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Sanquin Diagnostiek B.V.

20 Orders Completed
Amsterdam, NL

About Sanquin Diagnostiek B.V.

Blood is life

We are the only organization in the world that offer in-house medical, pharmaceutical and scientific knowledge and expertise, all under one roof. With almost 3,000 committed colleagues and 350,000 blood donors, we provide a better life for 300,000 patients each year.

Our Services (61)


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T Cell Activation and Proliferation Assays

Price on request

T cells can be activated in vitro with anti-CD3/anti-CD28, PMA/Ionomycin or Super Antigen stimulation. Alternatively, T cells can be co-cultured with DCs and stimulated in an antigen-specific manner (e.g. Tetanus toxoid).

Read-out includes proliferation with 3H thymidine or fluorescent proliferation dyes, as well as cytokine production and expression of activation markers.


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DNA Extraction and Purification

Price on request
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Basophil Activation Test (BAT)

Price on request

This human whole blood basophil activation assay is well suited for small sample sizes. The basophils are activated in a physiological environment, and the flow cytometry read-out ensures quick and accurate results.


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Functional T-cell Suppression Assays

Price on request

After isolation and expansion of regulatory T cells, their capacity to suppress proliferation of conventional T cells can be analyzed within allogeneic PBMC cultures. The suppression of CD4+ and CD8+ T cell proliferation can be measured by flow cytometry.

Autoimmunity Immuno-Oncology BD FacsCantoII/LSRII Flow cytometry PBMC's CD4+ T cells Human Show 7 more tags Show less

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Cytometric Bead Array (CBA)

Price on request

This method of quantification enables analysis of up to 30 different cytokines, chemokines or growth factors in a single sample.

BD FacsCantoII/LSRII Flow cytometry Human

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Custom Cell-Based Assay Development

Price on request

With our broad knowledge in immunology, we can offer our expertise in many types of cell-based assays. Whether it is setting up an all new assay or adjusting an existing assay to fit your needs.


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Cell-Based Compound Screening

Price on request

Many cell-based assays are available to perform compounds screens. Examples: proliferation assays, cytokine analysis, whole blood stimulation and Mixed Lymphocyte Reactions.


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AH50 Assay

Price on request

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).


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Genotyping and Gene Expression Assays

Price on request

Bloodtype genotyping (including allelic discriminations, clotting diseases and red cell deficiencies)

Hematology

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Leukemia Gene Panel

Price on request

Leukemia markers/ gene expression (eg, p190, bcr, braf, myd88 etc)

Hematology

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HLA Typing

Price on request

HLA typing (low- and high resolution using NGS)


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RNA Extraction and Purification

Price on request

Nucleic extractions (DNA and RNA)


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Monocyte Activation Testing

Price on request

We are pleased to perform pyrogen testing with MAT on your product batches
- Our laboratory is GMP and ISO 15189 certified
- MAT is executed according to European Pharmacopeia chapter 2.6.30 (Method A, B and/or C)
- Potential pyrogen contamination will be quantified relative to an international standard of endotoxin
- Experienced in MAT testing of proteins and antibodies from human and recombinant origin

Based on customer needs we perform product-specific validations. We also provide MAT feasibility testing, method development, SOP writing and in-process/batch release testing to reduce the workload of your laboratory. The customer will receive either a result letter, for in-process/batch release testing, or a (validation) report describing the performed tests and the obtained results.


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ELISpot Assays

Enzyme-Linked ImmunoSpot Assays
Price on request
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Cytokine Analysis

Price on request
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Natural Killer Cell Cytotoxicity Assay

Price on request

Description of the assay:

  1. Effector cells (E):
    a. positive (CD56+) selection of NK cells from fresh blood-isolated peripheral mononuclear blood cells (PBMC) using magnetic beads– preferred method of selection
    b. negative (all other cells but NK cells depleted from PBMC)
  2. Target cells (T):
    a. SKBR3 breast carcinoma cell line, positive for Her2/Neu, possible to opsonize with trastuzumab to perform antibody-dependent cellular cytotoxicity assay (ADCC)
    b. A431 colon carcinoma cell line, positive for EGFR, possible to opsonize with cetuximab to perform ADCC
    c. Raji Burkitt lymphoma cell line, positive for CD20, possible to opsonize with rituximab to perfrom ADCC
  3. Assay: target cells are labeled with radioactive 51Cr , and level of release is measured after incubation with NK cells, assay is performed in technical triplicate

General design of the assay:

  • Target cell lines of interest tested (can test all three cell lines, depending on the final design either all target cell lines in parallel or separately)
  • Test both spontaneous and ADCC (ADCC is about 2-fold higher than spontaneous cytotoxicity)
  • Test 6 different healthy individuals
  • Test different E:T ratio
  • Step 1: test one concentration of all n=x compounds in these conditions
  • Step 2: depending on the result, choose fixed E:T ratio and perform dose-dependency with n=x compounds

Additional assays/ expertise:
Besides the NK cell cytotoxicity assays, we have several assay set-ups available to determine activation, proliferation and differentiation of immune cells, mainly using flow cytometry as a read-out. We generally focus on complete peripheral blood mononuclear cells or isolated T cells for these type of analyses, but these can of course also be applied to NK cells.


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B Cell Activation Assay

Price on request

This human whole blood B cell activation assay is well suited for small sample sizes. The B cells are activated in a physiological environment, and the flow cytometry read-out ensures quick and accurate results.


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Mixed Lymphocyte Reactions

Price on request

This assay can be performed as a one-way MLR by co-culturing irradiated PBMCs from one donor with untreated PBMCs from another donor, or as a two-way MLR through co-culture of PBMCs from two untreated donors. It is also possible to isolate T cells from a donor before co-culture and use those for read out of proliferation. Proliferation can be monitored through incorporation of 3H thymidine, or, when more detailed information is required, can be measured by combining a FACS staining with a fluorescent proliferation dye.


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Immunophenotyping

Price on request

We have a state-of-the-art flow cytometry facility and can offer fully customizable, elaborate multi-colour flow cytometry panels to allow in-depth phenotyping of any type of immune cell.


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Cell-Based Assays

Price on request
Request a quote for more information about this service.

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Clinical PK/PD Studies

Price on request
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Bioanalysis

Price on request
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Clinical Immunogenicity Testing

Price on request
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Human Blood Sample Processing

Price on request
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Biomarkers

Price on request

Biomarkers Services


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Biology

Price on request

Biology Services


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Clinical Trials, Consulting, and Management

Price on request

Clinical Trials, Consulting, and Management Services


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Pharmacology & Toxicology

Price on request

Pharmacology & Toxicology Services


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Clinical Immunology Tests

Price on request

Clinical Immunology Tests Services


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Blood Banking and Transfusion Medicine

Price on request

Blood Banking and Transfusion Medicine Services


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Clinical Hematology

Price on request

Clinical Hematology Services


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Clinical Laboratory Services

Price on request

Clinical Laboratory Services


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Clinical Research

Price on request

Clinical Research Services


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Immunoassays

Price on request

Immunoassays Services


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Bioanalytical Assays

Price on request

Bioanalytical Assays Services


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Epitope Mapping

Price on request

eg. Selection CD8+ T-cell epitopes, antigen-specific cytotoxic T-cell monitoring and On/ off target screening.


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Antibody Services

Price on request

Antibody Services


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Cell Viability & Proliferation Assays

Price on request

We can perform proliferation assays using 3H thymidine incorporation and/or proliferation dyes.

Proliferation of PBMCs, total T cells, CD4+ T cells, CD8+ T cells, NK cells and B cells or a combination thereof can be monitored.


Cell viability can be monitored by flow cytometry.


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Testing of Cell Lines

Price on request

Testing of Cell Lines Services


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Cells and Tissues

Price on request

Cells and Tissues Services


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Protein Quantification

Price on request

Protein Quantification Services


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Protein Purification

Price on request

Protein Purification and Quantification Services


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Protein Services

Price on request

Protein Services


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Biochemistry & Molecular Biology

Price on request

Biochemistry & Molecular Biology Services


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Functional & Cell Type Specific Assays

Price on request

Functional & Cell Type Specific Assays Services


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Gene Panel Testing

Price on request

Gene Panel Testing Services


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Clinical Molecular Diagnostics

Price on request

Clinical Molecular Diagnostics Services


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Nucleic Acid Services

Price on request

Nucleic Acid Services


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Blood Vessel Staining

Price on request

Mesenteric vessel staining

Isolate, fix and staining of vessels


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Transwell Assay

Price on request

Migration- and Transmigration assays


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CH50 Assay

Price on request

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).


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Cell Permeability Assay

Price on request

Cell Permeability Assay - leakage assay

Using an epithelial cell line monolayer in a transwell system.


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DNA Sequencing

Price on request
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ELISA

Enzyme-linked immunosorbent assay
Price on request
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ELISA

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Pharmacology

Price on request

Pharmacology Services


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In vitro Immunogenicity Assays

Price on request
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Fluorescence Activated Cell Sorting (FACS)

Fluorescence Activated Cell Sorting
Price on request
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Human Immune Cell Assays

Price on request
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Cell Death Assays

Price on request

Cell Death Assays Services


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Drug Discovery & Development

Price on request

Drug Discovery & Development Services


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Cell Invasion & Migration Assays

Price on request

Cell Invasion & Migration Assays Services


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