Shakti BioResearch LLC
132 Orders Completed
Woodbridge, Connecticut, US
Shakti BioResearch provides consultant and laboratory services to biotech and pharmaceutical industries as well as academic research laboratories. We bring with us over 10 years of experience in drug discovery and applied research to provide timely, focused, and meaningful results.
Shakti means energy. At... Show more »
Shakti BioResearch provides consultant and laboratory services to biotech and pharmaceutical industries as well as academic research laboratories. We bring with us over 10 years of experience in drug discovery and applied research to provide timely, focused, and meaningful results.
Shakti means energy. At Shakti BioResearch we strive to blend Shakti with Science and Sincerity.
We provide services under GLP and FDA guidelines.
Lab Team:
Ajay Bhargava, PhD
Founder and Chief Scientific Officer
- Dr. Bhargava received PhD from Indian Institute of Science, Bangalore, India. After post-doctoral studies with Professor Sherman Weissman at Yale University, Dr. Bhargava joined Bayer Corporation. At Bayer, he worked on target identification, target validation and assay development projects in osteoarthritis, osteoporosis and oncology therapeutic areas. Within oncology, he worked on various drug discovery projects including kinases, cytotoxics and hypoxia pathway inhibitors. Dr. Bhargava led cross functional, preclinical drug discovery project teams towards Phase I clinical trials. Dr. Bhargava has authored several peer-reviewed international scientific publications.
Stanley Menacherry, PhD, MBA
Quality Assurance Officer
- Dr. Menacherry has 25 years of experience in Analytical and Bioanalytical Chemistry. During his career he has worked in diverse industry segments ranging from food /beverages, paper /plastics, and the pharmaceutical industry. He obtained his PhD in Analytical Chemistry from Emory University (1991) while holding a research assistantship at the Centers for Disease Control. Recently employment experience includes Abbott Laboratories and Bayer Pharmaceuticals. His main areas of expertise are the development and validation of LC/MS/MS assays, GLP compliance, Design and conduct of Preclinical Pharmacokinetic studies, planning and conduct of preclinical studies for the ADME section of IND and NDA submissions. He is has an appointment as Professional in Residence at the University of New Haven, CT.
Shakti BioResearch, a Consultancy and Laboratory Service Provider, was established in 2007. It
is located at 1 Bradley Road, Suite 401, Woodbridge CT 06525.
Laboratory is well equipped to support pharmaceutical research and development. We offer a
full array of services in support of research, preclinical, and clinical trials.
Our laboratory is equipped with biological hood and incubators for cell culture work. To support
molecular biology and assay development work, our laboratory is equipped with PCR machines,
MicroLumatPlus Luminometer, Spectramax plate reader with SoftMax PRO software, BioTek
ELx405 plate washer, and other support equipment such as multichannel pipettors, table top
centrifuges, water baths, balances, pH meter, refrigerators and freezers, etc. All laboratory
equipments are calibrated regularly to maintain the integrity of our work.
RNA isolation and RT-qPCR
RNA isolation and RT-qPCR
Protein Expression and Purification
• Bacterial, Insect and Mammalian Systems
Protein Expression and Purification
• Bacterial, Insect and Mammalian Systems
An 8-point curve will be generated. Average of 2 values will be reported.
Price is quoted for a minimum of either 10 compounds or 10 cell lines to be tested at the same time.
$150/- per cell line per compound
An 8-point curve will be generated. Average of 2 values will be reported.
Price is quoted for a minimum of either 10 compounds or 10 cell lines to be tested at the same time.
$150/- per cell line per compound
Anti-proliferative IC50 will be determined using Promega's Cell Titer Glo reagent. An 8 point curve will be generated with duplicate values at each point. Experiment will be repeated and an average of two IC50 values will be reported. One compound + one control compound will be tested in three cell lines.
Anti-proliferative IC50 will be determined using Promega's Cell Titer Glo reagent. An 8 point curve will be generated with duplicate values at each point. Experiment will be repeated and an average of two IC50 values will be reported. One compound + one control compound will be tested in three cell lines.
In collaboration with our affiliate NorthEast Bioanalytical Laboratories, we provide services under GLP and FDA guidelines for:
• ELISA based assays
• Translational medicine assays
$218 per ELISA with a minimum of 4 ELISA's assayed at a time.
Capabilities:
- Kit-based, custom designed ELISA
- Sandwich, competitive,... Show more »
In collaboration with our affiliate NorthEast Bioanalytical Laboratories, we provide services under GLP and FDA guidelines for:
• ELISA based assays
• Translational medicine assays
$218 per ELISA with a minimum of 4 ELISA's assayed at a time.
Capabilities:
- Kit-based, custom designed ELISA
- Sandwich, competitive, in-Cell ELISA (ICE)
- Homogenization of cell/tissue lysates
- Transfection/Transduction of mammalian/non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze fluorescent/chemiluminescent/chromogenic/radioactive substrates
Equipment:
- SpectraMax® M5e
- Multi-channel pipets
Number of samples processed in a single experiment: 72
Typical turn-around time: 5 days
Subcloning $300
PCR based cloning $0.25/bp (Minimum 1kb gene)
Subcloning $300
PCR based cloning $0.25/bp (Minimum 1kb gene)
Capabilities:
- Homogenization of cell, tissue lysates
- Sonication of cell, tissue lysates
- Fractionate lysates prior to gel loading
- Transfection/Transduction of mammalian/non-mammalian cells
- In-house cell lines (a collection of 80 cell lines)
- Protein concentration determination
- Strip/reprobe membranes
- Commonly... Show more »
Capabilities:
- Homogenization of cell, tissue lysates
- Sonication of cell, tissue lysates
- Fractionate lysates prior to gel loading
- Transfection/Transduction of mammalian/non-mammalian cells
- In-house cell lines (a collection of 80 cell lines)
- Protein concentration determination
- Strip/reprobe membranes
- Commonly used antibodies available
- Optimize protocols for particular antigen/target
Visualization:
- Chemiluminescent substrates on x-ray film
- Ponceau S staining
Equipment:
- XCell SureLock™
- iBlot®
- Wet/Dry transfers
Number of samples in a single run: 10
PCR Services
PCR Services
DNA Services
DNA Services
"Highly recommend Ajay and his team for the quality work and excellent turnaround time."
"These guys are good. They know their stuff, they don't waste your time and their pricing is fair. Strongly recommend."
"Ajay and his team has been doing excellent work and their deliverable are usually very fast too"
"These guys are great. Fast, reasonably priced, and they do the job right. Wish a lot more vendors on SE were like this one!"
"I am interested in the work done by Shakti BioResearch. Regarding apoptosis assay I was hoping to see apoptosis in the form of DNA fragmented ladder and I was disappointed when not see this ladder on the gel photo. Regarding"
"Excellent"
"Know their stuff. No nonsense. No games."
"Ajay is very responsive and flexible. Would like to work with him again"
"My work was done on time and responses to enquiries were prompt and am pleased with their service. I recommend them highly"
"Ajay was very communicative, and he went out of his way to accommodate some extra items we needed for these Validation projects. I would definitely recommend working with Ajay."
"A very quick turn around time and nicely communicative"
"Excellent provider of ELISA kit validation - I would highly recommend."
"Very professional and informed."
"I requested about a dozen yeast deletion strains and received them after 5 weeks with good communication throughout the process. In fairness Ajay's team hadn't worked with this protocol before, and I knew that going into the project. One concern was that the positive control PCR reactions used to validate the constructed strains were not 100% clear. Next time, I would request a test sample before proceeding to the full project phase."
Shakti BioResearch LLC has not received any endorsements.