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SARomics Biostructures AB

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Lund, SE

About SARomics Biostructures AB

SARomics Biostructures is a technology driven research company located in the Medicon Valley region of southern Sweden. Our platform is focused around state-of-the-art industry leading technologies for high-throughput protein crystallization and structure determination, computational chemistry and fragment-based screening. Our expertise in structure-based design is applied to a number of innovative early stage drug discovery projects as well as fee for service projects to clients. Our extensive portfolio of FastLane off-the-shelf protein structures enables faster project execution that substantially accelerates the drug discovery process. Our close proximity to one of the world's most advanced synchrotron light sources, the MAX IV Laboratory, provides us with extremely rapid access to synchrotron beamtime, enabling our highly experienced team to deliver results at a competitive price with turn-around times of 2-8 weeks.

Our Services (8)


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Fragment Screening

Price on request

The integrated drug discovery and structure-based drug design (SBDD) platform at SARomics Biostructures offers fragment screening services, which include hit identification and hit-to-lead optimization for any target with known three-dimensional structure. Our extensive expertise in screening, computational chemistry and structure-based drug design is combined with the expertise of our in-house collaboration partner Red Glead Discovery to provide full-range discovery services including hit-to-lead optimization, assay development, medicinal chemistry and ADME.


WAC™ screening technology - Our proprietary weak affinity chromatography (WAC™) screening technology allows simultaneous identification of weak binders (mM-μM) in complex mixtures, as well as Kd calculations. Offer packages include Pilot screen, Fragment screen and Ligandability screen. Our clients have the choice between our proprietary fragment library designed for easy hit expansion, a specifically ordered sets or client libraries. 


We may also perform fragment screening by other biophysical methods where we perform efficient hit identification using NMR spectroscopy and/or X-ray crystallography.


Computational chemistry - Library design, hit expansion, pharmacophore- and shape-based virtual screening, scaffold hopping, QSAR analysis and optimization.


Structural biology - Highly efficient structural biology platform with proprietary high-throughput crystallization and structure determination protocols.


Medicinal chemistry - Through our collaboration with our inhouse partner Red Glead Discovery we offer expertise in lead optimization, biological characterization, medicinal chemistry and ADME.

Fragment screening Drug Discovery

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X-ray Crystallography

Price on request

SARomics Biostructures crystallography services include

  • Custom Gene-to-Structure services, including cloning, expression, purification and characterization of a protein using biophysical methods, followed by crystallization and high resolution X-ray structure determination. 
  • FastLane™ Premium and FastLane™ Standard libraries of verified drug target proteins (off-the-shelf structures). The libraries include a large number of kinases, phosphatases, epigenetic targets and other proteins.
  • Antibody and Antibody-Antigen Crystal Structures.

Our contract crystallization and X-ray structure determination services has in most cases a turnaround time of 4 to 8 weeks. 


A number of proteins from the FastLane™ Premium library can also be found in our catalogue of high purity crystallization grade recombinant proteins. 


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Drug Discovery

Price on request

The integrated drug discovery and structure-based drug design (SBDD) platform at SARomics Biostructures offers fragment screening services, which include hit identification and hit-to-lead optimization for any target with known three-dimensional structure. Our extensive expertise in screening, computational chemistry and structure-based drug design is combined with the expertise of our in-house collaboration partner Red Glead Discovery to provide full-range discovery services including hit-to-lead optimization, assay development, medicinal chemistry and ADME.


Our services include:


- WAC™ screening technology - Our proprietary weak affinity chromatography (WAC™) screening technology allows simultaneous identification of weak binders (mM-μM) in complex mixtures, as well as Kd calculations. Offer packages include Pilot screen, Fragment screen and Ligandability screen. Our clients have the choice between our proprietary fragment library designed for easy hit expansion, a specifically ordered sets or client libraries. Please enquire for details.


- Biophysical screening - Efficient hit identification using thermal-shift assay (DSF), isothermal titration calorimetry (ITC), NMR spectroscopy and X-ray crystallography.


- Computational chemistry - Library design, hit expansion, pharmacophore- and shape-based virtual screening, scaffold hopping, QSAR analysis and optimization.


- Structural biology - Highly efficient structural biology platform with proprietary high-throughput crystallization and structure determination protocols.


- Medicinal chemistry - Through our collaboration with our inhouse partner Red Glead Discovery we offer expertise in lead optimization, biological characterization, medicinal chemistry and ADME.


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Computational Chemistry

Price on request

Library design, hit expansion, pharmacophore- and shape-based virtual screening, scaffold hopping, QSAR analysis and optimization.


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Structural Biology

Price on request

Highly efficient structural biology platform with proprietary high-throughput crystallization and structure determination protocols.



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Medicinal Chemistry

Price on request

Through our collaboration with our inhouse partner Red Glead Discovery we offer expertise in lead optimization, biological characterization, medicinal chemistry and ADME.


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NMR

Nuclear magnetic resonance spectroscopy
Price on request

SARomics Biostructures offers a broad range of custom protein nuclear magnetic resonance (NMR) spectroscopy services for protein three-dimensional structure determination. Among our services are the following:


- Protein structure determination in solution

- Binding assays/fragment screening

- Identification of flexible regions

- Verification of the structural integrity of target protein constructs

- pKa determination of titrating groups

- Validation of peptides and other low-molecular-weight compounds


High-resolution 3D structure determination in solution


In structural biology, as an alternative to protein structure determination by X-ray crystallography, it is possible to use NMR spectroscopy to study protein structure and dynamics in solution. Although the resolution of the NMR data is normally lower than that of X-ray crystallographic data, structure determination by NMR spectroscopy may still provide valuable information on the structure, its dynamics, ligand binding sites (ligand screening), etc. One obvious case is when a highly flexible protein resists crystallization, but may still yield high-quality NMR spectra.


Ligand binding assay and fragment screening


Which ligands in your panel bind to the target protein? Where in the protein is the binding site located? What is the dissociation constant for a particular ligand? NMR experiments offer convenient means of addressing these questions and many more.


Verification of target constructs


Does your construct result in a folded, well-behaved protein? Protein NMR can provide a high-resolution picture of the physical state of a protein. The HSQC fingerprint spectrum readily shows whether the protein is well-folded, unfolded or ‘molten globule-like, and whether parts of the protein are flexible. This information is often very useful as a preparatory step before initiating crystallization trials.


pKa determination of titrating groups


Knowing the pKa values of amino acid residues in the vicinity of the binding/active site of a biological macromolecule can be crucial for accurate modeling of the electrostatic interactions that contribute to ligand binding and consequently for computational optimization of designed ligands and in silico screening. NMR spectroscopy is the only experimental technique available that enables atom-specific information of protonation equilibria in proteins.


Validation of peptides and other low-molecular-weight compounds


Is the primary sequence of your peptidomimetic lead what you expect it to be? Does your peptide populate multiple conformations in solution? Which one is the active one? NMR spectroscopy is a very powerful tool for investigating these types of problems. We perform multi-dimensional (1H/1H, 1H/13C, or 1H/15N) NMR experiments as a basis for detailed structure analysis.


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Protein Expression Screening and Optimization

Price on request

ProPHECY™ - in silico Optimization


By using the ProPHECY™ method you will:


• Reduce the effort, time and costs involved in optimizing one or several targeted protein or peptide properties

• Reach your goals faster and at lower cost compared to random screening

• Discover hidden information in large and complex data sets

• Predict the effect of a single amino acid replacement from data containing complex replacement patterns

• Achieve detailed quantitative information on the influence of each amino acid replacement

• Perform parallel optimization of several parameters

• Understand sequence-activity-relationships for your target protein or peptide

• Use the gained information to rationally predict and design new optimized variants


About the method


It is often desirable to modify a biomolecule (peptide, polypeptide, protein, antibody etc.) to optimize specific properties in a rational way. For example:


• increase its biological or catalytic activity

• decrease its antigenicity

• enhance its cell penetrating capabilities

• protease resistance,

• thermal stability,

• solubility


Our proprietary and proven ProPHECY™ in silico protein optimization technology offers a quick and less costly approach.


By modifying and optimizing the amino acid sequence of a protein one could explore the structure-activity relationships (SAR) and using them control protein properties and eventually modify them in a desired way. ProPHECY™ is reminiscent of QSAR (Quantitative Structure-Activity Relationship) methodology used for decades in the optimization of organic small molecules. It uses a novel method by which experimentally determined properties of a protein can be utilized for the prediction of amino acid replacements required to achieve controlled modifications. This will greatly reduce effort, time and costs required in a protein optimization project. You are welcome to contact us for further discussion of your project.


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