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R&D Systems

6 Orders Completed
Minneapolis, Minnesota, US

About R&D Systems

Divison of: Bio-Techne

Founded: 1976  Type: Public Company  Size: 2000+ employees

We offer custom reagent and sample testing services spanning the R&D Systems brand product... Show more »

Founded: 1976  Type: Public Company  Size: 2000+ employees

We offer custom reagent and sample testing services spanning the R&D Systems brand product lines.

  • Recombinant protein development
  • Monoclonal and polyclonal antibody development
  • Hybridoma to recombinant antibody conversion and engineering
  • ELISA development and optimization
  • Luminex assay development 450+ analytes, customizable
  • Bioactivity testing, over 900 bioassays available
  • Biomarker testing services.  700+ targets spanning 12 species.  Sample testing spans discovery, pre-clinical, and clinical services across range of sample types and platforms (single analyte and multiplex).

When your work demands unique reagents or scientific support, turn to the decades of product development and manufacturing legacy at R&D Systems, a Bio-Techne brand.  We have over 2,100 employees, over 500,000 products, and 350,000+ journal citations.  We may already have what you need in our non-catalog, so send us a note and we can check on feasibility.

Since 1976, R&D Systems has been a developer and manufacturer of high-quality reagents for the scientific industry. As the world leader in research immunoassay development, our gold-standard Quantikine® ELISAs are built using recombinant proteins and panels of antibodies that are developed and manufactured in-house. Our products are produced in North America, under a stringent Quality Management System in our ISO-certified, FDA-regulated facility. Together with a dedicated project manager, our expert scientists, quality assurance team, and world-class technical support we will deliver solutions exactly tailored to bring you success faster and more economically.

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Our Services (42)


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Recombinant Protein Expression

Price on request

Our scientists have developed methods to express and purify some of the most challenging proteins with the highest bioactivities on the market.

Delivery: We will produce exactly what you need and provide a test sample. If it meets your requirements, we will scale up production to meet your research... Show more »

Our scientists have developed methods to express and purify some of the most challenging proteins with the highest bioactivities on the market.

Delivery: We will produce exactly what you need and provide a test sample. If it meets your requirements, we will scale up production to meet your research and development needs. Available Services • Recombinant protein expression and purification (with typical high purity, high bioactivity, and low endotoxin levels) • In vitro protein modification and processing • Biotinylation • PEGylation • Fluorescent probe labeling • Incorporation of non-standard chemical labels • Point mutations • Animal Component-Free Process, Animal-Free™, & GMP-grade • Formulation to your requirements • Hundreds of unique, non-catalog items available

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CAR-T Cell Therapy Development

Price on request

Generate in vitro transcribed mRNA CAR transcripts for target antibody.  scFV cloning with different versions and signaling domains and linkers.  Confirmation via binding to labeled ligand. 

Generate in vitro transcribed mRNA CAR transcripts for target antibody.  scFV cloning with different versions and signaling domains and linkers.  Confirmation via binding to labeled ligand. 

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T Cell Activation and Proliferation Assays

Price on request

Bio-Techne is utilizing its pioneering Cloudz dissolvable hydrogel to design a cell selection system that enables the gentle capture and release of select cell populations. Using principles for cell selection, this technology is ideal for simplifying cell manufacturing protocols while reducing the risk of bead... Show more »

Bio-Techne is utilizing its pioneering Cloudz dissolvable hydrogel to design a cell selection system that enables the gentle capture and release of select cell populations. Using principles for cell selection, this technology is ideal for simplifying cell manufacturing protocols while reducing the risk of bead contamination during downstream cell culture processing. This technology is currently available as a custom service.

Proliferation assay service available, as well as 900+ different bioassays established at R&D Systems brand.

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mRNA Synthesis

Price on request

Starting material is cDNA plasmids provided by customer or starting with R&D Systems cDNA.  Generate in vitro transcribed mRNA encoding target using R&D Systems vectors.  mRNA characterization done by concentration (NanoDrop), purity of mRNA by absorbance, and mRNA integrity by visualization on denaturing... Show more »

Starting material is cDNA plasmids provided by customer or starting with R&D Systems cDNA.  Generate in vitro transcribed mRNA encoding target using R&D Systems vectors.  mRNA characterization done by concentration (NanoDrop), purity of mRNA by absorbance, and mRNA integrity by visualization on denaturing formaldehyde gel.  Deliverable is the mass of mRNA, shipped on dry ice.

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qPCR

Quantitative PCR
Price on request

Conduct qPCR testing in samples.  Deliverable will be a data report.  Samples run in duplicate with positive and negative controls.

Conduct qPCR testing in samples.  Deliverable will be a data report.  Samples run in duplicate with positive and negative controls.

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qPCR

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Chemically Defined Cell Culture Media

Price on request

Formulate custom medium and conduct QC testing to customer specifications.  Deliverables include data report and the bottles or provided container.  QC tests include endotoxin testing via LAL method, plus pH testing, osmolarity, and bioburden test.

Formulate custom medium and conduct QC testing to customer specifications.  Deliverables include data report and the bottles or provided container.  QC tests include endotoxin testing via LAL method, plus pH testing, osmolarity, and bioburden test.

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Cell culture

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Mycoplasma Testing

Price on request

We will run your samples via our Mycoplasma detection kit, Catalog # CUL001B.

To provide an accurate and highly sensitive tool for routine screening of mycoplasma contamination in cultured cells, R&D Systems developed the MycoProbe Mycoplasma Detection Assay. This assay detects Mycoplasma 16S ribosomal RNA (rRNA)... Show more »

We will run your samples via our Mycoplasma detection kit, Catalog # CUL001B.

To provide an accurate and highly sensitive tool for routine screening of mycoplasma contamination in cultured cells, R&D Systems developed the MycoProbe Mycoplasma Detection Assay. This assay detects Mycoplasma 16S ribosomal RNA (rRNA) using a colorimetric signal amplification system with sensitivity comparable to PCR. The MycoProbe assay is not susceptible to common problems encountered with PCR-based mycoplasma detection kits.

The MycoProbe Mycoplasma Detection Assay:

  • Detects the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination.
  • Is highly sensitive (comparable to PCR).
  • Is compatible with high-throughput screening.
  • Does not generate false positives from amplicon contamination.
  • Can be used for cell culture supernatants or cultured cell pellets.
  • Can be used with samples from fresh or frozen cells.
  • Does not require cells to be cultured in antibiotic-free media.
  • Includes a synthetic DNA oligonucleotide positive control.
  • Generates results in 4.5 hours.

https://www.rndsystems.com/products/mycoprobe-mycoplasma-detection-kit_cul001b

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Mycoplasma Detection

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cDNA Cloning

Price on request

Generate your species target construct in custom cloning vector backbone with flanking restriction sites and removal of internal restriction site.  Deliverable cDNA mass.

Rapid Turnaround Time

Our high quality cDNA is produced in a short amount of time allowing us to fulfill your request quickly. Economical... Show more »

Generate your species target construct in custom cloning vector backbone with flanking restriction sites and removal of internal restriction site.  Deliverable cDNA mass.

Rapid Turnaround Time

Our high quality cDNA is produced in a short amount of time allowing us to fulfill your request quickly. Economical pricing means you don’t have to sacrifice quality to meet your budget.

Gene Isolation

Provide us with a protein accession number and we can create your custom vector. All genes of interest are fully sequenced. We can provide the full length open reading frame or specific domains. We can also generate specific mutations such as point or frame-shifting mutations.

Tagging Options

We offer multiple fluorescent and epitope tag versions of our expression vectors for easy analysis and visualization of protein expression levels. Tags can be added to the C- or N-terminus of the gene.

Epitope tags allow for easy protein purification and detection.

Available options:

  • His
  • Myc
  • HA
  • Flag/DDK
  • hFc
  • mFc

Fluorescent tags allow for easy protein tracking and visualization.

Available options:

  • GFP

Vector Type

We will insert your customized gene into our shuttle vector, one of our expression vectors, or a specific vector that you provide.

Promoters available:

  • CMV
  • TK

Selection markers available:

  • Puromycin
  • Neomycin

Formulation

We can tailor the cDNA preparation process to meet your requirements. cDNA concentration, amount, buffer, and lyophilization can all be adjusted to meet your specifications.

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Bioanalytical Assays

Price on request

Range of bioanalytical services with turnaround time from 3-10 business days.  SEC, DLS, HPLC, MS, DSF, cIEF, Gly-Q, N-terminal sequencing, SEC-SLS, MFI, SPR, and all conventional biochemical methods as well (SDS-PAGE, UV-visible spectrophotometry, custom designed experiments).

Range of bioanalytical services with turnaround time from 3-10 business days.  SEC, DLS, HPLC, MS, DSF, cIEF, Gly-Q, N-terminal sequencing, SEC-SLS, MFI, SPR, and all conventional biochemical methods as well (SDS-PAGE, UV-visible spectrophotometry, custom designed experiments).

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Monoclonal Antibody (mAb) Production

Price on request

When specificity, long-term consistency, and supply are critical, trust the experts.

History: R&D Systems manufactures more than 12,500 primary antibodies, against over 3,600 target analytes, across 19 different species.

Expertise: Using in-house immunogens and proprietary technologies, R&D Systems generates the antibodies... Show more »

When specificity, long-term consistency, and supply are critical, trust the experts.

History: R&D Systems manufactures more than 12,500 primary antibodies, against over 3,600 target analytes, across 19 different species.

Expertise: Using in-house immunogens and proprietary technologies, R&D Systems generates the antibodies that define the industry leading Quantikine® ELISA kits.

Delivery: We tailor immunogen and immunization protocols to generate antibodies with high specificity and outstanding performance in your desired application. If your provided test sample meets your requirements, we will scale up production to meet your research and development needs.

Antibody Services

• Polyclonal and monoclonal antibody development
• Rabbit recombinant monoclonal antibody development
• Hybridoma to recombinant antibody conversion
• Polyclonal to recombinant antibody conversion
• Large-scale production, affinity-based purification
• Conjugation to fluorophores, HRP, and biotin
• Access to our hybridoma panels containing thousands of non-catalog sister clones
• Monoclonal production of client-supplied hybridomas

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Recombinant Antibody Production

Price on request

Immortalized Antibody Supply.

For applications requiring large amounts of consistent antibody, R&D Systems hybridoma and B-cell to recombinant antibody conversion service produces a dependable supply of custom engineered monoclonal antibody. R&D Systems proprietary processes guarantee yields that provide the gram quantities... Show more »

Immortalized Antibody Supply.

For applications requiring large amounts of consistent antibody, R&D Systems hybridoma and B-cell to recombinant antibody conversion service produces a dependable supply of custom engineered monoclonal antibody. R&D Systems proprietary processes guarantee yields that provide the gram quantities required for the development of diagnostic and therapeutic reagents.

Why R&D Systems Recombinant Antibody Conversion?

• R&D Systems antibodies support the industry-leading Quantikine® ELISAs
• Ability to scale up to gram quantity production
• A cost-effective and time-saving solution for superior antibodies
• The sequence and cell line(s) can be held by a third party to guarantee supply
• Consistent quality and performance over time

Available Engineering Services

• Modification of the species, isotype, hinge, and Fc region
• Generation of active truncated forms
• Improved yield without any change in affinity

The Process

  1. Customer provides:
    a) Cell line expressing antibody or full-length antibody sequence.
    b) Antigen for testing the recombinant antibody using direct ELISA.
    c) Small aliquot of purified antibody.

  2. Conversion, small scale expression of recombinant antibody, and comparison to the original hybridoma-derived antibody by R&D Systems.

  3. Provision of data report and sample for customer's evaluation.

  4. Exclusive supply of purified antibody.

  5. Sequence and cell line(s) placed with third party for storage and security.

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Antibody Screening

Price on request

Panels of Non-Catalog Antibodies.

For every monoclonal antibody in our catalog, we have a panel of non-catalog antibodies available.
You can test these antibody panels in your application to find the antibody that produces optimal results.

Screen Antibody Panels to Identify Antibody Matched Pairs

The antibody matched... Show more »

Panels of Non-Catalog Antibodies.

For every monoclonal antibody in our catalog, we have a panel of non-catalog antibodies available.
You can test these antibody panels in your application to find the antibody that produces optimal results.

Screen Antibody Panels to Identify Antibody Matched Pairs

The antibody matched pair you are looking for could be hiding in our selection of antibody panels.
Maximize your chances of successful antibody pairing by ordering our available antibody panels.

Consistent Antibody Supply

• Each antibody panel is produced in-house at R&D Systems, so we can meet your long-term supply needs.
• No 3rd party suppliers, which means no additional supply/licensing complications.
• All antibody panels are positive in direct ELISA for the analyte of interest.
• Convert any of our monoclonal antibodies to recombinant antibodies, and immortalize your antibody supply.
• Explore other antibody projects: antibody engineering, conjugation services, and more.

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Polyclonal Antibody Development

Price on request

When specificity, long-term consistency, and supply are critical, trust the experts.

History: R&D Systems manufactures more than 12,500 primary antibodies, against over 3,600 target analytes, across 19 different species.

Expertise: Using in-house immunogens and proprietary technologies, R&D Systems generates the antibodies... Show more »

When specificity, long-term consistency, and supply are critical, trust the experts.

History: R&D Systems manufactures more than 12,500 primary antibodies, against over 3,600 target analytes, across 19 different species.

Expertise: Using in-house immunogens and proprietary technologies, R&D Systems generates the antibodies that define the industry leading Quantikine® ELISA kits.

Delivery: We tailor immunogen and immunization protocols to generate antibodies with high specificity and outstanding performance in your desired application. If your provided test sample meets your requirements, we will scale up production to meet your research and development needs.

Antibody Services

• Polyclonal and monoclonal antibody development
• Rabbit recombinant monoclonal antibody development
• Hybridoma to recombinant antibody conversion
• Polyclonal to recombinant antibody conversion
• Large-scale production, affinity-based purification
• Conjugation to fluorophores, HRP, and biotin
• Access to our hybridoma panels containing thousands of non-catalog sister clones
• Monoclonal production of client-supplied hybridomas

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Luminex Multiplex Assays

Price on request

Accelerate biomarker discovery, validation, and detection with unique analyte panels.

History

With the largest immediately available retail analyte selection and an active development pipeline of new targets, R&D Systems has developed a reputation for delivering high quality and customized content for Luminex®... Show more »

Accelerate biomarker discovery, validation, and detection with unique analyte panels.

History

With the largest immediately available retail analyte selection and an active development pipeline of new targets, R&D Systems has developed a reputation for delivering high quality and customized content for Luminex® assays.

Expertise

Building on the legacy of immunoassay development, our scientists can often translate Quantikine® ELISA reagents to the Luminex® platform and can optimize assay performance using decades of immunoassay experience.

Delivery

Utilizing our massive in-house collection of antibodies and proteins reduces development time, saves you money, and puts custom multiplex panels that detect your specific analytes of interest in your hands faster.

Available Services

• Unique analyte development
• Optimized panel configurations
• Sample type validation
• Evaluation of externally sourced antibodies
• Panel assembly to minimize required sample volume

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ELISA Kits

Price on request

Harness the unique experience and expertise of the R&D Systems Quantikine® ELISA team.

History: R&D Systems unrivaled reputation for quality has made Quantikine® ELISA Kits the gold standard and most referenced, ready-to-use ELISAs in the industry.

Expertise: Our scientists have developed hundreds of unique diluents and... Show more »

Harness the unique experience and expertise of the R&D Systems Quantikine® ELISA team.

History: R&D Systems unrivaled reputation for quality has made Quantikine® ELISA Kits the gold standard and most referenced, ready-to-use ELISAs in the industry.

Expertise: Our scientists have developed hundreds of unique diluents and have decades of experience optimizing conditions to detect a range of analytes.

Delivery: Throughout the iterative immunoassay development process, our expert scientists and project management team will provide data reports and recommendations for assay improvements. As projects evolve, we have quality management systems and regulatory affairs in place to support every need, from basic discovery research, preclinical and clinical research through companion diagnostic applications.

Available Services

• Antibody matched pair screening
• Assay development and optimization
• Complete ELISA kit manufacturing
• Precision automated microplate coating
• Translation to automated platforms
• Facilitate the transition from RUO to IVD

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Product Testing Services

Price on request

Take advantage of over 900 established bioassays to measure bioactivity.

History: With more than 4,000 bioactive proteins developed and tested, and over 3,000 small molecules available to modulate responses, no other company matches the bioassay experience of Bio-Techne.

Expertise: We have more than 900 established bioassays... Show more »

Take advantage of over 900 established bioassays to measure bioactivity.

History: With more than 4,000 bioactive proteins developed and tested, and over 3,000 small molecules available to modulate responses, no other company matches the bioassay experience of Bio-Techne.

Expertise: We have more than 900 established bioassays available to test proteins, small molecules, and antibodies. Our scientists in Bioassay Services will consult with you to select and optimize an assay to deliver relevant results.

Delivery: Raw data are provided, along with a professionally formatted report and a detailed summary of the bioassay protocol employed.

Bioassays Include, but are not limited to

• Proliferation Assays
• Cytotoxicity/Survival/Apoptosis
• Cellular Differentiation
• Reporter Assays
• Cytokine Induction
• Chemotaxis
• Cell Adhesion
• Signal Transduction
• Neuronal Assays
• Ligand/Receptor Binding

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Protein Production

Price on request

Expertise

Our R&D Systems scientists have developed methods to express and purify some of the most challenging proteins with the highest bioactivities on the market.

Delivery

We will produce exactly what you need and provide a test sample. If it meets your requirements, we will scale up production... Show more »

Expertise

Our R&D Systems scientists have developed methods to express and purify some of the most challenging proteins with the highest bioactivities on the market.

Delivery

We will produce exactly what you need and provide a test sample. If it meets your requirements, we will scale up production to meet your research and development needs.

  • Recombinant protein expression and purification (with typical high purity, high bioactivity, and low endotoxin levels)
  • In vitro protein modification and processing
    • Biotinylation
    • PEGylation
    • Fluorescent probe labeling
    • Incorporation of non-standard chemical labels
  • Point mutations
  • Animal Component-Free Process, Animal-Free™, & GMP-grade
  • Formulation to your requirements
  • Hundreds of unique, non-catalog items available
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Antibody/Antigen Detection Based Testing

Price on request

Numerous techniques, including SPR, western, immunoassay, flow cytometry, IHC, and many more.  Let us know what detection based testing you may be searching for and we'll happily discuss feasibility, pricing, and share a relevant SOW if applicable.

Numerous techniques, including SPR, western, immunoassay, flow cytometry, IHC, and many more.  Let us know what detection based testing you may be searching for and we'll happily discuss feasibility, pricing, and share a relevant SOW if applicable.

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Antibody Services

Price on request

Available Services

  • Polyclonal and monoclonal antibody development
  • Rabbit recombinant monoclonal antibody development
  • Hybridoma to recombinant antibody conversion
  • Polyclonal to recombinant antibody conversion
  • Large-scale production, affinity-based... Show more »

Available Services

  • Polyclonal and monoclonal antibody development
  • Rabbit recombinant monoclonal antibody development
  • Hybridoma to recombinant antibody conversion
  • Polyclonal to recombinant antibody conversion
  • Large-scale production, affinity-based purification
  • Conjugation to fluorophores, HRP, and biotin
  • Access to our hybridoma panels containing thousands of non-catalog sister clones
  • Monoclonal production of client-supplied hybridomas
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antibody conjugation Polyclonal Antibody Production Monoclonal Antibody Hybridoma to recombinant antibody Recombinant antibody Antibody engineering bispecific antibody antibody conversion Fluorochrome conjugation biotin conjugation HRP conjugation Show 11 more tags Show less

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Protein Quantification

Price on request

A280 (Molecular Devices SPECTAmax PLUS microplate spectrophotometer), and also tests available for individual protein quantification including but not limited to ELISA, western, and mass spec, and Bradford protein assay, HPLC,

A280 (Molecular Devices SPECTAmax PLUS microplate spectrophotometer), and also tests available for individual protein quantification including but not limited to ELISA, western, and mass spec, and Bradford protein assay, HPLC,

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HPLC Coomassie SpectraMax

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Enzyme-Linked Immunosorbent Spot

Price on request

R&D Systems ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot... Show more »

R&D Systems ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

Single Analyte ELISpot Kits

Assays for the detection and enumeration of cytokine secreting cells

Dual-Color ELISpot Kits

Assays for the detection and enumeration of cells secreting either of two cytokines

Dual-Color FluoroSpot Kits

Fluorescence-based assays for the detection and quantitation of cells secreting either of two cytokines

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ELISA

Enzyme-linked immunosorbent assay
Price on request

R&D Systems brand develops and manufactures the gold standard Quantikine ELISA immunoassay kits.  As the manufacturer, our assays have been validated at the time of development so your sample testing in the BTS Lab can begin immediately—no extra validation fees or waiting weeks or months for an assay evaluation... Show more »

R&D Systems brand develops and manufactures the gold standard Quantikine ELISA immunoassay kits.  As the manufacturer, our assays have been validated at the time of development so your sample testing in the BTS Lab can begin immediately—no extra validation fees or waiting weeks or months for an assay evaluation to be completed.  Our testing services range from a few plates to hundreds or thousands of plates of samples, and we have the bandwidth to complete your project timely.

Our Biomarker Testing Service laboratory operates under the R&D Systems corporate Quality Management System, which is ISO 9001:2008 and ISO 13485:2003 certified. Additional quality assurance is provided by an independent QA group that evaluates equipment, personnel, standard operating procedures, and study compliance.

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Fluorescence Activated Cell Sorting (FACS)

Fluorescence Activated Cell Sorting
Price on request
Request a quote for more information about this service.

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Western Blot

Price on request

Both gel and gel-free western blot services available.  We use our Simple Western, ProteinSimple brand analyzers for analysis.

Both gel and gel-free western blot services available.  We use our Simple Western, ProteinSimple brand analyzers for analysis.

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Protein-Protein Interaction Analysis

Price on request

Recombinant proteins are useful tools in understanding protein-protein interactions, such as receptor-ligand binding and extracellular matrix interactions. Therapeutic strategies often focus on these interactions. A common binding assay, known as a functional ELISA, involves the attachment of a recombinant receptor... Show more »

Recombinant proteins are useful tools in understanding protein-protein interactions, such as receptor-ligand binding and extracellular matrix interactions. Therapeutic strategies often focus on these interactions. A common binding assay, known as a functional ELISA, involves the attachment of a recombinant receptor extracellular domain to a microplate, followed by the addition and detection of the ligand. Carrier-free proteins should be used when coating the microplate to avoid competition of the recombinant protein and carrier protein during the plate coating process.

Additionally, we have SPR (PPI, protein-protein interaction) services available.  Please inquire.

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Immunoprecipitation

Price on request

We have validated many reagents by immunoprecipitation.  Regarding our experience, here are a few immunoprecipitation conditions from our website.  Please contact us regarding your specific immunoprecipitation project!

 

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We have validated many reagents by immunoprecipitation.  Regarding our experience, here are a few immunoprecipitation conditions from our website.  Please contact us regarding your specific immunoprecipitation project!

 

 

Wash Buffer

50 mM Tris, 150 mM NaCl, 2 mM EDTA, 1% NP-40, pH 7.4.

For immunoprecipitation of phosphorylation-dependent epitopes, phosphatase inhibitors may be added to the wash buffer. We recommend adding one or more of: 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3VO4, 10 mM NaF, or 1-2 mM EGTA.

Whole Cell Lysis Buffer

50 mM Tris, 150 mM NaCl, 2 mM EDTA, 1% NP-40,  1 mM sodium orthovanadate, 0.25 mM phenylmethylsulfonyl fluoride (PMSF), 1 µg/mL aprotinin, 1 µg/mL leupeptin, 1 µg/mL chymostatin, pH 7.4

Nuclear Extract Lysis Buffer

10 mM HEPES, 1.50 mM MgCl2, 10 mM KCl, 0.1% NP-40,  1 mM sodium orthovanadate, 0.25 mM phenylmethylsulfonyl fluoride (PMSF), 1 µg/mL aprotinin, 1 µg/mL leupeptin, 1 µg/mL chymostatin, pH 7.9

SDS Sample Buffer

0.25 M Tris, 6% SDS, 10% glycerol, 20 mM dithiothreitol, bromophenyl blue, pH 6.8
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Flow Cytometry

Price on request

Intracellular and surface staining by flow cytometry.  FACS Calibur and FACSCantoII, plus additionally Single Cell Western technique (Milo platform) when there aren't yet any good flow cytometry antibodies immediately available for intracellular targets (histones, transcription factors, etc).  R&D Systems offers a... Show more »

Intracellular and surface staining by flow cytometry.  FACS Calibur and FACSCantoII, plus additionally Single Cell Western technique (Milo platform) when there aren't yet any good flow cytometry antibodies immediately available for intracellular targets (histones, transcription factors, etc).  R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, PerCP-, Alexa Fluor® 350-, 405-, 488-, 594-, 647-, 700-, 750-, or allophycocyanin-conjugated monoclonal antibodies specifically designed to monitor protein expression by flow cytometry, and we can conjugate antibodies on-demand, and assemble a panel of antibodies to detect and define cell populations.

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FACS Calibur FACSCanto

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Biomarkers

Price on request

When it comes to fast biomarker development, we’ve got you covered — from discovery with Luminex assays, to verification with Simple Western assays, and validation with Simple Plex assays.  Please inquire on the specific disease of interest and details about your specific project, sample type, biomarkers, and any related information.  Looking forward to your reply.

When it comes to fast biomarker development, we’ve got you covered — from discovery with Luminex assays, to verification with Simple Western assays, and validation with Simple Plex assays.  Please inquire on the specific disease of interest and details about your specific project, sample type, biomarkers, and any related information.  Looking forward to your reply.

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Automated ELISA Luminex® Western IHC

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Compound Library Design

Price on request

In addition to our off-the-shelf range of Tocriscreen compound libraries, we offer a custom compound library service. Our customer service team and scientifically trained personnel will be pleased to help you create a unique compound... Show more »

In addition to our off-the-shelf range of Tocriscreen compound libraries, we offer a custom compound library service. Our customer service team and scientifically trained personnel will be pleased to help you create a unique compound library, designed to meet your exact screening requirements.

Tailor Your Library

  • Select compounds from our list of available compounds
  • Specify your desired quantities
  • Choose between dry/solid format or in DMSO
  • Stipulate the tube, vial, or plate to complement your compound handling system
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Antigen Expression and Purification

Price on request
Request a quote for more information about this service.

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Protein Formulation

Price on request

We have significant experience in formulating proteins for optimal stability and shelf-life, while maintaining biological activity.  Please write us a note on your specific project details and we'll discuss feasibility and if applicable a Statement of Work will be generated.  We have a range of buffer formulations... Show more »

We have significant experience in formulating proteins for optimal stability and shelf-life, while maintaining biological activity.  Please write us a note on your specific project details and we'll discuss feasibility and if applicable a Statement of Work will be generated.  We have a range of buffer formulations already and can assess the protein of interest and apply it to one of these buffer systems, and QC test.

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Antibody Modification

Price on request

Range of antibody modifications including antibody engineering, antibody deglycosylation, reformatting isotype, species switch, hinge region and Fc region edits.  Please inquire!

Range of antibody modifications including antibody engineering, antibody deglycosylation, reformatting isotype, species switch, hinge region and Fc region edits.  Please inquire!

1.   From scratch, de novo monoclonal and polyclonal antibody development using multiple host species options including goat, mouse, rat, rabbit, and more

2.   Conversion of hybridoma to recombinant antibody

3.   Custom recombinant antibody engineering services including Fab’2, scFv, inserting mutations, bi-specific antibodies, and truncated active forms.

4.   Generate in vitro transcribed Chimeric Antigen Receptor (CAR) mRNA transcripts using scFv cloning

5.   GMP-grade antibody, documentation, and cell line testing

6.   Production of antibody under serum-free conditions

7.   Immunogen design tailored to meet your project needs, spanning peptide immunizations to full-length protein

8.   Endotoxin purification to meet your project specifications

9.   Epitope mapping

10.  Antibody deglycosylation

11.  Reformatting antibody including isotype, species switch, hinge region, and Fc region

12.  Large scale production with affinity-based purification to Gram quantities

13.  Conjugation to fluorophores, HRP, and biotin

14.  Application testing services including Western Blot, Immunohistochemistry and Immunocytochemistry, Flow Cytometry, ELISA

15.  Molecular weight confirmation by SDS-PAGE, SEC analysis, endotoxin levels, direct ELISA

16.  Developing antibodies validated for our ProteinSimple platforms

17.  Multiple product formats available including liquid and lypophilized

18.  Purification choices with standard Protein A/G resins and antigen- specific affinity

19.  For almost every R&D Systems clone that we have on our website, we often have an additional panel of clones to the same target

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Protein Expression Screening and Optimization

Price on request

We are very successful in developing proteins, expression strategies, and purification; thousands of proteins both online and non-catalog.  Pilot expression can include CHO, NS0, HEK293, E.coli, depending on your protein of interest, and then we move foward with one cell line once we identify the right clone for... Show more »

We are very successful in developing proteins, expression strategies, and purification; thousands of proteins both online and non-catalog.  Pilot expression can include CHO, NS0, HEK293, E.coli, depending on your protein of interest, and then we move foward with one cell line once we identify the right clone for transfection, selection, screening, and banking.    Range of QC tests include but not limited to concentration, purity, endotoxin, N-terminal sequencing, microbiology, bioassay. 

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Polyclonal Antibody Production

Price on request

Range of polyclonal antibody production, including but not limited to goat and rabbit.  We will assist with antigen design, immunication, antibody purification, QC testing, cross-reacitivity testing, and formulation options.

Range of polyclonal antibody production, including but not limited to goat and rabbit.  We will assist with antigen design, immunication, antibody purification, QC testing, cross-reacitivity testing, and formulation options.

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Lyophilization

Price on request

Custom bottling and lyophilization services in a range of vial and bottling formats. 

Custom bottling and lyophilization services in a range of vial and bottling formats. 

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Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
Price on request
Request a quote for more information about this service.

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Animal Immunization for Antibody Production

Price on request
Request a quote for more information about this service.

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Antibody Purification

Price on request

Antibody purification via antigen affinity chromatography and range of techniques including Protein G.  Please inquire.

Antibody purification via antigen affinity chromatography and range of techniques including Protein G.  Please inquire.

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Cell-Based Assays

Price on request

We have over 900 cell-based bioassays, please contact us to discuss your next project. 

In addition, if looking for signaling events, our Proteome Profiler™ Antibody Arrays allow for the measurement of up to 119 proteins in a single sample. These arrays require no specialized equipment and eliminate the need... Show more »

We have over 900 cell-based bioassays, please contact us to discuss your next project. 

In addition, if looking for signaling events, our Proteome Profiler™ Antibody Arrays allow for the measurement of up to 119 proteins in a single sample. These arrays require no specialized equipment and eliminate the need for multiple Western blot experiments. Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies. The arrays utilize chemiluminescence for detection and membranes can be assessed for protein levels in the same manner as traditional Western blots. Select arrays are also suitable for use with the LI-COR® and FluorChem detection systems.

 

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Immunoassays

Price on request

R&D Systems ELISA's are the most trusted, most published ELISA's on the market with a menu of 700 target analytes spanning 12 species.  We have a range of immunoassay options spanning unique model organisms to murine to human species. 

Our master calibrated... Show more »

R&D Systems ELISA's are the most trusted, most published ELISA's on the market with a menu of 700 target analytes spanning 12 species.  We have a range of immunoassay options spanning unique model organisms to murine to human species. 

Our master calibrated Quantikine® assays ensure that our kits perform consistently over time. Results obtained today are comparable to results obtained last month or even last year.

Our Assays have controls with assigned ranges, guaranteeing performance.
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Recombinant Antigen Development

Price on request

R&D Systems brand generates full-length, bioactive proteins.  In addition, if your project requires, we can develop smaller domains of proteins if you may need to generate antibodies directed towards a specific epitope recognition sequence.  Message us to discuss your next antigen development project, we may... Show more »

R&D Systems brand generates full-length, bioactive proteins.  In addition, if your project requires, we can develop smaller domains of proteins if you may need to generate antibodies directed towards a specific epitope recognition sequence.  Message us to discuss your next antigen development project, we may already have something in our own freezers to save you time and money.  We will assess with you feasibility if it may be a custom, from scratch recombinant antigen development project. 

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Surface Plasmon Resonance (SPR)

Price on request

Biacore T200 SPR sample testing.  Please inquire for price per sample testing.  Lead time from sample receipt to data report is typically less than 10 days. 

Biacore T200 SPR sample testing.  Please inquire for price per sample testing.  Lead time from sample receipt to data report is typically less than 10 days. 

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BH

Brandon Hanna

Senior Key Account Manager
MR

Martin Ramsden

Commercial Development Manager

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