SciGenom

SciGenom provides the following range of cloning services:

Traditional cloning of inserts by PCR and digestion, ligation.

DNA sub-cloning from entry to destination vector.

Fusion genes cloning using seamless or gibson DNA assembly techniques.

Knock-out / Knock-in Vector constructs for CRISPR-Cas9 genome editing.

Plasmid DNA preparation: Miniprep (10μg-30μg), Maxiprep (700μg-1.5mg).

Introduce desired sequence changes such as single / multiple point mutations, insertions, deletions and substitutions in the gene of your interest.

Cloning in E.coli expression vectors: 

Create customised constructs, choosing from the varied promoter types (T7, Tac) and fusion tags (His, GST, MBP); and get target genes cloned in these vectors.

SciGenom offers production of high-affinity monoclonal reagent-grade Fabs and full-length IgGs for use in basic and clinical research labs. It uses licensed proprietary phage display libraries to generate synthetic Fabs/IgGs. The diversity of peptides displayed in the library is >10^10, which guarantees high affinity Fabs against antigens from any source.

• Purified Ag protein (5mg/ml) can be supplied by customer against which Fab can be generated. Alternatively, customer can use our protein production pipeline to generate the antigen for screening.
• Customer has the option to select Fabs or full-length IgGs, based on their requirement.
• Option of getting the Ag-Fab or Ag-IgG validation assay optimizedis available with us
• Customer can choose Fc region of IgG (human/mouse/rat/goat/others (specify), depending on their application.
• Customer has the ability to opt for any special detection tags on the Fabs/IgGs.
• SciGenom Fabs/IgGs work best for FACS/IF/IP and ELISA and worth testing for WB and IHC.