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Recombinant Protein Production Core (Northwestern University)

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Evanston, Illinois, US

About Recombinant Protein Production Core (Northwestern University)

The Recombinant Protein Production Core (rPPC) provides quality controlled recombinant proteins for researchers within the Northwestern Community (WCAS, McCormick, Feinberg) and also serves academic and industry researchers outside of Northwestern University. rPPC operates based on the two service models: (1) a Training model where Northwestern researchers use specialized bioreactor systems and participate in hands-on-training activities and (2) a Production model where staff carry out expression (mg to gm scale) and purification of recombinant or synthetic biologics, including potential therapeutic proteins and peptides, among others. Currently, the main focus of rPPC is to be a user-facility; the facility has parallel bioreactor systems for multiplexed lab-scale cultivation of microbial, insect, and mammalian cells. rPPC also serves as a production facility, providing low-cost recombinant biologics for researchers at Northwestern University.

rPPC’s goal is to transform research at Northwestern University by addressing a variety of unmet needs that can often stall cutting-edge projects. As a centralized protein production center rPPC facilitates the expression of proteins for a broad spectrum of research activities ranging from structure/function studies to producing protein therapeutic candidates such as monoclonal antibodies for in vitro and in vivo pre-clinical studies. Scalable, high-throughput cultivation enables screening mutants for phenotype studies, proteomics, stem cell characterization, media screening, strain and process optimization for the production of biochemicals, e.g. biofuels. High-throughput cell-free protein production interfaces well with HTA to “augment and expand Northwestern University’s community of multidisciplinary researchers focusing on areas of biomedical research relevant to NIH.” Protein purification completes the facility enabling a one-stop shop for researchers to clone, overexpress, and purify relevant biologics.

All prices are at our External Academic rate. For corporate rates, please contact us.

Our Services (21)


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Plasmid DNA Preparation

Price on request

The DNA provided by customer will be expressed in competent E.coli cells. The scaled up E.Coli culture will be grown in shaker flask. DNA will be extracted and purified using plasmid mini - or maxi-prep kit depending on customer's request.


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Bacterial Protein Expression

Price on request

Recombinant protein expression involves transforming bacterial cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The cells are then lysed to extract the expressed protein for subsequent purification. Protein can be expressed in shaker flasks or on a 1L or 5L scale in bioreactors.

Q Plus Bioreactor B Plus Bioreactor LB Medium TB Medium 2xYT Medium IPTG Ampicillin Kanamycin sulfate Chloramphenicol Tetracycline BioPAT MFCS Sartorius Bio-Rad GenePulse Electroporator E. coli Show 14 more tags Show less

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Baculovirus/Insect Cell Protein Expression

Price on request

Insect cells are cotransfected with wild type baculovirus DNA and transfer vector plasmid DNA containing the foreign gene to be expressed to generate recombinant baculovirus carrying the DNA of interest. Price is per Liter of culture. We use a Bac-to-Bac system, from Invitrogen and Sf9 cells.

Insects Yamato Incubator Bio-Rad Thermal Cycler T100

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Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
Price on request

Recombinant protein expression involving transfecting cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The cells are then either lysed to extract the expressed protein for downstream processing, or growth medium is collected for secreted protein. Culture growth optimization is possible by using Q Plus Fermenter (1L, up to 3 in parallel).

  1. CHO cells
  2. HEK293

New Brunswick Scientific Incubator Shaker I2400 NanoDrop 8000 spectrophotometer Thermo Forma Reach-In CO2 Incubator 3950 AKTAxpress Bio-Rad Gel Doc EZ Imager ProteinSimple WES PEI Cellfectin UNICORN Show 9 more tags Show less

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Protein Purification Process Development

Price on request

This service includes the steps immediately following protein expression and immediately preceding protein purification. Downstream processing is used to recover active recombinant protein. It includes cell separation by centrifugation, cell membrane disruption and cell lysis by high pressure homogenizer.


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Protein Purification

Price on request

A spectrum of downstream processes are used to recover active recombinant protein: cell separation by centrufugation, cell lysis membrane disruption and cell lysis by high pressure homogenizer, protein separation and fusion tag cleavage. Price is per cell mass from 1L of culture.

  1. Cell lysis – high pressure homogenization
  • High speed centrifugation
  • Ultracentrifugation
  • Affinity tagged (His tag, GST, MBP) protein purification by HPLC
  • Buffer exchange and concentration of protein
  • Quantitation of purified protein using NanoDrop

Emulsi-Flex C5 Beckman Coulter Avanti Centrifuge J-20 AKTAxpress UNICORN ProteinSimple WES NanoDrop 8000 spectrophotometer Bio-Rad Gel Doc EZ Imager Show 7 more tags Show less

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Western Blot

Price on request

The service includes analytical immune detection of a protein in the sample or cell lysates provided by a customer. Using Capillary Electrophoresis, the fully automated ProteinSimple Wes Simple Western size assay system will greatly accelerate protein detection with high precision and quality. This technology is based on nanovolume size-based protein separation that can be used to quantify proteomic profiles of any experimental protein samples, or clinical specimens for both biomarker discovery and diagnostics. Instead of two days you spend just three hours to get accurately quantitated, and reproducible result of 24 samples.

ProteinSimple WES Compass

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Monoclonal Antibody (mAb) Production

Price on request

Generation of a hybridoma cell line (secreting antibody) starts with immunization of mice with an antigen of interest (immunogen), and proceeds with fusion of immune splenocytes and myeloma cells to make hybrid cells (hybridomas) that are to be screened to select the ones that produce a specific antibody. Standard screening assays used against the injected antigen (immunogen) include indirect ELISA and Western Blot. Currently rPPC offers only three steps of the hybridoma generation process – immunization, fusion and screening. After two rounds of screening, 96-well plates with growing young hybridoma cells will be transferred to the customer’s laboratory for hybridoma cell preservation by freezing down, and single cell cloning of the selected hybridoma cell line.

PEG1500 HAT Selection Medium HT Medium Fetal Bovine Serum Mouse Thermo Forma Reach-In CO2 Incubator 3950 ProteinSimple WES BioTek Synergy H1-M Microplate Reader BioTek microplate washer Show 9 more tags Show less

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Antibody Purification

Price on request

Hybridoma culture medium (1L), containing secreted antibody is purified through an automatic liquid chromatography system. The technique separates antibodies on the basis of a chromatography matrix. If a highly concentrated hybridoma supernatant (from our bioreactor) is to be purified, the above price applies to a 50 mL unit.

Beckman Coulter Avanti Centrifuge J-20 AKTAxpress Bio-Rad Profinia FPLC Protein G Protein A Protein A & G Pre-packed column GE UNICORN Show 8 more tags Show less

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Transfection

Price on request

A plasmid carrying the target DNA provided by customer will be inserted into an expression vector. Cells (bacterial, insect and mammalian) are transformed/transfected with an expression vector carrying the target DNA, encoding the protein of interest.

Insects Hamster Human Bio-Rad GenePulse Electroporator New Brunswick Scientific Incubator Shaker I2400 Yamato Incubator Ampicillin Kanamycin sulfate Chloramphenicol E. coli Show 10 more tags Show less

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Cell and Tissue Culture

Price on request
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Protein Production

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DNA Services

Price on request
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Biology

Price on request
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Protein Services

Price on request
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Protein Expression Visualization

Price on request
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Nucleic Acid Services

Price on request
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Cells and Tissues

Price on request
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Antibody Services

Price on request
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Biochemistry & Molecular Biology

Price on request
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Plasmids and Vectors

Price on request
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Izolda Popova

Assistant Director
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Jared Schooley

Research Technologist

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