Sequetech

Our Single Primer Extension Service is offered for Plasmids, PCR Products, Single-Strand templates like M13 clones, and larger constructs such as Lambda and BAC clones. A single sequencing reaction is performed and the resulting data is provided to the customer, typically within 24 hours.

Here is what is included in this service:

• Analysis of sequencing reactions on ABI 3730xl DNA Analyzer platform.
  
• BigDye Terminator or BigDye Primer methodologies.
  
• Typically 800-1000 bases of useful sequence data.
  
• Manual inspection and editing of the electropherograms by an experienced analyst available.
  
• Full support for templates which prove to be difficult to sequence.
  
• Use of any of our In House Primers is included in this service.
  
• Quantifying template concentration

Publication Quality Sequencing Service

Our Publication Quality Sequencing Service is offered for Plasmids, PCR Products, and larger constructs such as Lambda and BAC clones. Sequencing reactions are performed; primers are designed and synthesized to allow us to "Primer Walk" the region of interest. Both strands of the region of interest are sequenced independently. The accuracy of Publication Quality sequence data is guaranteed by virtue of complete complementarity between the two strands. Project turnaround is dependent upon the size of the region to be sequenced and pre-existing knowledge of the expected sequence. Sequencing is typically completed at a rate of about 1.5 Kb to 2.0 Kb per week.

Here is what is included in this service:

• Analysis of sequencing reactions on ABI 3730xl DNA Analyzer platform
  
• Each base is independently confirmed on both strands through redundant sequencing analyses.
  
• Overlapping sequencing analyses performed on each strand.
  
• Assembly of overlapping sequence data
  
• Design and synthesis of all necessary sequencing primers
  
• Full support for templates which prove to be difficult to sequence
  
• An electronic text file of the finished sequence
  
• Quantifying template concentration
  
• An optional report which includes:
  
  • a printed copy of the entire text sequence
    
  • a translation of the finished sequence in all 6 reading frames
    
  • a map indicating the sequence data coverage and sequencing primer locations
    
  • a printed copy of the four-color electropherograms from each sequencing run
    
  • a list of the sequencing primers with their sequences
    
  • a map of the cut sites of all commercially available restriction enzymes
    
• All excess sequencing primers (optional)

Sequetech is pleased to offer Next Generation sequencing technology. We use state of the art semiconductor sequencing technology to produce up to 3 million 300 base pair reads with one day turnaround! We have selected the Ion Torrent PGM for our Next Generation sequencing platform because of its excellent balance of throughput and turnaround time. Because we can produce results so quickly and accurately, Sequetech is the ideal choice for sequencing small genomes, sets of genes, and amplicon sequencing.

Depending on your application, choosing Sequetech may be the ideal choice for your Next Generation sequencing needs. Not all Next Generation applications are ideally suited to the Ion Torrent PGM. Here is a short list of applications where Sequetech is able to provide superior service compared to other platforms:

• Extremely rapid turn around of prepped libraries. If you have prepped fragment or amplicon libraries, Sequetech can provide you with up to 3 million 300 base pair reads by the next business day!
  
• Per base, Sequetech's Next Generation sequencing is price competitive to other platforms such as Illumina - with one tenth the turn around time.
  
• Sequetech is experienced at preparing libraries from purified or raw genomic DNA or BAC Clones. We can make amplicon or fragment libraries.
  

There are a number of other applications for the Ion Torrent PGM, so feel free to contact us even if your intended application is not listed. We are happy to take the time to work with you and help you design the optimal strategy to complete your project.

Our Single-Strand Contig Service is offered for Plasmids, PCR Products, Single-Strand templates like M13 clones, and larger constructs such as Lambda and BAC clones. While not guaranteed, the accuracy of single-strand sequence data typically exceeds 99%. Sequencing Reactions are performed; primers are designed and synthesized to allow us to "Primer Walk" the region of interest. Project turnaround is dependent upon the size of the region to be sequenced and pre-existing knowledge of the expected sequence. Sequencing is typically completed at a rate of about 1.5 Kb to 2.0 Kb per week.

Here is what is included in this service:

• Analysis of sequencing reactions on ABI 3730xl DNA Analyzer platform
  
• BigDye Terminator or BigDye Primer methodologies
  
• Overlapping sequencing analyses performed on a single strand
  
• Assembly of overlapping sequence data
  
• Design and synthesis of all necessary sequencing primers using state-of-the-art primer design software
  
• Full support of templates which prove to be difficult to sequence
  
• Quantifying template concentration
  
• An electronic copy of the finished sequence
  
• A map showing location and coverage of sequence data and sequencing primer locations (optional)
  
• All excess sequencing primers (optional)
  
• Hard copies of the electropherograms, and finished sequence (optional)