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Samsara Sciences, Inc.

17 Orders Completed
San Diego, California, US

About Samsara Sciences, Inc.

Human biology is complex. Researchers today are challenged to conduct experiments that reveal the key pathways and mechanisms that drive function and disease at the cellular level.

There’s no choice more important than the living cells used to answer these questions.

Samsara Sciences is dedicated to... Show more »

Human biology is complex. Researchers today are challenged to conduct experiments that reveal the key pathways and mechanisms that drive function and disease at the cellular level.

There’s no choice more important than the living cells used to answer these questions.

Samsara Sciences is dedicated to the provision of high quality primary human liver and kidney cells for research applications. Our cellular products are supported by extensive data and the knowledge and expertise of our staff, scientific collaborators, and scientific advisors. From donor characteristics to the phenotypic attributes of isolated cells, each lot of cells is powered with the information you need to interpret your outcomes accurately.

Why choose Samsara?

  • Selection. Multiple lots available with diverse origins.
  • Quality. Traceable, viable, reliable.
  • Support. Culture and cryopreservation protocols provided with each lot.
  • Comprehensive. Access to a full spectrum of companion products, including formalin-fixed, paraffin-embedded tissues and snap-frozen tissues.

Choose wisely. Choose Samsara.

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Our Services (9)


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Human Kupffer Cells

Price on request

Kupffer Cells, the resident macrophages of the liver, are the largest population of tissue macrophages in the body. Distributed along the sinusoids of the liver, they play roles in both host defense and in tissue homeostasis. Phagocytosis and inducible cytokine response are key functional features of Kupffer... Show more »

Kupffer Cells, the resident macrophages of the liver, are the largest population of tissue macrophages in the body. Distributed along the sinusoids of the liver, they play roles in both host defense and in tissue homeostasis. Phagocytosis and inducible cytokine response are key functional features of Kupffer cells. Samsara’s Kupffer cells are isolated from liver tissue immediately after enzymatic dissociation and enriched prior to cryopreservation. Isolated hKC mount a cytokine response to pro-inflammatory stimuli such as lipopolysaccharide (LPS) and exhibit phagocytic activity, and the population is comprised of cells that are immunopositive for CD11b and CD68.

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Human Hepatic Stellate Cells

Price on request

Samsara Sciences offers primary cryopreserved adult hHSC from both healthy and diseased liver tissues for use in research applications. hHSC can be propagated using industry-standard protocols for stellate cells, and detailed culture protocols and media recipes are provided with each lot. Hepatic stellate cells... Show more »

Samsara Sciences offers primary cryopreserved adult hHSC from both healthy and diseased liver tissues for use in research applications. hHSC can be propagated using industry-standard protocols for stellate cells, and detailed culture protocols and media recipes are provided with each lot. Hepatic stellate cells have long been recognized as key players in liver homeostasis and pathogenesis, and activation of stellate cells secondary to fatty liver disease and drug
-induced liver injury is an accepted component of the progression of liver injury with fibrosis. Human stellate cells are identified by expression of glial fibrillary acidic protein (GFAP) and, to a lesser extent, desmin. Activated stellate cells gain expression of smooth muscle actin (SMA) and may also begin producing collagen and secreting TGFβ. Standard monolayer culture of HSC leads to culture activation of the cells, with a reduction in GFAP expression and increase in SMA expression over time. Samsara’s human HSC are available standardly as cryopreserved stock at passage 2, and earlier passage cells may be available upon request.

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Human Liver-Derived Endothelial Cells

Price on request

Samsara Sciences offers primary cryopreserved adult hLEC to support a broad array of research applications. hLEC can be propagated using industry-standard protocols for endothelial cells, and retain an endothelial cell morphology through limited serial passage. Phenotypic markers associated with liver-derived endothelial cells are... Show more »

Samsara Sciences offers primary cryopreserved adult hLEC to support a broad array of research applications. hLEC can be propagated using industry-standard protocols for endothelial cells, and retain an endothelial cell morphology through limited serial passage. Phenotypic markers associated with liver-derived endothelial cells are expressed by hLEC and can be assessed using standard flow cytometric analyses or immunocytochemical analyses. Isolated populations of hLEC generally comprise cells that are immunopositive for CD31, CD32b, vWF, LYVE-1, CD34, and CD299 (L-SIGN), though the specific expression patterns and proportions of cells positive for a particular marker may vary from lot to lot due to inherent differences in donor characteristics. Phenotypic shifts are also common with extended time in culture and/or serial passage, and can depend greatly on culture conditions.

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Human Primary Cells

Price on request

Human Hepatocytes (HC) from Samsara are isolated from viable human liver and cryopreserved immediately after purification. Isolated Hepatocytes are available in both plateable and suspension formats. Standard product characterization includes lot-specific post-thaw yield and viability. Comprehensive donor history report includes... Show more »

Human Hepatocytes (HC) from Samsara are isolated from viable human liver and cryopreserved immediately after purification. Isolated Hepatocytes are available in both plateable and suspension formats. Standard product characterization includes lot-specific post-thaw yield and viability. Comprehensive donor history report includes medical history and board-certified pathologist assessment of NAFLD activity score (NAS) and Batts-Ludwig fibrosis score. All livers come from consented donors and test negative for HIV I/II, HBV, HCV and Syphilis prior to acceptance. Healthy and diseased donors available.

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Human Non-alcoholic fatty liver disease (NAFLD) hepatocytes Primary Cells Biology Drug Discovery Show 6 more tags Show less

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RNA Sequencing

Price on request

RNA-Seq Data Library

Compilation of RNA sequencing performed on whole liver tissue and each individual liver cell type including Hepatooctyes, Stellates, Kupffer Cells and Endothelial Cells from DonorMatched Sets of 3 Healthy Donors and 5 confirmed NAFLD Donors. Sequencing includes both long non-coding RNA and mRNA. Raw data files plus DEG Seq2 data, GO analysis and FASTQ files.

RNA-Seq Data Library

Compilation of RNA sequencing performed on whole liver tissue and each individual liver cell type including Hepatooctyes, Stellates, Kupffer Cells and Endothelial Cells from DonorMatched Sets of 3 Healthy Donors and 5 confirmed NAFLD Donors. Sequencing includes both long non-coding RNA and mRNA. Raw data files plus DEG Seq2 data, GO analysis and FASTQ files.

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Human Fibrotic Disease Non-Alcoholic Steatohepatitis (NASH) Non-alcoholic fatty liver disease (NAFLD) RNA-Seq whole tissue hepatocytes stellates Kupffer Cells Endothelial Cells Drug Discovery Illumina HiSeq Show 12 more tags Show less

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Tissue Microarrays

Price on request

NAFLD Human Tissue Arrays from Samsara are prepared from formalin-fixed, paraffin-embedded (FFPE) tissue blocks. Arrays are comprised of 24 tissue cores from 6 independent donors with confirmed NAFLD scores ranging from 0-6 and no history of alcohol use. Tissues are mounted on positively charged slides and remain unstained... Show more »

NAFLD Human Tissue Arrays from Samsara are prepared from formalin-fixed, paraffin-embedded (FFPE) tissue blocks. Arrays are comprised of 24 tissue cores from 6 independent donors with confirmed NAFLD scores ranging from 0-6 and no history of alcohol use. Tissues are mounted on positively charged slides and remain unstained without cover. Available in packs of 2 or 5 slides with the option to assemble a custom block from our existing inventory. Tissue arrays are ideal for immunohistochemistry (IHC), histochemical stains, and in-situ hybridization (ISH). Comprehensive donor history report for each specimen includes relevant medical history and board-certified pathologist assessment of NAFLD activity score (NAS) and Batts-Ludwig fibrosis score. All livers come from consented donors and test negative for HIV I/II, HBV, HCV and Syphilis prior to acceptance.

CORE DIAMETER:   3 mm

NUMBER OF CORES:   24

NUMBER OF DONORS:   6

CORES PER DONOR:   4

DONOR AVAILABILITY:   Confirmed NAFLD scores ranging from 0-6

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Frozen Human Tissue

Price on request

Snap-Frozen human liver tissue

Samsara Sciences offers formalin-fixed, paraffin-embedded liver tissue samples as well as snap-frozen liver tissue samples, both of which are accompanied by a donor history report that includes histopathological scores for inflammation, fibrosis, and non-alcoholic steatohepatitis (NAS). ... Show more »

Snap-Frozen human liver tissue

Samsara Sciences offers formalin-fixed, paraffin-embedded liver tissue samples as well as snap-frozen liver tissue samples, both of which are accompanied by a donor history report that includes histopathological scores for inflammation, fibrosis, and non-alcoholic steatohepatitis (NAS). Samples available span NAFLD/NAS spectrum from 0-6. High quality FFPE-Blocks suitable for in situ hybridization and Immunohistochemical staining.

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Hepatology Non-Alcoholic Steatohepatitis (NASH) Non-alcoholic fatty liver disease (NAFLD) Liver Disease

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Fixed Human Tissue

Price on request

**Formalin Fixed Paraffin Embedded human liver tissue** Samsara Sciences provides FFPE-blocks of human liver tissue across a spectrum of NAFLD/NASH donors (scores 0-6) . Each block is approximately 5 x 3 x 2 mm and embedded in IHC-grade paraffin. A comprehensive Donor History Report including past medical history and... Show more »

**Formalin Fixed Paraffin Embedded human liver tissue** Samsara Sciences provides FFPE-blocks of human liver tissue across a spectrum of NAFLD/NASH donors (scores 0-6) . Each block is approximately 5 x 3 x 2 mm and embedded in IHC-grade paraffin. A comprehensive Donor History Report including past medical history and medications is included with each donor lot.

Unstained Formalin-Fixed Paraffing Embedded slides, also available.  Two tissue sections per slide.  

 

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Human Fibrotic Disease Non-Alcoholic Steatohepatitis (NASH) Non-alcoholic fatty liver disease (NAFLD) Liver Disease Primary Cells Biology Drug Discovery Toxicology Show 9 more tags Show less

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Human Renal Proximal Tubular Epithelial Cells

Price on request

The proximal tubule of the kidney is the primary site of exposure to a variety of toxicants, and thus is a key cell type for use in studies aimed at modeling or measuring drug responses. The proximal tubule also plays an active role in several homeostatic processes, including protein uptake, drug and solute transport, and... Show more »

The proximal tubule of the kidney is the primary site of exposure to a variety of toxicants, and thus is a key cell type for use in studies aimed at modeling or measuring drug responses. The proximal tubule also plays an active role in several homeostatic processes, including protein uptake, drug and solute transport, and metabolism. Isolated proximal tubular cells that possess and maintain phenotype and function over time may serve as useful tools in compound screening or as components of complex, multi-cellular model systems.

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  • Positive review for Tissue Preparation:

    January 2018

    "I had a positive experience with Samsara Sciences. They responded to all my communications within hours and the tissue was shipped as described with annotations. I would order from them again."

  • Positive review for Human Tissue Procurement:

    March 2017

    "The staff was friendly and communication, purchase and the shipment processes were largely satisfactory. Thanks!"

Samsara Sciences, Inc. has not received any endorsements.