Sekisui XenoTech, LLC
About Sekisui XenoTech, LLC
XenoTech offers in vitro and in vivo ADME and PK services including: drug-drug interactions (inhibition and induction), metabolism, reaction phenotyping, metabolite characterization, ID and stability, bioanalysis, transporter services, biologics, radiolabeled synthesis, QWBA and protein binding studies. We also... Show more »
XenoTech offers in vitro and in vivo ADME and PK services including: drug-drug interactions (inhibition and induction), metabolism, reaction phenotyping, metabolite characterization, ID and stability, bioanalysis, transporter services, biologics, radiolabeled synthesis, QWBA and protein binding studies. We also offer a full catalog of in vitro ADME research products including hepatocytes, subcellular fractions, recombinant CYPs, kupffer cells, tritosomes, homogenate, media & reagents, etc.
Our Services (169)
In vivo Bioavailability/Bioequivalence Studies
Radiolabeling/Radiosynthesis
In vitro ADME/DMPK Studies
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Solute-Linked Carrier (SLC) Transporter Substrate Identification
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Solute-Linked Carrier (SLC) Transporter Inhibition Assays
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Plasma Protein Binding
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Caco-2 Cell Permeability Assay
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MDR1-MDCK Permeability Assay
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In vivo ADME/DMPK Studies
In vivo ADME lab offerings through Sekisui:
- Drug plasma concentration
- Absorption site investigation
- Tissue distributions
- QWBA (Quantitative Whole Body Autoradiography)
- Plasma protein binding
- Blood/plasma partition rate
- Lymphatic partition rate
- Metabolic profile (plama, urine, bile and tissue)
-... Show more »
In vivo ADME lab offerings through Sekisui:
- Drug plasma concentration
- Absorption site investigation
- Tissue distributions
- QWBA (Quantitative Whole Body Autoradiography)
- Plasma protein binding
- Blood/plasma partition rate
- Lymphatic partition rate
- Metabolic profile (plama, urine, bile and tissue)
- Metabolite Identification
- Excretion rate (urine, feces, expiration air and bile)
- Enterohepatic circulation
- Radiolabeled Synthesis
- Bioanalysis by ECL
- Radioreceptor Assay (RRA)
- Ocular Pharmacokinetics
Peptide Synthesis
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Chemical Synthesis
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In vivo PK/PD Studies
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Primary Cell Culture
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Cell Line Collections
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Cytotoxicity Assays
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Custom Cell-Based Assay Development
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In vitro Hepatotoxicity Testing
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Cytotoxicity Assays
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In vitro Toxicity Testing
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Liquid Chromatography Coupled Mass Spectrometry Methods
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Liquid Chromatography (LC)
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LC-MS/MS
Liquid chromatography tandem mass spectrometry
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LC-MS
Liquid Chromatography-Coupled Mass Spectrometry
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MS-MS
Tandem Mass Spectrometry
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Receptor Binding Assays
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Ligand Binding Assays
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Drug Transporter Assays
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Analytical Chemistry Services
Our Analytical Services group offers custom method validation, dose solution analysis, in vivo metabolite profiling studies and small-molecule bioanalytical services. The group's focus is on applying LC/MS technology to maximize both the quality and throughput of the data we generate to ensure that your project needs are met.
Our Analytical Services group offers custom method validation, dose solution analysis, in vivo metabolite profiling studies and small-molecule bioanalytical services. The group's focus is on applying LC/MS technology to maximize both the quality and throughput of the data we generate to ensure that your project needs are met.
Ocular Irritation Testing
Toxicology Services: In vitro toxicity testing - XenoTech utilizes the MatTek EpiOcular™ platform, a validated test system for the classification of chemicals for ocular irritation according to the OECD (Organization for Economic Cooperation & Development) Test Guideline 492. The EpiOcular™ platform is a 3D tissue culture model... Show more »
Toxicology Services: In vitro toxicity testing - XenoTech utilizes the MatTek EpiOcular™ platform, a validated test system for the classification of chemicals for ocular irritation according to the OECD (Organization for Economic Cooperation & Development) Test Guideline 492. The EpiOcular™ platform is a 3D tissue culture model comprised of non-keratinized epithelium prepared from normal human keratinocytes, which simulate the physiological properties of the cornea epithelium (RhCE) cells. The assay is able to assess test chemicals with a variety of physical properties including liquid, semi-solid, or waxy.
Compound IC50 Assays
Salt Screening
In vitro Hepatotoxicity Testing
Compound Management
Drug-Excipient Compatibility Studies
Neuropathic Pain Animal Models
Custom Pain Animal Models
Pharmaceutical Formulation
In vitro Metabolic Stability Assays
HT ADME
Natural Product Isolation and Purification
Equilibrium Dialysis
Compound Purification
Diabetes Animal Models
db/db Diabetic Mice
fa/fa Zucker Diabetic Rats
db/db Diabetic Mice
fa/fa Zucker Diabetic Rats
In silico Drug-Drug Interaction Modeling
Reactive Metabolite Assessment
Blood Partitioning
Protein Aggregation Analysis
Compound Sourcing
Multidrug Resistance (MDR) Testing
Protein Binding Ultrafiltration
Label-Free Mass Spectrometry
Ischemia Animal Models
Chemistry, Manufacturing and Controls (CMC)
CYP Inhibition Assay
Overview
XenoTech's study design evaluates drug candidates as direct, time-dependent and metabolism-dependent inhibitors of CYP enzymes in human liver microsomes. Our design allows us to screen for inhibition due to the drug added to the test system as well as inhibition due to metabolites formed during preincubation. The... Show more »
Overview
XenoTech's study design evaluates drug candidates as direct, time-dependent and metabolism-dependent inhibitors of CYP enzymes in human liver microsomes. Our design allows us to screen for inhibition due to the drug added to the test system as well as inhibition due to metabolites formed during preincubation. The design features low protein concentrations and short incubation times to minimize artifacts caused by protein binding, metabolic instability of the test article and excessive metabolism of the marker substrate. We offer various options for follow-up studies to support the further characterization of those drug candidates that are identified as potent direct or metabolism-dependent inhibitors (for example: Ki determinations, XTRA: XenoTech's Reversibility Assay, KI/kinact determinations).
Assay Selection
| Enzyme | Substrate |
|----------------|------------------------------------------------|
| CYP1A2 | Phenacetin O-dealkylation |
| CYP2A6 | Coumarin 7-hydroxylation |
| CYP2B6 | Efavirenz 8-hydroxylation |
| CYP2B6 | Bupropion hydroxylation |
| CYP2C8 | Amodiaquine N-dealkylation |
| CYP2C8 | Paclitaxel 6-hydroxylation |
| CYP2C9 | Diclofenac 4´-hydroxylation |
| CYP2C19 | S-Mephenytoin 4´-hydroxylation |
| CYP2D6 | Dextromethorphan O-demethylation |
| CYP2E1 | Chlorzoxazone 6-hydroxylation |
| CYP3A4/5 | Testosterone 6β-hydroxylation |
| CYP3A4/5 | Nifedipine oxidation |
| CYP3A4/5 | Midazolam 1´-hydroxylation |
| CYP3A4/5 | Atorvastatin ortho-hydroxylation |
| CYP4A11 | Lauric acid 12-hydroxylation |
Test System
XenoTech's inhibition assays are typically performed with a dedicated pool of human liver microsomes (pool of 16) to provide Km and Vmax values for each marker substrate reaction. This allows the same pool of human liver microsomes (and often the same experimental conditions, for example, protein concentrations and incubation time) to be used to study all CYP enzymes of interest.
Incubation Conditions
Protein concentration ≤0.1 mg/mL
Incubation time: 5 minutes
Pre-incubation time (in the presence and absence of NADPH): 30 minutes
Results
Direct, time-dependent and metabolism-dependent IC50 values are determined from seven concentrations of the drug candidate.
Membrane Protein Expression
Toxicology
Radioisotope Production
Product Stability Testing
Radiotracers and Radiopharmaceuticals
Parallel Artificial Membrane Permeability Assay (PAMPA)
Parallel Artificial Membrane Permeability Assay
Antibody Detection
In vivo Drug Efficacy Testing
Small Molecule Bioanalysis
Preclinical Study Design
In vivo Pharmacology Studies
Quality Assurance
ADME/DMPK Studies
Drug Metabolism and Pharmacokinetics
Anatomic Pathology
Histology and Pathology Services
PK/PD Data Analysis
Mass Spectrometry
Off-Target Drug Effects
Hit to Lead and Lead Optimization
Microsomal Stability Assay
Drug Discovery
Cell Permeability Assay
Drug Development
CYP Induction Assay
Bioanalytical Assays
XenoTech offers non-regulated small molecule bioanalysis services through its Analytical Services group. Highlights of XenoTech's bioanalytical capabilities include:
- Protein precipitiation extraction (others as required)
- Matrices include plasma, serum, blood and urine
- Development of single or multiple analyte methods to... Show more »
XenoTech offers non-regulated small molecule bioanalysis services through its Analytical Services group. Highlights of XenoTech's bioanalytical capabilities include:
- Protein precipitiation extraction (others as required)
- Matrices include plasma, serum, blood and urine
- Development of single or multiple analyte methods to support parent and metabolite(s) analysis or cassette analysis
- Method qualification (optional)
- Duplicate standard curves (bracketed samples)
- Flexible standard curve ranges up to 5000-fold (modify as needed)
- Sample analysis by LC-MS/MS
- Electronic data summary outlining analytical method and sample preparation, calibration curve and calculated sample concentrations
- Rapid turnaround times
Bioanalytical Analysis LC/MS/MS
Reaction Phenotyping
Analytical Chemistry Services
Nuclear Receptor Assays
Metabolite Generation
In vivo Protein Binding Assays
Protein Binding HPLC
High Resolution Mass Spectrometry
Solubility and Dissolution Testing
NMR
Nuclear magnetic resonance spectroscopy
Cell Products
Manufacturing Services
Chemical Stability Testing
Forced Degradation Studies
Custom Cell-Based Assay Development
Cell Culture Consulting
Assay Development Consulting
Histology and Pathology Services
Structure Determination
Metabolite Profiling
Immunophenotyping
Lysosomal Trapping Assay
Immunohistochemistry (IHC)
Human Hepatocytes
Assay Development
Analytical Method Development
In vivo Skin Irritation/Sensitization Testing
Toxicology Services: In vitro toxicity testing - XenoTech utilizes the MatTek EpiDerm™ platform, a validated test system for the classification of chemicals for dermal irritation, according to the OECD Test Guideline 439. The EpiDerm™ platform is a 3D tissue culture model comprised of reconstructed human epidermal (RhE) cells... Show more »
Toxicology Services: In vitro toxicity testing - XenoTech utilizes the MatTek EpiDerm™ platform, a validated test system for the classification of chemicals for dermal irritation, according to the OECD Test Guideline 439. The EpiDerm™ platform is a 3D tissue culture model comprised of reconstructed human epidermal (RhE) cells differentiated into keratinocytes, which stimulate the physiological properties of the human skin. The assay is able to assess test chemicals with a wide variety of physical properties including solids, liquids, semi-solids or waxy.
Cell viability following chemical exposure is measured using the MTT assay. The test article is then classified according to the UN GHS (United Nations Globally Harmonized System of Classification and Labelling Chemicals) according to viability following chemical exposure.
Cell and Tissue Culture
Chemistry and Materials
Specialty Mass Spectrometry Methods
Product Development, Testing, and Packaging
Engineering and Fabrication
Natural Products
Cardiovascular Animal Models
Separation/Purification Services
In vitro Bioavailability/Bioequivalence Studies
Protein Services
In vitro Disease Models
Business Development, Marketing & Operations
Synthetic Chemistry
Immunostaining
Compound Sourcing and Management
Protein Expression Visualization
In vivo Toxicity Testing
Bioanalysis
Biomolecular Interaction Analysis
Biospecimens
Tandem Mass Spectrometry Methods
Drug Absorption Studies
Omics
Project Management
Animal Models of Disease
Biomarkers
Receptor Assays
Biology
Biochemistry & Molecular Biology
Cell Culture Media and Reagents
Computational Chemistry
Drug Discovery & Development
Transporter & Cell Uptake Assays
Biochemical Assays
Human Primary Cells
Functional Human Tissue Assays
Protein Conjugation
Cell-Based Assays
Metabolomics
Lead Identification and Validation
Pharmacology & Toxicology
Radiochemistry
Cells and Tissues
Cell Death Assays
Blood Banking and Transfusion Medicine
Imaging & Spectroscopy
Process Consulting
Product Quality Control
Immunoassays
Chromatography
Animal Models and Studies
Physical Analysis Methods
Pain/Neuropathy Animal Models
Strength Analysis
Antibody Services
Human Biospecimens
Formulation Services
Cell Lines
Safety Pharmacology
Specialized Cell-Based Assays
Spectroscopy
Protein Characterization
Pharmacology
Pharmacology Services
Pharmacology Services
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Get info on this provider's capabilities without requesting a quote.Matt Beck
Territory Account Manager
Greg Loewen
Director, Business Development
Teresa Martin
Account Manager - Products
Michael Marx
Technical Advisor
Michael Millhollen
Global Marketing Manager
Zachary Mitts
Account Manager - Northwest
Sekisui XenoTech 2016 Product Tech Guide.pdf
XenoTech_catalog_PDF.pdf
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Positive review for Compound IC50 Assays:
August 2015
"It was great working with XenoTech to complete a project for one of our biopharma clients. They were easy to work with and very professional. The client was very satisfied with the quality of services. We would definitely recommend them to other clients in the future!"
Sekisui XenoTech, LLC has not received any endorsements.