48djuebysmcs9yomswoq sanquin

Sanquin Diagnostiek B.V.

ic 8.9/10 Overall Rating No Reviews Yet
ic 12 Orders Completed
Amsterdam, NL

About Sanquin Diagnostiek B.V.

Blood is of vital importance

We are the only organization in the world that can boast in-house medical, pharmaceutical and even scientific knowledge and expertise. With almost 3,000 committed colleagues and 350,000 blood donors, we can provide a better life for 300,000 patients each year.

Our Services (58)


ic

AH50 Assay

Price on request

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).

Show less

ic

Genotyping and Gene Expression Assays

Price on request

Bloodtype genotyping (including allelic discriminations, clotting diseases and red cell deficiencies)

Bloodtype genotyping (including allelic discriminations, clotting diseases and red cell deficiencies)

Show less
Hematology

ic

Leukemia Gene Panel

Price on request

Leukemia markers/ gene expression (eg, p190, bcr, braf, myd88 etc)

Leukemia markers/ gene expression (eg, p190, bcr, braf, myd88 etc)

Show less

ic

HLA Typing

Price on request

HLA typing (low- and high resolution using NGS)

HLA typing (low- and high resolution using NGS)

Show less

ic

RNA Extraction and Purification

Price on request

Nucleic extractions (DNA and RNA)

Nucleic extractions (DNA and RNA)

Show less

ic

DNA Extraction

Price on request

Nucleic extractions (DNA and RNA)

Nucleic extractions (DNA and RNA)

Show less

ic

Cell Proliferation Assays

Price on request

We can perform proliferation assays based on 3H thymidine incorporation and/or proliferation dye.

We can perform proliferation assays based on 3H thymidine incorporation and/or proliferation dye.

Show less

ic

Monocyte Activation Testing

Price on request
Request a quote for more information about this service.

ic

ELISpot Assays

Enzyme-Linked ImmunoSpot Assays
Price on request
Request a quote for more information about this service.

ic

Cytokine Analysis

Price on request
Request a quote for more information about this service.

ic

Natural Killer Cell Cytotoxicity Assay

Price on request

Description of the assay:
1. Effector cells (E):
a. positive (CD56+) selection of NK cells from fresh blood-isolated peripheral mononuclear blood cells (PBMC) using magnetic beads– preferred method of selection
b. negative (all other cells but NK cells depleted from PBMC)
2. Target cells (T):
a. SKBR3 breast carcinoma cell... Show more »

Description of the assay:
1. Effector cells (E):
a. positive (CD56+) selection of NK cells from fresh blood-isolated peripheral mononuclear blood cells (PBMC) using magnetic beads– preferred method of selection
b. negative (all other cells but NK cells depleted from PBMC)
2. Target cells (T):
a. SKBR3 breast carcinoma cell line, positive for Her2/Neu, possible to opsonize with trastuzumab to perform antibody-dependent cellular cytotoxicity assay (ADCC)
b. A431 colon carcinoma cell line, positive for EGFR, possible to opsonize with cetuximab to perform ADCC
c. Raji Burkitt lymphoma cell line, positive for CD20, possible to opsonize with rituximab to perfrom ADCC
3. Assay: target cells are labeled with radioactive 51Cr , and level of release is measured after incubation with NK cells, assay is performed in technical triplicate

General design of the assay:
- Target cell lines of interest tested (can test all three cell lines, depending on the final design either all target cell lines in parallel or separately)
- Test both spontaneous and ADCC (ADCC is about 2-fold higher than spontaneous cytotoxicity)
- Test 6 different healthy individuals
- Test different E:T ratio
- Step 1: test one concentration of all n=x compounds in these conditions
- Step 2: depending on the result, choose fixed E:T ratio and perform dose-dependency with n=x compounds

Additional assays/ expertise:
Besides the NK cell cytotoxicity assays, we have several assay set-ups available to determine activation, proliferation and differentiation of immune cells, mainly using flow cytometry as a read-out. We generally focus on complete peripheral blood mononuclear cells or isolated T cells for these type of analyses, but these can of course also be applied to NK cells.

Show less

ic

B Cell Activation Assay

Price on request
Request a quote for more information about this service.

ic

Mixed Lymphocyte Reactions

Price on request
Request a quote for more information about this service.

ic

Immunophenotyping

Price on request
Request a quote for more information about this service.

ic

Cell-Based Assays

Price on request
Request a quote for more information about this service.

ic

Clinical PK/PD Studies

Price on request
Request a quote for more information about this service.

ic

Bioanalysis

Price on request
Request a quote for more information about this service.

ic

Clinical Immunogenicity Testing

Price on request
Request a quote for more information about this service.

ic

Human Blood Sample Processing

Price on request
Request a quote for more information about this service.

ic

Biomarkers

Price on request

Biomarkers Services

Biomarkers Services

Show less

ic

Biology

Price on request

Biology Services

Biology Services

Show less

ic

Clinical Trials, Consulting, and Management

Price on request

Clinical Trials, Consulting, and Management Services

Clinical Trials, Consulting, and Management Services

Show less

ic

Pharmacology & Toxicology

Price on request

Pharmacology & Toxicology Services

Pharmacology & Toxicology Services

Show less

ic

Clinical Immunology Tests

Price on request

Clinical Immunology Tests Services

Clinical Immunology Tests Services

Show less

ic

Blood Banking and Transfusion Medicine

Price on request

Blood Banking and Transfusion Medicine Services

Blood Banking and Transfusion Medicine Services

Show less

ic

Clinical Hematology

Price on request

Clinical Hematology Services

Clinical Hematology Services

Show less

ic

Clinical Laboratory Services

Price on request

Clinical Laboratory Services

Clinical Laboratory Services

Show less

ic

Clinical Research

Price on request

Clinical Research Services

Clinical Research Services

Show less

ic

Immunoassays

Price on request

Immunoassays Services

Immunoassays Services

Show less

ic

Bioanalytical Assays

Price on request

Bioanalytical Assays Services

Bioanalytical Assays Services

Show less

ic

Epitope Mapping

Price on request

eg. Selection CD8+ T-cell epitopes, antigen-specific cytotoxic T-cell monitoring and On/ off target screening.

eg. Selection CD8+ T-cell epitopes, antigen-specific cytotoxic T-cell monitoring and On/ off target screening.

Show less

ic

Antibody Services

Price on request

Antibody Services

Antibody Services

Show less

ic

Cell Viability & Proliferation Assays

Price on request

Cell Viability & Proliferation Assays Services

Cell Viability & Proliferation Assays Services

Show less

ic

Testing of Cell Lines

Price on request

Testing of Cell Lines Services

Testing of Cell Lines Services

Show less

ic

Cells and Tissues

Price on request

Cells and Tissues Services

Cells and Tissues Services

Show less

ic

Protein Quantification

Price on request

Protein Quantification Services

Protein Quantification Services

Show less

ic

Protein Purification

Price on request

Protein Purification and Quantification Services

Protein Purification and Quantification Services

Show less

ic

Protein Services

Price on request

Protein Services

Protein Services

Show less

ic

Biochemistry & Molecular Biology

Price on request

Biochemistry & Molecular Biology Services

Biochemistry & Molecular Biology Services

Show less

ic

Immune Cell Assays

Price on request

Immune Cell Assays Services

Immune Cell Assays Services

Show less

ic

Functional & Cell Type Specific Assays

Price on request

Functional & Cell Type Specific Assays Services

Functional & Cell Type Specific Assays Services

Show less

ic

Gene Panel Testing

Price on request

Gene Panel Testing Services

Gene Panel Testing Services

Show less

ic

Clinical Molecular Diagnostics

Price on request

Clinical Molecular Diagnostics Services

Clinical Molecular Diagnostics Services

Show less

ic

RNA Services

Price on request

RNA Services

RNA Services

Show less

ic

DNA Extraction and Purification

Price on request

DNA Extraction and Purification Services

DNA Extraction and Purification Services

Show less

ic

DNA Services

Price on request

DNA Services

DNA Services

Show less

ic

Nucleic Acid Services

Price on request

Nucleic Acid Services

Nucleic Acid Services

Show less

ic

Blood Vessel Staining

Price on request

Mesenteric vessel staining

Isolate, fix and staining of vessels

Mesenteric vessel staining

Isolate, fix and staining of vessels

Show less

ic

Transwell Assay

Price on request

Migration- and Transmigration assays

Migration- and Transmigration assays

Show less

ic

CH50 Assay

Price on request

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).

Amongst our validated markers: C1INH (including inhibitor activity, Ag conc, Ab conc, Ab neutralising capacity), C1q, C3, C3d, C4, sC5b-9, C5, CH50, AP50, LP act, cytokines. In addition, we deliver the advantage to combine studies with our capailities in cellular assays (eg investigating Th/ Treg-cells responses).

Show less

ic

Cell Permeability Assay

Price on request

Cell Permeability Assay - leakage assay

Day 1
Seed epithelial cell line H292 in RPMI / 10%FCS / PS / L-glut
12WP transwell membrane
0.4 μm pore
12mm membrane
8.0•104 / well
RPMI / 10% FCS / 1% L-glutamin

Note/ It is not possible to view the forming monolayer through the membrane; therefore, seed a similar amount of... Show more »

Cell Permeability Assay - leakage assay

Day 1
Seed epithelial cell line H292 in RPMI / 10%FCS / PS / L-glut
12WP transwell membrane
0.4 μm pore
12mm membrane
8.0•104 / well
RPMI / 10% FCS / 1% L-glutamin

Note/ It is not possible to view the forming monolayer through the membrane; therefore, seed a similar amount of cells in a 24WP for comparison. The 12mm TW has a growth area of 1cm2; the wells from a 24WP have a growth area of 2cm2; seed two wells of a 24WP with 8.0•104 / well and 2 wells of a 24WP with 16•104 / well

Day 2 / 3
Refresh medium

Day 4
Remove phonol red:
Wash cells and bottom of TW with
Hepes containing 0.5% CeAlb
Transfer TW to new/clean 12WP

Add Dextran-Fluorescein 3000 and / or drugs
Allow dextrin-fluorescein to pass through membrane for 2 hrs
Measure λex 494 nm / λem 521 nm on an elisa plate reader
Magellan Fluorescein Standard: λex 485 nm / λem 535 nm

Show less

ic

Cell Invasion & Migration Assays

Price on request

Cell Invasion & Migration Assays Services

Cell Invasion & Migration Assays Services

Show less

ic

Drug Discovery & Development

Price on request

Drug Discovery & Development Services

Drug Discovery & Development Services

Show less

ic

Cell Death Assays

Price on request

Cell Death Assays Services

Cell Death Assays Services

Show less

ic

In vitro Immunogenicity Assays

Price on request
Request a quote for more information about this service.

ic

Pharmacology

Price on request

Pharmacology Services

Pharmacology Services

Show less

ic

ELISA

Enzyme-linked immunosorbent assay
Price on request
Request a quote for more information about this service.

ic

DNA Sequencing

Price on request
Request a quote for more information about this service.

Not finding what you're looking for?

Get info on this provider's capabilities without requesting a quote.
Missing Avatar

Perry Verzaal

Director Business Development

Sanquin Diagnostiek B.V. has not received any reviews.

Sanquin Diagnostiek B.V. has not received any endorsements.