PCR primers, qPCR primers, primer design

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