1393886904030 ddb9918790f56fcfcead4428885d0aae

Sigma Life Science

St. Louis, Missouri, US

Sigma-Aldrich is a leading Life Science and High Technology company whose biochemical, organic chemical products, kits and services are used in scientific research, including genomic and proteomic research, biotechnology, pharmaceutical development, the diagnosis of disease and as key components in pharmaceutical, diagnostics and high technology manufacturing. Sigma-Aldrich customers include more than 1.3 million scientists and technologists in life science companies, university and government institutions, hospitals and industry. The Company operates in 35 countries and has nearly 9,000 employees whose objective is to provide excellent service worldwide. Sigma-Aldrich is committed to accelerating customer success through innovation and leadership in Life Science and High Technology.

Sigma Life Science has not listed any services.

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Custom siRNA Library
Price on request
Request a quote for more information about this service.
CRISPR-Mediated Targeted Gene Integration
Price on request

CRISPR-Mediated Targeted Integration in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers targeted integration in iPS and ES cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with... Show more »

CRISPR-Mediated Targeted Integration in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers targeted integration in iPS and ES cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom targeted integration service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Production of an oligonucleotide or plasmid donor
  • Nucleofection of the nuclease, gRNA(s), and donor
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

CRISPR-Mediated Targeted Integration in Immortalized Cells

Cell Design Studio at MilliporeSigma offers targeted integration in immortalized cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom targeted integration service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Production of an oligonucleotide or plasmid donor
  • Nucleofection of the nuclease, gRNA(s), and donor
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

« Show less
ES cells
iPS cells
Immortalized cells
CRISPR-Mediated SNP Modification
Price on request

CRISPR-Mediated SNP Modification in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers SNP modification in iPS and ES cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either... Show more »

CRISPR-Mediated SNP Modification in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers SNP modification in iPS and ES cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Production of an oligonucleotide donor
  • Nucleofection of the nuclease, gRNA(s), and donor
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

CRISPR-Mediated SNP Modification in Immortalized Cells

Cell Design Studio at MilliporeSigma offers SNP modification in immortalized cell lines using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Production of an oligonucleotide donor
  • Nucleofection of the nuclease, gRNA(s), and donor
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

« Show less
ES cells
iPS cells
Immortalized cells
CRISPR/Cas9 Knock-Out Cell Lines
Price on request

CRISPR-Mediated Gene Knockout in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers knockout iPS and ES cell line production using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your... Show more »

CRISPR-Mediated Gene Knockout in iPS and ES Cells

Cell Design Studio at MilliporeSigma offers knockout iPS and ES cell line production using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Nucleofection of the nuclease and gRNA(s)
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

CRISPR-Mediated Gene Knockout in Immortalized Cells

Cell Design Studio at MilliporeSigma offers knockout immortalized cell line production using the CRISPR/Cas9 RNA-guided endonuclease system. Depending on your needs, we use either Cas9-D10A paired nickases for improved specificity, or wild-type Cas9. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal gRNA sequences
  • Production and validation of gRNA(s)
  • Nucleofection of the nuclease and gRNA(s)
  • Identification of CRISPR activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

« Show less
ES cells
iPS cells
Immortalized cells
CRISPR/Cas9 Vector Generation
Starting at $500.00 per clone

As the first company to provide genome editing tools to the global research community, Sigma-Aldrich has leveraged the expertise of its best-in-class bioinformatics and manufacturing labs to develop an extensive line of CRISPR/Cas9 reagents.

Sigma CRISPRs Plasmid:
All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression... Show more »

As the first company to provide genome editing tools to the global research community, Sigma-Aldrich has leveraged the expertise of its best-in-class bioinformatics and manufacturing labs to develop an extensive line of CRISPR/Cas9 reagents.

Sigma CRISPRs Plasmid:
All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids
• A codon optimized Cas9 protein and a gRNA are expressed from a single vector and provided as ready-to-use, transformation-grade DNA.
• Unique CRISPR sites pre-designed to minimize off-targeting are available for the coding regions of the human, mouse, and rat genomes. Custom designs for any other regions or species are always available upon request.
• GFP or RFP is fused to the C-terminus of Cas9 with a 2A peptide, enabling tracking of transfection efficiency and enrichment of genome editing activity through FAC sorting.

Sigma CRISPR Cas9 mRNA:
• Wild type Cas9 or Cas9-D10A nickase available
• The Cas9 mRNA is capped and polyA-tailed for increased stability.

Sigma CRISPR guide RNA - purified RNA
• Ready-to-use gRNAs in a purified RNA format suitable for microinjection or cell culture.
• The CRISPR RNA format is ideal for researchers creating transgenic animal models or for those concerned about genomic plasmid integration.
• The RNA format avoids the need for a specific promoter, allowing for expression in most known cell types and organisms.

*This listing is for the reagents only. We do not perform the service.

« Show less
ZFN-Mediated Targeted Integration in IPS and ES Cells
Price on request

Cell Design Studio at MilliporeSigma offers targeted integration in iPS and ES cell lines using the highly specific zinc finger nuclease genome editing system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. We can engineer either your cells,... Show more »

Cell Design Studio at MilliporeSigma offers targeted integration in iPS and ES cell lines using the highly specific zinc finger nuclease genome editing system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Production of an oligonucleotide or plasmid donor
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated Targeted Integration in Immortalized Cells
Price on request

Cell Design Studio at MilliporeSigma offers targeted integration in immortalized cell lines using the highly specific zinc finger nuclease genome editing system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. We can engineer either your... Show more »

Cell Design Studio at MilliporeSigma offers targeted integration in immortalized cell lines using the highly specific zinc finger nuclease genome editing system. Tagged endogenous genes, promoter reporters, transgene integration into a safe harbor locus, and more are all possible with our service. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Production of an oligonucleotide or plasmid donor
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated SNP Modification in IPS and ES Cells
Price on request

Cell Design Studio at MilliporeSigma offers SNP modification in iPS and ES cell lines using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

Cell Design Studio at MilliporeSigma offers SNP modification in iPS and ES cell lines using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Production of an oligonucleotide donor
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated SNP Modification in Immortalized Cells
Price on request

Cell Design Studio at MilliporeSigma offers SNP modification in immortalized cell lines using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

Cell Design Studio at MilliporeSigma offers SNP modification in immortalized cell lines using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom SNP modification service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Production of an oligonucleotide donor
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated Knockout in IPS And ES cells
Price on request

Cell Design Studio at MilliporeSigma offers knockout iPS and ES cell line production using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

Cell Design Studio at MilliporeSigma offers knockout iPS and ES cell line production using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Nucleofection of ZFNs
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
  • Pluripotency characterization and karyotyping of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
ZFN-Mediated Genome Editing
Zinc Finger Nuclease-Mediated Genome Editing
Price on request

Cell Design Studio at MilliporeSigma offers knockout immortalized cell line production using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

Cell Design Studio at MilliporeSigma offers knockout immortalized cell line production using the highly specific zinc finger nuclease genome editing system. We can engineer either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom knockout service includes:

  • Comprehensive evaluation of the gene target and cell line
  • Bioinformatic identification of ideal ZFN binding sites
  • Production and validation of ZFNs
  • Nucleofection of ZFNs
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones

When you team up with Cell Design Studio, you can count on timely updates and direct communication with the scientists making your cells, as well as a dedicated project manager to ensure your project progresses according to plan.

Contact us today to discuss how we can meet your cell line engineering needs!

« Show less
Cas9-Expressing Cell Lines
Price on request

Cell Design Studio at MilliporeSigma offers custom production of Cas9-expressing cell lines for your CRISPR screening and engineering needs. Our service utilizes highly specific zinc finger nucleases to introduce the coding sequence for wild-type Cas9 or Cas9-D10A nicking nuclease into a safe harbor locus with a variety of custom... Show more »

Cell Design Studio at MilliporeSigma offers custom production of Cas9-expressing cell lines for your CRISPR screening and engineering needs. Our service utilizes highly specific zinc finger nucleases to introduce the coding sequence for wild-type Cas9 or Cas9-D10A nicking nuclease into a safe harbor locus with a variety of custom options such as fluorescent cassettes, loxP sites, and different promoters. We can carry out the work in either your cells, in-house MilliporeSigma cells, or cells from a repository such as ATCC. The custom Cas9-expressing cell line production service includes:

  • Comprehensive evaluation the cell line
  • Production of validated ZFNs
  • Production of a plasmid donor (Cas9 or Cas9-D10A)
  • Nucleofection of ZFNs and donor
  • Identification of ZFN activity in the transfected pool
  • Single cell cloning and identification of the genotype of interest in clones
  • Expansion, sequence verification, cryopreservation, and safety testing of modified clones
« Show less
Compound Synthesis
Price on request

Rapid Custom Synthesis (RCS)
From small-scale to bulk quantities, let us help you get what you need.

RCS is Optimal for Synthesizing:

-Starting materials
-Catalysts and ligands
-Intermediates
-Reference standards
-Authentic metabolite markers
-Lead-like or drug-like compounds
-Analog/SAR sets
-Catalyst & ligand... Show more »

Rapid Custom Synthesis (RCS)
From small-scale to bulk quantities, let us help you get what you need.

RCS is Optimal for Synthesizing:

-Starting materials
-Catalysts and ligands
-Intermediates
-Reference standards
-Authentic metabolite markers
-Lead-like or drug-like compounds
-Analog/SAR sets
-Catalyst & ligand set screening

Advantages

-By using the RCS solution from Sigma-Aldrich, your organization can access air-sensitive chemistries with confidence.
-Our RCS team has over 30 years of experience in handling pyrophoric materials.
-Nearby facilities allow for quick overnight delivery of products to US-based customers.

Capabilities

-Gram quantities of product delivered to your lab in as little as 4 weeks
-Capacity of 12+ projects per month
-Particular expertise in synthesis of homogeneous catalysts, ligands, and reagents.

Made-to-Order

-Custom products can be synthesized using methods designed by the RCS team or methods designed by your chemists.
-RCS products are backed by analytical data to suit your needs.
-Products can be offered on an exclusive basis or stocked for quick reorder availability.

Planning for Success

-Wide range of seamless scale-up options available.
-Potential for transfer to SAFC facilities.

Product Information required
*Product Name:
Synonyms and/or Acronyms:
Molecular Formula:
CAS Number:
*Quantity (grams):
Special Requirements (if any):
SMILES String
references to publications (if known):
*Application:
Is Product known in Literature?

*= Required information

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Human Biobanking
Price on request

Cell Line Expansion and BioBanking

Cell Line Expansion and BioBanking

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Biology
Price on request
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Cells and Tissues
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Biospecimens
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Biospecimen Management and Storage
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Mammalian Cell Culture
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Genetic Engineering
Price on request
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Biochemistry & Molecular Biology
Price on request
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Nucleic Acid Services
Price on request
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DNA Services
Price on request
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DNA Libraries
Price on request
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Plasmids and Vectors
Price on request
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Chemistry and Materials
Price on request
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Synthetic Chemistry
Price on request
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