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Sanquin Diagnostiek B.V.

Amsterdam, NL

Blood is of vital importance

We are the only organization in the world that can boast in-house medical, pharmaceutical and even scientific knowledge and expertise. With almost 3,000 committed colleagues and 350,000 blood donors, we can provide a better life for 300,000 patients each year.

Sanquin Diagnostiek B.V. has not listed any services.

AH50 Assay
Price on request

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).

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Genotyping Services
Price on request

Bloodtype genotyping (including allelic discriminations, clotting diseases and red cell deficiencies)

Bloodtype genotyping (including allelic discriminations, clotting diseases and red cell deficiencies)

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Hematology
Leukemia Gene Panel
Price on request

Leukemia markers/ gene expression (eg, p190, bcr, braf, myd88 etc)

Leukemia markers/ gene expression (eg, p190, bcr, braf, myd88 etc)

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HLA Typing
Price on request

HLA typing (low- and high resolution using NGS)

HLA typing (low- and high resolution using NGS)

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RNA Extraction and Purification
Price on request

Nucleic extractions (DNA and RNA)

Nucleic extractions (DNA and RNA)

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DNA Extraction
Price on request

Nucleic extractions (DNA and RNA)

Nucleic extractions (DNA and RNA)

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Cell Proliferation Assays
Price on request

We can perform proliferation assays based on 3H thymidine incorporation and/or proliferation dye.

We can perform proliferation assays based on 3H thymidine incorporation and/or proliferation dye.

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Monocyte Activation Testing
Price on request
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ELISpot Assays
Enzyme-Linked ImmunoSpot Assays
Price on request
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Cytokine Analysis
Price on request
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Natural Killer Cell Cytotoxicity Assay
Price on request

Description of the assay:
1. Effector cells (E):
a. positive (CD56+) selection of NK cells from fresh blood-isolated peripheral mononuclear blood cells (PBMC) using magnetic beads– preferred method of selection
b. negative (all other cells but NK cells depleted from PBMC)
2. Target cells (T):
a. SKBR3 breast carcinoma cell... Show more »

Description of the assay:
1. Effector cells (E):
a. positive (CD56+) selection of NK cells from fresh blood-isolated peripheral mononuclear blood cells (PBMC) using magnetic beads– preferred method of selection
b. negative (all other cells but NK cells depleted from PBMC)
2. Target cells (T):
a. SKBR3 breast carcinoma cell line, positive for Her2/Neu, possible to opsonize with trastuzumab to perform antibody-dependent cellular cytotoxicity assay (ADCC)
b. A431 colon carcinoma cell line, positive for EGFR, possible to opsonize with cetuximab to perform ADCC
c. Raji Burkitt lymphoma cell line, positive for CD20, possible to opsonize with rituximab to perfrom ADCC
3. Assay: target cells are labeled with radioactive 51Cr , and level of release is measured after incubation with NK cells, assay is performed in technical triplicate

General design of the assay:
- Target cell lines of interest tested (can test all three cell lines, depending on the final design either all target cell lines in parallel or separately)
- Test both spontaneous and ADCC (ADCC is about 2-fold higher than spontaneous cytotoxicity)
- Test 6 different healthy individuals
- Test different E:T ratio
- Step 1: test one concentration of all n=x compounds in these conditions
- Step 2: depending on the result, choose fixed E:T ratio and perform dose-dependency with n=x compounds

Additional assays/ expertise:
Besides the NK cell cytotoxicity assays, we have several assay set-ups available to determine activation, proliferation and differentiation of immune cells, mainly using flow cytometry as a read-out. We generally focus on complete peripheral blood mononuclear cells or isolated T cells for these type of analyses, but these can of course also be applied to NK cells.

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B Cell Activation Assay
Price on request
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Mixed Lymphocyte Reactions
Price on request
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Immunophenotyping
Price on request
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Cell-Based Assays
Price on request
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Clinical PK/PD Studies
Price on request
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Bioanalysis
Price on request
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Clinical Immunogenicity Testing
Price on request
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Human Blood Sample Processing
Price on request
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Biomarkers
Price on request

Biomarkers Services

Biomarkers Services

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Biology
Price on request

Biology Services

Biology Services

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Clinical Trials, Consulting, and Management
Price on request

Clinical Trials, Consulting, and Management Services

Clinical Trials, Consulting, and Management Services

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Pharmacology & Toxicology
Price on request

Pharmacology & Toxicology Services

Pharmacology & Toxicology Services

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Clinical Immunology Tests
Price on request

Clinical Immunology Tests Services

Clinical Immunology Tests Services

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Blood Banking and Transfusion Medicine
Price on request

Blood Banking and Transfusion Medicine Services

Blood Banking and Transfusion Medicine Services

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Clinical Hematology
Price on request

Clinical Hematology Services

Clinical Hematology Services

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Clinical Laboratory Services
Price on request

Clinical Laboratory Services

Clinical Laboratory Services

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Clinical Research
Price on request

Clinical Research Services

Clinical Research Services

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Immunoassays
Price on request

Immunoassays Services

Immunoassays Services

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Bioanalytical Assays
Price on request

Bioanalytical Assays Services

Bioanalytical Assays Services

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Epitope Mapping
Price on request

eg. Selection CD8+ T-cell epitopes, antigen-specific cytotoxic T-cell monitoring and On/ off target screening.

eg. Selection CD8+ T-cell epitopes, antigen-specific cytotoxic T-cell monitoring and On/ off target screening.

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Antibody Services
Price on request

Antibody Services

Antibody Services

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Cell Viability & Proliferation Assays
Price on request

Cell Viability & Proliferation Assays Services

Cell Viability & Proliferation Assays Services

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Testing of Cell Lines
Price on request

Testing of Cell Lines Services

Testing of Cell Lines Services

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Cells and Tissues
Price on request

Cells and Tissues Services

Cells and Tissues Services

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Protein Quantification
Price on request

Protein Quantification Services

Protein Quantification Services

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Protein Purification
Price on request

Protein Purification and Quantification Services

Protein Purification and Quantification Services

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Protein Services
Price on request

Protein Services

Protein Services

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Biochemistry & Molecular Biology
Price on request

Biochemistry & Molecular Biology Services

Biochemistry & Molecular Biology Services

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Immune Cell Assays
Price on request

Immune Cell Assays Services

Immune Cell Assays Services

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Functional & Cell Type Specific Assays
Price on request

Functional & Cell Type Specific Assays Services

Functional & Cell Type Specific Assays Services

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Gene Panel Testing
Price on request

Gene Panel Testing Services

Gene Panel Testing Services

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Clinical Molecular Diagnostics
Price on request

Clinical Molecular Diagnostics Services

Clinical Molecular Diagnostics Services

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RNA Services
Price on request

RNA Services

RNA Services

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DNA Extraction and Purification
Price on request

DNA Extraction and Purification Services

DNA Extraction and Purification Services

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DNA Services
Price on request

DNA Services

DNA Services

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Nucleic Acid Services
Price on request

Nucleic Acid Services

Nucleic Acid Services

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Blood Vessel Staining
Price on request

Mesenteric vessel staining

Isolate, fix and staining of vessels

Mesenteric vessel staining

Isolate, fix and staining of vessels

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Transwell Assay
Price on request

Migration- and Transmigration assays

Migration- and Transmigration assays

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CH50 Assay
Price on request

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).

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Cell Permeability Assay
Price on request

Cell Permeability Assay - leakage assay

Day 1
Seed epithelial cell line H292 in RPMI / 10%FCS / PS / L-glut
12WP transwell membrane
0.4 μm pore
12mm membrane
8.0•104 / well
RPMI / 10% FCS / 1% L-glutamin

Note/ It is not possible to view the forming monolayer through the membrane; therefore, seed a similar amount of... Show more »

Cell Permeability Assay - leakage assay

Day 1
Seed epithelial cell line H292 in RPMI / 10%FCS / PS / L-glut
12WP transwell membrane
0.4 μm pore
12mm membrane
8.0•104 / well
RPMI / 10% FCS / 1% L-glutamin

Note/ It is not possible to view the forming monolayer through the membrane; therefore, seed a similar amount of cells in a 24WP for comparison. The 12mm TW has a growth area of 1cm2; the wells from a 24WP have a growth area of 2cm2; seed two wells of a 24WP with 8.0•104 / well and 2 wells of a 24WP with 16•104 / well

Day 2 / 3
Refresh medium

Day 4
Remove phonol red:
Wash cells and bottom of TW with
Hepes containing 0.5% CeAlb
Transfer TW to new/clean 12WP

Add Dextran-Fluorescein 3000 and / or drugs
Allow dextrin-fluorescein to pass through membrane for 2 hrs
Measure λex 494 nm / λem 521 nm on an elisa plate reader
Magellan Fluorescein Standard: λex 485 nm / λem 535 nm

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Cell Invasion & Migration Assays
Price on request

Cell Invasion & Migration Assays Services

Cell Invasion & Migration Assays Services

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Drug Discovery & Development
Price on request

Drug Discovery & Development Services

Drug Discovery & Development Services

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Cell Death Assays
Price on request

Cell Death Assays Services

Cell Death Assays Services

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In vitro Immunogenicity Assays
Price on request
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Pharmacology
Price on request

Pharmacology Services

Pharmacology Services

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ELISA
Enzyme-linked immunosorbent assay
Price on request
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DNA Sequencing
Price on request
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2018-08-14 18:18:30 +0200

Net Promoter Score of 9 received for Natural Killer Cell Cytotoxicity Assay.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-06-05 18:15:50 +0200

Net Promoter Score of 9 received for Cell Proliferation Assays.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-06-05 18:15:22 +0200

Net Promoter Score of 9 received for Natural Killer Cell Cytotoxicity Assay.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-04-23 17:39:15 +0200

Net Promoter Score of 9 received for Natural Killer Cell Cytotoxicity Assay.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-04-23 17:38:27 +0200

Net Promoter Score of 9 received for Natural Killer Cell Cytotoxicity Assay.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.

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