Rapid Novor Inc

*** For a LIMITED TIME only, introductory discount to new customers in the first order.

You provide: Monoclonal antibody sample (>0.2mg, >95% purity, >0.5mg/mL concentration, without excessive BSA or other proteins)
We deliver: Full sequence of heavy and light chains; Full sequence coverage by LC-MS/MS; Comprehensive report.

Money-back guarantee – We guarantee at least 5X coverage of the CDRs, 3X coverage for 95% of the sequence, and 1X coverage for the full length. If the target coverage is not met, you will be fully refunded.

Service Overview

Rapid Novor offers antibody de novo sequencing service. We directly sequence the antibody protein using multiple enzyme digestion and high resolution LC-MS/MS. Our service guarantees full length sequencing of the heavy and light chains.

Our sequencing process does not require hybridoma, mRNA, or other sequence information. It provides a direct and independent insight into your antibody sample.

Accurate – We guarantee at least 5X coverage of CDRs in the variable regions. Every amino acid in the CDRs is confidently supported by fragment ions in at least five MS2 scans. For isomeric Leu and Ile, we strive to make correct inference from enzyme specificity and statistics in antibody database.

Comprehensive – As an option, we offer comprehensive characterization of the antibody protein. Our service includes the analyses of PTMs, glycosylation, disulphide bridges, contaminants, and other aspects customized to your needs.

Efficient – We use minimal amount of antibody sample (0.2 mg) and deliver results within 15 business days with consistent quality.

Method

Our team specializes in protein mass spectrometry and informatics. We develop proprietary workflow and software for antibody de novo sequencing. Our standard procedure consists of the following steps.

1. Digest the antibody protein into overlapping peptides with multiple enzymes.
   
2. Analyze the digestions with LC-MS/MS using a Thermo Orbitrap mass spectrometer.
   
3. Characterize overlapping peptides with de novo peptide sequencing.
   
4. Automated antibody sequence assembly from characterized peptides.
   
5. Result examination and annotation by our scientists.
   

*** For a LIMITED TIME only, introductory discount to new customers in the first order.

You provide: Monoclonal antibody sample (>0.2mg, >95% purity, >0.5mg/mL concentration, without excessive BSA or other proteins)
We deliver: Full sequence of heavy and light chains; Full sequence coverage by LC-MS/MS; Comprehensive report.

Money-back guarantee – We guarantee at least 5X coverage of the CDRs, 3X coverage for 95% of the sequence, and 1X coverage for the full length. If the target coverage is not met, you will be fully refunded.

Service Overview

Rapid Novor offers antibody de novo sequencing service. We directly sequence the antibody protein using multiple enzyme digestion and high resolution LC-MS/MS. Our service guarantees full length sequencing of the heavy and light chains.

Our sequencing process does not require hybridoma, mRNA, or other sequence information. It provides a direct and independent insight into your antibody sample.

Accurate – We guarantee at least 5X coverage of CDRs in the variable regions. Every amino acid in the CDRs is confidently supported by fragment ions in at least five MS2 scans. For isomeric Leu and Ile, we strive to make correct inference from enzyme specificity and statistics in antibody database.

Comprehensive – As an option, we offer comprehensive characterization of the antibody protein. Our service includes the analyses of PTMs, glycosylation, disulphide bridges, contaminants, and other aspects customized to your needs.

Efficient – We use minimal amount of antibody sample (0.2 mg) and deliver results within 15 business days with consistent quality.

Method

Our team specializes in protein mass spectrometry and informatics. We develop proprietary workflow and software for antibody de novo sequencing. Our standard procedure consists of the following steps.

1. Digest the antibody protein into overlapping peptides with multiple enzymes.
   
2. Analyze the digestions with LC-MS/MS using a Thermo Orbitrap mass spectrometer.
   
3. Characterize overlapping peptides with de novo peptide sequencing.
   
4. Automated antibody sequence assembly from characterized peptides.
   
5. Result examination and annotation by our scientists.
   

You provide: Monoclonal antibody sample (>0.2mg, >95% purity, >0.5mg/mL concentration, without excessive BSA or other proteins)
We deliver: 3 mg purified antibody in IgG format; QC data; Full sequence of heavy and light chains; Comprehensive sequencing report.

Money-back guarantee – We guarantee that the antibody we produced binds to the same antigen with similar affinity as the antibody sample provided.

Service Overview

Rapid Novor offers the "Sequencing + Expression" service to deliver antibodies that works. We directly sequence the antibody protein and then make a recombinant version of it. Our service guarantees that the antibody we produced binds to the same antigen with similar affinity as the antibody sample provided.

Method

The standard "Sequencing + Expression" service consists of the following steps:

1. Sequence the antibody protein using our proprietary antibody protein sequencing service.
   
2. Gene synthesis
   
3. Cloning into pUV vectors
   
4. Transient transfection of HEK293 cells
   
5. Affinity purification
   
6. Quality control to ensure purity (>98%), low endotoxin (<1EU/mg) and no albumin contamination
   

You provide: Monoclonal antibody sample (>0.2mg, >95% purity, >0.5mg/mL concentration, without excessive BSA or other proteins)
We deliver: Full sequence of heavy and light chains; Full sequence coverage by LC-MS/MS; Comprehensive report; PTM and Glycosylation report (optional) and Raw LC-MS/MS data.

Money-back guarantee – We guarantee at least 5X coverage of the CDRs, 3X coverage for 95% of the sequence, and 1X coverage for the full length. If the target coverage is not met, you will be fully refunded.

Promotion - We will sequence the first antibody protein for new customers at half of our regular price!

Service Overview

Rapid Novor offers antibody de novo sequencing service. We directly sequence the antibody protein using multiple enzyme digestion and high resolution LC-MS/MS. Our service guarantees full length sequencing of the heavy and light chains.

Our sequencing process does not require hybridoma, mRNA, or other sequence information. It provides a direct and independent insight into your antibody sample.

Accurate – We guarantee at least 5X coverage of CDRs in the variable regions. Every amino acid in the CDRs is confidently supported by fragment ions in at least five MS2 scans. For isomeric Leu and Ile, we strive to make correct inference from enzyme specificity and statistics in antibody database.

Comprehensive – As an option, we offer comprehensive characterization of the antibody protein. Our service includes the analyses of PTMs, glycosylation, disulphide bridges, contaminants, and other aspects customized to your needs.

Efficient – We use minimal amount of antibody sample (0.2 mg) and deliver results within 15 business days with consistent quality.

Method

Our team specializes in protein mass spectrometry and informatics. We develop proprietary workflow and software for antibody de novo sequencing. Our standard procedure consists of the following steps.

1. Digest the antibody protein into overlapping peptides with multiple enzymes.
   
2. Analyze the digestions with LC-MS/MS using a Thermo Orbitrap mass spectrometer.
   
3. Characterize overlapping peptides with de novo peptide sequencing.
   
4. Automated antibody sequence assembly from characterized peptides.
   
5. Result examination and annotation by our scientists.