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Quick Biology Inc.

Pasadena, California, US

Quick Biology Inc. is located in Pasadena, CA. with a focus on developing new technologies to allow scientists/physicians to obtain more results in less time. We provide NGS service as well as related reagents to investigate the functions of identified variants. We also help physicians to unleash the power of NGS in disease diagnosis.

★We are equipped with the latest Illumina NovaSeq 6000, HiSeq X Ten, HiSeq 4000, NextSeq500, MiSeq and Ion Proton.
We have:
--High sequencing throughput and capacity (NovaSeq 6000 S2 flow cell, ~3300M reads of PE150);
--Fast turn around time (our HiSeq4000 runs twice a week on a routine basis);
--Very competitive price in RNA-seq, ATAC-seq, Whole Exome Seq and Whole Genome Seq;
--Advanced bioinformatic analysis, including analysis for RNA splicing/RNA editing;
--High-quality pictures for publication purpose

★We also provide functional genomic screening service using our... Show more »

Quick Biology Inc. is located in Pasadena, CA. with a focus on developing new technologies to allow scientists/physicians to obtain more results in less time. We provide NGS service as well as related reagents to investigate the functions of identified variants. We also help physicians to unleash the power of NGS in disease diagnosis.

★We are equipped with the latest Illumina NovaSeq 6000, HiSeq X Ten, HiSeq 4000, NextSeq500, MiSeq and Ion Proton.
We have:
--High sequencing throughput and capacity (NovaSeq 6000 S2 flow cell, ~3300M reads of PE150);
--Fast turn around time (our HiSeq4000 runs twice a week on a routine basis);
--Very competitive price in RNA-seq, ATAC-seq, Whole Exome Seq and Whole Genome Seq;
--Advanced bioinformatic analysis, including analysis for RNA splicing/RNA editing;
--High-quality pictures for publication purpose

★We also provide functional genomic screening service using our proprietary Drug Array and siRNA Array to conduct functional studies on your pathway of interest through drug-drug and drug-gene interactions on your cell line of interest.
(1) Drug Array:
- This Drug Array comprises 66 highly selective anti-cancer drugs, which allows users to quickly screen potential drug-drug and drug-gene interactions. It serves as a great tool to survey synthetic lethality for cancer treatment. It also serves as a tool to probe pathway-pathway interactions.
(2) siRNA Array
- siRNA array for Drug Targets: This array set silences 60 well-known drug targets, which allows users to screening potential gene-gene and gene-drug interaction.
- siRNA array for Cancer Driver Genes: This array set silences 60 well-known cancer driver genes.
Please contact us for a quote.
***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

Recent Publication:
Schlieve, C. R., Fowler, K. L., Thornton, M., Huang, S., Hajjali, I., Hou, X., … Grikscheit, T. C. (2017). Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine. Stem Cell Reports, 9(3), 883–896. http://doi.org/10.1016/j.stemcr.2017.07.017

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Quick Biology Inc. has not listed any services.

RNA Methylation Assays
Price on request

MeRIP-Sequencing (RNA Methylation)

Alternative names: RNA Methylation Seq, MeRIP-Seq, N6-Methyladenosine Seq, M6A RNA-seq

RNA methylation is a reversible post-translational modification to RNA that epigenetically impacts numerous biological processes. It occurs in different RNAs including tRNA, rRNA, mRNA, tmRNA, snRNA,... Show more »

MeRIP-Sequencing (RNA Methylation)

Alternative names: RNA Methylation Seq, MeRIP-Seq, N6-Methyladenosine Seq, M6A RNA-seq

RNA methylation is a reversible post-translational modification to RNA that epigenetically impacts numerous biological processes. It occurs in different RNAs including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases.

N6-methyladenosine (m6A) represents an abundant RNA modification that is conserved across many different species, ranging from plants, to yeast, to mammals. Similar to the 5-methylcytosine (5-mc) modification of DNA, m6A is a reversible chemical modification of RNA. m6A RNA modifications are an emerging topic in controlling cell fate transitions in mammalian embryonic stem cells. m6A is one of the most prevalent modifications on both mRNAs and noncoding RNAs in eukaryotes with an estimated 12,000 m6A sites in over 7,000 genes.

5-methylcytosine (5-mC) is an epigenetic mark that also commonly occurs in various RNA molecules. Researchers are able to bisulfite convert RNA, perform RT-PCR amplification, cloning, and sequencing to gain information about RNA cytosine methylation states. By treating RNA with bisulfite, cytosine residues are deaminated to uracil while leaving 5-methylcytosine intact.

Quick Biology now offers both MeRIP/m6A-seq and 5-mC RNA Bisulfite-Seq, please contact us to learn more!

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Illumina HiSeq 4000
Cancer
Illumina
RNA sequencing
Methylation
stem cell medicine
Chromosome Conformation Capture
Price on request

Hi-C Sequencing

Please inquire about our new Hi-C seq service.

The three-dimensional (3D) conformation of genomes has a profound impact on gene regulation, DNA replication, and DNA damage repair. Recent years have seen a drastic expansion of genome conformation mapping technologies, including Hi-C, a sequencing-based... Show more »

Hi-C Sequencing

Please inquire about our new Hi-C seq service.

The three-dimensional (3D) conformation of genomes has a profound impact on gene regulation, DNA replication, and DNA damage repair. Recent years have seen a drastic expansion of genome conformation mapping technologies, including Hi-C, a sequencing-based assay designed to interrogate the 3D organization
of the genome. While existing Hi-C protocols have served as a tremendously valuable tool for some in the community, widespread adoption has been precluded by labor-intensive, cumbersome and complex protocols, prolonged ~4 day duration, inconsistent experimental results, and the demand for billions of sequence reads per sample for high-resolution chromatin looping analyses.

Quick Biology is now implementing a streamlined and simplified Hi-C sequencing workflow that produces high-quality libraries with consistency. The high quality and enriched signal-to-noise HiC library enables the identification of both known and previously unknown chromatin interaction loops at much reduced sequencing depths.

We are running a pre-launch trials now and will accpet projects with discounted pricing. Please inquire about our Hi-C seq service.

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Illumina HiSeq X Ten
Illumina HiSeq 4000
Cancer
Genomics
Cell Line
Illumina NextSeq 500
Cultured Cells
Epigenomics
Illumina NovaSeq 6000
RNA Sequencing
Price on request

RNA seq


Sample requirements:

1) Total RNA amount: ≥ 1 ug for normal input; minimum 200 ng RNA input. Suspend RNA in RNase-free H2O.
2) Minimum sample concentration should be ≥ 20 ng/μl regularly.
3) RIN value ≥ 6.5 for plants and... Show more »

RNA seq


Sample requirements:

1) Total RNA amount: ≥ 1 ug for normal input; minimum 200 ng RNA input. Suspend RNA in RNase-free H2O.
2) Minimum sample concentration should be ≥ 20 ng/μl regularly.
3) RIN value ≥ 6.5 for plants and fungi, RIN value ≥ 7.0 for human and animals.
4) Purity: OD260/280 = 1.8-2.2, OD260/230 ≥ 2.0 without degradation or DNA contamination. Evaluate RNA integrity using a 1% denaturing RNA gel or an Agilent Technologies 2100 Bioanalyzer. Please submit your gel image or Agilent Bioanalyzer trace for your samples.
5) RNase-free screw-cap microcentrifuge tubes should be used for storage and shipping. Please label your samples clearly.

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

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Illumina NextSeq 500 sequencing system
Illumina HiSeq 4000
Illumina HiSeq 2500
illumina hiseq 4000
Illumina NextSeq
Illumina Novaseq
Single Cell RNA-Seq
Price on request

We are specialized in doing single-cell (low-input) RNA-seq library preparation for FFPE/degraded/low-input samples including single cells. Single-cell methods include whole transcriptome amplification (WTA), Chromium 10X and UPX 3' single-cell mRNA-seq. Please ask for a quote.

Quick Biology’s single cell / low-input... Show more »

We are specialized in doing single-cell (low-input) RNA-seq library preparation for FFPE/degraded/low-input samples including single cells. Single-cell methods include whole transcriptome amplification (WTA), Chromium 10X and UPX 3' single-cell mRNA-seq. Please ask for a quote.

Quick Biology’s single cell / low-input RNA-seq service (scRNA-seq) Option delivers:

Option #1 (low input/single-cell RNA-seq):
· Transcript discovery and differential expression from single eukaryotic cells and RNA-seq from limited samples including viral RNA.
· An all-in-one, direct cell-to-library solution incorporating robust whole transcriptome amplification (WTA) and highly efficient library preparations
· Completely PCR-free cell-to-library protocol to minimize bias and maximize transcript detection
· Libraries from single cells using a Quick Biology optimized streamlined protocol
· RNA-seq data includes both mRNA and lincRNA
· Fewer sequence errors – this is perfect for viral genome sequencing

Our scRNA-seq is suitable for transcriptome amplification for the analysis of:
(1) mRNA with poly A+ tails
(2) Total RNA
Note: Not recommended for tRNAs, miRNAs, severely degraded RNA, RNA from FFPE material or samples fixed by formaldehyde, glutaraldehyde or other fixatives.

Quick Biology scRNA-seq workflow:
We follow a complete WTA workflow from cell lysis, gDNA removal and cDNA synthesis to highly uniform amplification across the entire transcriptome in a one-tube protocol – with negligible sequence bias.

Sample requirements:
· 1–1000 intact cells from all vertebrate species (e.g., human, mouse, rat, sorted cells, tissue culture cells, cells picked under the microscope or microdissected cells from frozen tissue).
· 100 pg – 100 ng of purified RNA

Option #2: Chromium 10X single-cell transcriptome seq:
The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to tens of thousands of cells. Our latest improvements allow you to detect even more unique transcripts per cell and with the addition of Feature Barcoding technology, you can get a more complete molecular readout cell by cell—identify cell-specific CRISPR-mediated perturbations or simultaneously measure gene and cell surface protein expression in the same cell, with virtually unlimited possibilities for the additional feature types.
- Identify and characterize rare cell types
- Analyze and understand cellular heterogeneity and how this contributes to your biological system
- Perform cellular phenotyping with single cell RNA-seq to identify novel targets, biomarkers, and cell types and states without the need for pre-selected targets
- Evaluate mRNA and cell surface protein expression profiles within the same cell
- Perform high throughput and high resolution functional genetic screens in tens of thousands of cells simultaneously
- Assess comprehensive gene expression phenotypes for individual CRISPR perturbations

Option #3: Quick Biology’s UPX 3' single-cell mRNA-seq service (UPX 3' scRNA-seq) delivers:

  • For high-throughput 3' transcriptome NGS from ultralow amounts of RNA
  • Start with 1–100 cells or 10 pg to 1 ng of isolated RNA
  • LNA-enhanced chemistry for increased accuracy, specificity and sensitivity
  • UMIs eliminate library amplification bias for accurate gene expression
  • UPX tagging allows 4608–18,432 samples per single sequencing lane

Sample requirements:
· start with 1–100 cell
· 10 pg to 1 ng of isolated RNA

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Illumina HiSeq 4000
Illumina HiSeq 3000
Cancer
FFPE tissue
Biology
Drug Discovery
Illumina MiSeq
Illumina
RNA-Seq
RNA
Genomics
Cell Line
10x Genomics Chromium System
single cell transcriptome
Epigenetics
Low Input/Single Cell Whole Genome Sequencing
Price on request

Please check out our 2018 new service Single-cell/Low-input Whole Genome Sequencing!

We provide a complete solution for whole-genome sequencing from isolated single animal or bacterial cells or low amounts of genomic DNA (1–1000 intact cells, or >50 pg purified gDNA). This service is ideally suited to the analysis of... Show more »

Please check out our 2018 new service Single-cell/Low-input Whole Genome Sequencing!

We provide a complete solution for whole-genome sequencing from isolated single animal or bacterial cells or low amounts of genomic DNA (1–1000 intact cells, or >50 pg purified gDNA). This service is ideally suited to the analysis of aneuploidy and copy number variation and sequence variation in single cells or for whole genome sequencing from rare samples.

Applications:
- Analysis of inter-cellular genome heterogeneity
- Mutation detection
- Copy number variation analysis
- Aneuploidy analysis

Obtain whole genome sequences with comprehensive genome coverage and exceptional sequence fidelity from single cells or low input gDNA.
The proliferation of technologies that enabled the analysis of single, isolated cells has transformed research in neuroscience, cancer, reproduction and more. With technological breakthroughs in single cell isolation, whole genome amplification (WGA) and NGS library preparation, experiments using single cells are now possible – opening a wealth of exciting new insights for you to discover. However, challenges still exist. In particular, methods for the unbiased and complete amplification of a single genome and for the efficient conversion of that amplified DNA into a sequencer-compatible library face several technical limitations including incomplete amplification, the introduction of PCR errors, GC-bias and locus or allelic drop-out.
With these considerations in mind, Quick Biology offers optimized solutions for your research with the new low input / single cell whole genome sequencing (WGS) service.

Quick Biology’s single cell / low-input WGS service (scWGS) delivers:
• High sensitivity: Typical yield is greater than 5 µg when starting with 1 ng of genomic DNA. Whole genome amplification from as low as 6 pg DNA (5 human cells).
• Reduced amplification bias and no primer artefacts: Primer-free amplification method utilizes a combination of TthPrimPol Primase (to synthesize primers) and Phi29 DNA polymerase.
• Obtain higher genome coverage and greater sequence fidelity.

Quick Biology scWGS workflow:
We follow a complete WTA workflow from cell lysis, cDNA synthesis, highly uniform amplification across the entire genome to the final Illumina Sequencing in an optimized protocol:
Sample requirements:
• 1–1000 intact cells from many species (e.g., human, mouse, rat, bacteria, sorted cells, tissue culture cells, cells picked under the microscope or microdissected cells from frozen tissue).
• 50 pg – 1 ng of purified gDNA, free of any contaminants.

Turnaround time
About 4 weeks, depends on the quality of submitted samples, one additional week for data analysis.

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Illumina HiSeq X Ten
Illumina HiSeq 4000
Cancer
Biology
Genomics
Illumina NextSeq 500
Exosomal RNA Sequencing
Price on request
Request a quote for more information about this service.
Whole Exome Sequencing (WES)
Price on request

Whole Exome Sequencing
Required at least 6 samples.
2*150 PE with Hiseq 4000
Depth 50x
Including capture/library preparation/Reads QC

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

Whole Exome Sequencing
Required at least 6 samples.
2*150 PE with Hiseq 4000
Depth 50x
Including capture/library preparation/Reads QC

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

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Illumina NextSeq 500 sequencing system
Illumina HiSeq 4000
Illumina Novaseq
Whole Genome Sequencing
Price on request

Human Whole Genome Sequencing (WGS) on HiSeq X Ten, 90Gb (~30x), Turn Around Time: 4-6 weeks, including:
(1) Library preparation & Sequencing (~30x coverage, ~90 Gb raw data /sample, (PE150, Q30 ≥ 80%))
(2) Analysis (Data quality control, Alignment with UCSC hg19, Variant (SNP, CNV, InDel and SV) calling, annotation and... Show more »

Human Whole Genome Sequencing (WGS) on HiSeq X Ten, 90Gb (~30x), Turn Around Time: 4-6 weeks, including:
(1) Library preparation & Sequencing (~30x coverage, ~90 Gb raw data /sample, (PE150, Q30 ≥ 80%))
(2) Analysis (Data quality control, Alignment with UCSC hg19, Variant (SNP, CNV, InDel and SV) calling, annotation and statistics; and Somatic variant detection if samples are paired).
(3) data delivery (raw FASTQ, and final report) via hard drive.


SAMPLE REQUIREMENTS:

1) DNA amount: ≥500 ng for normal input (PCR-free); minimum 200 ng
2) DNA concentration: ≥20 ng/μL, Optimal concentration: 20 ng/μl to 500 ng/μl in Tris-HCL (pH8.0).
3) Purity: A260/280:1.8-2.0, A260/230: ≥ 1.8, Intact DNA, RNA-free, and non-degraded.
4) DNase-free screw-cap microcentrifuge tubes should be used for storage and shipping. Please label your samples clearly.

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

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Human
Illumina HiSeq X Ten
ATAC-Seq
Assay for Transposase-Accessible Chromatin with High Throughput Sequencing
Price on request

We offer very competitive pricing on ATAC-seq (Assay for Transposase-Accessible Chromatin with high throughput sequencing), which is an alternative or complementary method to MNase-seq (sequencing of micrococcal nuclease sensitive sites), FAIRE-seq and DNAse-seq. It aims to identify accessible DNA regions, equivalent to DNase I... Show more »

We offer very competitive pricing on ATAC-seq (Assay for Transposase-Accessible Chromatin with high throughput sequencing), which is an alternative or complementary method to MNase-seq (sequencing of micrococcal nuclease sensitive sites), FAIRE-seq and DNAse-seq. It aims to identify accessible DNA regions, equivalent to DNase I hypersensitive sites.


SAMPLE REQUIREMENTS:
1. If sending cells, prepare a cell pellet containing at least 1 x 10^5 cells (2.0 x 10^6 cells recommended). Please provide total cell number on the sample sheet.
2. Wash the centrifuged cell pellet with phosphate buffered saline (PBS) at least once (recommend three times) and place the cell pellet in a 1 ml vial or tube.
3. Resuspend the pellet in Cryostor media (or a compatible freezing media with DMSO). Freeze the cell vials at 80°C overnight slowly in an isopropyl alcohol chamber (aka Mr. Frosty) or similar cooling device at a rate of -1 degrees C per minute.
4. Label sample tubes with your company/institution name, sample identifier (such as cell line and passage), date of submission, and number of cells.
5. Ship the cell vials on dry ice, please see the sample packing recommendations for details.

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Whole Genome Bisulfite Sequencing
Price on request

We also offer Whole-Genome Bisulfite Sequencing, please quote!

We also offer Whole-Genome Bisulfite Sequencing, please quote!

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Illumina HiSeq 4000
whole genome bisulfite sequencing
Illumina Novaseq
Circular RNA Sequencing
Price on request

We offer service in circular RNA sequencing for efficient detection of circular RNAs and their genomic location through NGS sequencing and bioinformatics analysis such as: identifying, annotating and visualizing circRNAs and comparing relative expression, De novo assembling novel circular RNA transcripts, and characterizing various of alternative,(back-)splicing events of circular RNAs, etc.

We offer service in circular RNA sequencing for efficient detection of circular RNAs and their genomic location through NGS sequencing and bioinformatics analysis such as: identifying, annotating and visualizing circRNAs and comparing relative expression, De novo assembling novel circular RNA transcripts, and characterizing various of alternative,(back-)splicing events of circular RNAs, etc.

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Illumina HiSeq
Small RNA Sequencing
Price on request

Quick Biology’s miRNA-seq service delivers:

· Comprehensive discovery and differential expression of miRNA from many sample types including biofluids, low input samples, FFPE and any tissue.
· Gel-free miRNA sequencing library prep from as little as 1 ng of total RNA
· Integrated Unique Molecular... Show more »

Quick Biology’s miRNA-seq service delivers:

· Comprehensive discovery and differential expression of miRNA from many sample types including biofluids, low input samples, FFPE and any tissue.
· Gel-free miRNA sequencing library prep from as little as 1 ng of total RNA
· Integrated Unique Molecular Indices (UMIs) enable quantification of individual miRNA molecules, eliminating PCR and sequencing bias
· miRNA-seq data includes both miRNAs and other small RNAs (piRNA)
· Bioinformatics solutions for differential expression analysis and discovery of novel miRNAs

Quick Biology’s miRNA-seq is suitable for small RNAs for the analysis of:
(1) Biofluids (such as serum, plasma, CSF and urine)
(2) Total RNA and miRNA
(3) Cells, fresh/frozen tissue and FFPE tissues
(4) Exosomes containing small RNAs

Sample requirements:
· Serum, plasma, cells, fresh/frozen tissue and FFPE tissues
· 100 ng – 500 ng (minimum 1 ng) of purified total RNA (please isolate RNA using kits that retain miRNA fractions)

Bioinformatics for miRNA Sequencing:

Standard Analysis:
a. Raw data QC and clean up
b. Alignment to a reference with mapping statistics
c. miRNA count, quantifications, and differential miRNA expression analysis
d. Downstream miRNA target analysis and clustering
f. Final project report (HTML) with analysis methods, publication-ready graphics, and references

Advanced Analysis:
Please inquire about Quick Biology's customized data analysis.

Turnaround time:
About 4 weeks, depends on the quality of submitted samples, one additional week for data analysis.

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Illumina NextSeq 500 sequencing system
Illumina NextSeq
ChIP-Seq
Chromatin Immunoprecipitation Sequencing
Price on request

We offer a very competitive price and the highest data quality in the market. Please quote.

ChIP-sequencing, also known as ChIP-Seq or ChIP-seq, is a method used to analyze protein interactions with DNA. It combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of... Show more »

We offer a very competitive price and the highest data quality in the market. Please quote.

ChIP-sequencing, also known as ChIP-Seq or ChIP-seq, is a method used to analyze protein interactions with DNA. It combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. ChIP-seq is used primarily to determine how transcription factors and other chromatin-associated proteins influence phenotype-affecting mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states. This epigenetic information is complementary to genotype and expression analysis.

Quick Biology is a professional ChIP-Seq provider and will ensure the customer to get the most out of the sequencing project.

Complete ChIP-seq from cells/tissue to sequence

  • Input Amount of Tissue/Cells:
    In general, the number of cells and tissues for each reaction can be 1 x 10^5 to 1 x 10^6 and 5 mg to 50 mg, respectively. For optimal preparation, the input amount should be 4 to 5 x 10^5 cells or 20 to 30 mg tissues so that the amount of DNA enriched from the ChIP reaction can range from at least 1 ng to 100 ng.
  • Starting Materials:
    Starting materials can include various tissue or cell samples such as cultured cells from a flask or plate, fresh and frozen tissues, etc.
  • Antibodies:
    Antibodies should be ChIP-grade in order to recognize fixed and native proteins that are bound to DNA or other proteins.

If submitting ChIP-DNA fragments:

Sample requirements
• DNA amount quantified by Qubit 2.0: ≥ 100ng; minimum 10 ng
• DNA concentration: ≥1 ng/μL
• Total volume: ≥10 μL

Bioinformatics for ChIP Sequencing:

Standard Analysis:
a.Raw data QC and clean up
b.Alignment to a reference with mapping statistics
c.Peaking calling with or without control samples
d.Gene assignment and peak annotation
e.Pathway Analysis
f. Final project report (HTML) with analysis methods, publication-ready graphics, and references

Advanced Analysis:
h.Motif analysis
i.clustering and visualization

Turnaround time:
~3-4 weeks depending on project requirements, one additional week for data analysis

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Illumina MiSeq sequencing system
Illumina NextSeq 500 sequencing system
Illumina HiSeq 4000
ChIP-Seq
Illumina
Epigenetics
Illumina Sequencing
Starting at $1,500.00 per lane

HiSeq X ten, PE150, yeild: >600 M reads; 90Gb of data
Hiseq 3000, SE50, yield: >300M reads; $1500 Per lane;
Hiseq 4000, PE150 , yield: >600 M reads; 90Gb; $2650 (was $3000) Per lane
NovaSeq 6000, S2 flow cell, yield: 6600M reads; 850–1000 Gb of data
NextSeq 500 options:
- PE 2x150 High Output (up to 800M... Show more »

HiSeq X ten, PE150, yeild: >600 M reads; 90Gb of data
Hiseq 3000, SE50, yield: >300M reads; $1500 Per lane;
Hiseq 4000, PE150 , yield: >600 M reads; 90Gb; $2650 (was $3000) Per lane
NovaSeq 6000, S2 flow cell, yield: 6600M reads; 850–1000 Gb of data
NextSeq 500 options:
- PE 2x150 High Output (up to 800M reads)
- PE 2x150 Mid Output (up to 260M reads)
- PE 2x75 High Output (up to 800M reads)
- PE 2x75 Mid Output (up to 260M reads)
- SR 1x75 High Output (up to 400M reads)
MiSeq v2 and v3


SAMPLE REQUIREMENTS:
• Please contact us for compatibility of the library, Please provide index table if it is pooled library of many samples.
• Library amount and size: ≥ 70 x 10-3 pmol, The insert fragment size should be no more than 600bp. Single peak should be observed.
• Library concentration: ≥ 5 nM (fragment size adjusted), with volume range of 15-100 uL in Tris-HCL (pH8.0). Please label conc. clearly in tubes.
• Please provide QC analysis results measured by one or several of the following methods: Qubit®, NanoDropTM, Gel Electrophoresis, Caliper LabChip GX or Agilent Bioanalyzer.
• DNase-free screw-cap microcentrifuge tubes should be used for storage and shipping. Please label your samples clearly.

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

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Agilent Bioanalyzer
Illumina HiSeq X Ten
Illumina HiSeq 4000
Illumina HiSeq 3000
qReal Time PCR
Illumina MiSeq
Illumina NextSeq 500
RNA-Seq Data Analysis
Starting at $170.00 per sample

Bioinformatic Service for RNA-seq
- Standard service:
a.Raw data QC and clean up
b.Alignment to a reference with mapping statistics
c.Gene and transcript-based quantitation, TPM/RPKM/FPKM-based quantitation, Raw hit count-based quantitation
d.Differentially Expressed Gene
... Show more »

Bioinformatic Service for RNA-seq
- Standard service:
a.Raw data QC and clean up
b.Alignment to a reference with mapping statistics
c.Gene and transcript-based quantitation, TPM/RPKM/FPKM-based quantitation, Raw hit count-based quantitation
d.Differentially Expressed Gene
e.Clustering
f.Gene Ontology
g.Pathway Analysis

  • Advanced service:
    i.Alternative pre-mRNA splicing
    j.RNA editing
    k.Antisense transcript
    l.Novel transcripts
    m.Fusion genes/transcript detection
    Final project report with analysis methods, publication-ready graphics, and references

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

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Illumina MiSeq sequencing system
Illumina NextSeq 500 sequencing system
Cufflinks
Bowtie
Cuffdiff
Illumina HiSeq 4000
Gene Pathway Analysis
Price on request
Request a quote for more information about this service.
DNA Sequencing Data Analysis
Price on request
Request a quote for more information about this service.
HLA Typing
Price on request

HLA typing with Illumina TruSight HLA sequencing panel v2.
The Sequencing Panel covers all commonly typed HLA loci, plus those with emerging relevance:
Loci: Target Sequence
HLA-A: 4.1 kb (entire gene)
HLA-B: 2.8 kb (exon 1 — intron 6)
HLA-C: 4.2 kb (entire gene)
HLA-DRB1/3/4/5: 4.6 — 5.1 kb (exon 2 — intron 4)
HLA-DQB1:... Show more »

HLA typing with Illumina TruSight HLA sequencing panel v2.
The Sequencing Panel covers all commonly typed HLA loci, plus those with emerging relevance:
Loci: Target Sequence
HLA-A: 4.1 kb (entire gene)
HLA-B: 2.8 kb (exon 1 — intron 6)
HLA-C: 4.2 kb (entire gene)
HLA-DRB1/3/4/5: 4.6 — 5.1 kb (exon 2 — intron 4)
HLA-DQB1: 6.9 kb (entire gene)
HLA-DPB1: 9.7 kb (exon 2 — 3' UTR)
HLA-DQA1: 7.3 kb (entire gene)
HLA-DPA1: 10.3 kb (entire gene)

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16S rRNA Sequencing
Price on request

Please inquire for a quote

Please inquire for a quote

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Sequencing Experimental Design
Starting at $25.00 per hour

Quick Biology is specialized in Sequencing projects.
We provide cost effective consultation on experimental design for sequencing project. Please send us the project details.

Quick Biology is specialized in Sequencing projects.
We provide cost effective consultation on experimental design for sequencing project. Please send us the project details.

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Illumina NGS Library Preparation
Starting at $250.00 per sample

Illumina library preparation for RNA-seq, exome-seq, ChIP-seq and whole genome sequencing.
Start from $200 per sample.

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

Illumina library preparation for RNA-seq, exome-seq, ChIP-seq and whole genome sequencing.
Start from $200 per sample.

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

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NGS Data Analysis
Next generation sequencing data analysis
Starting at $170.00 per sample

$170/sample (was $250/sample);
Our advanced bioinformatics analysis will guide your research smoothly from sample data to discovery. While other companies deliver massive amounts of NGS data in Excel or text file formats, we go much further with in-depth bioinformatics analysis and the delivery of analysis results in user... Show more »

$170/sample (was $250/sample);
Our advanced bioinformatics analysis will guide your research smoothly from sample data to discovery. While other companies deliver massive amounts of NGS data in Excel or text file formats, we go much further with in-depth bioinformatics analysis and the delivery of analysis results in user project relevant context.

***: All information here is subject to change without notice. Please contact Quick Biology for latest pricing and terms.

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Illumina MiSeq sequencing system
Illumina NextSeq 500 sequencing system
Cufflinks
Bowtie
Cuffdiff
Illumina HiSeq 4000
GATK
Bioinformatics
Starting at $50.00 per hour

Professional bioinformatics support has become an essential part of any NGS based Genomics study. Quick Biology’s bioinformatics service is designed to assist researchers in areas of difficulties, such as Bio-IT, statistics, and data mining.

We perform also perform microarray data analyses, which can be combined with NGS... Show more »

Professional bioinformatics support has become an essential part of any NGS based Genomics study. Quick Biology’s bioinformatics service is designed to assist researchers in areas of difficulties, such as Bio-IT, statistics, and data mining.

We perform also perform microarray data analyses, which can be combined with NGS results. A lot of microarray data can be downloaded from public repository such as GEO and ArrayExpress.

Unique bioinformatics services from Quick Biology:

1.RNA-seq
a.Raw data QC and clean up
b.Alignment to a reference with mapping statistics
c.Gene and transcript-based quantitation, TPM/RPKM/FPKM-based quantitation, Raw hit count-based quantitation
d.Differentially Expressed Gene
e.Clustering
f.Gene Ontology
g.Pathway Analysis
h.Gene interaction network
i.Alternative pre-mRNA splicing
j.RNA editing
k.Antisense transcript
l.Novel transcripts
m.fusion genes/transcript detection
n.Final project report with analysis methods, publication-ready graphics, and references

2.Whole Exome Sequencing
a.Germline variants, compared to reference genome
b.Somatic mutations if Tumor-Normal pair (SNVs, InDel, CNV)

3.Whole Genome Sequencing
a.Germline variants, compared to reference genome
b.Somatic mutations if Tumor-Normal pair

4.ChIP Sequencing
a.Raw data QC and clean up
b.Alignment to a reference with mapping statistics
c.Peaking calling with or without control samples
d.Gene assignment and peak annotation
e.clustering and visualization
f.Motif analysis
g.Pathway Analysis
h.Gene interaction network analysis
i.Final project report with analysis methods, publication-ready graphics, and references

5.Combinatorial/Integrative Genomics and customized service
-Combinatorial
a.Combine with Cancer Genome Atlas: TCGA)
b.Combine with data in GEO (Gene Expression Omnibus)
c.Combine with data in ArrayExpress
d.Combine with data in Cosmic
e.Combine with data in GDSC
f.Combine with data in CCLE
-Integrative Genomics and customized service
g.Sequential analysis combining several distinct omics data from same set of samples.
h.Gene-set analysis to discover novel or using known groups of related genes/molecules.
i.Pairwise correlation analysis to infer molecular network interactions.
j.Network analysis using molecular network interactions to identify active or aberrant subgraphs.
k.Bayesian analysis to guide making correct assumptions to integrate multiple omics data.
l.Integrative analyses of many tumor types for clinical application.

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MicroRNA (miRNA) Sequencing
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DNA Extraction and Purification
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DNA Library Preparation
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Statistical Analysis
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Statistical Analysis Services

Statistical Analysis Services

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Data Analysis
Price on request

Data Analysis Services

Data Analysis Services

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Data Services
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Data Services

Data Services

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RNA Extraction and Purification
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DNA Sequencing
Price on request
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October 31, 2018

Positive review received for RNA Sequencing:

"The team at Quick Biology completed my order in good time and were very patient when there were delays in getting the samples to them. I am very satisfied with their level of engagement and service and would definitely work with them again."

October 25, 2018

Positive review received for Illumina Sequencing:

"Eric Liao and all the team from Quick Biology were very friendly and helpfull in our first experience in Science Exchange. We are satisfied with the quality of their work and we definitly will look for Quick Biology again should we need it."

2018-10-17 10:14:16 -0400

Net Promoter Score of 9 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-10-16 02:28:40 -0400

Net Promoter Score of 7 received for ATAC-Seq.

Additional Ratings: satisfaction with deliverable: 6, satisfaction with timeliness: 6.
2018-10-12 14:28:46 -0400

Net Promoter Score of 8 received for ATAC-Seq.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-10-10 18:56:25 -0400

Net Promoter Score of 10 received for Illumina Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-10-10 07:53:49 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
October 10, 2018

Positive review received for RNA Sequencing:

"Great sequence service and good customer service. They always keep in touch, giving feedback about the processes status. Recommended"

2018-10-09 19:28:08 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-10-09 07:42:36 -0400

Net Promoter Score of 10 received for ATAC-Seq.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-09-19 16:07:35 -0400

Net Promoter Score of 10 received for Illumina Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-09-18 19:17:38 -0400

Net Promoter Score of 8 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
2018-09-13 11:09:30 -0400

Net Promoter Score of 10 received for Illumina Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-08-30 15:04:22 -0400

Net Promoter Score of 3 received for ATAC-Seq.

Additional Ratings: satisfaction with deliverable: 6, satisfaction with timeliness: 7.
2018-08-21 16:54:26 -0400

Net Promoter Score of 10 received for Whole Exome Sequencing (WES).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-07-30 18:20:41 -0400

Net Promoter Score of 10 received for Illumina Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-06-28 10:53:40 -0400

Net Promoter Score of 10 received for Whole Genome Sequencing.

2018-06-18 15:48:22 -0400

Net Promoter Score of 8 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 8.
2018-06-07 23:01:56 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-06-04 09:45:17 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-05-11 12:31:17 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-04-24 15:46:02 -0400

Net Promoter Score of 9 received for Small RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-04-16 00:34:02 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-03-15 19:26:17 -0400

Net Promoter Score of 10 received for Low Input/Single Cell RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-03-06 01:15:28 -0500

Net Promoter Score of 9 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-02-16 11:48:49 -0500

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
February 3, 2018

Positive review received for Whole Exome Sequencing (WES):

"High quality data, finished on time. Very knowledgeable. Will definitely work with Quick Biology again for sequencing."

2018-01-30 11:25:55 -0500

Net Promoter Score of 10 received for Whole Exome Sequencing (WES).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-01-19 15:21:46 -0500

Net Promoter Score of 10 received for Whole Genome Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-01-15 21:15:04 -0500

Net Promoter Score of 7 received for ATAC-Seq.

Additional Ratings: satisfaction with deliverable: 6, satisfaction with timeliness: 7.
2017-12-21 12:43:56 -0500

Net Promoter Score of 9 received for Whole Genome Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 8.
2017-12-18 22:03:07 -0500

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
2017-12-15 17:32:11 -0500

Net Promoter Score of 10 received for RNA Sequencing.

2017-12-13 14:05:49 -0500

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-12-07 14:02:37 -0500

Net Promoter Score of 8 received for Small RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2017-12-05 10:36:38 -0500

Net Promoter Score of 6 received for ATAC-Seq.

Additional Ratings: satisfaction with deliverable: 6, satisfaction with timeliness: 5.
2017-11-22 23:44:46 -0500

Net Promoter Score of 7 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 7.
October 18, 2017

Positive review received for RNA Sequencing:

"It was an absolute pleasure to work with Quick Biology. The start to finish the project went very smoothly. I was very pleased with the data analysis that was provided and was taken aback by the price."

2017-10-16 11:09:03 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-10-12 13:24:29 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-10-11 13:02:40 -0400

Net Promoter Score of 10 received for Whole Genome Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
September 28, 2017

Positive review received for 16S rRNA Sequencing:

"Responsive to requested time-line."

2017-09-26 15:25:09 -0400

Net Promoter Score of 9 received for 16S rRNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2017-09-13 22:19:18 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-08-31 16:21:35 -0400

Net Promoter Score of 10 received for NGS Data Analysis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-08-25 21:02:20 -0400

Net Promoter Score of 7 received for Small RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 7, satisfaction with timeliness: 9.
August 25, 2017

Positive review received for Small RNA Sequencing:

"Professional team, the guys we were working with were quick and really wanted to help"

2017-08-24 11:36:39 -0400

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
August 16, 2017

Positive review received for RNA Sequencing:

"Fast & High-Quality Services."

June 26, 2017

Positive review received for RNA Sequencing:

"Quick Biology did great job, especially regard the technique support. Tim is very knowledgeable and patient on interpretation of the data. Thanks. Mei Hua"

May 11, 2017

Positive review received for RNA Sequencing:

"Overall service is satisfied. Although due to their sequencing machine problem, it took one more week to deliver the data and result. The data is pretty reliable."

2017-02-24 13:23:58 -0500

Net Promoter Score of 10 received.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 10.
2017-02-23 13:18:38 -0500

Net Promoter Score of 10 received for RNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2016-09-29 11:52:31 -0400

Net Promoter Score of 10 received for Fluorescence in situ Hybridization (FISH).

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
August 29, 2016

Positive review received for RNA Sequencing:

"Work was well done and I got very good deep sequencing with good concordance between replicates. The response was fast and received the fastq data the next day on a hard drive. I will definitely use them again in the future."

2016-08-10 15:56:06 -0400

Net Promoter Score of 9 received for RNA Sequencing.

August 6, 2016

Positive review received for Sequencing Experimental Design:

"I had several orders with Quick Biology and never had a issue. They are very knowledgeable and helpful, analyzed my data and answered my questions in a timely manner."

July 21, 2016

Positive review received for Bioinformatics:

"It is a wonderful experience to work with Quick Biology. Will purchase the service again."

July 21, 2016

Positive review received for Bioinformatics:

"I like their bioinformatic service for my RNA-Seq data."

July 15, 2016

Positive review received for Sequencing Experimental Design:

"Very experienced in NGS. Will work with Quick Biology again."

July 15, 2016

Positive review received for Sequencing Experimental Design:

"I like their quick service."

July 14, 2016

Positive review received for RNA Sequencing:

"The price is unbeatable and the service is excellent. We got our RNA sequencing data within 4 weeks!"

2016-06-21 18:24:15 -0400

Net Promoter Score of 9 received for Bioinformatics.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 9.
2016-06-20 20:09:58 -0400

Net Promoter Score of 9 received for Sequencing Experimental Design.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
June 20, 2016

Positive review received for RNA Sequencing:

"Quick Biology is real "quick". Eric Liao has been super helpful."

June 14, 2016

Positive review received for Illumina Sequencing:

"Great experience overall. Will definitely do the next round of sequencing with Quick Bio. Highly recommend."

April 1, 2016

Positive review received for Bioinformatics:

"Timely and responsible. Good quality service overall."

March 29, 2016

Positive review received for RNA Sequencing:

"Will definitely work with Quick Biology again. I am impressed by their quick turn around time."

March 29, 2016

Positive review received for Bioinformatics:

"Had a really good experience with Quick Biology. They deliver their service fast."

March 12, 2016

Positive review received for Sequencing Experimental Design:

"I am a returning customer and I like their service."

March 6, 2016

Positive review received for Bioinformatics:

"good services. It will provide more help for my experiments."

March 6, 2016

Positive review received for Bioinformatics:

"The service is excellent. Will collaborate with Quick Biology again."

March 6, 2016

Positive review received for NGS Data Analysis:

"The service is excellent. Will collaborate with Quick Biology again."

March 5, 2016

Positive review received for Bioinformatics:

"Good service! Help me a lot!"

March 1, 2016

Positive review received for Bioinformatics:

"Quick response and very professional on bioinformatics. Completed my analyses in a timely manner. Will definitely work with Quick Biology again."

March 1, 2016

Positive review received for Bioinformatics:

"I contacted quick biology for a bioinformatic analysis which is not listed in their service packages. Quick Biology team still replied professionally and got the my request done in time. I think they are not selling their products, but really helping biologists solve problems."

February 29, 2016

Positive review received for Bioinformatics:

"I really like the bioinformatics services, the cost dependents what analysis is done. Especially for the wet lab, I think this is very efficient way for pilot large biological data mining. Quick biology did a great job."

February 27, 2016

Positive review received for Bioinformatics:

"very good product. It will make experient quick, accuracy."

February 26, 2016

Positive review received for Bioinformatics:

"You helped me a lot since I don't know much about bioinformatics. Awesome!"

February 25, 2016

Positive review received for Bioinformatics:

"will definitely work with Quick biology again. They did excellent job and delivered all the information in the way I wanted."

February 22, 2016

Positive review received for Bioinformatics:

"Quick biology did a great job. They really took care of my projects, worked very efficiently."

February 9, 2016

Positive review received for Bioinformatics:

"Will definitely work with Quick Biology again. The company responded quickly and completed the work as expected."

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2016-09-24 01:29:33 +0800

I am a PhD Candidate at Worcester Polytechnic Institute. I found Quick Biology from the Science Exchange. I was originally attracted to the very reasonable price for their NGS services. However, their great customer service, very fast turnaround, and high quality of the bioinformatics analysis we have received is what will keep me loyal to Quick Biology.

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2016-03-29 15:03:02 +0800

I am a research scientist in cancer biology, and I get to know Quick Biology from my colleague. No repeating anything about their reasonable NGS price. The impressive thing I really want to share with other researchers is that: I sent the request into wrong place in Quick biology Science Exchange link, think what happened? Instead of attaching my contact information, forwarding my request into their different department, and letting customers wait for 1-2 more business days, Quick biology team did internal coordination, directly answered my questions in one message. I think many big life science companies should learn this from Quick Biology ---------no bureaucracy will have higher efficiency.

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2016-03-06 22:56:53 +0800

It is a pleasure to endorse Quick Biology. I am a faculty at Harvard Medical School and get to know Quick Biology from a friend. I am really impressed by their service in Next Generation Sequencing and related Bioinformatics. The price is affordable, response is quick and service is excellent.

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2016-03-03 02:10:48 +0800

I am an Assistant Professor at Cedars-Sinai and have used RNA-seq service from Quick Biology. We obtained very nice results and are satisfied with their service. My friend recommended Quick Biology to me and I would like to forward this recommendation to these using Next Generation Sequencing in their labs.

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2016-03-02 03:16:46 +0800

I have an excellent experience with Quick biology. Actually, I randomly browsed Science Exchange bioinformatics services and contacted quick biology for a bioinformatic analysis which is not listed in their service packages. Quick Biology team still replied professionally and got the my request done in time. I think they are not selling their products, but really helping biologists solve problems.

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2016-03-01 07:08:51 +0800

I know Quick Biology from science exchange website. I did two bioinformatic services requests, Quick biology team always replied my questions quickly, finished the analysis within time. One more thing, the affordable price :)

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2016-02-17 12:30:23 +0800

I did business with this company and was very satisfied with their service

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2016-02-12 09:53:03 +0800

I came to know Quick Biology from Science Exchange. Their drug array product has a clever design and covers most know cancer-pathways, and it helped me to uncover important drug-cancer interaction in cancer cells. Their product is also affordable.

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2016-02-10 14:42:58 +0800

Get to know Quick Biology from a friend. Ordered bioinformatic service to process my data. The Quick Biology staff responded quickly and address all my questions. They are very professional. Would like to recommend Quick Biology to all users on this platform.

Endorse this lab