Reaction Biology
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Malvern, Pennsylvania, US
Reaction Biology provides scientists worldwide with a trusted research partner supporting all phases of preclinical drug discovery from target validation through hit identification, hit to lead and lead optimization. Our portfolio covers most of your needs in drug discovery – over 1,000 biochemical targets, hundreds of cell-based assays, an extensive array of in vivo oncology models, along with custom assay development and protein production. Our team of Ph.D. scientists will provide you with collaborative support, quality data, and excellent communication, for a superior research result.
MST ASSAY
MST assays measure the interactions directly in solution without the need of immobilization to a surface (immobilization-free technology). MST uses a small amount of samples and allows for quick assay development and affinity determination (KD) of interactions. It can be used to detect binding between any kind of biomolecules including proteins, DNA, RNA, peptides, small molecules, fragments and ions, with quick measurement times and low sample consumption. The detection range of MST on Binding affinity is between pM to mM range.
Selection of suitable cell lines for in vivo efficacy studies.
The Hollow Fiber Assay is suited to investigate the impact of a drug on three cell lines simultaneously in one mouse.
Approach:
The fibers are loaded with a suspension of tumor cells and get closed on both ends. Fibers of three cell lines get implanted subcutaneously and/or intraperitoneally into one mouse. The mouse will than be treated with the test substance for several weeks. At the end of the study, the fibers get isolated from the mouse body and the number of living cells will be quantified.
This is a fast and economical approach to select suitable cell lines for in vivo studies to investigate the impact of a drug on the growth of tumors.
Please see more information on our website:
https://www.proqinase.com/products-services-vivo-testing-services/vivo-hollow-fiber-model
Xenograft and syngeneic tumor mouse models for efficacy testing of anti-cancer drugs.
Available models: subcutaneous, orthotopic and metastasizing models.
Studies with custom cell lines are possible.
All of our models are validated and up and running. Data for standard of care treatments are available.
Please see a list of all models on our website:
https://www.proqinase.com/products-service/vivo-testing-services
Xenograft and syngeneic tumor mouse models for efficacy testing of anti-cancer drugs.
Orthotopic models are available for colon, breast, pancreatic, skin, brain, kidney and prostate cancer.
Establishment of tumor models with custom cell lines is possible.
All of our models are validated and up and running. Data for standard of care treatments are available.
Please see a list of all models on our website:
https://www.proqinase.com/products-service-vivo-testing-services/orthotopic-tumor-models-xenograft-models-syngeneic-models
Metastasis tumor models are designed to analyze the anti-metastatic effects of anti-cancer drug candidates.
Syngeneic and xenograft models are available with intravenous, intracardial or orthotopic implantation.
Approach: Luciferase-transduced tumor cells with metastatic potential are implanted in mice. Metastasis is monitored via whole body imaging during the treatment period. After termination of the study, tumor cells will be quantified in a variety of organs such a lung, brain, liver or spleen via ex vivo luciferase analysis.
Bone marrow engraftment of leukemia cells will be performed after cyclophosphamide treatment.
Please see more information on our homepage:
https://www.proqinase.com/products-services-vivo-testing-services/metastasis-tumor-models-xenograft-models-syngeneic-models
Immuno-oncology mouse models
Syngeneic mouse models for efficacy testing of anti-cancer drugs with optional investigation of tumor-infiltrating immune cell populations.
Our immuno-oncology platform comprises a panel of syngeneic tumor models for testing of immune-modulating drugs. We offer models with subcutaneous and orthotopic implantation as well as metastasis models.
Our models are evaluated by treatment with anti-PD1, anti-PDL1 and anti-CTLA4 treatment regarding their inhibitory impact on tumor growth as well as their effects on tumor-infiltrating immune cells.
Flow cytometry will be used to characterize changes to the several immune cell subsets such as macrophages, myeloid derived suppressor cells, T cells, dendritic cells and neutrophils.
Syngeneic mouse models for efficacy testing of anti-cancer drugs.
Our immuno-oncology platform comprises a panel of syngeneic tumor models for testing of immune-modulating drugs. We offer models with subcutaneous and orthotopic implantation as well as metastasis models.
Our models are evaluated by treatment with anti-PD1, anti-PDL1 and anti-CTLA4 treatment regarding their inhibitory impact on tumor growth as well as their effects on tumor-infiltrating immune cells.
Flow cytometry will be used to characterize changes to the several immune cell subsets such as macrophages, myeloid derived suppressor cells, T cells, dendritic cells and neutrophils.
For more information please see our website:
https://www.proqinase.com/products-services-vivo-testing-services/immuno-oncology-platform
Flow Cytometry will be used to investigate expression levels of certain proteins on or in cells and to perform phenotyping of immune cell populations in mice.
Immune phenotyping in mice will be performed subsequent to an efficacy study of an immune-modulating drug in a syngeneic tumor mouse model.
We can investigate T lymphocytes, myeloid-derived suppressor cells, macrophages, dendritic cells, neutrophils in tumor tissue, lymphatic tissue or blood.
Please find more information and study examples on our homepage here:
https://www.proqinase.com/products-services-vivo-testing-services/immuno-oncology-platform
Our InVivo Angiogenesis Model is suited for assessing the pro- or anti-angiogenic effects of drug candidates by analysing their impact on vessel formation of human endothelial cells in mice.
Approach:
Spheroids from human endothelial cells (HUVECs) are implanted subcutaneously in mice in a matrix containing angiogenic growth factors. The formation of a human vasculature can be monitored in vivo and ex vivo and the effect of treatment can be determined.
Please see more information on our homepage:
https://www.proqinase.com/products-service-vivo-testing-services/vivo-angiogenesis-model
This service is suited to determine the maximum dose of a drug for a subsequent efficacy study.
Approach:
We use a staggered approach with 3 mice receiving one dose at the time. We can either do single treatment MTD studies or treat the mice for several weeks.
The MTD study can be performed with tumor-bearing or tumor-free mice.
Reaction Biology offers NQO1 for profiling services.
Reaction Biology offers cIAP-1, cIAP-2, XIAP for profiling services.
Our CL100-Prolifiler is a 2D proliferation assay suited for analysis of cytotoxic and anti-proliferative effects of test substances on a panel of 100 tumor cell lines.
Approach:
Tumor cells will be seeded in multiwell plates and incubated with the test substance for 3 days. After the incubation period, the amount of live cells will be quantified. This assay is usually performed for determination of IC50 values of the test substance.
Advantages:
Simple assay allowing for testing of large quantities of compounds in a rapid cost-efficient fashion.
We offer Free Choice Cell Proliferation Assays to choose a selection of tumor cell lines from our panel of 130 established cell lines.
On a quarterly basis we screen our entire panel of tumor cells lines. Any customer is welcome to provide one or several test items. Because all items will be tested in one run, this is an economic approach for a comprehensive overview of the efficacy of the test substance in a variety of tumor cells from different origins.
Please find all of our cell lines on our homepage:
https://www.proqinase.com/products-services-cellular-assay-services/cell-proliferation-assays
Reaction Biology offers Bcl-2 and BCL-xL for HTS and profiling services.
Synonymous for Soft Agar Assay or Clonogenic Assay
This assay is suited to investigate the impact of a drug on anchorage-independent growth of tumor cells.
Approach:
A single cell suspension will get mixed with soft agar and pipetted into a 96-well plate. The soft agar hardens making the cells float - not touching the plastic.
The drug will be applied to the wells and incubated for up to three weeks. The tumor cells proliferate and the single cells start forming colonies. At the end of the incubation period, the amount of cells per well will be quantified.
Please find more information on our homepage here:
https://www.proqinase.com/products-services-cellular-assay-services/soft-agar-assays-clonogenic-assays
Reaction Biology offers CYP 1A2, CYP 2C19, CYP 2C9, CYP 2D6, and CYP 3A4 for HTS and profiling.
Tumor spheroids are used to screen compounds in a near-physiological tumor situation.
Monospheroids are available for testing as well as co-culture spheroids assembled from tumor and stroma cells.
Approach:
Spheroids autonomously assemble in U-bottom 96-well plates. Incubation of compounds for three days with subsequent quantification of viable cells.
In co-culture spheroids the amount of stroma as well as tumor cells will be measured simultaneously via detection of renilla luciferase (stroma cells) and firefly luciferase (tumor cells).
High-throughput screening possible.
For more information please see our website
https://www.proqinase.com/products-service-cellular-assay-services/3d-tumor-spheroid-assays
Reaction Biology offers bromodomain HTS and profiling services. RBC also offers other reader domain proteins as assay targets, such as chromodomains, MBTs, multiple domains, PHDs, SRAs, and tudor domains. Assays available include AlphaScreen, Thermal Shift, and ITC. All bromodomain proteins are produced by Reaction Biology and are also available for purchase.
Oris assay to measure the anti-migratory effects of test substances on tumor cells.
Approach:
Tumor cells are seeded in a 96 well dish containing rubber stoppers. After cells reach confluency the stoppers will be removed, the test substance will be added to the medium and cells are allowed to migrate into the center of the dish. The amount of migrating cells will be determined by fluorescent labelling to determine the anti-migratory effect of the test substance.
Please find a list of all cell lines available for this assay as well as more information about the method on our homepage here:
https://www.proqinase.com/products-services-cellular-assay-services/cell-migration-assays
Assay for determination of anti- or pro-angiogenic effects of test substances on vessel formation by endothelial cells.
Approach:
Human endothelial cells (fibroblasts or smooth muscle cells may also be used) form spheroids by aggregation in a hanging drop system. The spheroids will be transferred into a gel-like matrix containing the test substance. The gel-like matrix allows the spheroids to float and vessels start sprouting into all directions. After 24 hours, the amount of newly formed vessels will be quantified to determine the anti- or pro-angiogenic effect of the test substance.
For more information about this assay, please see our homepage:
https://www.proqinase.com/products-service-cellular-assay-services/cellular-angiogenesis-assay
Generation of stable cell lines or primary cells via transfection methods (such as lipofection) or lenti-viral transduction methods.
Approach:
Primary or secondary cells get transduced or transfected. Successfully modified cells get selected and a polyclonal or monoclonal cell pool will be generated. The expression level of the protein of interest can be investigated via flow cytometry, Western blotting or luciferase activity assays.
Please find more information on our website
https://www.proqinase.com/products-services-cellular-assay-services/custom-tailored-cellular-assay-services
This assay is suited to investigate the impact of plasma on the activity of a kinase inhibitor.
Usually kinase inhibitors in cellular assay systems incubate in serum-free medium. When this drug is applied to a mouse or a human it will face high amounts of plasma, which potentially bind to the compound and decrease its activity.
Approach: We established two ways to investigate the activity of kinase inhibitors in the presence of plasma: incubation of the drug with plasma before investigating its kinase inhibitor activity in a cellular test system. The second possibility is to inject the drug into mice. Blood will be drawn which contains the drug which than will be tested for its kinase inhibitory activity in a cellular test system.
Both tests can be used in comparison with inhibitors that are known to be inhibited by plasma and are unaffected by surrounding plasma.
IC50 determination of the kinase inhibitory activity will be performed to determine the amount of freely-availalbe kinase inhibitor.
We have the experience and know-how to perform complete cancer drug discovery projects from hit finding, lead generation and optimization to proof of concept studies in mice. Lead optimization and medical chemistry will be done in cooperation with our renowned chemistry partners NovAliX, France, and Mercachem, The Netherlands.
Reaction Biology offers A20, Ataxin3, BAP1, NEDP1, SENP1, UCHL1, UCHL3, USP2, USP5, USP8, USP10, and USP14 for profiling services.
With our extensive experience of 20 years of kinase assay development and screening we are the right partner for the establishment of an assay for your kinase of interest.
We can either use a kinase that is commercially available or we can produce the kinase for you at our recombinant protein production site. Once we have the kinase in our hands, we will find a suitable substrate with our SubstrateFinder and examine the activity of the kinase with different substrate concentrations, ATP conditions and we might adjust co-factors or elements that increase the activity of the kinase if necessary.
Beside simple single-step assays we are also able to establish cascade-assays such as in the example available for download from our website:
https://www.proqinase.com/products-services-biochemical-assay-services/custom-tailored-biochemical-assay-services
Reaction Biology offers HAT profiling services for early drug discovery. Available targets are CBP, KAT5, MYST2, MYST4, p300, hGCN5 and pCAF.
Reaction Biology offers HSP90a and HSP90b for HTS and profiling.
The KinaseFinder is suited for the identification of kinases which phosphorylate a peptide or protein of interest.
Approach:
The customer provides a peptide or protein of his interest which will serve as a substrate. We will test which of our set of 339 kinases will phosphorylate this substrate. The KinaseFinder is ideal for the elucidation of physiological pathways.
Please find more information on our homepage
https://www.proqinase.com/products-services-biochemical-assay-services/kinasefinder
The Kinase SubstrateFinder is suited for the identification of a substrate for a certain kinase.
Approach:
Please find more information on our homepage
https://www.proqinase.com/products-services-biochemical-assay-services/kinase-substratefinder
ProQinase is globally known as kinase experts since 2001 for kinase screening and for production of high-quality kinase proteins with guaranteed activity. We provide off-the-shelf kinases and offer custom-tailored protein production to our customers.
Please see an overview of all kinase products on our website here:
https://www.proqinase.com/products-service/recombinant-proteins
We strive to produce full length kinases expressed in insect cells which are very near to the original kinase enzymes expressed in humans.
Importantly, all of our off-the-shelf active kinase enzymes are used once a month in our profiling services, which serves as perfect quality control to guarantee their activity. We can provide you information about suitable substrates and assay conditions which is an extremely valuable resource for our customers. Our scientists will also help you troubleshoot your kinase assays.
We routinely test the activity of our kinases in our radioactive assay format but also in the ATP consumption assay ADP-Glo.
Please find an overview of our custom-tailored protein production capacities on our homepage
https://www.proqinase.com/products-services-recombinant-proteins/custom-tailored-protein-production
Reaction Biology offers HDAC HTS and profiling services for early drug discovery. HDACs 1-11 are available as targets.
Reaction Biology offers IDH1 R132C, IDH1 R132H, IDH1 WT, IDH2 R140Q, IDH2 R172Q, and IDH2 WT for profiling services.
Please see above-mentioned description of "Preclinical Efficacy Testing" which can be performed with single drugs or a combination of drugs.
Reaction Biology offers radioisotope based HTS and profiling for 631 wild type, mutant, and atypical kinases. One free control compound is included in every assay and in your report.
Reaction Biology offers profiling for 17 lipid kinases. One free control compound is included in every assay and in your report.
Reaction Biology offers LSD1, as well as several other demethylase assays for profiling.
Please see above-mentioned "3D Tumor Model" or "Colony Formation Assay" (also known as Clonogenic Assay or SoftAgar Assay) for more information.
Drug Combination testing is available for in vivo efficacy studies (see "Preclinical Efficacy Studies") as well as in cellular testing systems such as the
"Cell Proliferation Assay"
"Compound Profiling in Cancer Cell Panels"
"3D Tumor Model"
"3D Cell Based Assay"
"Colony Formation Assay"
Reaction Biology offers the only 'Gold Standard' radioisotope based HMT assay service available for both profiling and low cost HTS. With over 30 HMTs available, RBC is able to offer the largest collection of HMTs for assay services and for purchase.
Please see above-mentioned description of "Kinase Assay Services".
Reaction Biology has several PARP assays available for HTS and profiling services, including PARP1, PARP2, and PARP5B.
Please see above-mentioned description of "Flow Cytometry" for more information.
Reaction Biology offers several phosphatase assays for HTS and profiling services. Targets include DUSP22_MKPX, PP1A, PP1B, PP2Aalpha, PTPN1_PTP1B, PTPN11_SHP2, PTPN12_PTP-PEST, PTPN2_TC-PTP, PTPN6-SHP1, PTPN7_LC-PTP, and PTPRC_CD45
Reaction Biology offers PDE HTS and profiling for early drug discovery. Targets include PDE10A, PDE1A, PDE2A, PDE3A, PDE4A, PDE4B, PDE4C, PDE4D, PDE5A, PDE7A, PDE8A, and PDE9A.
Please see the description of the "In vitro Angiogenesis Assay" above.
Reaction Biology offers more than 60 targets for protease profiling.
Please see above-mentioned description of "Cell Proliferation Assays".
Reaction Biology offers Sirtuin HTS and profiling services for early drug discovery. SIRT1, SIRT2, SIRT3, and SIRT5 are available as targets.
Please see above-mentioned desciption of "Preclinical Efficacy Studies".
Please see above-mentioned desciption of "Preclinical Efficacy Studies".
Thermal Analysis Services
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