Sanquin Diagnostiek B.V.
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Amsterdam, NL
Blood is life
We are the only organization in the world that offer in-house medical, pharmaceutical and scientific knowledge and expertise, all under one roof. With almost 3,000 committed colleagues and 350,000 blood donors, we provide a better life for 300,000 patients each year.
T cells can be activated in vitro with anti-CD3/anti-CD28, PMA/Ionomycin or Super Antigen stimulation. Alternatively, T cells can be co-cultured with DCs and stimulated in an antigen-specific manner (e.g. Tetanus toxoid).
Read-out includes proliferation with 3H thymidine or fluorescent proliferation dyes, as well as cytokine production and expression of activation markers.
This human whole blood basophil activation assay is well suited for small sample sizes. The basophils are activated in a physiological environment, and the flow cytometry read-out ensures quick and accurate results.
After isolation and expansion of regulatory T cells, their capacity to suppress proliferation of conventional T cells can be analyzed within allogeneic PBMC cultures. The suppression of CD4+ and CD8+ T cell proliferation can be measured by flow cytometry.
This method of quantification enables analysis of up to 30 different cytokines, chemokines or growth factors in a single sample.
With our broad knowledge in immunology, we can offer our expertise in many types of cell-based assays. Whether it is setting up an all new assay or adjusting an existing assay to fit your needs.
Many cell-based assays are available to perform compounds screens. Examples: proliferation assays, cytokine analysis, whole blood stimulation and Mixed Lymphocyte Reactions.
Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).
Bloodtype genotyping (including allelic discriminations, clotting diseases and red cell deficiencies)
Leukemia markers/ gene expression (eg, p190, bcr, braf, myd88 etc)
HLA typing (low- and high resolution using NGS)
Nucleic extractions (DNA and RNA)
We are pleased to perform pyrogen testing with MAT on your product batches
- Our laboratory is GMP and ISO 15189 certified
- MAT is executed according to European Pharmacopeia chapter 2.6.30 (Method A, B and/or C)
- Potential pyrogen contamination will be quantified relative to an international standard of endotoxin
- Experienced in MAT testing of proteins and antibodies from human and recombinant origin
Based on customer needs we perform product-specific validations. We also provide MAT feasibility testing, method development, SOP writing and in-process/batch release testing to reduce the workload of your laboratory. The customer will receive either a result letter, for in-process/batch release testing, or a (validation) report describing the performed tests and the obtained results.
Description of the assay:
General design of the assay:
Additional assays/ expertise:
Besides the NK cell cytotoxicity assays, we have several assay set-ups available to determine activation, proliferation and differentiation of immune cells, mainly using flow cytometry as a read-out. We generally focus on complete peripheral blood mononuclear cells or isolated T cells for these type of analyses, but these can of course also be applied to NK cells.
This human whole blood B cell activation assay is well suited for small sample sizes. The B cells are activated in a physiological environment, and the flow cytometry read-out ensures quick and accurate results.
This assay can be performed as a one-way MLR by co-culturing irradiated PBMCs from one donor with untreated PBMCs from another donor, or as a two-way MLR through co-culture of PBMCs from two untreated donors. It is also possible to isolate T cells from a donor before co-culture and use those for read out of proliferation. Proliferation can be monitored through incorporation of 3H thymidine, or, when more detailed information is required, can be measured by combining a FACS staining with a fluorescent proliferation dye.
We have a state-of-the-art flow cytometry facility and can offer fully customizable, elaborate multi-colour flow cytometry panels to allow in-depth phenotyping of any type of immune cell.
Biomarkers Services
Biology Services
Clinical Trials, Consulting, and Management Services
Pharmacology & Toxicology Services
Clinical Immunology Tests Services
Blood Banking and Transfusion Medicine Services
Clinical Hematology Services
Clinical Laboratory Services
Clinical Research Services
Immunoassays Services
Bioanalytical Assays Services
eg. Selection CD8+ T-cell epitopes, antigen-specific cytotoxic T-cell monitoring and On/ off target screening.
Antibody Services
We can perform proliferation assays using 3H thymidine incorporation and/or proliferation dyes.
Proliferation of PBMCs, total T cells, CD4+ T cells, CD8+ T cells, NK cells and B cells or a combination thereof can be monitored.
Cell viability can be monitored by flow cytometry.
Testing of Cell Lines Services
Cells and Tissues Services
Protein Quantification Services
Protein Purification and Quantification Services
Protein Services
Biochemistry & Molecular Biology Services
Functional & Cell Type Specific Assays Services
Gene Panel Testing Services
Clinical Molecular Diagnostics Services
Nucleic Acid Services
Mesenteric vessel staining
Isolate, fix and staining of vessels
Migration- and Transmigration assays
Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).
Cell Permeability Assay - leakage assay
Using an epithelial cell line monolayer in a transwell system.
Pharmacology Services
Cell Death Assays Services
Drug Discovery & Development Services
Cell Invasion & Migration Assays Services
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