R&D Systems

B-MoGen provided service to genetic engineer a one-for-one substitution of a DNA sequence in a genetic locus or an insertion of sequence not found within the locus. We utilizes targeted nuclease genome editing to insert desired sequences anywhere in the genome or change endogenous sequences, in your cells of interest. We strive to create the best models available to suit your research needs.

Gene Knockout

B-MoGen offers custom gene knockout services for Any Gene in Any Cell Line. Using proprietary methods and targeted Nucleases, we completely DELETE entire genes, exons, or desired regions of the genome. Request a quote for your gene in your cells today!

Gene Knock-In

B-MoGen provided service to genetic engineer a one-for-one substitution of a DNA sequence in a genetic locus or an insertion of sequence not found within the locus. We utilizes targeted nuclease genome editing to insert desired sequences anywhere in the genome or change endogenous sequences, in your cells of interest. We strive to create the best models available to suit your research needs.

Whether you need to deliver a reporter or a custom gene cassette our multiple Transposon systems are your answer. We'll generate a custom cell line with our proprietary transposon multi-gene cassette providing stable expression of up to SIX transgenes at once.

Custom Sleeping Beauty Transposon Vector

B-MoGen offers top of the line multi-gene expression vectors delivered via the proven Sleeping Beauty transposon system. Integrate and stably express up to SIX genes at once. Put your genes of interest into any cell.

Custom Gene Targeting Vector

Designing a targeted Knock-In experiment? Let us help. We'll design and build the optimal targeting vector for efficiently introducing your genes of interest into your cells.

Our scientists have developed methods to express and purify some of the most challenging proteins with the highest bioactivities on the market.

Delivery: We will produce exactly what you need and provide a test sample. If it meets your requirements, we will scale up production to meet your research and development needs. Available Services • Recombinant protein expression and purification (with typical high purity, high bioactivity, and low endotoxin levels) • In vitro protein modification and processing • Biotinylation • PEGylation • Fluorescent probe labeling • Incorporation of non-standard chemical labels • Point mutations • Animal Component-Free Process, Animal-Free™, & GMP-grade • Formulation to your requirements • Hundreds of unique, non-catalog items available

B-MoGen Biotechnologies is offering the worlds first Mitochonrial DNA Editing service. We have partnered our gene editing experience with the world's leading researchers in adapting targeted nucleases for use in mitochondria. Inquire with us to start your first Mitochondrial DNA Editing project.

REPRODUCIBLE, QUANTITATIVE ANALYSIS OF IDENTITY, PURITY AND HETEROGENEITY PROFILES FOR
YOUR THERAPEUTIC PROTEINS
✓ Method development
✓ Method transfers from other platforms
✓ Run samples for an established assay*

Capillary isoelectric focusing (cIEF) available

• Biotinylation and fluorescent probe labeling available 
• AviTag
• Alkaline Phophatase conjugation
• HRP conjugation
• PE conjugation
• APC conjugation
• PerCP conjugation
• Alexafluor conjuagtion

SINGLE-CELL PROTEIN EXPRESSION ANALYSIS
✓ Antibody screening
✓ Assay development
✓ scWest chip scanning
✓ Run samples for an established assay*

Generate in vitro transcribed mRNA CAR transcripts for target antibody.  scFV cloning with different versions and signaling domains and linkers.  Confirmation via binding to labeled ligand. 

Bio-Techne is utilizing its pioneering Cloudz™ dissolvable hydrogel to design a cell selection system that enables the gentle capture and release of select cell populations. Using principles for cell selection, this technology is ideal for simplifying cell manufacturing protocols while reducing the risk of bead contamination during downstream cell culture processing. This technology is currently available as a custom service.

Proliferation assay service available, as well as 900+ different bioassays established at R&D Systems brand.

Starting material is cDNA plasmids provided by customer or starting with R&D Systems cDNA.  Generate in vitro transcribed mRNA encoding target using R&D Systems vectors.  mRNA characterization done by concentration (NanoDrop), purity of mRNA by absorbance, and mRNA integrity by visualization on denaturing formaldehyde gel.  Deliverable is the mass of mRNA, shipped on dry ice.

Conduct qPCR testing in samples.  Deliverable will be a data report.  Samples run in duplicate with positive and negative controls.

Formulate custom medium and conduct QC testing to customer specifications.  Deliverables include data report and the bottles or provided container.  QC tests include endotoxin testing via LAL method, plus pH testing, osmolarity, and bioburden test.

We will run your samples via our Mycoplasma detection kit, Catalog # CUL001B.

To provide an accurate and highly sensitive tool for routine screening of mycoplasma contamination in cultured cells, R&D Systems developed the MycoProbe Mycoplasma Detection Assay. This assay detects Mycoplasma 16S ribosomal RNA (rRNA) using a colorimetric signal amplification system with sensitivity comparable to PCR. The MycoProbe assay is not susceptible to common problems encountered with PCR-based mycoplasma detection kits.

The MycoProbe Mycoplasma Detection Assay:

• Detects the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination.
• Is highly sensitive (comparable to PCR).
• Is compatible with high-throughput screening.
• Does not generate false positives from amplicon contamination.
• Can be used for cell culture supernatants or cultured cell pellets.
• Can be used with samples from fresh or frozen cells.
• Does not require cells to be cultured in antibiotic-free media.
• Includes a synthetic DNA oligonucleotide positive control.
• Generates results in 4.5 hours.

https://www.rndsystems.com/products/mycoprobe-mycoplasma-detection-kit_cul001b

Generate your species target construct in custom cloning vector backbone with flanking restriction sites and removal of internal restriction site.  Deliverable cDNA mass.

Our high quality cDNA is produced in a short amount of time allowing us to fulfill your request quickly. Economical pricing means you don’t have to sacrifice quality to meet your budget.

Provide us with a protein accession number and we can create your custom vector. All genes of interest are fully sequenced. We can provide the full length open reading frame or specific domains. We can also generate specific mutations such as point or frame-shifting mutations.

We offer multiple fluorescent and epitope tag versions of our expression vectors for easy analysis and visualization of protein expression levels. Tags can be added to the C- or N-terminus of the gene.

Available options:

• His
• Myc
• HA
• Flag/DDK
• hFc
• mFc

Available options:

• GFP

We will insert your customized gene into our shuttle vector, one of our expression vectors, or a specific vector that you provide.

Promoters available:

• CMV
• TK

Selection markers available:

• Puromycin
• Neomycin

We can tailor the cDNA preparation process to meet your requirements. cDNA concentration, amount, buffer, and lyophilization can all be adjusted to meet your specifications.

Range of bioanalytical services with turnaround time from 3-10 business days.  SEC, DLS, HPLC, MS, DSF, cIEF, Gly-Q, N-terminal sequencing, SEC-SLS, MFI, SPR, and all conventional biochemical methods as well (SDS-PAGE, UV-visible spectrophotometry, custom designed experiments).

When specificity, long-term consistency, and supply are critical, trust the experts.

History: R&D Systems manufactures more than 12,500 primary antibodies, against over 3,600 target analytes, across 19 different species.

Expertise: Using in-house immunogens and proprietary technologies, R&D Systems generates the antibodies that define the industry leading Quantikine® ELISA kits.

Delivery: We tailor immunogen and immunization protocols to generate antibodies with high specificity and outstanding performance in your desired application. If your provided test sample meets your requirements, we will scale up production to meet your research and development needs.

Antibody Services

• Polyclonal and monoclonal antibody development
• Rabbit recombinant monoclonal antibody development
• Hybridoma to recombinant antibody conversion
• Polyclonal to recombinant antibody conversion
• Large-scale production, affinity-based purification
• Conjugation to fluorophores, HRP, and biotin
• Access to our hybridoma panels containing thousands of non-catalog sister clones
• Monoclonal production of client-supplied hybridomas

Immortalized Antibody Supply.

For applications requiring large amounts of consistent antibody, R&D Systems hybridoma and B-cell to recombinant antibody conversion service produces a dependable supply of custom engineered monoclonal antibody. R&D Systems proprietary processes guarantee yields that provide the gram quantities required for the development of diagnostic and therapeutic reagents.

Why R&D Systems Recombinant Antibody Conversion?

• R&D Systems antibodies support the industry-leading Quantikine® ELISAs
• Ability to scale up to gram quantity production
• A cost-effective and time-saving solution for superior antibodies
• The sequence and cell line(s) can be held by a third party to guarantee supply
• Consistent quality and performance over time

Available Engineering Services

• Modification of the species, isotype, hinge, and Fc region
• Generation of active truncated forms
• Improved yield without any change in affinity

The Process

1. Customer provides:
   a) Cell line expressing antibody or full-length antibody sequence.
   b) Antigen for testing the recombinant antibody using direct ELISA.
   c) Small aliquot of purified antibody.

2. Conversion, small scale expression of recombinant antibody, and comparison to the original hybridoma-derived antibody by R&D Systems.

3. Provision of data report and sample for customer's evaluation.

4. Exclusive supply of purified antibody.

5. Sequence and cell line(s) placed with third party for storage and security.

Panels of Non-Catalog Antibodies.

For every monoclonal antibody in our catalog, we have a panel of non-catalog antibodies available.
You can test these antibody panels in your application to find the antibody that produces optimal results.

Screen Antibody Panels to Identify Antibody Matched Pairs

The antibody matched pair you are looking for could be hiding in our selection of antibody panels.
Maximize your chances of successful antibody pairing by ordering our available antibody panels.

Consistent Antibody Supply

• Each antibody panel is produced in-house at R&D Systems, so we can meet your long-term supply needs.
• No 3rd party suppliers, which means no additional supply/licensing complications.
• All antibody panels are positive in direct ELISA for the analyte of interest.
• Convert any of our monoclonal antibodies to recombinant antibodies, and immortalize your antibody supply.
• Explore other antibody projects: antibody engineering, conjugation services, and more.

When specificity, long-term consistency, and supply are critical, trust the experts.

History: R&D Systems manufactures more than 12,500 primary antibodies, against over 3,600 target analytes, across 19 different species.

Expertise: Using in-house immunogens and proprietary technologies, R&D Systems generates the antibodies that define the industry leading Quantikine® ELISA kits.

Delivery: We tailor immunogen and immunization protocols to generate antibodies with high specificity and outstanding performance in your desired application. If your provided test sample meets your requirements, we will scale up production to meet your research and development needs.

Antibody Services

• Polyclonal and monoclonal antibody development
• Rabbit recombinant monoclonal antibody development
• Hybridoma to recombinant antibody conversion
• Polyclonal to recombinant antibody conversion
• Large-scale production, affinity-based purification
• Conjugation to fluorophores, HRP, and biotin
• Access to our hybridoma panels containing thousands of non-catalog sister clones
• Monoclonal production of client-supplied hybridomas

Accelerate biomarker discovery, validation, and detection with unique analyte panels.

History

With the largest immediately available retail analyte selection and an active development pipeline of new targets, R&D Systems has developed a reputation for delivering high quality and customized content for Luminex® assays.

Expertise

Building on the legacy of immunoassay development, our scientists can often translate Quantikine® ELISA reagents to the Luminex® platform and can optimize assay performance using decades of immunoassay experience.

Delivery

Utilizing our massive in-house collection of antibodies and proteins reduces development time, saves you money, and puts custom multiplex panels that detect your specific analytes of interest in your hands faster.

Available Services

• Unique analyte development
• Optimized panel configurations
• Sample type validation
• Evaluation of externally sourced antibodies
• Panel assembly to minimize required sample volume

Harness the unique experience and expertise of the R&D Systems Quantikine® ELISA team.

History: R&D Systems unrivaled reputation for quality has made Quantikine® ELISA Kits the gold standard and most referenced, ready-to-use ELISAs in the industry.

Expertise: Our scientists have developed hundreds of unique diluents and have decades of experience optimizing conditions to detect a range of analytes.

Delivery: Throughout the iterative immunoassay development process, our expert scientists and project management team will provide data reports and recommendations for assay improvements. As projects evolve, we have quality management systems and regulatory affairs in place to support every need, from basic discovery research, preclinical and clinical research through companion diagnostic applications.

Available Services

• Antibody matched pair screening
• Assay development and optimization
• Complete ELISA kit manufacturing
• Precision automated microplate coating
• Translation to automated platforms
• Facilitate the transition from RUO to IVD

Our R&D Systems scientists have developed methods to express and purify some of the most challenging proteins with the highest bioactivities on the market.

We will produce exactly what you need and provide a test sample. If it meets your requirements, we will scale up production to meet your research and development needs.

• Recombinant protein expression and purification (with typical high purity, high bioactivity, and low endotoxin levels)
• In vitro protein modification and processing
  • Biotinylation
  • PEGylation
  • Fluorescent probe labeling
  • Incorporation of non-standard chemical labels
• Point mutations
• Animal Component-Free Process, Animal-Free™, & GMP-grade
• Formulation to your requirements
• Hundreds of unique, non-catalog items available

Take advantage of over 900 established bioassays to measure bioactivity.

History: With more than 4,000 bioactive proteins developed and tested, and over 3,000 small molecules available to modulate responses, no other company matches the bioassay experience of Bio-Techne.

Expertise: We have more than 900 established bioassays available to test proteins, small molecules, and antibodies. Our scientists in Bioassay Services will consult with you to select and optimize an assay to deliver relevant results.

Delivery: Raw data are provided, along with a professionally formatted report and a detailed summary of the bioassay protocol employed.

Bioassays Include, but are not limited to

• Proliferation Assays
• Cytotoxicity/Survival/Apoptosis
• Cellular Differentiation
• Reporter Assays
• Cytokine Induction
• Chemotaxis
• Cell Adhesion
• Signal Transduction
• Neuronal Assays
• Ligand/Receptor Binding

Numerous techniques, including SPR, western, immunoassay, flow cytometry, IHC, and many more.  Let us know what detection based testing you may be searching for and we'll happily discuss feasibility, pricing, and share a relevant SOW if applicable.

• Polyclonal and monoclonal antibody development
• Rabbit recombinant monoclonal antibody development
• Hybridoma to recombinant antibody conversion
• Polyclonal to recombinant antibody conversion
• Large-scale production, affinity-based purification
• Conjugation to fluorophores, HRP, and biotin
• Access to our hybridoma panels containing thousands of non-catalog sister clones
• Monoclonal production of client-supplied hybridomas

A280 (Molecular Devices SPECTAmax PLUS microplate spectrophotometer), and also tests available for individual protein quantification including but not limited to ELISA, western, and mass spec, and Bradford protein assay, HPLC,

R&D Systems ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

Assays for the detection and enumeration of cytokine secreting cells

Assays for the detection and enumeration of cells secreting either of two cytokines

Fluorescence-based assays for the detection and quantitation of cells secreting either of two cytokines

R&D Systems brand develops and manufactures the gold standard Quantikine ELISA immunoassay kits.  As the manufacturer, our assays have been validated at the time of development so your sample testing in the BTS Lab can begin immediately—no extra validation fees or waiting weeks or months for an assay evaluation to be completed.  Our testing services range from a few plates to hundreds or thousands of plates of samples, and we have the bandwidth to complete your project timely.

Our Biomarker Testing Service laboratory operates under the R&D Systems corporate Quality Management System, which is ISO 9001:2008 and ISO 13485:2003 certified. Additional quality assurance is provided by an independent QA group that evaluates equipment, personnel, standard operating procedures, and study compliance.

Both gel and gel-free western blot services available.  We use our Simple Western, ProteinSimple brand analyzers for analysis.

Recombinant proteins are useful tools in understanding protein-protein interactions, such as receptor-ligand binding and extracellular matrix interactions. Therapeutic strategies often focus on these interactions. A common binding assay, known as a functional ELISA, involves the attachment of a recombinant receptor extracellular domain to a microplate, followed by the addition and detection of the ligand. Carrier-free proteins should be used when coating the microplate to avoid competition of the recombinant protein and carrier protein during the plate coating process.

Additionally, we have SPR (PPI, protein-protein interaction) services available.  Please inquire.

We have validated many reagents by immunoprecipitation.  Regarding our experience, here are a few immunoprecipitation conditions from our website.  Please contact us regarding your specific immunoprecipitation project!

Solid, cost effective library of pre-made cell lysates to validate your antibodies and eliminate the guesswork

Intracellular and surface staining by flow cytometry.  FACS Calibur and FACSCantoII, plus additionally Single Cell Western technique (Milo platform) when there aren't yet any good flow cytometry antibodies immediately available for intracellular targets (histones, transcription factors, etc).  R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, PerCP-, Alexa Fluor® 350-, 405-, 488-, 594-, 647-, 700-, 750-, or allophycocyanin-conjugated monoclonal antibodies specifically designed to monitor protein expression by flow cytometry, and we can conjugate antibodies on-demand, and assemble a panel of antibodies to detect and define cell populations.

When it comes to fast biomarker development, we’ve got you covered — from discovery with Luminex assays, to verification with Simple Western assays, and validation with Simple Plex assays.  Please inquire on the specific disease of interest and details about your specific project, sample type, biomarkers, and any related information.  Looking forward to your reply.

In addition to our off-the-shelf range of Tocriscreen compound libraries, we offer a custom compound library service. Our customer service team and scientifically trained personnel will be pleased to help you create a unique compound library, designed to meet your exact screening requirements.

• Select compounds from our list of available compounds
• Specify your desired quantities
• Choose between dry/solid format or in DMSO
• Stipulate the tube, vial, or plate to complement your compound handling system

We have significant experience in formulating proteins for optimal stability and shelf-life, while maintaining biological activity.  Please write us a note on your specific project details and we'll discuss feasibility and if applicable a Statement of Work will be generated.  We have a range of buffer formulations already and can assess the protein of interest and apply it to one of these buffer systems, and QC test.

Range of antibody modifications including antibody engineering, antibody deglycosylation, reformatting isotype, species switch, hinge region and Fc region edits.  Please inquire!

1.   From scratch, de novo monoclonal and polyclonal antibody development using multiple host species options including goat, mouse, rat, rabbit, and more

2.   Conversion of hybridoma to recombinant antibody

3.   Custom recombinant antibody engineering services including Fab’2, scFv, inserting mutations, bi-specific antibodies, and truncated active forms.

4.   Generate in vitro transcribed Chimeric Antigen Receptor (CAR) mRNA transcripts using scFv cloning

5.   GMP-grade antibody, documentation, and cell line testing

6.   Production of antibody under serum-free conditions

7.   Immunogen design tailored to meet your project needs, spanning peptide immunizations to full-length protein

8.   Endotoxin purification to meet your project specifications

9.   Epitope mapping

10.  Antibody deglycosylation

11.  Reformatting antibody including isotype, species switch, hinge region, and Fc region

12.  Large scale production with affinity-based purification to Gram quantities

13.  Conjugation to fluorophores, HRP, and biotin

14.  Application testing services including Western Blot, Immunohistochemistry and Immunocytochemistry, Flow Cytometry, ELISA

15.  Molecular weight confirmation by SDS-PAGE, SEC analysis, endotoxin levels, direct ELISA

16.  Developing antibodies validated for our ProteinSimple platforms

17.  Multiple product formats available including liquid and lypophilized

18.  Purification choices with standard Protein A/G resins and antigen- specific affinity

19.  For almost every R&D Systems clone that we have on our website, we often have an additional panel of clones to the same target

We are very successful in developing proteins, expression strategies, and purification; thousands of proteins both online and non-catalog.  Pilot expression can include CHO, NS0, HEK293, E.coli, depending on your protein of interest, and then we move foward with one cell line once we identify the right clone for transfection, selection, screening, and banking.    Range of QC tests include but not limited to concentration, purity, endotoxin, N-terminal sequencing, microbiology, bioassay. 

Range of polyclonal antibody production, including but not limited to goat and rabbit.  We will assist with antigen design, immunication, antibody purification, QC testing, cross-reacitivity testing, and formulation options.

Custom bottling and lyophilization services in a range of vial and bottling formats. 

Antibody purification via antigen affinity chromatography and range of techniques including Protein G.  Please inquire.

We have over 900 cell-based bioassays, please contact us to discuss your next project. 

In addition, if looking for signaling events, our Proteome Profiler™ Antibody Arrays allow for the measurement of up to 119 proteins in a single sample. These arrays require no specialized equipment and eliminate the need for multiple Western blot experiments. Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies. The arrays utilize chemiluminescence for detection and membranes can be assessed for protein levels in the same manner as traditional Western blots. Select arrays are also suitable for use with the LI-COR® and FluorChem™ detection systems.

R&D Systems ELISA's are the most trusted, most published ELISA's on the market with a menu of 700 target analytes spanning 12 species.  We have a range of immunoassay options spanning unique model organisms to murine to human species. 

Our master calibrated Quantikine® assays ensure that our kits perform consistently over time. Results obtained today are comparable to results obtained last month or even last year.

R&D Systems brand generates full-length, bioactive proteins.  In addition, if your project requires, we can develop smaller domains of proteins if you may need to generate antibodies directed towards a specific epitope recognition sequence.  Message us to discuss your next antigen development project, we may already have something in our own freezers to save you time and money.  We will assess with you feasibility if it may be a custom, from scratch recombinant antigen development project. 

Biacore T200 SPR sample testing.  Please inquire for price per sample testing.  Lead time from sample receipt to data report is typically less than 10 days. 

Cell and Tissue Culture Services

Targeted Protein Degradation (TPD) refers to the use of heterobifunctional small molecule Degraders (e.g. PROTACs™) to achieve knockdown of
a protein of interest. These small molecules are composed of an E3 ligase ligand and a target protein ligand, joined by a linker. Binding of both
ligands results in the formation of a ternary complex between E3 ligase and target protein, resulting in polyubiquitination of the protein and its
subsequent degradation by the proteasome.
Tocris provides a selection of commercially available building blocks to support Degrader research and development including functionalized
E3 ligase ligands and E3 ligase ligands conjugated to common linker groups. Tocris also offers a custom service for the design and synthesis
of Degrader building blocks for your targeted protein degradation research. Additionally, we can partner with your discovery project for the
custom design and synthesis of active Degraders.