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QPS Neuropharmacology

8 Orders Completed
Grambach, AT

About QPS Neuropharmacology

Divison of: QPS Holdings, LLC

QPS Neuropharmacology is the division of QPS that focuses on preclinical studies in CNS diseases, Orphan Diseases and Mental Disorders. The on-site availability of highly predictive disease models and unparalleled experience with studies performed for biopharmaceutical companies of all sizes makes QPS Neuropharmacology the first choice for most CNS drug development needs.

Validated transgenic and non-transgenic in vitro and in vivo models cover most targets of Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal Dementia (FTD), Niemann-Pick Disease, Autism Spectrum Disorder (ASD), Schizophrenia, Lewy Body Dementia (LBD) and other neurodegenerative diseases.


Recent Publications

  • Neurofilament-Light Chain as Biomarker of Neurodegenerative and Rare Diseases With High Translational Value. Tina Loeffler, Irene Schilcher, Stefanie Flunkert, and Birgit Hutter-Paier. Front. Neurosci., 11 June 2020.
  • Transgene integration causes RARB downregulation in homozygous Tg4-42 mice. Hinteregger B, Loeffler T, Flunkert S, Neddens J, Birner-Gruenberger R, Bayer TA, Madl T, Hutter-Paier B. Sci Rep. 2020 Apr 14;10(1):6377. doi: 10.1038/s41598-020-63512-8.
  • Correlation of Pyroglutamate Amyloid β and Ptau Ser202/Thr205 Levels in Alzheimer’s Disease and Related Murine Models. Joerg Neddens, Magdalena Daurer, Stefanie Flunkert, Kerstin Beutl, Tina Loeffler, Lauren Walker, Johannes Attems, Birgit Hutter-Paier. PLoS One. 2020 Jul 9;15(7):e0235543. doi:10.1371/journal.pone.0235543. eCollection 2020.
  • Characterization of the visceral and neuronal phenotype of 4L/PS-NA mice modeling Gaucher disease. Schiffer V., Santiago-Mujika E., Flunkert S., Schmidt S., Farcher M., Loeffler T., Schilcher I., Posch M., Neddens J., Sun Y., Kehr J., Hutter-Paier B. PLoS One. 2020 Jan 13;15(1):e0227077. doi: 10.1371/journal.pone.0227077. eCollection 2020.
  • Hepatic and neuronal phenotype of NPC1−/− mice. Santiago-Mujica E, Flunkert S, Rabl R, Neddens J, Loeffler T, Hutter-Paier B. Heliyon. 2019 Mar 14;5(3):e01293. doi: 10.1016/j.heliyon.2019.e01293.


    Certifications & Qualifications

    AAALAC

    Diversity Certificates

    Small business

    Our Services (113)


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    Rare Disease Animal Models

    Price on request

    QPS Neuropharmacology is experienced in generating, characterizing and maintaining of transgenic disease models and using them for drug testing projects for more than 15 years. We offer mouse and rat models available for drug development in many rare orphan diseases such as Huntington’s Disease (HD), Niemann-Pick Disease, Gaucher Disease, Amyotrophic Lateral Sclerosis (ALS), Alport Syndrome and Frontotemporal Lobar Degeneration (FTLD).

    Huntington’s Disease 

    • R6/2 transgenic Mouse Model
    • BACHD transgenic Rat Model


    Amyotrophic Lateral Sclerosis

    • TDP-43 transgenic Mouse Model
    • SOD1-G93A transgenic Mouse Model


    Frontotemporal Lobar Degeneration

    • TDP-43 transgenic Mouse Model
    • TAU transgenic Mouse Models


    Alport Syndrome

    • Col4A3-/- Mouse Model


    Lysosomal Storage Diseases

    Niemann-Pick Disease

    • NPC1-/- Mouse Model


    Gaucher Disease

    • 4L/PS-NA Mouse Model


    Mucopolysaccharidosis

    • MPS IIIA Mouse Model


    We are happy to receive any inquiry to elaborate the most efficient way, including behavioral, immunohistochemical, biochemical and molecular biological techniques, for the support of your drug development approach.

    Orphan Diseases Lysosomal Storage Disorders Gaucher Mucopolysaccharidosis Niemann-Pick Huntington's Disease Frontotemporal Lobar Degeneration Amyotrophic Lateral Sclerosis (ALS) CBE-induced Transgenic Mouse Mouse Show 11 more tags Show less

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    Biomarker Assay Development

    Price on request

    The expertise of QPS Neuropharmacology lies within the field of neurodegenerative diseases. We provide a state of the art research environment (AAALAC certified) for testing and evaluating new potential treatment approaches. Besides offering a broad spectrum of transgenic models and applications, we offer the establishment of new and customized assays that suit your special needs.

    Research Fields 

    • Alzheimer's Disease 
    • Parkinson's Disease  
    • Tauopathies   
    • Orphan Diseases   
    • Lysosomal Storage Diseases   
    • Psychiatric Diseases 
    • Inflammation
    • Applications

    Applications     

    • Gene & Protein Expression  
    • Enzyme Activity Assays 
    • Customized Cloning  
    • Cytokine Profile 
    • Lipid Profile
    • A4 Assay

    CNS/Neurology Neurodegeneration Orphan Diseases Rare Disease Alzheimers Disease Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Niemann-Pick Gaucher Alport Amyloid TAU SOD1 TDP43 Synuclein Cytokines Neurofilament Light Chain (NF-L) Show 17 more tags Show less

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    Animal Biobanking

    Price on request

    QPS Neuropharmacology’s well characterized and validated in vivo models are useful tools to push your CNS drug discovery research forward. We are happy to support your research activities with sample material from our biobank composed of various specimen derived from our in-house in vivo models. Tissues of transgenic and non-transgenic animals of various age groups are readily available such as brain tissue, CSF and plasma samples.

    Alzheimers Disease Parkinson's disease (PD) Blood Plasma FFPE tissue Frozen Tissue Brain CSF Mouse Mice CNS/Neurology Synuclein APP TAU Aggregation Show 15 more tags Show less

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    In vivo Drug Efficacy Testing

    Price on request

    As a leading CRO for CNS drug development, QPS Neuropharmacology is the premier provider for services with transgenic animals. The enterprise gained experience in generating, characterizing and maintaining transgenic disease models and using them for drug testing projects for more than 15 years.

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.


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    In vivo PK/PD Studies

    Price on request

    As a leading CRO for CNS drug development, QPS Neuropharmacology is the premier provider for services with transgenic animals. The enterprise gained experience in generating, characterizing and maintaining transgenic disease models and using them for drug testing projects for more than 15 years.

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.

    PK PK/PD in vivo pharmacology models pharmacodynamics pharmacokinetics Mouse Rat Show 7 more tags Show less

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    Animal Models and Studies

    Price on request

    QPS Neuropharmacology offers mouse and rat models for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases.


    The fully AAALAC accredited Animal Facility provides all in vivo services from mouse and rat breeding to delivering study reports that contain all experimental procedures and their outcome. Animals are housed under SPF conditions in IVC systems with periodic health monitoring. Our approach is based on a sequence of procedural building blocks that allow customization of any service request according to your specific needs. You have the option to select any entry point and exit point in our workflow.

    Neurodegenerative diseases Dementia Frontotemporal Lobar Degeneration Parkinson's disease (PD) Niemann-Pick Rare Disease Orphan Diseases psychiatric disorders behavioral assay Neuroscience neurobehavioral screening rats Mice Aging CNS/Neurology Show 15 more tags Show less

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    CNS/Neurology Animal Models

    Price on request

    QPS Neuropharmacology offers mouse and rat models for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases.


    The fully AAALAC accredited Animal Facility provides all in vivo services from mouse and rat breeding to delivering study reports that contain all experimental procedures and their outcome. Animals are housed under SPF conditions in IVC systems with periodic health monitoring. Our approach is based on a sequence of procedural building blocks that allow customization of any service request according to your specific needs. You have the option to select any entry point and exit point in our workflow.

    Alzheimers Disease Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Huntington's Disease Multiple sclerosis (MS) psychiatric disorders Lysosomal Storage Disorders Orphan Diseases Rare Disease Aging Anxiety Autism cognition Drug Discovery Biology Neuroscience Toxicology Pharmacology AAV testing animal model development Rat Model catheter lentivirus mouse breeding Mouse LPS MPTP- induced parkinson model rat breeding Research models and services transgenic animal model sprague dawley rat Mouse Rat rats 6-OHDA lesion model in rats Transgenic APP TAU Neurodegeneration CNS/Neurology Aging Psychiatry/Mental Health Show 41 more tags Show less

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    Animal Behavior Studies

    Price on request

    QPS Neuropharmacology provides a wide range of behavioral tests for various indications. Most tests are evaluated using rater-independent digital animal tracking systems.

    • General Health & Activity Levels
      • Cylinder Test
      • Glucose Tolerance Test
      • Irwin Test
      • Nest Building Test
      • Open Field Test
    • Cognitive Tests
      • Barnes Maze Test
      • Contextual Fear Conditioning Test
      • Morris Water Maze Test
      • Novel Object Recognition Test
      • Passive and Active Avoidance Test
      • Two Choice Swim Test
      • Y-Maze Test
    • Motor Ability Tests
      • Beam Walk and Challenging Beam Walk Test
      • Grip Strength
      • Pasta Gnawing Test
      • Pole Test
      • Rota Rod Test
      • Wire Hanging Test
    • Anxiety & Depression Tests
      • Elevated Plus Maze
      • Forced Swim Test
      • Marble Burying Test
      • Novelty-Induced Hypophagia (NIH) Test
    • Social Behavior Tests
      • Autogrooming Test
      • The Reeperbahn Test
      • Three Chamber Social Test
    • Lateral Lesion Tests
      • Corner Turn Test
      • Corridor Test
      • Rotation Test

    Can’t find what you are looking for? We are constantly establishing new tests and would be glad to develop tests according to your requirements.

    Alzheimers Disease Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Aging Lysosomal Storage Disorders Orphan Diseases Depression Anxiety Morris water maze Barnes maze cognition and memory cognitive decline Forced swim test health Monitoring Animal Movement Analysis Elevated plus maze rotarod Open field Pasta Gnawing Novel object recognition Marble burying test Show 21 more tags Show less

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    Alzheimer's Disease Animal Models

    Price on request

    QPS offers a variety of transgenic animal models for drug development in Alzheimer’s Disease (AD).

    APP Transgenic Mouse Models
       

    • APPsl   
    • APPsl x hQC
    • ApoB-100 
    • ApoB x APP
    • 5xFAD
    • Tg2576


    TAU Transgenic Mouse Models 


    • TMHT 
    • hTau   
    • PS19


    Scopolamine Treated Mice

    Alzheimers Disease CNS/Neurology Neurodegeneration Neurodegenerative diseases Neuroinflammation APP Amyloid TAU Aggregation Seeding Tau Seeding tau propagation scopolamine-induced amnesia model Show 13 more tags Show less

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    Parkinsons Disease Animal Models

    Price on request

    QPS Neuropharmacology offers a variety of transgenic and lesioned rodent models for drug development in Parkinson’s disease (PD).

    Alpha-Synuclein Transgenic Mouse Models
      

    • D-Line 
    • Line 61  
    • Models by MJFF


    Lesion Models:
      

    • MPTP Mouse Model
    • 6-OHDA Lesion Model


    Can’t find what you are looking for? We are constantly searching for new animal models and would be glad to characterize them for your drug development.

    Parkinson's disease (PD) Neurodegeneration Neurodegenerative diseases 6-OHDA unilateral 6-OHDA lesion MPTP Lesion model MPTP-induced Parkinson's disease model Animal Movement Analysis Synuclein LRRK2 Parkin Movement Disorder haloperidol-induced Parkinson's disease model catalepsy Show 14 more tags Show less

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    ALS Animal Models

    Amyotrophic Lateral Sclerosis Animal Models
    Price on request

    QPS Neuropharmacology offers a variety of transgenic mouse models for drug develoment in Amyotrophic Lateral Sclerosis (ALS). We have animals available for SOD1 and TDP43 pathology.
       

    • Human SOD1-G93A (high & low copy expressor mice)    
    • Human TDP-43 Transgenic TAR6/6 Mice   
    • Induced Human TDP-43 mice


    Can’t find what you are looking for? We are constantly searching for new animal models and would be glad to characterize them for your drug development.

    Amyotrophic Lateral Sclerosis (ALS) Transgenic Mouse SOD1 TDP43

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    Huntington's Disease Animal Models

    Price on request

    QPS Neuropharmacology offers a variety of transgenic rodent models for drug develoment in Huntington's Disease (HD).

    •    R6/2 transgenic Mouse Model
    •    zQ175 transgenic Mouse Model
    •    BACHD transgenic Rat Model


    Can’t find what you are looking for? We are constantly searching for new animal models and would be glad to characterize them for your drug development.

    Huntington's Disease Rare Disease Neurodegenerative diseases Neurodegeneration Animal Movement Analysis Rat Mouse Huntingtin HTT Show 9 more tags Show less

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    Inflammation Animal Models

    Price on request

    Neuroinflammation is a common feature of different neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s disease, Frontotemporal dementia, Amyotrophic lateral sclerosis and several others. Recent research suggests that targeting neuroinflammation might present a valid method to treat neurodegenerative diseases. In order to model neuroinflammation independent from other disease-relevant pathologies, wildtype mice can be peripherally injected with lipopolysaccharide (LPS).

    Mouse LPS Cytokines Neuroinflammation IL-1ß

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    Autism Animal Models

    Price on request

    The cause of Autism Spectrum Disorder (ASD) as a complex developmental disorder with highly varying degrees is still under investigation. Research suggests that multiple factors, such as genetic vulnerability and environmental factors are involved in the development of the disorder. QPS Neuropharmacology offers an extensive CRO service to test your compounds in the frequently used BTBR T+ tf/J mouse model for ASD.



    Autism Spectrum Disorder (ASD) mental disorders Autogrooming Social Deficits Social approach and recognition BTBR Mouse AChe CHAT Show 8 more tags Show less

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    Schizophrenia Animal Models

    Price on request

    QPS Neuropharmacology offers induced rodent models for drug develoment in Schizophrenia:

       

    • MK-801 Treated Mice 
    • AMPH or PCP Treated Rats


    Can’t find what you are looking for? We are constantly searching for new animal models and would be glad to establish new models for your drug development.

    mental disorders psychiatric disorders Open field Startle response Prepulse Inhibition (PPI) Phencyclidine (PCP) Amphetamine (AMPH) MK-801 NMDA receptor antagonists Show 9 more tags Show less

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    In vitro Inflammation Models

    Price on request

    Chronic neuroinflammation includes not only long-standing activation of microglia and subsequent sustained release of inflammatory mediators, but also the resulting increased oxidative and nitrosative stress.

    At QPS Austria currently 3 in vitro models for neuroinflammation based on LPS stimulation are established:

    • Immortalized Microglia Cell Lines
    • Primary Mouse Microglia
    • Organotypic Brain Slices

    LPS-stimulated organotypic hippocampal slices provide an optimal tool to investigate the impact of developmental compounds on inflammasome activation in vitro.
    By maintaining the three-dimensional structure and interplay of different cell types of the postnatal brain, this system closely resembles the in vivo situation while offering several advantages for early screenings:

    • Take supernatant samples anytime
    • Test new compounds without the challenge of BBB permeability
    • Semi high through-put
    • Dexamethasone can serve as reference compound

    Neuroinflammation Inflammasome NLRP3 IL-1ß Cytokines Murine Microglia Organotypic Brain Slices Hippocampal Slices caspase Show 9 more tags Show less

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    Organotypic Cell Culture

    Price on request

    Organotypic brain slice cultures are an innovative and robust in vitro method to study several cell types of the brain in a complex network. Slices can be cultured as single slices isolated from different brain regions or as whole brain slices.

    Organtoypic brain slices are your method of choice to investigate processes ex vivo with an intact 3D structure and cell-cell interactions. Especially hippocampal and whole brain slices are frequently used at QPS to study neuroinflammatory processes under highly physiological conditions.

    mRNA Protein LPS Cytokines

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    Neuronal Assays

    Price on request

    During development, neurons become assembled into functional networks by growing out axons and dendrites (collectively neurite outgrowth) that connect synaptically to other neurons. Strikingly, neurons retain their capacity for growth and synapse formation in the adult brain. Only those neurons that manage to establish adequate contacts with neighbor cells escape cell death. To stimulate and accelerate neurite outgrowth is thus important for restoring neuronal function after injury or from neurodegenerative diseases. A promising strategy for drugs targeted against neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease is promoting the regeneration of neurons. Thus, new therapies are focusing on identifying new molecules that affect neurite outgrowth. For this purpose, QPS Neuropharmacology provides cell culture models to assess the neurotrophic effects of your developmental compounds.

    Neuroscience

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    Cell and Tissue Culture

    Price on request

    QPS Neuropharmacology provides in vitro research services with numerous standardized cell culture systems including transgenic and non-transgenic cell lines, glial cells, primary chicken and rat peripheral and central nervous system neurons of different developmental stages and organotypic brain slices. New models are developed and validated on request.

    In our cell culture systems we mimic disease related lesions for Alzheimer’s Disease, Parkinson’s Disease, Huntington’s Disease, Ischemia and other neuro-degenerative events. In addition, we perform assays for drug safety and have several systems available to test secretase modulators and inhibitors.



    Send us a request to develop a straightforward strategy to support your development program!

    Neurodegenerative diseases Neurodegeneration Neuroinflammation Alzheimers Disease Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Neuroblastoma Primary Cells Primary Neurons Microglia Mouse Embryonic Fibroblasts (MEFs) Cell lines Cell Culture Supernate Astrocytes Mouse Rat Chicken Human CNS/Neurology iPSC Induced Pluripotent Stem Cells Show 21 more tags Show less

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    Primary Cell Culture

    Price on request

    QPS Austria offers functional screening of test compounds in a variety of cell culture models including primary neurons from different species. We provide custom tailored cell culture

    Primary Rat and Mouse Neurons

    • Hippocampal neurons
    • Cortical neurons
    • Dopaminergic neurons from the ventral mesencephalon

    Postnatal Mouse neurons

    • Cerebellar granule cells

    Rat and Murine Glial Cultures

    • Astrocytes
    • Microglia

    Embryonic Chicken Neurons

    • Mesencephalic neurons
    • Telencephalic neurons

    Neuroinflammation Neurodegenerative diseases Primary Neurons Astrocytes Microglia Mouse Embryonic Fibroblasts (MEFs) Mouse Rat Chicken CNS/Neurology Cerebellar granule cells Show 11 more tags Show less

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    Induced Pluripotent Stem Cell (iPSC) Culture

    Price on request

    QPS Neuropharmacology offers functional screening of test compounds in a variety of cell culture models including Induced Pluripotent Stem Cells (iPSC).


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    Cell Lines

    Price on request

    QPS Neuropharmacology offers functional screening of test compounds in a variety of cell lines. We provide custom tailored cell culture systems and would be happy to establish or generate novel transgenic cell lines for you.

     

    • Human or rat neuroblastoma cell lines   
    • Human fibroblasts from patients   
    • Transgenic cell lines overexpressing APP, TAU or TDP43  
    • and many more

    Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Alzheimers Disease CNS/Neurology Cell culture immortalized cell lines Cell Line Characterization Cell Line testing cell line generation Generating Cell Lines Cell-based assays Cell line engineering Cell Proliferation Assays Cell Viability Assays Cellular Compound Screening Stable Cell Line Human Mouse Rat Show 19 more tags Show less

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    Biochemistry & Molecular Biology

    Price on request

    QPS Neuropharmacology’s department of Translational Medicine offers a selection of molecular biological methods for the investigation of various diseases. The unit supports in vitro and in vivo studies performed at QPS Neuropharmacology. However with the same techniques routine sample analyses from external preclinical or clinical studies is performed. Using the same methods for cell culture, animal and human samples brings your compound faster forward.

    Besides standard techniques for protein quantification and enzyme activity assessment, with colorimetric, fluorescence or luminescence detection, like:
       

    • Western blotting     
    • ELISA  
    • Enzymatic assays (e.g. Monoamine Oxidase, Acetyl/Butylcholinesterase activity)


    We also offer protein analysis with all kits from the MSD platform (Mesoscale Discovery). By using this multiplex electrochemiluminescence immunosorbent assay technique, low sample volumes can be used to obtain several readouts within one measurement.

    If you are interested in gene expression and mRNA analysis, we can also investigate your samples with:
       

    • Quantitative real-time PCR/gene expression analysis for any gene of interest     
    • Standard PCR for any gene of interest


    Furthermore, customized assays can be set up and validated, cloning and transfection of cell lines can be performed.

    western blot Western blotting RNA Extraction mRNA ELISA ELISA Protein Analysis protein phosphorylation Protein Profiling Protein Expression Protein Services PCR analysis PCR/Taqman genotyping qRT-PCR Meso Scale Discovery MSD Protein Aggregation cell culture supernatant tissue lysates Plasma Serum CSF Blood biomarker Biochemical Assays Magnetic Activated Cell Sorting (MACS) Show 25 more tags Show less

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    Immunohistochemistry (IHC)

    Price on request

    Staining Techniques and Applications

    We routinely perform both fluorescent and chromogenic immunostainings. Additionally, we are happy to establish and optimize customized staining protocols for your specific needs.

    Our Expertise Includes:

    • Hundreds of established staining protocols
    • Antibody testing and optimization
    • Target validation of viral infections
    • Target validation in transgenic models
    • Compound detection

    Multichannel Immunofluorescence

    • 4-channel immunofluorescence plus DAPI labeling of nuclei
    • Numerous validated antibody combinations for mouse, rat and human tissue

    Dual-stain Immunohistochemistry immunofluorescence labelling Triple-stain Immunohistochemistry antibody validation

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    Special Slide Staining

    Price on request

    We routinely perform both fluorescent and chromogenic immunostainings as well as general histological stainings and stainings for special applications. Additionally, we are happy to establish and optimize customized staining protocols for your specific needs. Our Expertise Includes:

    • Hundreds of established staining protocols
    • Antibody testing and optimization
    • Target validation of viral infections
    • Target validation in transgenic models
    • Compound detection

    Multichannel Immunofluorescence

    • 3-channel immunofluorescence plus DAPI labeling of nuclei
    • Numerous validated antibody combinations for mouse, rat and human tissue

    General stainings

    • Hematoxylin & Eosin (H&E) Staining
    • Cresyl Violet Staining
    • Crystal Violet Staining
    • Luxol Fast Blue Staining
    • Prussian Blue Staining
    • Periodic Acid-Schiff (PAS) Staining
    • Jones' Staining

    Protein Aggregation

    • Thioflavin S Staining
    • Campbell Switzer Staining
    • Gallya’s Staining
    • Congo Red Staining

    Lipid Staining

    • Congo Red Staining
    • BODIPY 493/503

    Silver stainings

    • Campbell Switzer Staining
    • Gallya’s Staining
    • Golgi Cox Staining

    Fluorescence stainings

    • Fluoro Jade C
    • BODIPY 493/503 (Fluoreszenz)
    • Thioflavin S Staining

    Cyclus Analysis

    • Shorr Staining

     

     


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    Animal Learning, Memory, and Behavior Tests

    Price on request

    QPS Neuropharmacology provides a wide range of cognitive tests. Most tests are evaluated using rater-independent digital animal tracking systems.

    • Barnes Maze Test
    • Contextual Fear Conditioning Test
    • Morris Water Maze Test
    • Novel Object Recognition Test
    • Passive and Active Avoidance Test
    • Two Choice Swim Test
    • Y-Maze Test

    Can’t find what you are looking for? We are constantly establishing new tests and would be glad to develop tests according to your requirements.

    Alzheimers Disease Dementia cognition Morris water maze Barnes maze Novel object recognition Contextual Fear Conditioning passive avoidance Two Choice Swim Test Y-Maze Rat Mice Show 12 more tags Show less

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    Object Recognition Test

    Price on request

    This two trial test is used to assess memory for interactions with novel objects. Rodents tend to spend more time interacting with a new object rather than with an already known object. Acquisition: after habituation to the test chamber on the first day, the animal is placed in the box and allowed to explore two identical objects for a set period of time on the second day. Retention: on the third day, the animal is returned to the box where one of the familiar objects has been replaced with a novel object. The time exploring the novel object (relative to the time spent exploring the familiar object) is used as a measure of memory.


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    Morris Water Maze Assay

    Price on request

    The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water, and have to swim to a hidden escape platform. Each mouse has to perform three trials on each of four consecutive days. After the last trial on day 4, mice have to fulfill a probe trial. During the probe trial, the platform is removed from the pool and the number of crossings over the former target position is recorded together with the abidance in this quadrant.


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    Barnes Maze Test

    Price on request

    The Barnes maze is a standard behavioral tool to investigate spatial learning and cognition in rodents.

    The test consists of a circular arena with equally distributed holes on its periphery with one hole serving as target zone that is equipped with the animal’s home cage. The animal can use proximal or distal visual cues to locate and remember the target zone. Automated recordings of consecutive trials provide details on the rodent’s navigation in the arena and thus delivers information about their learning and memory performance.


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    Animal Fear Conditioning Studies

    Price on request

    This test allows the simultaneous assessment of associative learning by simple cues and/or learning by complex stimuli such as context.

    Animals are trained and tested on 2 consecutive days. On the training day animals are placed in a chamber and receive a conditioned stimuli / unconditioned stimuli package (tone/foot shock). Contextual memory is tested 24 hours after training. To do so the animal is placed into the same chamber as during the training and freezing behavior is automatically recorded. Cued memory is scored one hour after the contextual test in a novel, modified chamber where the animal receives the auditory cued stimulus and freezing behavior is again automatically recorded.

    The time spent freezing during the contextual test and tone-dependent test are defined as index of fear-related learning and memory. Cued and contextual fear conditioning is a task that measures the ability of the animal to learn and remember an association between an aversive experience and environmental cues. All parameters of the test are measured automatically with a tracking system.

    Neurodegenerative diseases cognition behavioral assay

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    Animal Motor Function and Activity Testing

    Price on request

    Several customized behavioral tests are offered to phenotype mouse and rat models and evaluate effects of compounds in different in vivo models. New behavioral tests are continuously developed and validated. 

    • Beam Walk and Challenging Beam Walk Test
    • Grip Strength
    • Pasta Gnawing Test
    • Pole Test
    • RotaRod Test
    • Wire Hanging Test

    Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Huntington's Disease rotarod Beam walk Grip strength Wire hanging test Pole test Movement Disorder Show 9 more tags Show less

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    Rotarod Performance Test

    Price on request

    The Rota Rod apparatus is used to measure motor coordination and balance. The time the animal is able to stay on an accelerating rod is measured automatically.

    Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Niemann-Pick Movement Disorder

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    Animal Anxiety and Depression Tests

    Price on request

    Several customized behavioral tests are offered to phenotype mouse and rat models and evaluate effects of compounds in different in vivo models. 

    • Elevated Plus Maze
    • Forced Swim Test
    • Marble Burying Test
    • Novelty-Induced Hypophagia (NIH) Test

    Can’t find what you are looking for? We are constantly establishing new behavioral tests and would be glad to develop new tests according to your requirements.

    psychiatric disorders Schizophrenia Autism Anxiety Depression Novelty-Induced Hypophagia (NIH) Test Marble burying test Elevated plus maze Forced swim test Show 9 more tags Show less

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    Forced Swim Test

    Price on request

    The Forced Swim Test is based on the assumption that animals try to escape from an aversive stimulus. The test is used to measure the effect of antidepressant drugs or the genotype on the emotional status of animals.

    Animals are placed in a transparent Plexiglas cylinder filled with water during test session. The animal’s behavior is recorded and the duration of immobility as well as struggling and swimming is measured. Increased immobility (floating) is interpreted as a behavioral correlate of negative mood, representing a kind of depression in the animal.


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    Elevated Plus Maze Animal Anxiety Test

    Price on request

    The Elevated Plus Maze (EPM) is currently one of the most frequenty used tests to study anxiety in small rodents.

    The test is based on the aversion of rodents to open spaces and height. The EPM apparatus is elevated from the floor. The equipment is a four arm maze with two opposing open arms (lighted arms, without walls) and two opposing closed arms (sheltered arms, with walls). On the test day, the animal is placed in the center area facing the open arm. The behavior during the test session is recorded, and behavioral parameters such as time spent in the open and in the closed arms, number of visits in the open and closed arms as well as latency to enter the open arm are calculated automatically.


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    Animal Social Behavior Test Panel

    Price on request

    Several customized behavioral tests are offered to phenotype mouse and rat models and evaluate effects of compounds in different in vivo models. Most tests are evaluated using rater-independent digital animal tracking systems.
       

    • Three Chamber Social Test
    • Autogrooming Test   
    • The Reeperbahn Test

    Can’t find what you are looking for? We are constantly establishing new tests and would be glad to develop tests according to your requirements!

    Autism Depression Anxiety psychiatric disorders Autogrooming

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    In vitro Neurodegeneration Models

    Price on request

    QPS Neuropharmacology offers functional screening of test compounds in a variety of cell culture models including primary neurons from different species as well as cell lines.

    • Primary Cells   
    • Cell Lines  
    • Transgenic Cell Lines
    • Organotypic Brain Slices
    • iPSCs

    We provide custom tailored cell culture systems and would be happy to establish new primary cell culture systems or generate novel transgenic cell lines for you.

    Stable Cell Line immortalized cell lines microglia Cell culture Primary Neurons Primary Astrocytes Mouse Embryonic Fibroblasts (MEFs) Neurodegeneration Alzheimers Disease Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Neuroinflammation iPSC Show 14 more tags Show less

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    Cell Line Generation

    Price on request

    We provide custom tailored cell culture systems and would be happy to establish new primary cell culture systems or generate novel transgenic cell lines for you.

    immortalized cell lines stable transfection silencing

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    In vitro Excitotoxicity Models

    Price on request

    Excitotoxicity is the pathological process by which neurons are damaged and killed by excessive stimulation by neurotransmitters such as glutamate. QPS Neuropharmacology offers several cellular models comprising primary neurons or transgenic and non-transgenic cell lines to mimic disease related conditions. Cellular models offer the advantage of a controlled environment useful for exploring single pathogenic mechanisms and the proteins involved. Our models are fast and cost-efficient. Customized models are generated gladly on request.


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    Cell Line Characterization

    Price on request
    Request a quote for more information about this service.

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    Mouse Embryonic Fibroblast Generation

    Price on request

    Embryonic primary cells from transgenic mice or rats can be used depending on the prenatal activity of the promoter that controls expression of the transgene.

    Gaucher Huntington's Disease Primary Cells

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    Neurogenesis

    Price on request

    The balance between neurogenesis and cell death plays a critical role not only in the embryonic brain development but also in the maintenance of the adult brain. Alterations in these processes are seen in many neurodegenerative diseases. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to damage at early stages of Alzheimer’s disease (AD). Emerging evidence has indicated that compromised neurogenesis in the adult hippocampus represents an early critical event in the course of AD. For this purpose, QPS Austria provides a cell culture model to assess effects of your developmental compound on neurogenesis of hippocampal neurons.


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    Synaptogenesis Studies

    Price on request

    Synapse formation but especially the synaptic maintenance (synaptogenesis) is critical for neuronal health and disease. Loss of synaptic maintenance is not only restricted to AD. Defects in synaptic maintenance most likely also contribute to the synaptic vulnerability in other neurodegenerative disorders as Huntington’s, Parkinson’s or Niemann Pick disease. A therapeutic strategy that favors synaptic maintenance may be a beneficial target for many neurodegenerative diseases. For this purpose, QPS Neuropharmacology provides a cell culture model to assess effects of your developmental compound on synaptogenesis primary hippocampal neurons.


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    Functional & Cell Type Specific Assays

    Price on request

    Cell lines and primary neurons can be exposed to different toxins and examined for mitochondrial activity, oxidatixe stress, cell viability, apoptosis and lipid metabolism.

    • TMRM and Mitotracker assay for mitochondrial activity
    • YO-PRO-1 Iodide for apoptosis
    • Colorimetric assays such as TBARS assay for oxidative stress

    Can’t find what you are looking for? We are constantly establishing new methods and would be glad to develop new assays according to your requirements.

    Mitochondrial assay cellular pathway Pathway analysis

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    Cytotoxicity Assays

    Price on request

    Cell lines and primary neurons can be exposed to different toxins and examined for cell viability and apoptosis.

    • LDH assay
    • MTT assay
    • YO-PRO™-1 Iodide assay
    • Caspase assay
    • Propidium Iodide (PI) assay

    Can’t find what you are looking for? We are constantly establishing new methods and would be glad to develop assays according to your requirements.

    In Vitro Viability And Toxicity Assay Cell Viability Assays viability testing Apoptosis assay MTT And MTS Assays caspase Show 6 more tags Show less

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    Mitochondrial Toxicity Studies

    Price on request

    A reduction in mitochondrial activity has been strongly linked with various neurodegenerative diseases. Moreover, several mutations have been characterized that induce age-related mitochondrial malfunction. QPS Austria provides cell culture models that address the effect of compounds on mitochondrial activity and mitochondria related cell death in vitro.

    Cell lines and primary neurons can be exposed to different toxins and examined for mitochondrial activity and mitochondrial membrane depolarization.

    • Mitochondrial Activity (MitoTracker)
    • TMRM assay


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    Animal Model Housing and Maintenance

    Price on request

    As a leading CRO for CNS drug development, QPS Neuropharmacology is the premier provider for services with transgenic animals. The enterprise gained experience in generating, characterizing and maintaining transgenic disease models and using them for drug testing projects for more than 15 years.

    The fully AAALAC accredited Animal Facility provides all in vivo services from mouse and rat breeding to delivering study reports that contain all experimental procedures and their outcome. Animals are housed under SPF conditions in IVC systems with periodic health monitoring. Our approach is based on a sequence of procedural building blocks that allow customization of any service request according to your specific needs. You have the option to select any entry point and exit point in our workflow.

    Alzheimers Disease Amyotrophic Lateral Sclerosis (ALS) Parkinson's disease (PD) Orphan Diseases Lysosomal Storage Disorders

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    Rodent Breeding

    Price on request

    Just discuss your needs with us, and we take care of your mice and rat breeding needs. Ship your own rodent model to us or decide for one of our life stock rodent models. Upon request we breed as many rodents as needed for your experiments. Once breeding is completed animals can be aged in house as long as demanded. Afterwards, animals can either be shipped to you or can be used in-house for studies according to your study needs.

    Animal breeding and maintenance is performed by our trained animal keepers. Health monitoring is continuously performed by our in-house veterinarian.


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    Animal Surgery

    Price on request

    Our expertise covers a wide range of surgery techniques in mice and rats that include the following (but are not limited to them):

    • Implantation of osmotic pumps
    • Catheterization
    • Stereotactic injections into hippocampus, cortex, ventricle, striatum or almost any other brain region to inject:
      • Drugs
      • Test items
      • Toxins
      • Viruses (Biosafety level 2)

    Our expertise covers a wide range of treatment techniques in mice and rats that include the following routes but are not limited to them:

    Treatment routes

    Intraperitoneal
    Intravenous
    Subcutan
    Intranasal
    Oral (Gavage, via food pellets or drinking water)
    Anesthesia / Surgeries

    Implantation of osmotic pumps
    Stereotactic injections into hippocampus, cortex, ventricle, striatum or almost any other brain region to inject:Drugs
    Test items
    Toxins
    Viruses (S2)

    catheter catheterizations Stereotactic Injections osmotic minipump lentivirus adenovirus Mouse Rat Show 8 more tags Show less

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    Animal Blood Collection

    Price on request

    We routinely perform in vivo blood sampling in mice and rats.

    • Vena mandibularis bleeding
    • Tail vein bleeding
    • Heart puncture (terminal)
    • Tail vein bleeding for immediate glucose measurements

    Can’t find what you are looking for? We are constantly establishing new methods and would be glad to develop new methods according to your requirements.

    Blood Mouse Rat

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    Transgenic Mouse Services

    Price on request

    QPS Neuropharmacology is experienced in generating, characterizing and maintaining of transgenic disease models and using them for drug testing projects for more than 15 years. 

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Gaucher Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.

    We are happy to receive any inquiry to elaborate the most efficient way, including behavioral, immunohistochemical, biochemical and molecular biological techniques, for the support of your drug development approach.

    Our biobank can provide samples (brain tissue, CSF, etc.) of our animal models for analyses in your laboratory.

    Orphan Diseases Rare Disease Lysosomal Storage Disorders Neurodegenerative diseases Amyotrophic Lateral Sclerosis (ALS) Alzheimers Disease Niemann-Pick Gaucher Mucopolysaccharidosis Rat Mouse Show 11 more tags Show less

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    Genotyping and Gene Expression Assays

    Price on request
    Request a quote for more information about this service.

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    Mouse Phenotyping

    Price on request

    Several customized behavioral tests are offered to phenotype mouse and rat models and evaluate effects of compounds in different in vivo models. 

    New behavioral tests are continuously developed and validated.


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    Cognitive, Behavioral, and Psychiatric Animal Models

    Price on request

    QPS Neuropharmacology is experienced in generating, characterizing and maintaining of transgenic disease models and using them for drug testing projects for more than 15 years.

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Gaucher Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.

    We are happy to receive any inquiry to elaborate the most efficient way, including behavioral, immunohistochemical, biochemical and molecular biological techniques, for the support of your drug development approach.

    Neurodegenerative diseases Neurodegeneration psychatric disorders Schizophrenia Autism mental disorders Dementia Rare Disease Alzheimers Disease Parkinson's disease (PD) CNS/Neurology Induced-disease models Mice Rat Psychiatry/Mental Health Show 15 more tags Show less

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    Neurodegeneration Animal Models

    Price on request

    QPS Neuropharmacology is experienced in generating, characterizing and maintaining of transgenic disease models and using them for drug testing projects for more than 15 years.

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Lobar Degeneration (FTLD), allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.


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    Cognition and Behavior Animal Models

    Price on request

    QPS Neuropharmacology is experienced in generating, characterizing and maintaining of transgenic disease models and using them for drug testing projects for more than 15 years.

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Frontotemporal Lobar Degeneration (FTLD) and other neurodegenerative diseases allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.

    We are happy to receive any inquiry to elaborate the most efficient way, including behavioral, immunohistochemical, biochemical and molecular biological techniques, for the support of your drug development approach.

    cognition Dementia Frontotemporal Lobar Degeneration Alzheimers Disease Morris water maze Open field Y-Maze Two Choice Swim Test passive avoidance Novel object recognition Contextual Fear Conditioning Amyloid TAU Neuroinflammation Show 14 more tags Show less

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    Lesion-Induced CNS Animal Models

    Price on request

    QPS Neuropharmacology offers lesion induced animal models for drug efficacy testing.
       

    • MPTP Mouse Model for Parkinson's Diseases
    • 6-OHDA Lesion Model for Parkinson's Diseases


    Can’t find what you are looking for? We are constantly establishing new CNS animal models and would be glad to develop an animal model according to your requirements.

    Parkinson's disease (PD) 6-OHDA MPTP Lesion model MPTP- induced parkinson model L-DOPA

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    LPS-Induced Inflammation Animal Models

    Lipopolysaccharide-Induced Inflammation Animal Models
    Price on request

    Neuroinflammation is a common feature of different neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s disease, Frontotemporal dementia, Amyotrophic lateral sclerosis and several others. Recent research suggests that targeting neuroinflammation might present a valid method to treat neurodegenerative diseases. In order to model neuroinflammation independent from other disease-relevant pathologies, wildtype mice can be peripherally injected with lipopolysaccharide (LPS).

    Stereotactic Injections intracerebral Intraperitoneal Neuroinflammation

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    Multiple Sclerosis Animal Models

    Price on request

    The cuprizone mouse model captures several aspects of MS pathology like demyelination / remyelination, cognitive decline, altered activity and motor deficits. The cuprizone model is the most frequently used model among the toxin-induced MS models, which include lysolecithin and ethidium bromide, and is used to study mechanisms of oligodendrocyte turnover, gliosis as well as motor capabilities. This animal model is thus be suitable to assess certain aspects of MS pathology and to test pharmaceutical compounds.

    Multiple sclerosis (MS) cuprizone Myelin Basic Protein myelination MOG- induced Experimental Autoimmune Encephalomyelitis (EAE) cell trafficking CNS inflammation Autoimmunity Demyelination Show 9 more tags Show less

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    Tissue Embedding

    Price on request

    Just discuss your needs with us, and we take care of dissecting the target tissue, accurate trimming of the tissue block, fixation and embedding of your tissue samples. Highly standardized default procedures are established, but alternatives can be used upon request. We are experienced working RNase-free.

    Fixation

    • Standard: 4% paraformaldehyde
    • Alternatives: Glutaraldehyde, zinc, picric acid
    • Buffers: PBS, cacodylate, sulfide

    Embedding

    • Standard: OCT or paraffin (Sakura, Tissue-Tek TEC)
    • Alternative: Plastic


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    Paraffin Embedding

    Price on request

    Just discuss your needs with us, and we take care of dissecting the target tissue, accurate trimming of the tissue block, fixation and embedding of your tissue samples. Highly standardized default procedures are established, but alternatives can be used upon request. We are experienced working RNase-free.

    Fixation

    • Standard: 4% paraformaldehyde
    • Alternatives: Glutaraldehyde, zinc, picric acid
    • Buffers: PBS, cacodylate, sulfide

    Embedding

    • Standard: OCT or paraffin (Sakura, Tissue-Tek TEC)
    • Alternative: Plastic


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    Tissue Sectioning

    Price on request

    Our expertise covers a wide range of sectioning techniques and tissue collection strategies. We process your samples according to the specific requirements of your project.

    Sectioning

    • Sliding microtome (Leica, SM2000R)
    • Cryotome (Leica, CM1950S and CM3050S)
    • Vibrating blade microtome (Leica, VT1000S)
    • Choose any orientation and specify the section thickness

    Collecting

    • Specify your collection scheme
    • Systematic uniform random sampling available
      (For explanation see: http://www.stereology.info/sampling/)
    • Free floating sections
    • Sections mounted on slides
    • Choose slides according to your needs


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    Immunofluorescence

    Price on request

    We routinely perform both fluorescent immunostainings as well as general histological stainings. We have established protocols for more than hundred stainings.

    Additionally, we are happy to establish and optimize customized staining protocols for your specific needs. Our expertise also includes

    • Antibody testing and optimization
    • Target validation of viral infections
    • Target validation in transgenic models
    • Compound detection

    Examples for established staining protocols:

    • Neuronal markers (beta-III tubulin, neurofilament, MAP2, tyrosine hydroxylase)‏ 
    • Glial markers (GFAP, CD11b)‏ 
    • Disease related markers (Tau, phosphorylated Tau, signaling molecules, synuclein)‏ -
    • Synaptic proteins (synaptophysin)‏

    CNS/Neurology histology Histology assessment immunofluorescence labelling immunohistochemistry Triple-stain Immunohistochemistry antibody validation Fluorescence in situ Hybridization Show 8 more tags Show less

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    H&E Staining

    Hematoxylin and eosin staining
    Price on request

    We routinely perform both fluorescent and chromogenic immunostainings as well as general histological stainings and stainings for special applications.

    Additionally, we are happy to establish and optimize customized staining protocols for your specific needs.


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    Image Analysis

    Price on request

    We use sophisticated macro-based software solutions to automatically analyze different features of brain morphology and pathology, e.g. numerical densities of cell somata or axonal fibers, synaptic density, dendritic arborization, area and volume of brain areas or plaques. We are pleased to develop new quantification methods, as well as to assess used (feasibility analysis) or ameliorate existing methods (proof of measurement).

    Identification and Detection of Labeled Structures

    • Accuracy of detected labeled structures through:
      • Validation of antibodies on knockout tissue whenever possible
      • Appropriate imaging controls
    • Determination and consistent usage of thresholding and filtering parameters throughout the whole study

    Analysis of Region and Object Features

    We routinely offer a wide variety of automatically generated outcome measures. These include:

    • Total immuno-reactive area
    • Sum or mean fluorescence intensity of immuno-reactive particles
    • Number, size, density and shape of labeled particles
    • Size exclusions and binning of objects
    • Exact morphometric delineation of regions of interest for volume estimation
    • And many more


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    Biology

    Price on request

    QPS Neuropharmacology’s department of Translational Medicine offers a selection of molecular biological methods for the investigation of various diseases. The unit supports in vitro and in vivo studies performed at QPS Neuropharmacology. However with the same techniques routine sample analyses from external preclinical or clinical studies is performed. Using the same methods for cell culture, animal and human samples brings your compound faster forward.

    Protein Services western blot ELISA qRT-PCR RNA Extraction Protein Aggregation protein phosphorylation protein quantification DNA Isolation Meso Scale Discovery PCR Cell-based assays Amyloid TAU SOD1 TDP43 Cytokines Show 17 more tags Show less

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    Tissue Processing

    Price on request

    Tissue samples are routinely prepared and fractionized according to established methods.

    • Brain tissue e.g. processed to soluble or insoluble fractions
    • Extraction and purification of DNA/RNA/protein


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    Protein Services

    Price on request

    Besides standard techniques for protein quantification and enzyme activity assessment, with colorimetric, fluorescence or luminescence detection, like:

    • Western blotting
    • ELISA
    • Enzymatic assays (e.g. Monoamine Oxidase, Acetyl/Butylcholinesterase activity

    We also offer protein analysis with all kits from the MSD platform (Mesoscale Discovery). By using this multiplex electrochemiluminescence immunosorbent assay technique, low sample volumes can be used to obtain several readouts within one measurement.

    • Inflammation marker/cytokines (TNF-a, IFN-γ, IL,….)
    • Abeta 38, 40, 42
    • (p-)Tau,
    • Synuclein
    • and many more

    Additionally also the areas of oxidative stress and lipid metabolism can be investigated in house:

    • Colorimetric assays such as TBARS assay for oxidative stress
    • Assessment of total cholesterol levels in plasma and tissues

    Can’t find what you are looking for? We are constantly establishing new methods and would be glad to develop assays according to your requirements.

    ELISA quantitative western blot Western blotting Meso Scale Discovery Blood Plasma CSF Frozen Tissue Tissue Tissue Homogenate Rodent Tissue Show 11 more tags Show less

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    Protein Quantification

    Price on request
    Request a quote for more information about this service.

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    Western Blot

    Price on request
    • Synaptic proteins (glutamate receptor 1, NMDA receptors, synaptophysin, drebrin) e.g. from matured hippocampal neurons
    • Tau and phospho-Tau isoforms
    • Abeta peptide profile, Abeta aggregation assays
    • Cell signaling molecules
    • TDP43
    • Synuclein


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    ELISA

    Enzyme-linked immunosorbent assay
    Price on request
    • Abeta1-38, Abeta1-40, Abeta1-42, sAPP-alpha, sAPP-beta
    • Tau and phospho Tau profile
    • Signal transduction molecules
    • Inflammation markers (e.g. Prostaglandin E2)
    • Cytokine Profile


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    qRT-PCR

    Quantitative reverse transcription polymerase chain reaction
    Price on request

    If you are interested in gene expression and mRNA analysis, we can investigate your samples with:

    • Quantitative real-time PCR/gene expression analysis for any gene of interest
    • Standard PCR for any gene of interest

    Furthermore, customized assays can be set up and validated.

    Quantitative Polymerase Chain Reaction RT-PCR Real time qPCR mRNA

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    LC-MS/MS

    Liquid chromatography tandem mass spectrometry
    Price on request
    Request a quote for more information about this service.

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    Statistical Analysis

    Price on request

    Statistical Data Analysis

    • Professional statistical data analysis (unbiased and representative) performed by our team of scientists and biostatisticians   
    • Conscientious quality checks of measurements and results

    Reporting of Experiments and Results

    • At the end of each study our customers receive a full study report including all experimental procedures, qualitative and quantitative assessment of the results, as well a comprehensive statistical analysis. Constant updates throughout the study are provided if desired.

    Data analysis Data analysis workflow Data Report Biostatistics

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    Cells and Tissues

    Price on request

    QPS Neuropharmacology provides in vitro research services with numerous standardized cell culture systems including transgenic and non-transgenic cell lines, glial cells, primary chicken and rat peripheral and central nervous system neurons of different developmental stages and organotypic brain slices. New models are developed and validated on request.

    QPS Neuropharmacology’s department of Translational Medicine offers a selection of molecular biological methods for the investigation of various diseases. The unit supports in vitro and in vivo studies performed at QPS Neuropharmacology. However with the same techniques routine sample analyses from external preclinical or clinical studies is performed. Using the same methods for cell culture, animal and human samples brings your compound faster forward.


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    Genetic Engineering

    Price on request

    We provide custom tailored cell culture systems and would be happy to generate novel transgenic cell lines for you.

    We are experienced in performing in vitro and in vivo assays using lentivirus (LV) or adeno-associated virus (AAV).


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    In vitro Neurotoxicity Testing

    Price on request

    QPS Neuropharmacology provides in vitro research services with numerous standardized cell culture systems including transgenic and non-transgenic cell lines, glial cells, primary chicken and rat peripheral and central nervous system neurons of different developmental stages and organotypic brain slices. New models are developed and validated on request.

    In our cell culture systems we mimic disease related lesions for Alzheimer’s Disease, Parkinson’s Disease, Huntington’s Disease, Ischemia and other neuro-degenerative events. In addition, we perform assays for drug safety and have several systems available to test secretase modulators and inhibitors.

    Neurodegenerative diseases Ischemia CNS/Neurology Oxidative Stress Neuroinflammation

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    Cell-Based Assays

    Price on request

    Cell lines and primary neurons can be exposed to different toxins and examined for mitochondrial activity, oxidatixe stress, cell viability and apoptosis.
       

    • TMRM and Mitotracker assay for mitochondrial activity
    • YO-PRO-1 Iodide for apoptosis  
    • Colorimetric assays such as TBARS assay for oxidative stress


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    Mitochondrial Stress Tests

    Price on request

    A reduction in mitochondrial activity has been strongly linked with various neurodegenerative diseases. Moreover, several mutations have been characterized that induce age-related mitochondrial malfunction. QPS Neuropharmacology provides cell culture models that address the effect of compounds on mitochondrial activity and mitochondria related cell death in vitro.

    Cell lines and primary neurons can be exposed to different toxins and examined for mitochondrial activity and mitochondrial membrane depolarization.

    • Mitochondrial Activity (MitoTracker)  
    • TMRM assay

    Mitochondrial assay CNS/Neurology

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    Apoptosis Assays

    Price on request

    Cell lines and primary neurons can be exposed to different toxins and examined for apoptosis.

    • YO-PRO™-1 Iodide assay
    • Caspase assay

    Can’t find what you are looking for? We are constantly establishing new methods and would be glad to develop assays according to your requirements.


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    Necrosis Assays

    Price on request

    Cell lines and primary neurons can be exposed to different toxins and examined for necrosis.

    • LDH assay
    • Propidium Iodide (PI) assay

    Can’t find what you are looking for? We are constantly establishing new methods and would be glad to develop assays according to your requirements.


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    LDH Release Assay

    Lactate Dehydrogenase Release Assay
    Price on request

    Cell lines and primary neurons can be exposed to different toxins and examined for cytotoxicity using the LDH assay. Lactate dehydrogenase (LDH) is a stable cytoplasmatic enzyme present in all cells. It is rapidly releasedinto the cell culture supernatant upon damage of the plasma membrane.


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    MTT and MTS Assays

    Price on request

    Cell lines and primary neurons can be exposed to different toxins and examined for cell viability using the MTT assay. The MTT-assay is based on the metabolicreduction of MTT, a substance with yellow color, to a blue formazan product by mitochondrialdehydrogenases. Only viable cells are able to catalyze this reaction.

     


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    Animal Models of Disease

    Price on request

    QPS Neuropharmacology is experienced in generating, characterizing and maintaining of transgenic disease models and using them for drug testing projects for more than 15 years. 

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Gaucher Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.

    We are happy to receive any inquiry to elaborate the most efficient way, including behavioral, immunohistochemical, biochemical and molecular biological techniques, for the support of your drug development approach.

    Our biobank can provide samples (brain tissue, CSF, etc.) of our animal models for analyses in your laboratory.

    Neurodegeneration cognition Dementia Alzheimers Disease Parkinson's disease (PD) psychiatric disorders Amyotrophic Lateral Sclerosis (ALS) Niemann-Pick Lysosomal Storage Disorders Gaucher Alport Mucopolysaccharidosis Huntington's Disease Multiple sclerosis (MS) Schizophrenia Autism Depression Neuroscience Movement Disorder Show 19 more tags Show less

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    Rodent Models

    Price on request
    Request a quote for more information about this service.

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    Rat Models

    Price on request
    Request a quote for more information about this service.

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    Mouse Models

    Price on request
    Request a quote for more information about this service.

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    Animal Model in vivo Analyses

    Price on request

    QPS Neuropharmacology provides a wide range of behavioral tests for various indications. Most tests are evaluated using rater-independent digital animal tracking systems.

    • General Health & Activity Levels
    • Cognitive Tests
    • Motor Ability Tests
    • Anxiety & Depression Tests
    • Social Behavior Tests
    • Lateral Lesion Tests

    Can’t find what you are looking for? We are constantly establishing new tests and would be glad to develop tests according to your requirements.

    Animal Movement Analysis animal monitoring transgenic animal model cognition and memory health Monitoring survival analysis Show 6 more tags Show less

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    Animal Cognition & Behavior Tests

    Price on request

    QPS Neuropharmacology provides a wide range of cognitive tests. Most tests are evaluated using rater-independent digital animal tracking systems.

    • Barnes Maze Test
    • Contextual Fear Conditioning Test
    • Morris Water Maze Test
    • Novel Object Recognition Test
    • Passive and Active Avoidance Test
    • Two Choice Swim Test
    • Y-Maze Test

    Can’t find what you are looking for? We are constantly establishing new tests and would be glad to develop tests according to your requirements.

    Alzheimers Disease Dementia cognition Neurodegeneration Neurodegenerative diseases Morris water maze Contextual Fear Conditioning Y-Maze Two Choice Swim Test passive avoidance Novel object recognition Mouse Rat Mice rats Show 15 more tags Show less

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    Animal Motor Function Tests

    Price on request

    Several customized behavioral tests are offered to phenotype mouse and rat models and evaluate effects of compounds in different in vivo models. New behavioral tests are continuously developed and validated. 

    • Beam Walk and Challenging Beam Walk Test
    • Grip Strength
    • Pasta Gnawing Test
    • Pole Test
    • RotaRod Test
    • Wire Hanging Test

    Huntington's Disease Parkinson's disease (PD) Amyotrophic Lateral Sclerosis (ALS) Niemann-Pick rotarod Beam walk Grip strength Wire hanging test Pasta Gnawing Movement Disorder Show 10 more tags Show less

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    Animal Social Domain Tests

    Price on request

    QPS Neuropharmacology provides a wide range of  social domain tests for various indications. Most tests are evaluated using rater-independent digital animal tracking systems.

    • Three Chamber Social Test
    • Autogrooming Test
    • The Reeperbahn Test

    Can’t find what you are looking for? We are constantly establishing new tests and would be glad to develop tests according to your requirements!


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    Animal Social Behavior Tests

    Price on request

    QPS Neuropharmacology provides a wide range of  social behavior tests for various indications. Most tests are evaluated using rater-independent digital animal tracking systems.

    • Three Chamber Social Test
    • Autogrooming Test
    • The Reeperbahn Test


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    Histological Staining

    Price on request

    Staining Techniques and Applications

    We routinely perform both fluorescent and chromogenic immunostainings as well as general histological stainings and stainings for special applications. Additionally, we are happy to establish and optimize customized staining protocols for your specific needs.

    Our Expertise Includes:

    • Hundreds of established staining protocols
    • Antibody testing and optimization
    • Target validation of viral infections
    • Target validation in transgenic models
    • Compound detection

    Multichannel Immunofluorescence

    • 4-channel immunofluorescence plus DAPI labeling of nuclei
    • Numerous validated antibody combinations for mouse, rat and human tissue

    General Stainings

    • Hematoxilin-Eosin
    • Cresyl violet (Nissl)
    • Luxol fast blue
    • … and many more

    Special Stainings

    • Thioflavin S or Congo Red (amyloid plaques, tau tangles)
    • Golgi-Cox silver impregnation
    • Prussian Blue (microbleedings)


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    Common Histochemical Stains

    Price on request

    General stainings

    • Hematoxylin & Eosin (H&E) Staining
    • Cresyl Violet Staining
    • Crystal Violet Staining
    • Luxol Fast Blue Staining
    • Prussian Blue Staining
    • Periodic Acid-Schiff (PAS) Staining
    • Jones' Staining


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    Immunostaining

    Price on request

    Staining Techniques and Applications

    We routinely perform both fluorescent and chromogenic immunostainings as well as general histological stainings and stainings for special applications. Additionally, we are happy to establish and optimize customized staining protocols for your specific needs.

    Our Expertise Includes:

    • Hundreds of established staining protocols
    • Antibody testing and optimization
    • Target validation of viral infections
    • Target validation in transgenic models
    • Compound detection


    Multichannel Immunofluorescence

    • 4-channel immunofluorescence plus DAPI labeling of nuclei
    • Numerous validated antibody combinations for mouse, rat and human tissue

     

    immunohistochemistry antibody validation

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    Microscopy

    Price on request

    Multidimensional Image Acquisition

    What we offer:

    • Automated and comprehensive multidimensional image acquisition
    • Mosaic (tiled) high-resolution images for systematic quantifications
    • Up to 5-channel epifluorescence imaging (green, red, infrared, far red, and nuclear dye DAPI)
    • Systematic uniform random sampling available
      (For explanation see: http://www.stereology.info/sampling/)

    Appropriate Imaging Controls

    Autofluorescence is an often times neglected, yet serious problem. Most tissues exhibit a certain level of autofluorescence that might be mistaken as specific staining. The unspecific signal usually increases with age and with the size of neuronal cells; this is therefore a common nuisance in human brain samples.

    To control for this phenomenon, we often devote one of the four available fluorescent channels to image the „intrinsic“  fluorescence. Subtraction of this channel from the „labeled“ channels allows for determination of the specific signal deriving from targeted structures.


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    Protein Expression Visualization

    Price on request
    Request a quote for more information about this service.

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    Toxicology

    Price on request
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    Pharmacology & Toxicology

    Price on request
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    Biomarkers

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    The Expertise of QPS Neuropharmacology lies within the field of CNS Diseases, Orphan Diseases & Mental Disorders. We provide a state of the art research environment (AAALAC certified) for testing and evaluating new potential treatment approaches. Besides offering a broad spectrum of transgenic models and applications, we offer the establishment of new and customized assays that suit your special needs.

    Research Fields

    • Alzheimer's Disease 
    • Parkinson's Disease   
    • Tauopathies
    • Orphan Diseases
    • Lysosomal Storage Diseases   
    • Psychiatric Disease s 
    • Inflammation

    Applications

    • Gene & Protein Expression   
    • Enzyme Activity Assays
    • Customized Cloning  
    • Cytokine Profile 
    • Lipid Profile 
    • A4 Assay

    Neurodegeneration Neurodegenerative diseases Parkinson's disease (PD) Alzheimers Disease Orphan Diseases Rare Disease psychiatric disorders Lysosomal Storage Disorders Huntington's Disease Niemann-Pick Multiple sclerosis (MS) Dementia Neuroinflammation Neurofilament Light Chain (NF-L) Show 14 more tags Show less

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    Biospecimens

    Price on request

    QPS Neuropharmacology’s well characterized and validated in vivo models are useful tools to push your CNS drug discovery research forward. We are happy to support your research activities with sample material from our biobank composed of various specimen derived from our in-house in vivo models. Tissues of transgenic and non-transgenic animals of various age groups are readily available such as brain tissue, CSF and plasma samples.


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    Data Services

    Price on request

    Statistical Data Analysis

    • Professional statistical data analysis (unbiased and representative) performed by our team of scientists and biostatistician  
    • Conscientious quality checks of measurements and results

    Reporting of Experiments and Results

    • At the end of each study our customers receive a full study report including all experimental procedures, qualitative and quantitative assessment of the results, as well a comprehensive statistical analysis. Constant updates throughout the study are provided if desired.

    Biostatistics Data analysis

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    Bioanalysis

    Price on request
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    In vitro Toxicity Testing

    Price on request

    QPS Neuropharmacology provides in vitro research services with numerous standardized cell culture systems including transgenic and non-transgenic cell lines, glial cells, primary chicken and rat peripheral and central nervous system neurons of different developmental stages and organotypic brain slices. New models are developed and validated on request.

    In our cell culture systems we mimic disease related lesions for Alzheimer’s Disease, Parkinson’s Disease, Huntington’s Disease, Ischemia and other neuro-degenerative events. In addition, we perform assays for drug safety and have several systems available to test secretase modulators and inhibitors.


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    Cell Death Assays

    Price on request

    Cell lines and primary neurons can be exposed to different toxins and examined for cell death.

    • YO-PRO™-1 Iodide assay
    • Caspase assay
    • Propidium Iodide (PI) assay

    Can’t find what you are looking for? We are constantly establishing new methods and would be glad to develop assays according to your requirements.

    Apoptosis assay Caspase Assay

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    RT-PCR

    Reverse transcription polymerase chain reaction
    Price on request

    If you are interested in gene expression and mRNA analysis, we can investigate your samples with:

    • Quantitative real-time PCR/gene expression analysis for any gene of interest  
    • Standard PCR for any gene of interest

    Furthermore, customized assays can be set up and validated.

    qRT-PCR Real time qPCR RT-PCR real time PCR mRNA

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    Biospecimen Management and Storage

    Price on request

    QPS Neuropharmacology’s well characterized and validated in vivo models are useful tools to push your CNS drug discovery research forward. We are happy to support your research activities with sample material from our biobank composed of various specimen derived from our in-house in vivo models. Tissues of transgenic and non-transgenic animals of various age groups are readily available such as brain tissue, CSF and plasma samples.


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    Drug Discovery & Development

    Price on request

    As a leading CRO for CNS drug development, QPS Neuropharmacology is the premier provider for services with transgenic animals. The enterprise gained experience in generating, characterizing and maintaining transgenic disease models and using them for drug testing projects for more than 15 years.

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.


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    Drug Development

    Price on request

    As a leading CRO for CNS drug development, QPS Neuropharmacology is the premier provider for services with transgenic animals. The enterprise gained experience in generating, characterizing and maintaining transgenic disease models and using them for drug testing projects for more than 15 years.

    The mouse and rat models available for drug development in Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD), Niemann-Pick Disease, Amyotrophic Lateral Sclerosis (ALS) , Autism Spectrum Disorder (ASD), Schizophrenia, Frontotemporal Lobar Degeneration (FTLD), and other neurodegenerative diseases allow the quantitative evaluation of compound interactions with amyloid, tau, inflammation related markers, mitochondrial deficiency and other pathologies.


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    Clinical Research

    Price on request
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    Clinical Laboratory Services

    Price on request
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    Neuroscience

    Price on request

    QPS Neuropharmacology is the division of QPS that focuses on preclinical studies in CNS diseases, Orphan Diseases and Mental Disorders. The on-site availability of highly predictive disease models and unparalleled experience with studies performed for biopharmaceutical companies of all sizes makes QPS Neuropharmacology the first choice for most CNS drug development needs.

    Validated transgenic and non-transgenic in vitro and in vivo models cover most targets of Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal Dementia (FTD), Niemann-Pick Disease, Autism Spectrum Disorder (ASD), Schizophrenia, Lewy Body Dementia (LBD) and other neurodegenerative diseases.

    Neurodegenerative diseases Alzheimers Disease Parkinson's disease (PD) Niemann-Pick Gaucher Alport Lysosomal Storage Disorders Neuroinflammation Amyotrophic Lateral Sclerosis (ALS) cognition Biochemistry molecular biology histology behavioral assay animal model development CNS/Neurology Show 16 more tags Show less

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    Lysosomal Storage Disease Animal Models

    Price on request

    QPS Neuropharmacology offers mouse models available for drug development in lysosomal storage diseases.

    Niemann-Pick Disease
       

    • NPC1-/- Mouse Model

    Gaucher Disease
       

    • 4L/PS-NA Mouse Model
    • CBE-Induced Mouse Model

    Mucopolysaccharidosis   


    • MPS IIIA Mouse Model


    We are happy to receive any inquiry to elaborate the most efficient way, including behavioral, immunohistochemical, biochemical and molecular biological techniques, for the support of your drug development approach.

    Gaucher Niemann-Pick Mucopolysaccharidosis

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    EA

    Ewald Auer

    Director of Business Development
    CC

    Charlene Chen

    Deputy General Manager & Head of CTPS
    MD

    Magdalena Daurer

    Deputy Head of Histology
    CH

    Christina Hoeller

    Business Development Manager
    JK

    Jan Kehr

    Collaborator
    SK

    Stefanie Kraßnig

    Business Development Manager
    TL

    Tina Löffler

    Head of In-Vitro Research
    ÁM

    Ágnes Molnár-Kasza

    Preclinical Research Associate
    JN

    Joerg Neddens

    Head of Histology
    MP

    Maria Posch

    Preclinical Research Associate
    RR

    Roland Rabl

    Deputy Head of In-Vivo Research
    • Positive review for Cells and Tissues:

      May 2020

      "great service, very fast & flexible. Nice reporting. Thanks for this great service!"

    QPS Neuropharmacology has not received any endorsements.