Ranomics

Genetic variants close in promixity to exon-intron junctions have a high chance of perturbing gene function, which could result in disease causation.

To rapidly identify genetic variants that may disrupt splicing, Ranomics has developed a dual-fluorescence based reporter that is compatible with any exon-intron-exon junction of interest.

Our reporter system is compatible with a variety of readouts:

• microscopy
• fluorescence-activated cell sorting
• next generation sequencing

Ranomics' splicing reporter system can be adapted to characterize a single variant or thousands of variants in a single, streamlined workflow.

Our protein-protein interaction platform is ideal for high-resolution mapping of important surface residues on any protein.

Using Ranomics' VERG technology, Ranomics can measure binding interactions across thousands of protein variants in a single workflow. Binding events are detected in cells using fluorescence and measured using next-generation sequencing, providing maximum dynamic range and sensitivity.

Our partners can easily identify important surface residues for protein-protein interactions (including antibody-antigen interactions) and understand the mechanism of action for any protein-based drugs and biologics.

Ranomics' affinity maturation analysis platform is well suited for discovering better performing antibodies in a high-throughput and quantitative manner.

Starting with the parental antibody's native sequence, Ranomics' VariantFind library building technology produces diverse antibody libraries with high specificity and control.

Antibody libraries are transformed into and displayed on the surface of yeast cells or phages, followed by cycles of panning for antigen protein.

By conjugating soluble antigen proteins to fluorescence markers, Ranomics can rapidly isolate and identify high-affinity antibody-antigen interactions using fluorescence-activated cell sorting and next-generation sequencing.

The microbes development strategy guide is a custom-made instruction manual for engineering microbes that will synthesize your target molecule of interest. Starting with the target molecule in mind, our in-house software will:

• Identify pathways and enzymes required to synthesize your target molecule
  -Identify competing metabolic pathways
  -Suggest genetic manipulations that will improve theoretical yields
  -Balance co-factors in the synthetic bioproduction pathway

This solution provides researchers with novel designer pathways for your molecule of choice and also comes with the necessary DNA components to accelerate discovery and proof-of-concept studies.

This solution provides a cheaper, faster and efficient way to start any microbe engineering project.

Ranomics takes advantage of its VariantFind™ platform to help organizations optimize their antibodies. VariantFind™ is a series of multiplex PCRs that effectively mutates multiple amino acid positions simultaneously. Desired mutations are directly encoded by oligonucleotides, providing high control and specificity during the mutagenesis process.

Each DNA variant library is created with great precision and accuracy. The target gene will be mutated in a series of proprietary PCR processes. Libraries can be fine-tuned to carry a specified number of mutations and customized codon distributions.

Using Ranomics' VERG technology, Ranomics can then measure binding interactions across these thousands of protein variants in a single workflow. Binding events are detected in cells using fluorescence and measured using next-generation sequencing, providing maximum dynamic range and sensitivity.

The VariantFind platform is a series of multiplex PCRs that effectively mutates multiple amino acid positions simultaneously. Desired mutations are directly encoded by oligonucleotides, providing high control and specificity during the mutagenesis process.

Each DNA variant library is created with great precision and accuracy. The target gene will be mutated in a series of proprietary PCR processes. Libraries can be fine-tuned to carry a specified number of mutations and customized codon distributions.

All libraries are validated by sequencing to ensure high quality and specificity.

Prioritize and identify genetic variants as potential drug targets using our VERG platform.

Ranomics' streamlined enables the characterization of thousands of genetic variants at once using a diverse set of biological readouts including:

-cellular growth
-post-translational modifications

• synthetic reporters

Our analyses can be conducted across multiple cell lines to assess whether any genetic variant contributes to disease etiology and can serve as an effective therapeutic target.

Accelerate cell-based studies with Ranomics and identify high-quality drug targets faster.

Ranomics can make any phage display library you need using our proprietary multi-site saturation mutagenesis libraries. Get flexible scanning or combinatorial antibody libraries cloned into your phage display vector and we will screen them for you. Get results for your highest affinity antibodies in as fast as 8 weeks.

The VariantFind platform is a series of multiplex PCRs that effectively mutates multiple amino acid positions simultaneously. Desired mutations are directly encoded by oligonucleotides, providing high control and specificity during the mutagenesis process.

Each DNA variant library is created with great precision and accuracy. The target gene will be mutated in a series of proprietary PCR processes. Libraries can be fine-tuned to carry a specified number of mutations and customized codon distributions.

All libraries are validated by sequencing to ensure high quality and specificity.

Our protein-protein interaction platform is ideal for high-resolution mapping of important surface residues on any protein.

Using Ranomics' VERG technology, Ranomics can measure binding interactions across thousands of protein variants in a single workflow. Binding events are detected in cells using fluorescence and measured using next-generation sequencing, providing maximum dynamic range and sensitivity.

Our partners can easily identify important surface residues for protein-protein interactions (including antibody-antigen interactions) and understand the mechanism of action for any protein-based drugs and biologics.

The VariantFind platform is a series of multiplex PCRs that effectively mutates multiple amino acid positions simultaneously. Desired mutations are directly encoded by oligonucleotides, providing high control and specificity during the mutagenesis process.

Each DNA variant library is created with great precision and accuracy. The target gene will be mutated in a series of proprietary PCR processes. Libraries can be fine-tuned to carry a specified number of mutations and customized codon distributions.

All libraries are validated by sequencing to ensure high quality and specificity.

Our mutagenesis service consists of a series of multiplexed PCR reactions using targeted mutagenesis oligonucleotides that we designed using in-house software. The end product is a library of DNA constructs where each single DNA molecule will carry a single missense mutation

Our production process is flexible and scalable. There is no limit on target length and GC% composition. Additionally, we provide greater diversity compared to single-site saturation mutagenesis or targeted single site mutagenesis.

Ranomics provides its partners with a standalone gene mutagenesis service. Our one-of-a-kind process creates DNA variant libraries for any targeted region in your gene or an entire gene.

Our mutagenesis service consists of a series of multiplexed PCR reactions using targeted mutagenesis oligonucleotides that we designed using in-house software. The end product is a library of DNA constructs where each single DNA molecule will carry a single missense mutation

Our production process is flexible and scalable. There is no limit on target length and GC% composition. Additionally, we provide greater diversity compared to single-site saturation mutagenesis or targeted single site mutagenesis.

Multisite saturation mutagenesis (VariantFind) is a proprietary method developed by Ranomics to create comprehensive variant libraries. Each molecule in the variant library will carry variant(s) leading to a single missense mutation. Our mutagenesis service consists of a series of multiplexed PCR reactions using targeted mutagenesis oligonucleotides that we designed using in-house software. The end product is a library of DNA constructs where each single DNA molecule will carry a single missense mutation. We also provide a 96 well plate deliverable, where each well contains a single mutation.

VariantFind is well suited for scanning, single and combinatorial libraries.

Our production process is flexible and scalable. There is no limit on target length and GC% composition. Additionally, we provide greater diversity compared to single-site saturation mutagenesis or targeted single-site mutagenesis.

The VariantFind platform is a series of multiplex PCRs that effectively mutates multiple amino acid positions simultaneously. Desired mutations are directly encoded by oligonucleotides, providing high control and specificity during the mutagenesis process.

Each DNA variant library is created with great precision and accuracy. The target gene will be mutated in a series of proprietary PCR processes. Libraries can be fine-tuned to carry a specified number of mutations and customized codon distributions.

All libraries are validated by sequencing to ensure high quality and specificity.

The Ranomics knowledgebase is a cloud-based catalog of information on the functional impact of missense variants in cancer genes. See whether your variant of interest is functional or non-functional with our simple search interface, or upload a VCF to automatically append functional evidence to the variants in your list.

Genetic variants in bonafide cancer genes are a major concern for patients and health practitioners.

Harmful genetic variants can potentially increase an individuals' chance of developing cancer if the variants impact gene function. At Ranomics, we have developed a high-throughput synthetic lethality screening platform to test whether cancer gene (e.g. BRCA1 and BRCA2) variants impact gene function in cancer cell models.

Quickly understand whether a cancer gene variant is functionally important or pharmacologically actionable using our VERG platform.

The VariantFind platform is a series of multiplex PCRs that effectively mutates multiple amino acid positions simultaneously. Desired mutations are directly encoded by oligonucleotides, providing high control and specificity during the mutagenesis process.

Each DNA variant library is created with great precision and accuracy. The target gene will be mutated in a series of proprietary PCR processes. Libraries can be fine-tuned to carry a specified number of mutations and customized codon distributions.

All libraries are validated by sequencing to ensure high quality and specificity.

The VariantFind platform is a series of multiplex PCRs that effectively mutates multiple amino acid positions simultaneously. Desired mutations are directly encoded by oligonucleotides, providing high control and specificity during the mutagenesis process.

Each DNA variant library is created with great precision and accuracy. The target gene will be mutated in a series of proprietary PCR processes. Libraries can be fine-tuned to carry a specified number of mutations and customized codon distributions.

All libraries are validated by sequencing to ensure high quality and specificity.