1314212146000

Shakti BioResearch LLC

135 Orders Completed
Woodbridge, Connecticut, US

About Shakti BioResearch LLC

Shakti BioResearch provides consultant and laboratory services to biotech and pharmaceutical industries as well as academic research laboratories. We bring with us over 10 years of experience in drug discovery and applied research to provide timely, focused, and meaningful results.

Shakti means energy. At... Show more »

Shakti BioResearch provides consultant and laboratory services to biotech and pharmaceutical industries as well as academic research laboratories. We bring with us over 10 years of experience in drug discovery and applied research to provide timely, focused, and meaningful results.

Shakti means energy. At Shakti BioResearch we strive to blend Shakti with Science and Sincerity.

We provide services under GLP and FDA guidelines.


Lab Team:

Ajay Bhargava, PhD
Founder and Chief Scientific Officer

  • Dr. Bhargava received PhD from Indian Institute of Science, Bangalore, India. After post-doctoral studies with Professor Sherman Weissman at Yale University, Dr. Bhargava joined Bayer Corporation. At Bayer, he worked on target identification, target validation and assay development projects in osteoarthritis, osteoporosis and oncology therapeutic areas. Within oncology, he worked on various drug discovery projects including kinases, cytotoxics and hypoxia pathway inhibitors. Dr. Bhargava led cross functional, preclinical drug discovery project teams towards Phase I clinical trials. Dr. Bhargava has authored several peer-reviewed international scientific publications.

Stanley Menacherry, PhD, MBA
Quality Assurance Officer

  • Dr. Menacherry has 25 years of experience in Analytical and Bioanalytical Chemistry. During his career he has worked in diverse industry segments ranging from food /beverages, paper /plastics, and the pharmaceutical industry. He obtained his PhD in Analytical Chemistry from Emory University (1991) while holding a research assistantship at the Centers for Disease Control. Recently employment experience includes Abbott Laboratories and Bayer Pharmaceuticals. His main areas of expertise are the development and validation of LC/MS/MS assays, GLP compliance, Design and conduct of Preclinical Pharmacokinetic studies, planning and conduct of preclinical studies for the ADME section of IND and NDA submissions. He is has an appointment as Professional in Residence at the University of New Haven, CT.

Shakti BioResearch, a Consultancy and Laboratory Service Provider, was established in 2007. It
is located at 1 Bradley Road, Suite 401, Woodbridge CT 06525.

Laboratory is well equipped to support pharmaceutical research and development. We offer a
full array of services in support of research, preclinical, and clinical trials.

Our laboratory is equipped with biological hood and incubators for cell culture work. To support
molecular biology and assay development work, our laboratory is equipped with PCR machines,
MicroLumatPlus Luminometer, Spectramax plate reader with SoftMax PRO software, BioTek
ELx405 plate washer, and other support equipment such as multichannel pipettors, table top
centrifuges, water baths, balances, pH meter, refrigerators and freezers, etc. All laboratory
equipments are calibrated regularly to maintain the integrity of our work.

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Our Services (40)


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RT-PCR

Reverse transcription polymerase chain reaction
Starting at $20.00 per sample

RNA isolation and RT-qPCR

RNA isolation and RT-qPCR

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Antigen Expression and Purification

Price on request

Protein Expression and Purification
• Bacterial, Insect and Mammalian Systems

Protein Expression and Purification
• Bacterial, Insect and Mammalian Systems

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Cell Migration Assays

Price on request
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Cell Proliferation Assays

Starting at $150.00 per cell line

An 8-point curve will be generated. Average of 2 values will be reported.
Price is quoted for a minimum of either 10 compounds or 10 cell lines to be tested at the same time.

$150/- per cell line per compound

An 8-point curve will be generated. Average of 2 values will be reported.
Price is quoted for a minimum of either 10 compounds or 10 cell lines to be tested at the same time.

$150/- per cell line per compound

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Apoptosis Assays

Price on request
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Cell Viability Assays

Price on request
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Cell-Based Compound Screening

Price on request
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Custom Cell-Based Assay Development

Price on request
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Cytotoxicity Assays

Starting at $1,000.00 per sample

Anti-proliferative IC50 will be determined using Promega's Cell Titer Glo reagent. An 8 point curve will be generated with duplicate values at each point. Experiment will be repeated and an average of two IC50 values will be reported. One compound + one control compound will be tested in three cell lines.

Anti-proliferative IC50 will be determined using Promega's Cell Titer Glo reagent. An 8 point curve will be generated with duplicate values at each point. Experiment will be repeated and an average of two IC50 values will be reported. One compound + one control compound will be tested in three cell lines.

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DNA Library Screening

Price on request
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ELISA

Enzyme-linked immunosorbent assay
Starting at $218.00 per plate

In collaboration with our affiliate NorthEast Bioanalytical Laboratories, we provide services under GLP and FDA guidelines for:

• ELISA based assays
• Translational medicine assays

$218 per ELISA with a minimum of 4 ELISA's assayed at a time.

Capabilities:

  • Kit-based, custom designed ELISA
  • Sandwich, competitive,... Show more »

In collaboration with our affiliate NorthEast Bioanalytical Laboratories, we provide services under GLP and FDA guidelines for:

• ELISA based assays
• Translational medicine assays

$218 per ELISA with a minimum of 4 ELISA's assayed at a time.

Capabilities:

  • Kit-based, custom designed ELISA
  • Sandwich, competitive, in-Cell ELISA (ICE)
  • Homogenization of cell/tissue lysates
  • Transfection/Transduction of mammalian/non-mammalian cells
  • Optimization of protocols for commercial kits
  • Analyze serum, plasma, cells, tissues
  • Analyze fluorescent/chemiluminescent/chromogenic/radioactive substrates

Equipment:

  • SpectraMax® M5e
  • Multi-channel pipets

Number of samples processed in a single experiment: 72

Typical turn-around time: 5 days

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Luciferase Assays

Price on request
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Molecular Cloning

Starting at $300.00 per clone

Subcloning $300

PCR based cloning $0.25/bp (Minimum 1kb gene)

Subcloning $300

PCR based cloning $0.25/bp (Minimum 1kb gene)

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Site-Directed Mutagenesis

Price on request
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T Cell Activation and Proliferation Assays

Price on request
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Western Blot

Price on request

Capabilities:

  • Homogenization of cell, tissue lysates
  • Sonication of cell, tissue lysates
  • Fractionate lysates prior to gel loading
  • Transfection/Transduction of mammalian/non-mammalian cells
  • In-house cell lines (a collection of 80 cell lines)
  • Protein concentration determination
  • Strip/reprobe membranes
  • Commonly... Show more »

Capabilities:

  • Homogenization of cell, tissue lysates
  • Sonication of cell, tissue lysates
  • Fractionate lysates prior to gel loading
  • Transfection/Transduction of mammalian/non-mammalian cells
  • In-house cell lines (a collection of 80 cell lines)
  • Protein concentration determination
  • Strip/reprobe membranes
  • Commonly used antibodies available
  • Optimize protocols for particular antigen/target

Visualization:

  • Chemiluminescent substrates on x-ray film
  • Ponceau S staining

Equipment:

  • XCell SureLock™
  • iBlot®
  • Wet/Dry transfers

Number of samples in a single run: 10

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Cell-Based Assays

Price on request
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Drug Discovery & Development

Price on request
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Cell Viability & Proliferation Assays

Price on request
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Specialized Cell-Based Assays

Price on request
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In vitro Reporter Gene Assays

Price on request
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Hit Identification

Price on request
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Antibody Services

Price on request
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Toxicology

Price on request
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Cell Invasion & Migration Assays

Price on request
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Immune Cell Assays

Price on request
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Protein Quantification

Price on request
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Nucleic Acid Services

Price on request
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DNA Extraction and Purification

Price on request
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Protein Services

Price on request
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Protein Expression Visualization

Price on request
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Pharmacology & Toxicology

Price on request
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Mutagenesis

Price on request
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Cell Death Assays

Price on request
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Biology

Price on request
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Drug Discovery

Price on request
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Functional & Cell Type Specific Assays

Price on request
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Biochemistry & Molecular Biology

Price on request
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PCR

Price on request

PCR Services

PCR Services

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DNA Services

Price on request

DNA Services

DNA Services

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Ajay Bhargava

Founder / Chief Scientific Officer

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  • Positive review for Western Blot:

    November 2017

    "Highly recommend Ajay and his team for the quality work and excellent turnaround time."

  • Positive review for Cell Proliferation Assays:

    November 2017

    "These guys are good. They know their stuff, they don't waste your time and their pricing is fair. Strongly recommend."

  • Positive review for Western Blot:

    May 2017

    "Ajay and his team has been doing excellent work and their deliverable are usually very fast too"

  • Positive review for Cell-Based Compound Screening:

    May 2017

    "These guys are great. Fast, reasonably priced, and they do the job right. Wish a lot more vendors on SE were like this one!"

  • Positive review for Apoptosis Assays:

    December 2016

    "I am interested in the work done by Shakti BioResearch. Regarding apoptosis assay I was hoping to see apoptosis in the form of DNA fragmented ladder and I was disappointed when not see this ladder on the gel photo. Regarding"

  • Positive review for Cell Proliferation Assays:

    December 2016

    "Excellent"

  • Positive review for Western Blot:

    November 2016

    "Know their stuff. No nonsense. No games."

  • Positive review for Western Blot:

    November 2016

    "Ajay is very responsive and flexible. Would like to work with him again"

  • Positive review for Custom Cell-Based Assay Development:

    February 2016

    "My work was done on time and responses to enquiries were prompt and am pleased with their service. I recommend them highly"

  • Positive review for Western Blot:

    March 2014

    "Ajay was very communicative, and he went out of his way to accommodate some extra items we needed for these Validation projects. I would definitely recommend working with Ajay."

  • Positive review for ELISA:

    January 2014

    "A very quick turn around time and nicely communicative"

  • Positive review for ELISA:

    September 2013

    "Excellent provider of ELISA kit validation - I would highly recommend."

  • Positive review for ELISA:

    February 2013

    "Very professional and informed."

  • Positive review for Yeast Strain Construction and Optimization:

    December 2012

    "I requested about a dozen yeast deletion strains and received them after 5 weeks with good communication throughout the process. In fairness Ajay's team hadn't worked with this protocol before, and I knew that going into the project. One concern was that the positive control PCR reactions used to validate the constructed strains were not 100% clear. Next time, I would request a test sample before proceeding to the full project phase."

Shakti BioResearch LLC has not received any endorsements.