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SBH Sciences

Natick, Massachusetts, US

SBH Sciences is a preclinical Contract Research Organization with extensive products and services of 345 human cancer cell lines, 300 bioassays, 31 recombinant cytokines, 8 recombinant Glycosyltransferase enzymes, 33 monoclonal antibodies, development of 30 ELISA kits and Multiplex analyses.

SBH Sciences is the world leader in cell-based assay to measure biological activity of cytokines and Growth Factors, and has provided services to over 150 biotechnology companies across the globe in our 18 years of business. Our proprietary processes have achieved commercial production of cytokines, new enzymes and monoclonal antibodies.

SBH Sciences, Inc. was founded in 1997 with the sole purpose of producing mammalian-derived recombinant cytokines. Today, we have drastically expanded our services to offer cytokine-measuring bioassays, in vitro anti-cancer and anti-inflammation lead drug development, as well as the... Show more »

SBH Sciences is a preclinical Contract Research Organization with extensive products and services of 345 human cancer cell lines, 300 bioassays, 31 recombinant cytokines, 8 recombinant Glycosyltransferase enzymes, 33 monoclonal antibodies, development of 30 ELISA kits and Multiplex analyses.

SBH Sciences is the world leader in cell-based assay to measure biological activity of cytokines and Growth Factors, and has provided services to over 150 biotechnology companies across the globe in our 18 years of business. Our proprietary processes have achieved commercial production of cytokines, new enzymes and monoclonal antibodies.

SBH Sciences, Inc. was founded in 1997 with the sole purpose of producing mammalian-derived recombinant cytokines. Today, we have drastically expanded our services to offer cytokine-measuring bioassays, in vitro anti-cancer and anti-inflammation lead drug development, as well as the development of ELISA kits and Multiplex analyses.

In the last few years, we have continued our diversification and through partnerships with several companies, our product line now includes specific enzymes and in vivo cancer and NASH models. We also expanded drastically our biomarker analysis capabilities and we are currently offering 8 platforms (ELISA, Luminex, PeggySue and WES, ELLA, FACS, qRT-PCR, Fluorescence Polarization and TR-FRET).

Our services have helped speed up the process of discovery and biopharmaceutical development by efficiently and effectively using our resources and expertise. Our services are customized and tailored to meet your company's specific needs. We provide time- and cost-saving processes that will give your company a competitive edge in the industry.

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qPCR
Quantitative PCR
Price on request

Our laboratory utilizes the Applied Biosystems StepOne PlusTM Real-Time PCR system. We have extensive experience designing and performing qPCR assays using both TaqMan and SYBR green detection chemistries.

Our qPCR workflow involves RNA extraction and QC, cDNA conversion, primer design and validation (if necessary), reference... Show more »

Our laboratory utilizes the Applied Biosystems StepOne PlusTM Real-Time PCR system. We have extensive experience designing and performing qPCR assays using both TaqMan and SYBR green detection chemistries.

Our qPCR workflow involves RNA extraction and QC, cDNA conversion, primer design and validation (if necessary), reference gene optimization and data analysis.

Our SYBR Green primer design and validation service involves optimization for amplicon size and primer specificity using both gel electrophoresis and melting curve analysis. We also perform an analysis of the efficiency of amplification for our designed primer sets.

Our gene expression analysis generates a report detailing differential gene expression across the dataset using the comparative Ct methodology between control and treated samples.

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RNA Extraction
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SBH Sciences is proud to introduce RNA isolation services for cultured cells and a variety of tissues. We have experience with both column-based and phenol/chloroform extraction methodologies. Our extraction service involves a full QC report detailing RNA concentration and purity via NanoDrop 2000c absorbance as well as assaying RNA integrity by gel electrophoresis.

SBH Sciences is proud to introduce RNA isolation services for cultured cells and a variety of tissues. We have experience with both column-based and phenol/chloroform extraction methodologies. Our extraction service involves a full QC report detailing RNA concentration and purity via NanoDrop 2000c absorbance as well as assaying RNA integrity by gel electrophoresis.

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Kinase Assays
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SBH Sciences offers cell-based kinase assay testing services utilizing ClariCELL™ assay technology from Cell Assay Innovations. The assays are designed and rigorously validated to be specific for a single kinase/substrate modification, yielding only hits that inhibit the specific phosphorylation event under study. Hence, they are... Show more »

SBH Sciences offers cell-based kinase assay testing services utilizing ClariCELL™ assay technology from Cell Assay Innovations. The assays are designed and rigorously validated to be specific for a single kinase/substrate modification, yielding only hits that inhibit the specific phosphorylation event under study. Hence, they are useful for screening, mechanism-of-action studies, and to establish direct inhibition of a particular kinase in cells.

Key features of the ClariCELL™ kinase cell-based assay testing service:

  • Specific measurement of particular kinase/protein substrate phosphorylation events
  • Value for primary screening, lead optimization, selectivity assessment, potency determinations, mechanism of action studies, and safety assessment
  • Broad coverage of the kinome, including tyrosine and serine/threonine kinase targets
  • Full length human kinases expressed in human cells
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Biologic/Biosimilar Development
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The patent expiration of over 20 blockbuster biologics in the next 10 years plus a combination of easier / newer FDA regulations will lead to significant opportunities for biosimilars and follow-on biologics. SBH Sciences is in a privileged position to assist companies developing biologicals, including monoclonal antibodies such... Show more »

The patent expiration of over 20 blockbuster biologics in the next 10 years plus a combination of easier / newer FDA regulations will lead to significant opportunities for biosimilars and follow-on biologics. SBH Sciences is in a privileged position to assist companies developing biologicals, including monoclonal antibodies such as anti-TNF-alpha and anti-VEGF and recombinant proteins like Interferon's, Erythropoietin's (EPO) and G-CSF agents.

SBH Sciences has the expertise needed to speed-up and implement accurate and reproducible bioassays that will be the essential component of the final product release. In addition, we can assist in lead drug development, including cell-culture, protein purification, ELISA-Based assay, analytical HPLC, formulation and stability studies, as well as drug delivery studies.

Please contact us so we can discuss your specific needs, and ways in which SBH Sciences might be able to work with you. We will be happy to assist you.

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In vitro Inflammation Models
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SBH Sciences is offering an In-Vitro inflammation model. The model is based on utilization of PBMC and the secretion of human TNF-alpha as a result of LPS stimulation. This model already been used by several companies.

Please contact us with your specific needs or any questions related to In-Vitro Inflammation investigation and anti-inflammation lead drug optimization.

SBH Sciences is offering an In-Vitro inflammation model. The model is based on utilization of PBMC and the secretion of human TNF-alpha as a result of LPS stimulation. This model already been used by several companies.

Please contact us with your specific needs or any questions related to In-Vitro Inflammation investigation and anti-inflammation lead drug optimization.

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Custom Cell-Based Assays
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SBH Sciences provides complete custom bioassay development for the Pharmaceutical, Biotechnology and diagnostic industry. Our experience in bioassay development allows us to quickly develop custom assays to fit most applications.

Whenever you need to analyze the activity of novel compounds or neutralization capability of your... Show more »

SBH Sciences provides complete custom bioassay development for the Pharmaceutical, Biotechnology and diagnostic industry. Our experience in bioassay development allows us to quickly develop custom assays to fit most applications.

Whenever you need to analyze the activity of novel compounds or neutralization capability of your antibody or receptor, please contact us. SBH Sciences can design, develop and validate the assay to meet your specific requirements.

Our in-vitro bioassays services will assist to assess the mechanism of action for monoclonal antibodies, recombinant proteins, small molecule therapeutics across a range of disease states including cardiovascular, inflammation, immunology, oncology, and CNS disorders.

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Biopharmaceutical Manufacturing
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Our fifteen years of operations have established our exceptional capabilities in techniques that may be of critical importance to scientists and companies in the fields of protein biology and therapeutics. We welcome inquiries from those seeking a dependable resource and collaborator in developing pharmaceutical and... Show more »

Our fifteen years of operations have established our exceptional capabilities in techniques that may be of critical importance to scientists and companies in the fields of protein biology and therapeutics. We welcome inquiries from those seeking a dependable resource and collaborator in developing pharmaceutical and biotechnology-derived products including:

  • Antibiotics
  • Recombinant proteins
  • Monoclonal antibodies

We specialize in

  • Cell culture, both mammalian and insect cells
  • Development of cell-based bioassays
  • Manipulation of existing cell lines to achieve substantial increases in expression
  • Protein Purification

Our proprietary processes enable us to achieve commercial production of highly purified recombinant cytokines and monoclonal antibodies.

SBH Sciences' technology is applicable to many areas of new drug discovery and pre clinical development, and we welcome the opportunity to discuss providing access to our technology through licensing, collaboration and/or outsourcing.

Some of the innovative capabilities SBH Sciences can offer fall within the following fields:

  • Cellular Biology (e.g., cloning of interest cDNA in a suitable vector, transient and stable mammalian cell transfection, select clones and demonstrate expression).
  • Cell Culture (e.g., serum-free media optimization, adaptation to suspension, production of cell lysates).
  • Protein Recovery and Purification (e.g., optimizing cross flow filtration, streamline chromatography, eliminate solvent utilization).
  • Analytical assay (e.g., HPLC, ELISA).
  • Cell-Based assay (e.g., proliferation and neutralization bioassay, agonist and antagonist screening).
  • Mid-scale production and purification of drug substance for pre-clinical study.

Working with us at SBH Sciences offers several key elements to improve your outcomes:

  • Streamlining of your process
  • Significant reduction of development time
  • Increasing yield while maintaining exceptional purity of the final product

The combination of these should assure a significant reduction in product cost.

We have capability to work with cells in roller bottles and in suspension in volumes up to 10L in continuous operation. We have developed in-house over twenty cell-culture-derived products, and have established ourselves as a premier supplier of high quality reagents for the R & D market.

We are open to any business model, including

Pure outsourcing (contract work)
A risk-shared model with appropriate reward-sharing possibilities
Other models where appropriate.

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Biochemical Assay Kits
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Cancer Cell Line Panels
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Cytokine Analysis
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Our cytokine bioassay capability is the most comprehensive one in the US and it is available for you. We are currently offering 300 bioassays in our two state-of-the-art cell culture facilities. SBH Sciences is pleased to offer to the scientific community a unique service, Cytokine Bioassays including: Proliferation and... Show more »

Our cytokine bioassay capability is the most comprehensive one in the US and it is available for you. We are currently offering 300 bioassays in our two state-of-the-art cell culture facilities. SBH Sciences is pleased to offer to the scientific community a unique service, Cytokine Bioassays including: Proliferation and Neutralization, In Vitro Cytotoxicity, Complement Dependent Cytotoxicity (CDCC), Agonist and Antagonist Screening as well as Cytokine Release Assay. In addition, we are continually developing new assays and would be pleased to add others upon your request. We can assure strict confidentiality and superior execution which will be delivered on a timely basis and cost effective manner. The expected turnover time per assay is less than two weeks, and a written report including methods, results, and conclusions will be issued. SBH Sciences is determined to maintain the highest standards and deliver the highest quality products and services. SBH Sciences welcomes suggestions from researchers and encourage you to communicate with us about your particular research needs.

Most of the bioassays are based on a colorimetric method of measuring cell activity using a non-radioactive substrate. This assay has been proven to be equivalent to the [3H]thymidine incorporated assay (Cell Signaling Applications Guide, Promega Corporation). The compound and the standard (if required) are reconstituted, serial diluted, and incubated with the cells over a 20 to 96 hour period.

BMPs (bone morphogenetic proteins) belong to the large group of growth and differentiation factors from the TGF-beta superfamily. The bioassay of the BMPs is based on measurement of alkaline phosphatase production by ATDC-5 chondrogenic cells.

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Multi-Analyte Profiling
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SBH Sciences is pleased to offer Multiplex Cytokines Custom Development and Testing Services based on our Cytokine expertise and the Luminex® xMAP Technology. xMAP technology allows simultaneous quantification of MAP, including cytokines / chemokines / biomarkers in cell culture and biological samples.

Testing services - We... Show more »

SBH Sciences is pleased to offer Multiplex Cytokines Custom Development and Testing Services based on our Cytokine expertise and the Luminex® xMAP Technology. xMAP technology allows simultaneous quantification of MAP, including cytokines / chemokines / biomarkers in cell culture and biological samples.

Testing services - We offer MAP testing services based on already available commercial assays, for many applications including: Cancer Markers, Cardiac Markers, Growth Factors, and Transcription Factors.fig3

Multiplexed bioassays - We provide cell-based bioassays in combination with MAP. Drug efficacy and dosage can be determined based on profiles of up to 50 analytes, utilizing our bioassay expertise and more than 200 in-house cell lines.

Custom bead development - No kit available for your biomarker? We can conjugate your antibodies of choice to build custom MAP kits. We also have experience in bead-peptide conjugation to screen for autoantibodies or protein binding partners. We will develop the assay, including standards, and evaluate, verify and optimize the dynamic range, signal linearity, assay specificity and will ensure Multiplex compatibility.

Our custom assays can be tailor-made for many applications including: Cancer Markers, Cardiac Markers, Growth Factors and Transcription Factors. It might assist to assess the mechanism of action for monoclonal antibodies, recombinant proteins, small molecule therapeutics across a range of disease states including cardiovascular, inflammation, immunology, oncology, and CNS disorders.

Biomarker production - Our expertise in the production and purification will be applied as well. SBH Sciences can assist you in the isolation of specific biomarkers from natural sources or production as recombinant proteins, while simultaneously developing your specific MultiAnalyte Method.

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VEGF Signaling Assays
Vascular Endothelial Growth Factor Assays
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CD20 Assays
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Angiopoietin-2 Assay
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Cytokine Release Assay
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In vitro Angiogenesis Assays
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We have developed three bioassays that are based on Tube Formation Assay: h-VEGF-alpha, h-GRO and h-IP-10.

We have developed three bioassays that are based on Tube Formation Assay: h-VEGF-alpha, h-GRO and h-IP-10.

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ELISA
Enzyme-linked immunosorbent assay
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We have developed over 20 highly sensitive and accurate colorimetric sandwich ELISA Kits for the measurement of Cytokines and Biomarkers. Our ELISA Kits are already distributed worldwide.

Partial list of our kits is including the following cytokines (e.g., TNF-a, VEGF, IL-12 p70), Biomarkers / Cancer (e.g., Galectin-1,... Show more »

We have developed over 20 highly sensitive and accurate colorimetric sandwich ELISA Kits for the measurement of Cytokines and Biomarkers. Our ELISA Kits are already distributed worldwide.

Partial list of our kits is including the following cytokines (e.g., TNF-a, VEGF, IL-12 p70), Biomarkers / Cancer (e.g., Galectin-1, Galectin-3) and apoptosis markers (e.g., Bcl-2, Caspase-3).

We are providing custom service that will meet your specific needs. Moreover, we are providing in-house optimization to verify the impact of various serum / plasma / cell culture on your PK/PD study.

We will develop and provide a comprehensive instruction manual for performing the assay. It will include all the steps including: coating and wash solutions, blocking and secondary (conjugated) antibody. If requested, we can also perform all the testing of your samples at our facility.

Our expertise in the production of recombinant proteins and monoclonal antibodies (MAb) will enable us to produce your desired antigen and MAb and will significantly reduce the cost associated with the production / reagents. In addition, we will optimize the capture and detection antibodies to allow lower detection limit and high precision and accuracy measurement.

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Cytotoxicity Assays
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We currently have over 400 mammalian cells with over 350 different human cancer cell lines, as well as numerous Primary human cells (e.g., HEKa, HUVEC, HMVEC, NHDF, NHEK, PBMC). We already perform thousands of anticancer drug testing. We can analyze the biological activity of your compound by itself or in combination with known... Show more »

We currently have over 400 mammalian cells with over 350 different human cancer cell lines, as well as numerous Primary human cells (e.g., HEKa, HUVEC, HMVEC, NHDF, NHEK, PBMC). We already perform thousands of anticancer drug testing. We can analyze the biological activity of your compound by itself or in combination with known anticancer drug. See below for a full list of anti-cancer drugs currently available.

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Neurotoxicity Testing
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In vitro Hepatotoxicity Testing
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Formulation Services
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ADCC and CDC Assay Services
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Antibodies mark invader cells for destruction in the pathway termed Antibody Dependent Cellular Cytotoxicity (ADCC), which comprises part of the adaptive immune response. After an antibody coats the target cell surface effector cells, such as natural killer (NK) cells, bind the Fc portion of the antibody, causing the Fc receptors... Show more »

Antibodies mark invader cells for destruction in the pathway termed Antibody Dependent Cellular Cytotoxicity (ADCC), which comprises part of the adaptive immune response. After an antibody coats the target cell surface effector cells, such as natural killer (NK) cells, bind the Fc portion of the antibody, causing the Fc receptors to be cross-linked and activating the effector cell.

In vitro ADCC assays are the first step in evaluating the therapeutic potential of an antibody. SBH Sciences now offers ADCC assay services, based upon the Promega ADCC Reporter Bioassay™ technology.

Traditional ADCC assays require the use of donor peripheral blood mononuclear cells (PBMCs) as effector cells, and typically monitor apoptosis by measuring release of a radioactive marker. PBMC variability can lead to inconsistent results, and use of radioactive markers complicates safety considerations. The Promega ADCC Reporter Bioassay™ uses an engineered effector cell that stably expresses the FcγRIIIa receptor, which is coupled to expression of luciferase. Upon addition of the Bio-Glo™ substrate, activated effector cells luminesce. No donor cells or radioactive markers are needed.

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Receptor Binding Models
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In vivo Protein Binding Assays
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Elisa Kit Development
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SBH Sciences provides Contract ELISA Development Kit services for the R&D and IVD community. We have developed over 20 highly sensitive and accurate colorimetric sandwich ELISA Kits for the measurement of Cytokines and Biomarkers. Our ELISA Kits are already distributed worldwide.

Partial list of our kits is including the... Show more »

SBH Sciences provides Contract ELISA Development Kit services for the R&D and IVD community. We have developed over 20 highly sensitive and accurate colorimetric sandwich ELISA Kits for the measurement of Cytokines and Biomarkers. Our ELISA Kits are already distributed worldwide.

Partial list of our kits is including the following cytokines (e.g., TNF-a, VEGF, IL-12 p70), Biomarkers / Cancer (e.g., Galectin-1, Galectin-3) and apoptosis markers (e.g., Bcl-2, Caspase-3).

We are providing custom service that will meet your specific needs. Moreover, we are providing in-house optimization to verify the impact of various serum / plasma / cell culture on your PK/PD study.

We will develop and provide a comprehensive instruction manual for performing the assay. It will include all the steps including: coating and wash solutions, blocking and secondary (conjugated) antibody. If requested, we can also perform all the testing of your samples at our facility.

Our expertise in the production of recombinant proteins and monoclonal antibodies (MAb) will enable us to produce your desired antigen and MAb and will significantly reduce the cost associated with the production / reagents. In addition, we will optimize the capture and detection antibodies to allow lower detection limit and high precision and accuracy measurement.

Please contact us so we can discuss your specific needs, and ways in which SBH Sciences might be able to work with you. We will be happy to assist you.

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Therapeutic Monoclonal Antibody (mAb) Development
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Nucleic Acid Services
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Enzyme Assays
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Cells and Tissues
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Biomolecular Interaction Analysis
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Biomarkers
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Protein Quantification
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Protein Services
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Toxicology
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Biology
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Angiogenesis & Endothelial Cell Assays
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Bioanalysis
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Antibody Services
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ELISA Kits
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RNA
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Biochemical Assays
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In vitro Toxicity Testing
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In vitro Disease Models
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Specialized Cell-Based Assays
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Cell-Based Assays
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Manufacturing Services
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Cell Lines
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Computational Modeling
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Biochemistry & Molecular Biology
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Pharmacology & Toxicology
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Drug Discovery & Development
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PCR
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Cell Signaling Analyses
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Immunoassays
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DNA
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Drug Development
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