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Samsara Sciences, Inc.

San Diego, CA, US

Human biology is complex. Researchers today are challenged to conduct experiments that reveal the key pathways and mechanisms that drive function and disease at the cellular level. There’s no choice more important than the living cells used to answer the questions.

Samsara Sciences is dedicated to the provision of high quality primary human liver and kidney cells for research applications. Our cellular products are supported by extensive data and the knowledge and expertise of our staff, scientific collaborators, and scientific advisors. From donor characteristics to the phenotypic attributes of isolated cells, each lot of cells is powered with the information you need to interpret your outcomes accurately.

Why choose Samsara?

  • Selection. Multiple lots available with diverse origins.
  • Quality. Traceable, viable, reliable.
  • Support. Culture and cryopreservation protocols provided with each lot.
    -... Show more »

Human biology is complex. Researchers today are challenged to conduct experiments that reveal the key pathways and mechanisms that drive function and disease at the cellular level. There’s no choice more important than the living cells used to answer the questions.

Samsara Sciences is dedicated to the provision of high quality primary human liver and kidney cells for research applications. Our cellular products are supported by extensive data and the knowledge and expertise of our staff, scientific collaborators, and scientific advisors. From donor characteristics to the phenotypic attributes of isolated cells, each lot of cells is powered with the information you need to interpret your outcomes accurately.

Why choose Samsara?

  • Selection. Multiple lots available with diverse origins.
  • Quality. Traceable, viable, reliable.
  • Support. Culture and cryopreservation protocols provided with each lot.
  • Comprehensive. Access to a full spectrum of companion products, including formalin-fixed, paraffin-embedded tissues and snap-frozen tissues.

Choose wisely. Choose Samsara.

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Samsara Sciences, Inc. has not listed any services.

Human Hepatic Sinusoidal Endothelial Cells
Price on request

Samsara offers human liver-derived endothelial cells for research use. These cells can be propagated over multiple passages with preservation of CD31, LYVE1, vWF, CD34, and CD299 expression levels. Expression of CD32b is observed by immunocytochemistry. Cells are isolated from primary human liver preparations by elutriating centrifugation.

Samsara offers human liver-derived endothelial cells for research use. These cells can be propagated over multiple passages with preservation of CD31, LYVE1, vWF, CD34, and CD299 expression levels. Expression of CD32b is observed by immunocytochemistry. Cells are isolated from primary human liver preparations by elutriating centrifugation.

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Human Renal Tubular Epithelial Cells
Price on request

Samsara Sciences offers primary human renal proximal tubular cells and companion growth media. Our cells express important transport proteins, including Na+K+ATPase, MDR1, OCT2, and OAT3, express megalin and cubilin and are able to uptake albumin, and maintain a stable epithelial morphology in vitro.

Samsara Sciences offers primary human renal proximal tubular cells and companion growth media. Our cells express important transport proteins, including Na+K+ATPase, MDR1, OCT2, and OAT3, express megalin and cubilin and are able to uptake albumin, and maintain a stable epithelial morphology in vitro.

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Tissue Preparation
Price on request

Samsara Sciences offers formalin-fixed, paraffin-embedded liver tissue samples as well as snap-frozen liver tissue samples, both of which are accompanied by a donor history report that includes histopathological scores for inflammation, fibrosis, and non-alcoholic steatohepatitis (NAS).

Samsara Sciences offers formalin-fixed, paraffin-embedded liver tissue samples as well as snap-frozen liver tissue samples, both of which are accompanied by a donor history report that includes histopathological scores for inflammation, fibrosis, and non-alcoholic steatohepatitis (NAS).

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Human Renal Proximal Tubular Epithelial Cells
Price on request

The proximal tubule of the kidney is the primary site of exposure to a variety of toxicants, and thus is a key cell type for use in studies aimed at modeling or measuring drug responses. The proximal tubule also plays an active role in several homeostatic processes, including protein uptake, drug and solute transport, and... Show more »

The proximal tubule of the kidney is the primary site of exposure to a variety of toxicants, and thus is a key cell type for use in studies aimed at modeling or measuring drug responses. The proximal tubule also plays an active role in several homeostatic processes, including protein uptake, drug and solute transport, and metabolism. Isolated proximal tubular cells that possess and maintain phenotype and function over time may serve as useful tools in compound screening or as components of complex, multi-cellular model systems.

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Human Liver-Derived Endothelial Cells
Price on request

Samsara Sciences offers primary cryopreserved adult hLEC to support a broad array of research applications. hLEC can be propagated using industry-standard protocols for endothelial cells, and retain an endothelial cell morphology through limited serial passage. Phenotypic markers associated with liver-derived endothelial cells are... Show more »

Samsara Sciences offers primary cryopreserved adult hLEC to support a broad array of research applications. hLEC can be propagated using industry-standard protocols for endothelial cells, and retain an endothelial cell morphology through limited serial passage. Phenotypic markers associated with liver-derived endothelial cells are expressed by hLEC and can be assessed using standard flow cytometric analyses or immunocytochemical analyses. Isolated populations of hLEC generally comprise cells that are immunopositive for CD31, CD32b, vWF, LYVE-1, CD34, and CD299 (L-SIGN), though the specific expression patterns and proportions of cells positive for a particular marker may vary from lot to lot due to inherent differences in donor characteristics. Phenotypic shifts are also common with extended time in culture and/or serial passage, and can depend greatly on culture conditions.

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Human Kupffer Cells
Price on request

Kupffer Cells, the resident macrophages of the liver, are the largest population of tissue macrophages in the body. Distributed along the sinusoids of the liver, they play roles in both host defense and in tissue homeostasis. Phagocytosis and inducible cytokine response are key functional features of Kupffer... Show more »

Kupffer Cells, the resident macrophages of the liver, are the largest population of tissue macrophages in the body. Distributed along the sinusoids of the liver, they play roles in both host defense and in tissue homeostasis. Phagocytosis and inducible cytokine response are key functional features of Kupffer cells. Samsara’s Kupffer cells are isolated from liver tissue immediately after enzymatic dissociation and enriched prior to cryopreservation. Isolated hKC mount a cytokine response to pro-inflammatory stimuli such as lipopolysaccharide (LPS) and exhibit phagocytic activity, and the population is comprised of cells that are immunopositive for CD11b and CD68.

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Human Primary Cell Isolation
Price on request

Samsara Sciences offers primary human liver and kidney cells, specializing in liver non-parenchymal cells (Kupffer, endothelial, and stellate cells) from healthy and NASH tissues, and primary renal proximal tubular cells (RPTEC), for research use. We also offer custom isolation services and formats for liver and kidney.

Samsara Sciences offers primary human liver and kidney cells, specializing in liver non-parenchymal cells (Kupffer, endothelial, and stellate cells) from healthy and NASH tissues, and primary renal proximal tubular cells (RPTEC), for research use. We also offer custom isolation services and formats for liver and kidney.

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Human Hepatic Stellate Cells
Price on request

Samsara Sciences offers primary cryopreserved adult hHSC from both healthy and diseased liver tissues for use in research applications. hHSC can be propagated using industry-standard protocols for stellate cells, and detailed culture protocols and media recipes are provided with each lot. Hepatic stellate cells... Show more »

Samsara Sciences offers primary cryopreserved adult hHSC from both healthy and diseased liver tissues for use in research applications. hHSC can be propagated using industry-standard protocols for stellate cells, and detailed culture protocols and media recipes are provided with each lot. Hepatic stellate cells have long been recognized as key players in liver homeostasis and pathogenesis, and activation of stellate cells secondary to fatty liver disease and drug
-induced liver injury is an accepted component of the progression of liver injury with fibrosis. Human stellate cells are identified by expression of glial fibrillary acidic protein (GFAP) and, to a lesser extent, desmin. Activated stellate cells gain expression of smooth muscle actin (SMA) and may also begin producing collagen and secreting TGFβ. Standard monolayer culture of HSC leads to culture activation of the cells, with a reduction in GFAP expression and increase in SMA expression over time. Samsara’s human HSC are available standardly as cryopreserved stock at passage 2, and earlier passage cells may be available upon request.

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March 6, 2017

Positive review received for Human Tissue Procurement:

"The staff was friendly and communication, purchase and the shipment processes were largely satisfactory. Thanks!"

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