SeqMatic is an Illumina Propel Certified service provided based in California. We provide comprehensive NGS services with unparalleled customer service and fast turnaround time. We are experts in DNA, RNA, and miRNA library generation, sequencing, and data analysis. Our scientists help tailor experiments to satisfy your needs with expert and friendly service.
SeqMatic accepts single cell suspensions or customer prepared 10X single cell RNA sequencing libraries for sequencing. Our streamlined service has been specially optimized to meet the needs of this unique library type.
Scalable Sequencing Solutions: We offer flexible options on the NextSeq, HiSeq, or NovaSeq to accommodate projects of any size
Expert Service: Our team of experts understand the unique challenges associated with single cell RNA sequencing
CellRanger Data Analysis: Customized FASTQ demultiplexing is included for all orders. Sequence alignment and aggregate analysis also available.
SeqMatic accepts sequencing ready libraries in addition to DNA and RNA for our high quality library generation services. All samples are quantified using our unique HT1 qPCR assay, ensuring maximum data output for each run. Dual Indexed PhiX control libraries are also used to prevent cross contamination of PhiX in your reads. All of our sequencing services feature fast turnaround time and online data delivery via our secure FTP server. Please request a quote for full pricing information.
16S sequencing enables unparalleled insight of microbial communities, enabling researchers to study a variety of microbiomes such as soil and intestinal flora. SeqMatic offers sequencing and analysis of 16S rRNA gene for bacterial communities. Dozens to hundreds samples can be sequenced and analyzed with rapid turnaround times.
Sequencing is performed on the Illumina MiSeq instrument, which is the best choice for 16S sequencing due to its ability to generate high quality data from PCR amplicons.
Variable Regions Available:
Services include free basic data analysis. Deliverables include raw data and a summary table showing the identified microbial species and it's abundance. Additional bioinformatics services are also available for an additional fee.
Please request an estimate with your total number of samples for full pricing details.
Profile all short, non-coding RNA from any organism without the need for reference sequences. Detect novel microRNAs even with low inputs using our custom developed highly sensitive methods. Practical applications include biomarker discovery and miRNA expression profiling.
SeqMatic provides comprehensive services for library generation, sequencing , and data analysis of miRNA.
RNA extraction services are also available for a wide variety of organisms and sample types. Libraries can be sequenced on the Illumina NGS instruments for fast, cost effective, high output sequencing.
Bioinformatic services also available for an additional fee.
SeqMatic provides high quality RNA sequencing services with a fast turnaround time. Strand specific RNA-Seq libraries are ideal for any RNA-Seq application including gene expression profiling, SNP detection, alternative splicing identification, and transcript annotation.
Sequencing is offered using Illumina HiSeq and NextSeq instruments. Data is delivered online in 2-4 weeks after sample submission.
Bioinformatic services also available for an additional fee.
Illumina NovaSeq 6000 sequencing service from an Illumina Propel Certified Provider.
The NovaSeq 6000 is the latest sequencing system produced by Illumina. This new instrument can generate more than 3TB of sequencing output in less than two days, enabling users to complete projects faster without sacrificing data quality.
SeqMatic accepts samples for library generation as well as customer prepared libraries. Our library QC utilizes qPCR to ensure maximum data output for each run. Use of our Dual Indexed PhiX control is included in all runs to monitor index hopping and prevent cross contamination of PhiX in your data.
Special promotional rates are currently offered for new users. Please request a quote for details.
Service Highlights:All services performed in the USA at our facility in the San Francisco Bay Area 1 week turnaround for all run types PhiX spike in included Single or dual index reads for no additional charge Free demultiplexing (FASTQ) and SFTP data transfer
Available Run Types:
1 x 100bp or 2x50bp
2 x 100bp
2 x 150bp
Illumina NextSeq Sequencing with Three Day Turnaround
Mid Output (130M Reads) or High Output (400M Reads)
Read length up to PE 2x150
Illumina MiSeq Sequencing with Three Day Turnaround
10X Single Cell Gene Expression Library Prep, Sequencing, and Data Analysis
TruSeq Stranded mRNA library preparation for Illumina NGS sequencing. Serivce includes library generation, QC, normalization, and pooling. Samples can be sequenced by us or shipped back to the customer to sequence on their own instrumentation.
High throughput PCR amplicon sequencing on Illumina MiSeq. Sequence amplicons up to 600bp at full length without assembly.
SeqMatic offers PCR amplicon library generation services in two formats. Customers may submit a pool of PCR amplicons for library prep using a ligation format or SeqMatic may help customers redesign primers for library prep using a PCR step out format. Please request a quote for details.
Illumina library generation with 1-2 week turnaround time.
DNA library prep using Illumina TruSeq or Nextera sample prep kits.
As little as 1ng of gDNA required.
Strand specific RNA library prep using Illumina TruSeq or SeqMatic Tailormix sample prep kits.
High yields and quality with 100ng of total RNA required.
Small RNA library prep using optimized SeqMatic Tailormix miRNA sample prep kits.
10ng of total RNA required.
Custom PCR amplicon library generation also available.
Bulk discounts available. Please request an estimate for details.
High quality DNA extraction from a variety of sources including difficult samples like FFPE and Trizol.
All samples will be validated using a variety of methods such as gel, bioanalyzer, A260/A280, picogreen etc.
RNA extraction from regular tissues or difficult sources including FFPE and Trizol.
Bioinformatics services available for our NGS customers. We can provide advanced bioinformatic services such as data QC, alignments and assemblies.
Bacteria NGS assembly and annotation
RNA and miRNA expression analysis
PCR amplicon analysis
QC Summary report
Data analysis report
Raw sequencing and alignment files
Custom experiment design for Illumina next generation sequencing projects.
"We have used SeqMatic numerous times for 16S analyses, and each time I am impressed with their professionalism, attention to detail, and willingness to answer questions. I will never go with another firm for this kind of work. I would rate them as one of the top companies with whom to work on NGS."
"This was our lab's second time using SeqMatic. The turnaround time was excellent, and we received our results sooner than expected. Correspondence with them was quick and they were always willing to answer my questions. I would recommend SeqMatic highly for anyone doing 16S sequencing."
"Very responsive and helpful people. Great turn-around time."
"Amazing customer service, and fast turnaround time. I will use their services for all future projects of this sort. I highly recommend SeqMatic!"
"We are very satisfied with the analyses and the report. The lab handled all the steps of the project very professionally and lab team was available for any question related to the task and beyond. Good job"
"Provided excellent quality small RNA sequencing data from tricky samples (small amounts, from FFPE tissue)."
"We are satisfied with the results and in fact I have suggest to friends that they should use SE and that Seqmatic can offer good results."
SeqMatic has not received any endorsements.