SGI-DNA

Experts in Gene Synthesis and Cell Engineering

SGI-DNA’s has expertise in a range of services. Our capabilities stem from our ability to produce large, small, and complex DNA constructs using our patented Gibson Assembly® method and error correction technology. We are committed to reducing barriers associated with DNA synthesis by leveraging technology developed at the J. Craig Venter Institute and Synthetic Genomics, Inc. Our custom gene synthesis service delivers PCR fragments ready for clonining (DNA Tiles™), dsDNA constructs (Error Free Clones) from 125 bp up to 2 Mbp in length, or variant libraries) tailored to your specifications. In call cases we can provide your construct as linear dsDNA, or clone it into your vector of choice. To allow you to design the best clone possible we offer bioinformatics services) for construct design, library design and codon optimization. In addition we provide a variety of other services including custom cell line development), Next Generation Sequencing), and plasmid purification services.

• PCR fragments up to 3kb (DNA Tiles™)
• Standard genes up to 15 kb (Error Free Clones)
• Large or complex Error Free Clones up to 2 Mb
• 100% sequence verification of Error Free Clones
• Reliable and fast turnaround times
• Accurate QC and certificate of analysis
• Superior bioinformatics support and customer service
• All genes made in California, USA

BioXp 3200 - Instrument as a Service for Overnight Gene Synthesis

The BioXp 3200 is an instrument that enables customers to build synthetic DNA in the lab in an overnight run to reduce turnaround time and costs with increased flexibility of a final delilverable. Utilizing patented Gibson Assembly technology from Synthetic Genomics, the BioXp can execute dsDNA fragments, de novo fragments cloned into a custom vector, degenerate variant DNA libraries, vector builds, combinatorial assemblies, etc in an overnight run for a deliverable of up to 32 unique DNA constructs.

Please contact for more information.

Custom Synthesis of Gene Variant Libraries

Achieve Maximum Diversity with Gene Synthesis

Create more precise and diverse sets of native genes with digitally designed libraries. Gene variant DNA libraries enable targeted modification of proteins or DNA regulatory regions and simplify studies of structure function relationships. Synthetically designed variant libraries allow for more precise control over sequence diversity and overcome many inefficiencies found in classic protein mutagenesis approaches so your selection screens are dramatically more productive. At SGI-DNA we are ready to apply our expertise to create a variant library you design or assist you with the design and generation of a library tailored specifically for your project.

Features

• Generation of virtually any library type (modular, scanning, degenerate codon etc.)
• Error-free gene synthesis – 100% sequence accuracy guaranteed
• Provide your own design or consult with our expert bioinformatics team
• Libraries available as Error-Free Clones, pooled clones or as DNA Tiles™
• DNA Clones provided in our standard vectors or your custom vector
• Quality assurance to verify sequence diversity

Plasmid DNA prep ranging from high throughput Mini Preps to Giga Preps.

SGI-DNA offers you access to our proprietary bioinformatics expertise and genome editing technologies through our service for the design and generation of custom-engineered mammalian cell lines. A wide range of cellular genetic alterations are possible using our patented Gibson Assembly® method. Our trusted team will work closely with you in the design and engineering of your cell line of interest. The custom cell line generation service offered by SGI-DNA includes synthetic DNA assembly into plasmid vectors suitable for transfection into mammalian cell lines, transfection, and screening for the intended target cells. Cell lines developed with our custom engineering services can be utilized for gene knock-out studies, gene function analysis, cell-based assays for compound screening, as well as other applications.

Highlights and Uses

• Generate novel genotypes through mutations at multiple genomic loci
• Create complex multigenic DNA constructs
• Combine unique expressor, reporter, and knockdown genes in mammalian cells
• Minimize off-target risks through the use of proprietary bioinformatics algorithms
• Express any ORF with selection markers of your choice

Applications

• Protein production
• Cell-based screening
• Functional assays
• Reporter cell lines

SGI-DNA offers NGS services on a variety of platforms ranging from Illumina to the PacBio. Please let us know what your objectives are and we can recommend the best platform to achieve your research goals.

Our proprietary synthetic genomics tools and technologies are now available to the public as a service. Synthetic Genomics, Inc. has been developing and commercializing genomic driven advancements in a variety of industries. Our unique and powerful bioinformatics capabilities are already being utilized in collaborations with Monsanto to analyze microbe-plant communities and United Therapeutics Corporation to develop humanized pig organs to revolutionize the field of transplantation. Our advanced analysis tools and expertise will enable you to discover meaningful relationships within your sequence data.

Expert Bioinformatics support at your service to accomplish any number of analysis types. We work with you to make sure you are getting the correct analysis and your data stays secure.

• Expert bioinformatics team
• Full service provider
• Cost effective with quick deliver of data
• Flexible analysis format

Types of Analysis

• Variant (SNP & Indel) Discovery
• Structural Variation Discovery
• Exome Sequencing Analysis
• Differential Gene Expression Analysis
• Novel Isoform (Alternative Splicing) Identification
• Peak Analysis (ChIP-Seq)
• Small & miRNA Sequencing Analysis
• De Novo Genome & Transcriptome Assembly
• Gap-Closure Analysis (Genome Finishing)
• 16S rRNA Metagenomics Analysis
• Whole Genome Metagenomics Analysis

Our scalable annotation pipeline can deliver annotated data rapidly through Archetype. We use sophisticated algorithms to annotate any type of genome.

• Sophisticated algorithms
• Rapid pipeline
• Customizable data delivery
• Expert support