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QPS Austria GmbH

Grambach, AT

QPS Austria, part of the QPS group, is a full-service contract research organization (CRO). The company performs preclinical as well as clinical research.

QPS Austria Neuropharmacology is well known as a leading CRO for CNS drug development. The availability of the most predictive disease models and unparalleled experience with studies performed for biopharmaceutical companies of all sizes turn QPS into the first choice for most research needs. Validated transgenic and non-transgenic in vitro and in vivo models cover most targets of AD, PD, HD and other neurodegenerative diseases.

The clinical research team ensures an efficient development process from First-in-Man (Phase I) up to and including Post-Marketing-Surveillance-Studies (Phase IV). Our customers benefit from the competent teams at QPS Austria, the use of innovative technology as well as scientific and clinical expertise together with a worldwide... Show more »

QPS Austria, part of the QPS group, is a full-service contract research organization (CRO). The company performs preclinical as well as clinical research.

QPS Austria Neuropharmacology is well known as a leading CRO for CNS drug development. The availability of the most predictive disease models and unparalleled experience with studies performed for biopharmaceutical companies of all sizes turn QPS into the first choice for most research needs. Validated transgenic and non-transgenic in vitro and in vivo models cover most targets of AD, PD, HD and other neurodegenerative diseases.

The clinical research team ensures an efficient development process from First-in-Man (Phase I) up to and including Post-Marketing-Surveillance-Studies (Phase IV). Our customers benefit from the competent teams at QPS Austria, the use of innovative technology as well as scientific and clinical expertise together with a worldwide network.

Recent Publications

  • Late-stage α-synuclein accumulation in TNWT-61 mouse model of Parkinson's disease detected by diffusion kurtosis imaging. Khairnar A, Ruda-Kucerova J, Drazanova E, Szabó N, Latta P, Arab A, Hutter-Paier B, Havas D, Windisch M, Sulcova A, Starcuk Z Jr, Király A, Rektorova I. J Neurochem. 2015 Dec 19. doi: 10.1111/jnc.13500. [Epub ahead of print]
  • Diffusion Kurtosis Imaging Detects Microstructural Alterations in Brain of α-Synuclein Overexpressing Transgenic Mouse Model of Parkinson's Disease: A Pilot Study.
    Khairnar A, Latta P, Drazanova E, Ruda-Kucerova J, Szabó N, Arab A, Hutter-Paier B, Havas D, Windisch M, Sulcova A, Starcuk Z Jr, Rektorova I. Neurotox Res. 2015 Jul 8. [Epub ahead of print]
  • Influence of Lentiviral β-Synuclein Overexpression in the Hippocampus of a Transgenic Mouse Model of Alzheimer's Disease on Amyloid Precursor Protein Metabolism and Pathology.
    Krassnig S, Schweinzer C, Taub N, Havas D, Auer E, Flunkert S, Schreibmayer W, Hutter-Paier B, Windisch M. Neurodegener Dis. 2015 Jun 25.
  • Long-term treatment of aged Long Evans rats with a dietary supplement containing neuroprotective peptides (N-PEP-12) to prevent brain aging: effects of three months daily treatment by oral gavage.
    Hutter-Paier B, Reininger-Gutmann B, Wronski R, Doppler E, Moessler H. J Med Life. 2015 Apr-Jun;8(2):207-12.
  • Multigram Synthesis and in Vivo Efficacy Studies of a Novel Multitarget Anti-Alzheimer's Compound
    Sola I, Viayna E, Gómez T, Galdeano C, Cassina M, Camps P, Romeo M, Diomede L, Salmona M, Franco P, Schaeffer M, Colantuono D, Robin D, Brunner D, Taub N, Hutter-Paier B, Muñoz-Torrero D. Molecules. (March, 2015)
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QPS Austria GmbH has not listed any services.

Biomarker Assay Development
Price on request

QPS Austria’s expertise lies within the field of neurodegenerative diseases. We provide a state of the art research environment (GLP and AAALAC certified) for testing and evaluating new potential treatment approaches.

Besides offering a broad spectrum of transgenic models and applications, we offer the establishment of new and... Show more »

QPS Austria’s expertise lies within the field of neurodegenerative diseases. We provide a state of the art research environment (GLP and AAALAC certified) for testing and evaluating new potential treatment approaches.

Besides offering a broad spectrum of transgenic models and applications, we offer the establishment of new and customized assays that suit your special needs.

Research Fields

  • Alzheimer's Disease
  • Parkinson's Disease
  • Tauopathies
  • Niemann-Pick Disease
  • Inflammation

Applications

  • Gene & Protein Expression
  • Enzyme Activity Assays
  • Customized Cloning
  • Cytokine Profile
  • Lipid Profile
  • A4 Assay
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Statistical Analysis
Price on request
  • Professional statistical data analysis (unbiased and representative) performed by our team of scientists and biostatisticians
  • Conscientious quality checks of measurements and results

Reporting of Experiments and Results

  • At the end of each study our customers receive a full study report including all experimental... Show more »
  • Professional statistical data analysis (unbiased and representative) performed by our team of scientists and biostatisticians
  • Conscientious quality checks of measurements and results

Reporting of Experiments and Results

  • At the end of each study our customers receive a full study report including all experimental procedures, qualitative and quantitative assessment of the results, as well a comprehensive statistical analysis.
  • Constant updates throughout the study if desired.
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Human Biobanking
Price on request

QPS Austria’s well characterized and validated in vivo and in vitro models are useful tools to push your CNS drug discovery research forward.

We are happy to support your research activities with sample material from our biobank composed of various specimen derived from our in-house in vivo and in vitro models.

Various... Show more »

QPS Austria’s well characterized and validated in vivo and in vitro models are useful tools to push your CNS drug discovery research forward.

We are happy to support your research activities with sample material from our biobank composed of various specimen derived from our in-house in vivo and in vitro models.

Various transgenic and non-transgenic in vivo models are readily available on various age groups as well as brain tissue, CSF and plasma samples derived from these animals. In vitro systems e.g. cellular lysates of primary hippocampal neurons and manifold overexpressing cell lines are rapidly available upon request.

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Image Analysis
Price on request

We use sophisticated macro-based software solutions to automatically analyze different features of brain morphology and pathology, e.g. numerical densities of cell somata or axonal fibers, synaptic density, dendritic arborization, area and volume of brain areas or plaques. We are pleased to develop new quantification methods, as... Show more »

We use sophisticated macro-based software solutions to automatically analyze different features of brain morphology and pathology, e.g. numerical densities of cell somata or axonal fibers, synaptic density, dendritic arborization, area and volume of brain areas or plaques. We are pleased to develop new quantification methods, as well as to assess used (feasibility analysis) or ameliorate existing methods (proof of measurement).

Identification and Detection of Labeled Structures

  • Accuracy of detected labeled structures through:
    • Validation of antibodies on knockout tissue whenever possible
    • Appropriate imaging controls
  • Determination and consistent usage of thresholding and filtering parameters throughout the whole study

Analysis of Region and Object Features

We routinely offer a wide variety of automatically generated outcome measures. These include:

  • Total immuno-reactive area
  • Sum or mean fluorescence intensity of immuno-reactive particles
  • Number, size, density and shape of labeled particles
  • Size exclusions and binning of objects
  • Exact morphometric delineation of regions of interest for volume estimation
  • And many more
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Special Slide Staining
Price on request
  • Thioflavin S or Congo Red (amyloid plaques, tau tangles)
  • Golgi-Cox silver impregnation
  • Prussian Blue (microbleedings)
  • Thioflavin S or Congo Red (amyloid plaques, tau tangles)
  • Golgi-Cox silver impregnation
  • Prussian Blue (microbleedings)
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H&E Staining
Hematoxylin and eosin staining
Price on request

We routinely perform both fluorescent and chromogenic immunostainings as well as general histological stainings and stainings for special applications. Additionally, we are happy to establish and optimize customized staining protocols for your specific needs.

Our Expertise Includes:

We routinely perform both fluorescent and chromogenic immunostainings as well as general histological stainings and stainings for special applications. Additionally, we are happy to establish and optimize customized staining protocols for your specific needs.

Our Expertise Includes:

  • Hundreds of established staining protocols
  • Antibody testing and optimization
  • Target validation of viral infections
  • Target validation in transgenic models
  • Compound detection

Multichannel Immunofluorescence

  • 3-channel immunofluorescence plus DAPI labeling of nuclei
  • Numerous validated antibody combinations for mouse, rat and human tissue

General Stainings

  • Hematoxilin-Eosin
  • Cresyl violet (Nissl)
  • Luxol fast blue
  • … and many more
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Tissue Sectioning
Price on request

Our expertise covers a wide range of sectioning techniques and tissue collection strategies. We process your samples according to the specific requirements of your project.

Sectioning

  • Sliding microtome (Leica, SM2000R)
  • Cryotome (Leica, CM1950S and CM3050S)
  • Vibrating blade microtome (Leica, VT1000S)
  • Choose any... Show more »

Our expertise covers a wide range of sectioning techniques and tissue collection strategies. We process your samples according to the specific requirements of your project.

Sectioning

  • Sliding microtome (Leica, SM2000R)
  • Cryotome (Leica, CM1950S and CM3050S)
  • Vibrating blade microtome (Leica, VT1000S)
  • Choose any orientation and specify the section thickness

Collecting

  • Specify your collection scheme
  • Systematic uniform random sampling available
    (For explanation see: http://www.stereology.info/sampling/)
  • Free floating sections
  • Sections mounted on slides
  • Choose slides according to your needs
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Paraffin Embedding
Price on request

Just discuss your needs with us, and we take care of dissecting the target tissue, accurate trimming of the tissue block, fixation and embedding of your tissue samples. Highly standardized default procedures are established, but alternatives can be used upon request. We are experienced working RNase-free.

Fixation

-... Show more »

Just discuss your needs with us, and we take care of dissecting the target tissue, accurate trimming of the tissue block, fixation and embedding of your tissue samples. Highly standardized default procedures are established, but alternatives can be used upon request. We are experienced working RNase-free.

Fixation

  • Standard: 4% paraformaldehyde
  • Alternatives: Glutaraldehyde, zinc, picric acid
  • Buffers: PBS, cacodylate, sulfide

Embedding

  • Standard: OCT or paraffin (Sakura, Tissue-Tek TEC)
  • Alternative: Plastic
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Animal Behavior Studies
Price on request

Corner Turn Test

The Corner Turn Test measures sensorimotor impairments by unilateral damage to the dopaminergic system.

The apparatus consists of two similar walls that are connected in a pointed angle of 30° with a small opening at the tip of the angle so the animal’s curiosity encourages entrance into the corner. Once... Show more »

Corner Turn Test

The Corner Turn Test measures sensorimotor impairments by unilateral damage to the dopaminergic system.

The apparatus consists of two similar walls that are connected in a pointed angle of 30° with a small opening at the tip of the angle so the animal’s curiosity encourages entrance into the corner. Once the animal enters the corner the attempts of turns towards the impaired or non-impaired body side are recorded. Performance of test is videotaped and the number of turns is manually counted.

Corridor Test

The Corridor Test is a sensitive system to measure sensorimotor impairment by unilateral damage to the dopaminergic system.

The apparatus consists of a long narrow corridor with 10 pairs of flanking jars. Each jar contains a sugar pellet. Animals are starved overnight and habituated to the corridor and the sugar pellets. After habituation, sugar pellets are placed in the jars that are positioned at 5cm intervals along the corridor always two jars next to each other. The animal is put in one corner of the corridor and videotaped while eating sugar pellets from the jars. The first 20 nose pokes are manually counted and the proportion between right and left jar are calculated.

Rotation Test

The Rotation Test is a sensitive system to measure sensorimotor impairment by unilateral damage to the dopaminergic system.

The rotometer bowls are hemispheres with a 50 cm diameter. In this apparatus unilaterally lesioned animals are more likely to show rotation behavior compared to e.g. the open field apparatus. Animals are placed in the rotometer bowls and their behavior during the test session is videotaped. The test is performed under standard light illumination.

Behavioral parameters like clockwise/counterclockwise rotations are automatically counted with a tracking system.

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Animal Social Domain Tests
Price on request

The three chamber social test analyzes for social deficits and social recognition in rodents. The test uses the natural social behavior of rodents to prefer company over being alone.

The test apparatus consists of three chambers that are inter-connected. Social approach and social recognition can be measured.

Social... Show more »

The three chamber social test analyzes for social deficits and social recognition in rodents. The test uses the natural social behavior of rodents to prefer company over being alone.

The test apparatus consists of three chambers that are inter-connected. Social approach and social recognition can be measured.

Social Approach: A mouse is placed under a wire cage in the left chamber of the test apparatus. An empty wire cage is placed in the right chamber. The test animal is placed in the middle chamber and it is measured, how long the test mouse spends in each chamber or sniffs at each wire cage. The test is videotaped for 10 minutes. Animals can smell, see and hear each other but direct body contact is not possible. Healthy animals will explore each chamber, but will spend most of the time with the conspecific while socially disturbed mice will spend less time with the conspecific.

Social Recognition: After the social approach test, mice are immediately tested for social recognition. Therefore, the already known mouse stays in the left chamber and a new mouse is placed under the wire cage in the right chamber. The test mouse is again placed in the middle chamber and it is for 10 minutes measured how much time the animal spends in each chamber and spends sniffing at the already known or new mouse. Healthy mice will spend more time with the new conspecific due to novelty. Socially disturbed mice might not recognise the already known conspecific and spend therefore similar times in each chamber or the middle chamber.

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Animal Social Behavior Test Panel
Price on request

Autogrooming Test

Grooming is a normal behavior of healthy mice to clean themselves. It can be distinguished between social grooming (grooming of another mouse) and autogrooming (self grooming). In socially disturbed animals, autogrooming can be strongly increased. This behavior can mostly be observed when animals are housed... Show more »

Autogrooming Test

Grooming is a normal behavior of healthy mice to clean themselves. It can be distinguished between social grooming (grooming of another mouse) and autogrooming (self grooming). In socially disturbed animals, autogrooming can be strongly increased. This behavior can mostly be observed when animals are housed separately.

For this test, animals are therefore housed separately for three days. On the 4th day, animals are placed in the open field box and after a habituation phase of 10 minutes, animals are filmed for another 10 minutes. It is measured, how much time animals spent autogrooming within this 10 minutes.

The Reeperbahn Test

The Reeperbahn Test is a space-learning paradigm in which male subjects have to learn the location of a female in a dry-land apparatus.

Males can freely explore the arena during learning. In the recall sessions, males are placed back in the same environment while females are absent. During the whole test, animals receive no rewards or punishments. In addition to automated behaviour measurements like total locomotion activity, index of learning and recall, also the ultrasound vocalisation of males and ultraviolet urine mark measurements can be performed.

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Anxiety Animal Models
Price on request

Marble burying test

Marble burying is commonly used to test for anxiety, where burying positively correlates with anxiety. The test is also published to analyze for repetitive behavior.

Two days prior to the training, two glass marbles placed into the animal`s home cage to prevent neophobia during testing. Training and... Show more »

Marble burying test

Marble burying is commonly used to test for anxiety, where burying positively correlates with anxiety. The test is also published to analyze for repetitive behavior.

Two days prior to the training, two glass marbles placed into the animal`s home cage to prevent neophobia during testing. Training and testing is conducted in a new home cage (equal size, filled with 3 cm wood chip bedding, covered with a wired cage lid), under dimmed light. On the training day, animals are allowed to freely explore the testing cage without marbles for 30 min for habituation. On the testing day, approx. 24 hours after training, 15 marbles are evenly placed in the test cage to ensure equal and consistent positioning of marbles. Animals are exposed to the marbles for 30 min, while videotaped. The manually evaluated parameters are latency to start burying and number of buried marbles.

Novelty-induced Hypophagia (NIH) Test

The Novelty-induced Hypophagia test is a behavior paradigm sensitive to anxiety and depression-like states.

The single-housed rats are presented in their home cages with a bottle of sweetened milk for 15 min. A habituation phase is performed on 4 consecutive days. On the 4th day, the latency to drink and the amount of milk consumed is measured in the home cage. On the 5th day, animals are tested in a novel cage of same dimensions without bedding and under bright light of approx. 1000 Lux, thus in an aversive environment. The latency to approach the bottle and to start drinking, as well as the amount of milk consumed within 15 minutes is measured. The performance of the animals at the 4th and 5th day is videotaped and manually analyzed.

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Forced Swim Test
Price on request

The Forced Swim Test is based on the assumption that animals try to escape from an aversive stimulus. The test is used to measure the effect of antidepressant drugs or the genotype on the emotional status of animals.

Animals are placed in a transparent Plexiglas cylinder filled with water during test session. The animal’s... Show more »

The Forced Swim Test is based on the assumption that animals try to escape from an aversive stimulus. The test is used to measure the effect of antidepressant drugs or the genotype on the emotional status of animals.

Animals are placed in a transparent Plexiglas cylinder filled with water during test session. The animal’s behavior is recorded and the duration of immobility as well as struggling and swimming is measured. Increased immobility (floating) is interpreted as a behavioral correlate of negative mood, representing a kind of depression in the animal.

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Elevated Plus Maze Animal Anxiety Test
Price on request

The Elevated Plus Maze (EPM) is currently one of the most frequenty used tests to study anxiety in small rodents.

The test is based on the aversion of rodents to open spaces and height. The EPM apparatus is elevated from the floor. The equipment is a four arm maze with two opposing open arms (lighted arms, without walls) and... Show more »

The Elevated Plus Maze (EPM) is currently one of the most frequenty used tests to study anxiety in small rodents.

The test is based on the aversion of rodents to open spaces and height. The EPM apparatus is elevated from the floor. The equipment is a four arm maze with two opposing open arms (lighted arms, without walls) and two opposing closed arms (sheltered arms, with walls). On the test day, the animal is placed in the center area facing the open arm. The behavior during the test session is recorded, and behavioral parameters such as time spent in the open and in the closed arms, number of visits in the open and closed arms as well as latency to enter the open arm are calculated automatically.

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Animal Motor Function and Activity Testing
Price on request

Several customized behavioral tests are offered to phenotype mouse and rat models and evaluate effects of compounds in different in vivo models.

New behavioral tests are continuously developed and validated.

  • Beam Walk and Challenging Beam Walk Test
  • Pasta Gnawing Test
  • Pole Test
  • RotaRod Test
  • Wire Hanging Test

Several customized behavioral tests are offered to phenotype mouse and rat models and evaluate effects of compounds in different in vivo models.

New behavioral tests are continuously developed and validated.

  • Beam Walk and Challenging Beam Walk Test
  • Pasta Gnawing Test
  • Pole Test
  • RotaRod Test
  • Wire Hanging Test
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Cognition and Behavior Animal Models
Price on request

Passive and Active Avoidance Test

The passive avoidance behavior is based on negative reinforcement and examines long-term memory and emotional learning.

In this task, the animals learn to refrain from stepping through a door to an apparently safer but previously punished dark chamber. A light and a dark chamber are... Show more »

Passive and Active Avoidance Test

The passive avoidance behavior is based on negative reinforcement and examines long-term memory and emotional learning.

In this task, the animals learn to refrain from stepping through a door to an apparently safer but previously punished dark chamber. A light and a dark chamber are connected in an apparatus by a guillotine door.

Acquisition: The mouse is placed into the illuminated chamber of the apparatus upon entering the dark chamber, the door will close and an electrical stimulus is applied. Retention test: 24 hours after acquisition, the animal is placed back into the illuminated chamber again. The time until the animal visits the dark chamber (latency) is measured and serves as an index of emotional memory. All parameters are measured using an automated tracking system.

Two Choice Swim Test

An unique feature of this test is the possibility to test spatial learning abilities also in modestly motor-impaired animals, since the choice accuracy is not influenced by motor deficits.

A corridor-like tank is filled with tainted water and illuminated on one end. The hidden platform is positioned either at the dark or lit end of the tank. The mouse is placed in the centre of the tank. During acquisition of the task, each animal performs several training trials by swimming towards or away from the light to find the platform. Following the acquisition phase, the platform is switched towards the opposite end of the corridor, while the light source remains at the former position to test for reversal learning. In all trials, both choice and latency to reach the platform are recorded.

Y-Maze Test

The Y-Maze Test is based on the natural behavior of rodents to explore new environments. Rodents typically prefer to investigate new environments rather than familiar ones-so animals will explore a new arm of the maze rather than returning to one that was previously visited. Many parts of the brain including the hippocampus, septum, basal forebrain, and prefrontal cortex are involved in this task.

Spontaneous Alternation test

Testing occurs in a Y-shaped maze with three plastic arms oriented in a 120° angle. After introduction to the center of the maze, the animal is allowed to freely explore the three arms, where it should show a tendency to enter a less recently visited arm. This test is used to quantify cognitive deficits in transgenic animals and evaluate novel compounds for their effects on cognition.

Y-Maze Novelty and Memory

To test whether rodents prefer to spend time in new or familiar areas, one arm of the Y-maze is blocked and the animal is allowed to explore the other two arms. The rodent is then placed in the start arm and the blocked arm is opened, so the animal should show a tendency to enter the formerly blocked arm more frequently. The Y-maze test is particularly useful as an initial test of memory function in mice.

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Novel Object Recognition Test
Price on request

This two trial test is used to assess memory for interactions with novel objects. Rodents tend to spend more time interacting with a new object rather than with an already known object. Acquisition: after habituation to the test chamber on the first day, the animal is placed in the box and allowed to explore two identical objects... Show more »

This two trial test is used to assess memory for interactions with novel objects. Rodents tend to spend more time interacting with a new object rather than with an already known object. Acquisition: after habituation to the test chamber on the first day, the animal is placed in the box and allowed to explore two identical objects for a set period of time on the second day. Retention: on the third day, the animal is returned to the box where one of the familiar objects has been replaced with a novel object. The time exploring the novel object (relative to the time spent exploring the familiar object) is used as a measure of memory.

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Morris Water Maze Assay
Price on request

The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water, and have to swim to a hidden escape platform. Each mouse has to perform three trials on each of four consecutive days. After the last trial on day 4, mice have to fulfill a probe trial. During the probe trial, the... Show more »

The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water, and have to swim to a hidden escape platform. Each mouse has to perform three trials on each of four consecutive days. After the last trial on day 4, mice have to fulfill a probe trial. During the probe trial, the platform is removed from the pool and the number of crossings over the former target position is recorded together with the abidance in this quadrant.

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Animal Fear Conditioning Studies
Price on request

This test allows the simultaneous assessment of associative learning by simple cues and/or learning by complex stimuli such as context.

Animals are trained and tested on 2 consecutive days. On the training day, animals are placed in a chamber and receive two conditioned stimuli / unconditioned stimuli package (tone/foot shock).... Show more »

This test allows the simultaneous assessment of associative learning by simple cues and/or learning by complex stimuli such as context.

Animals are trained and tested on 2 consecutive days. On the training day, animals are placed in a chamber and receive two conditioned stimuli / unconditioned stimuli package (tone/foot shock). Contextual memory is tested 24 hours after training. The animal is placed into the same chamber as during the training and freezing behavior is automatically recorded. Cued memory is scored one hour after the contextual test in a novel, modified chamber where the animal receives the auditory cued stimulus and freezing behavior is again automatically recorded.

The time spent freezing during the contextual test and tone-dependent test are defined as index of fear-related learning and memory. Cued and contextual fear conditioning is a task that measures the ability of the animal to learn and remember an association between an aversive experience and environmental cues. All parameters of the test are measured automatically with a tracking system.

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Genetically Engineered Mice
Price on request

Tau transgenic mouse model

Experts in the field have debated for decades whether amyloid-beta or Tau is the better target to cure Alzheimer’s Disease. We cannot answer this question but we can offer an extensive CRO service with transgenic mouse models for both aspects of the disease.

The TMHT (Thy-1 Mutated Human Tau)... Show more »

Tau transgenic mouse model

Experts in the field have debated for decades whether amyloid-beta or Tau is the better target to cure Alzheimer’s Disease. We cannot answer this question but we can offer an extensive CRO service with transgenic mouse models for both aspects of the disease.

The TMHT (Thy-1 Mutated Human Tau) mouse was developed in-house and is exclusively available at QPS Austria. These animals represent a suitable model not only for Alzheimer’s Disease but also for other Tauopathies such as Frontotemporal Dementia and Parkinsonism linked to chromosome 17 (FTDP-17) and Niemann Pick’s disease.

Many years of experience with this model gave us the opportunity to extensively test the model in internal validation studies and also in contract research studies for our customers. Various compounds of different classes (among them also antibody treatments) were positively tested for their efficacy in the TMHT model. Published examples are the γ-secretase modulator CHF5074 (Lanzilotta 2010), sodium selenate (Corcoran et al., 2010) and grape-seed polyphenol extract (Wang et al., 2010).

TMHT mice express the longest human Tau isoform Tau441 (2N4R) with two mutations, V337M and R406W, under regulatory control of the neuron specific murine Thy-1 promoter. Mice are bred in our facility on a C57BL/6 background. The human Tau overexpression leads to high levels of soluble and sarcosyl insoluble tau in the brain with an age dependant increase.

Transgenic Tau gets hyperphosphorylated at different disease relevant residues. Tau hyperphosphorylation is accompanied by a pronounced memory deficit. Already 5 months old TMHT animals demonstrate spatial learning deficits as shown by significantly increased swim length and escape latency. Learning curves in MWM are by far not as steep as in non-transgenic littermates.

Importantly, no motor deficits are observed for this model. Further, the TMHT mouse line resembles human AD TAU-pathology as evaluated by immunohistochemistry. Human Tau accumulates mainly in neuronal somata. Especially in the amygdala an age-dependent increase of both pTau and human total Tau levels is observed.

The translational value of preclinical studies in Tau transgenic mouse models to human trials is intensely discussed. Of special interest in that regard, is certainly the Morris water Maze test for memory, since there highly comparable cognitive test exist for humans. Another aspect comes from recently performed histological comparisons of Tau pathology in human AD samples and samples from TMHT mice that revealed remarkable similarities.

QPS Austria offers custom tailored study design for this model and we are flexible to accommodate to your special interest. We are also happy to advice you and propose previously successful study designs. A typical turnaround time from agreement to the study plan to the final report is about 4 months. QPS Austria maintains its own colony directly in our research facility. Animals of all age groups are typically available without any long latency. Compared to other Tau transgenic mouse lines, the TMHT line shows relevant features of Tauopathies already at young age. This allows for extraordinarily fast turn-around times. Further direct non-transgenic littermates are available as control animals needed for proper study design.

We would be happy to test your compounds in our Tau transgenic mouse model! The most common readouts are:

  • Memory (MWM)
  • Tau and Tau phosphorylation (p181, p202, p231, p262, and p396) in brain extracts
  • Tau pathology evaluated by IHC with antibodies HT7(human Tau), Tau-5(total Tau), AT180, 9G3
  • Looking for something else? Please contact us!

Alpha-Synuclein transgenic Mouse Models

QPS Austria has a license to Patent Rights owned by the Regents of the University of California for providing commercial testing services with several human alpha-synuclein transgenic mouse lines developed by Dr. Eliezer Masliah of UCSD. The three alpha-synuclein transgenic mouse lines available at QPS Austria feature different properties with regard to cellular expression patterns of human alpha-synuclein, age at onset and progression of pathology, and the isoform of human alpha-synuclein being expressed (wildtype or A53T-mutated). These animals constitute suitable models to study the influence of drugs on alpha-synuclein-related brain pathology and behavior. Cellular accumulation of alpha-synuclein is a common histopathological finding in Parkinson’s disease (PD).

PD is a neurodegenerative disease characterized by loss of dopaminergic neurons in the mesencephalon and by progressing reduction of dopaminergic innervation of the dorsal striatum. Symptoms of PD are impairments of motor and cognitive functions, and vegetative disturbances. Among other findings, PD is associated with increased ubiquitination and with the accumulation of alpha-synuclein in Lewy bodies and dystrophic neurites.

Some mutations of alpha-synuclein have been identified that increase the risk for familial PD. These findings have led to the generation of several transgenic mouse models that express different isoforms of human alpha-synuclein under control of different promoters, in order to investigate pathological changes and test the efficacy of pharmaceutical compounds in preclinical drug development studies in vivo. Consequently, interference with aggregation of alpha-synuclein is a common target in efforts of developing pharmaceutical approaches to prevent or ameliorate PD.

R6/2 transgenic Mice

Huntington’s disease (HD) is a neurodegenerative genetic disorder that affects muscle coordination and leads to cognitive decline and dementia. It typically becomes noticeable in middle age. HD is the most common genetic cause of abnormal involuntary writhing movements called chorea. The disease is caused by an autosomal dominant mutation of the huntingtin gene.

The mutation of the huntingtin gene codes for a different form of the huntingtin protein, whose presence results in gradual damage of specific areas of the brain. Symptoms of the disease can vary between individuals and among affected members of the same family, but they progress predictably for most individuals. The earliest symptoms are a general lack of coordination and an unsteady gait.

As the disease advances, uncoordinated, jerky body movements become more apparent, along with a decline in mental abilities and behavioral and psychiatric problems. Physical abilities are gradually impeded until coordinated movement becomes very difficult. Mental abilities generally decline into dementia.

Transgenic mice (R6/2 strain) have been developed to model human Huntington’s disease (HD) by expression of a portion of the human HD gene under human gene promoter elements (1 kb of 5 UTR sequence and exon 1 together with ~140 CAG repeats). Expression of this amino-terminal fragment of the huntingtin protein with its polyglutamine expansion is sufficient to reproduce the phenotype of human HD.

SOD1-G93A tg Mouse Model

SOD1-G93A mice express human SOD1 with the G93A mutation under control of the cistronic human SOD1 promotor. The Super Oxide Dismutase (SOD1) binds free copper and zinc ions and destroys free superoxide radicals in the body. Mutations in this gene have been linked to familial Amyotrophic Lateral Sclerosis (ALS or Lou Gehrig’s disease).The SOD1-G93A mice show a phenotype similar to Amyotrophic Lateral Sclerosis in humans. They develop paralysis in one or more limbs within a few months.

These mice are a valuable tool to study the influence of new drugs on neuromuscular disorders such as Amyotrophic Lateral Sclerosis.

TDP-43 transgenic Mouse Model

TDP-43 related neurodegeneration has gained more and more attention during the last years or research. TDP-43 mouse models are very new on the market and QPS Austria is proud to be in first line to offer CRO services with such a transgenic mouse model.

The human TDP transgenic mouse model, the Tar6/6 (overexpressing unmutated human TARDBP under Thy1 promoter) mouse, is a suitable model to study important pathological cross-links in a growing set of neurodegenerative diseases. While strongly related to sporadic and familial forms of Amyotrophic Lateral Sclerosis (ALS), intraneuronal pTDP-43 accumulation and aggregation is also related to Fronto-temporal Lobe Degeneration (FTLD-TDP or formerly FTLD-U) and was recently shown to be correlated to Alzheimer’s Disease (AD) pathology and Hippocampal Sclerosis.

The Tar6/6 mouse was developed by Wils et al. 2010 and represents one of QPS Austria’s newest models. Behavioral and basic histological characterization is recently finished and revealed fundamental new findings, making it a model of high interest for drug development at the top of new research.

Tar6/6 mice express human Tar DNA binding protein (TARDBP) under regulatory control of the neuron specific Thy1 promoter. Mice are bred in our facility on a C57BL/6 background. Homozygous mice die prematurely with and approximate 50% survival at 6 months and suffer from a severe ALS like motor phenotype. They are unable to fulfill the wire suspension task at an age of 6 weeks, and this early disability is predictive for the homozygous genotype. They further develop deficits on running wheel task, disturbed nest building, and altered anxiety levels. At approximately three months of age they display memory deficits in contextual fear conditioning.

All those phenotypical changes are accompanied by severe neurodegeneration seen in the brain, especially in thalamic neurons of these mice. Most central thalamic nuclei suffer from a severe break down of dendritic network, which is followed by neuronal loss and a strong local neuroinflammation. The latter as seen by astro- and microgliosis can also be found in the hypothalamus, the medulla oblongata and to a lower extent in spinal cord grey matter. The special vulnerability of thalamic neurons to aggregating TDP-43 related to motor disabilities is a brand new and exciting finding. Also heterozygous mice display alterations in the thalamic dendritic network, although to developing an early motor phenotype. In how far ageing might lead to a slower version of the modeled disease is actually under investigation.

Hence these mice offer a unique opportunity to counteract TDP-43 related neurodegeneration in a fast and severe motor and memory phenotype model.

QPS Austria offers custom tailored study design for this model and we are flexible to accommodate to your special interest. We are also happy to advice you and propose successful study designs. A typical turnaround time from agreement to the study plan to the final report is about 4 months. QPS Austria maintains its own colony directly in our research facility. Animals of all age groups are typically available without any long latency. Direct, non-transgenic littermates are available as control animals needed for proper study design.

We would be happy to test your compounds in this new exciting mouse model! The most common readouts are:

  • Memory (CFC), Motor behavior (RotaRod, Beam Walk, Wire Hanging, Nest Building)
  • TARDBP expression, pTDP-43
  • Dendritic network break down and neuronal loss
  • Gliosis (astro- and activated microglia)
  • Looking for something else? Please contact us!
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Alzheimer's Disease Animal Models
Price on request

Alzheimer’s disease (AD) is one of the most devastating neurodegenerative diseases of the 21st century. A disturbed APP metabolism (e.g. pathological aggregation of amyloid) in the brain of AD patients is thought to be one of the main causes for the observed progressive cognitive decline in affected people. The development of new... Show more »

Alzheimer’s disease (AD) is one of the most devastating neurodegenerative diseases of the 21st century. A disturbed APP metabolism (e.g. pathological aggregation of amyloid) in the brain of AD patients is thought to be one of the main causes for the observed progressive cognitive decline in affected people. The development of new AD targeting APP related mechanisms is therefore one main focus in AD research. To be able to test these new drugs, appropriate animal models are needed.

QPS Austria currently offers seven human APP transgenic mouse lines featuring different properties with regard to Aβ expression patterns, neuroinflammation, cognitive deficits, age at onset and progression of pathology. These animals focus on different pathological readouts and constitute suitable models to study the influence of drugs on APP-related brain pathology and behavior.

APPSL transgenic Mouse Model
APPSL x hQC transgenic Mouse Model
ApoB x APP transgenic Mouse Model
5xFAD transgenic Mouse Model
TBA2.1 transgenic Mouse Model
Tg4-42 (TBA83) Mouse Model
Tg2576 Mouse Model

QPS Austria offers custom tailored study designs for these models and we are flexible to customize to your special need. We are also happy to advise you and propose previously successful study designs. A typical turnaround time from agreement to the study plan to the final report is about 4 months. QPS Austria maintains its own colonies directly in our research facility, and animals of all age groups are typically available without any long latency. This allows for extraordinarily fast turn-around times. Non-transgenic control littermates are available as needed for proper study design. We would be happy to test your compounds in our APP transgenic mouse models! The most common readouts are:

  • Soluble and insoluble Aβ levels
  • Aβ Oligomers
  • APP plaques
  • LOC
  • Thioflavin S
  • Neuroinflammation
  • Cognition
  • Looking for something else? Please contact us!
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Neuronal Assays
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During development, neurons become assembled into functional networks by growing out axons and dendrites (collectively neurite outgrowth) that connect synaptically to other neurons. Strikingly, neurons retain their capacity for growth and synapse formation in the adult brain. Only those neurons that manage to establish adequate... Show more »

During development, neurons become assembled into functional networks by growing out axons and dendrites (collectively neurite outgrowth) that connect synaptically to other neurons. Strikingly, neurons retain their capacity for growth and synapse formation in the adult brain. Only those neurons that manage to establish adequate contacts with neighbor cells escape cell death. To stimulate and accelerate neurite outgrowth is thus important for restoring neuronal function after injury or from neurodegenerative diseases. A promising strategy for drugs targeted against neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease is promoting the regeneration of neurons. Thus, new therapies are focusing on identifying new molecules that affect neurite outgrowth. For this purpose, QPS Austria provides cell culture models to assess the neurotrophic effects of your developmental compounds.

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Neurogenesis
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The balance between neurogenesis and cell death plays a critical role not only in the embryonic brain development but also in the maintenance of the adult brain. Alterations in these processes are seen in many neurodegenerative diseases. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to... Show more »

The balance between neurogenesis and cell death plays a critical role not only in the embryonic brain development but also in the maintenance of the adult brain. Alterations in these processes are seen in many neurodegenerative diseases. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to damage at early stages of Alzheimer’s disease (AD). Emerging evidence has indicated that compromised neurogenesis in the adult hippocampus represents an early critical event in the course of AD. For this purpose, QPS Austria provides a cell culture model to assess effects of your developmental compound on neurogenesis of hippocampal neurons.

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Parkinsons Disease Animal Models
Price on request

Parkinson’s disease (PD) is a slowly progressive neurodegenerative disease clinically characterized by progressive motor impairment in affected people. Synaptic and axonal degeneration is followed by loss of dopaminergic neurons in the substantia nigra leads to reduced levels of dopamine in the nigrostriatal circuitry. Besides... Show more »

Parkinson’s disease (PD) is a slowly progressive neurodegenerative disease clinically characterized by progressive motor impairment in affected people. Synaptic and axonal degeneration is followed by loss of dopaminergic neurons in the substantia nigra leads to reduced levels of dopamine in the nigrostriatal circuitry. Besides dopaminergic cell loss, intracellular formation of Lewy bodies, consisting predominantly of aggregated alpha-synuclein, has been suggested to be crucial in the pathogenesis of this disease.

PD is a complex, multifactorial disease in which different factors concur to the pathogenic process. In vitro models (established cell lines, primary cell cultures or lesion models) offer the advantage of a controlled environment favorable for exploring single pathogenic mechanisms and the genes/proteins involved.

On the cellular level, research in PD focuses on:

  • Neurotoxicity (MPP+, 6-OHDA, BSO,…)
  • Excitotoxicity (NMDA, glutamate,…)
  • Mitochondrial dysfunction
  • Defects in protein degradation

QPS Austria offers several cellular solutions to model PD pathology in vitro:

  • Primary TH neurons
  • Primary cortical and hippocampal neurons
  • Primary human fibroblasts
  • SHSY-5Y cells overexpressing wt or A53T mutated synuclein
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Alzheimer's Disease Animal Models
Price on request

Alzheimer’s disease (AD) is one of the most devastating neurodegenerative diseases. Aggregation of amyloid-beta peptide (Aβ) into cytotoxic oligomers and fibrills is one of the major hallmarks in AD. These pathological depositions in the brain are thought to be one of the main causes for the observed progressive cognitive... Show more »

Alzheimer’s disease (AD) is one of the most devastating neurodegenerative diseases. Aggregation of amyloid-beta peptide (Aβ) into cytotoxic oligomers and fibrills is one of the major hallmarks in AD. These pathological depositions in the brain are thought to be one of the main causes for the observed progressive cognitive decline in AD patients.

Interfering with Aβ aggregation is an inevitable strategy in the development of novel therapeutic approaches. Reliable in vitro models are needed, which are capable of showing direct effects of compounds on Aβ oligomer formation and thus, a beneficial impact on cell viability.

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Western Blot
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  • Synaptic proteins (glutamate receptor 1, NMDA receptors, synaptophysin, drebrin) e.g. from matured hippocampal neurons
  • Tau and phospho-Tau isoforms
  • Abeta peptide profile, Abeta aggregation assays
  • Cell signaling molecules
  • TDP43
  • Synuclein
  • Synaptic proteins (glutamate receptor 1, NMDA receptors, synaptophysin, drebrin) e.g. from matured hippocampal neurons
  • Tau and phospho-Tau isoforms
  • Abeta peptide profile, Abeta aggregation assays
  • Cell signaling molecules
  • TDP43
  • Synuclein
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Immunofluorescence
Price on request
  • Neuronal markers (beta-III tubulin, neurofilament, MAP2, tyrosine hydroxylase)‏
  • Glial markers (GFAP, CD11b)‏
  • Disease related markers (Tau, phosphorylated Tau, signaling molecules, synuclein)‏
  • Synaptic proteins (synaptophysin)‏
  • Neuronal markers (beta-III tubulin, neurofilament, MAP2, tyrosine hydroxylase)‏
  • Glial markers (GFAP, CD11b)‏
  • Disease related markers (Tau, phosphorylated Tau, signaling molecules, synuclein)‏
  • Synaptic proteins (synaptophysin)‏
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ELISA
Enzyme-linked immunosorbent assay
Price on request
  • Abeta1-38, Abeta1-40, Abeta1-42, sAPP-alpha, sAPP-beta
  • Tau and phospho Tau profile
  • Signal transduction molecules
  • Inflammation markers (e.g. Prostaglandin E2)
  • Cytokine Profile
  • Abeta1-38, Abeta1-40, Abeta1-42, sAPP-alpha, sAPP-beta
  • Tau and phospho Tau profile
  • Signal transduction molecules
  • Inflammation markers (e.g. Prostaglandin E2)
  • Cytokine Profile
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Apoptosis Assays
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Necrosis Assays
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LDH Release Assay
Lactate Dehydrogenase Release Assay
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MTT and MTS Assays
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Barnes Maze Test
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The Barnes maze is a standard behavioral tool to investigate spatial learning and cognition in rodents.

The test consists of a circular arena with equally distributed holes on its periphery with one hole serving as target zone that is equipped with the animal’s home cage. The animal can use proximal or distal visual cues to... Show more »

The Barnes maze is a standard behavioral tool to investigate spatial learning and cognition in rodents.

The test consists of a circular arena with equally distributed holes on its periphery with one hole serving as target zone that is equipped with the animal’s home cage. The animal can use proximal or distal visual cues to locate and remember the target zone. Automated recordings of consecutive trials provide details on the rodent’s navigation in the arena and thus delivers information about their learning and memory performance.

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Synaptogenesis Studies
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Synapse formation but especially the synaptic maintenance (synaptogenesis) is critical for neuronal health and disease. Loss of synaptic maintenance is not only restricted to AD. Defects in synaptic maintenance most likely also contribute to the synaptic vulnerability in other neurodegenerative disorders as Huntington’s,... Show more »

Synapse formation but especially the synaptic maintenance (synaptogenesis) is critical for neuronal health and disease. Loss of synaptic maintenance is not only restricted to AD. Defects in synaptic maintenance most likely also contribute to the synaptic vulnerability in other neurodegenerative disorders as Huntington’s, Parkinson’s or Niemann Pick disease. A therapeutic strategy that favors synaptic maintenance may be a beneficial target for many neurodegenerative diseases. For this purpose, QPS Austria provides a cell culture model to assess effects of your developmental compound on synaptogenesis primary hippocampal neurons.

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Lesion-Induced CNS Animal Models
Price on request

QPS Austria offers several cellular models comprising primary neurons or transgenic and non-transgenic cell lines to mimic disease related conditions. Cellular models offer the advantage of a controlled environment useful for exploring single pathogenic mechanisms and the proteins involved. Our models are fast and cost-efficient.... Show more »

QPS Austria offers several cellular models comprising primary neurons or transgenic and non-transgenic cell lines to mimic disease related conditions. Cellular models offer the advantage of a controlled environment useful for exploring single pathogenic mechanisms and the proteins involved. Our models are fast and cost-efficient. Customized models are generated gladly on request.

  • Abeta1-42
  • MPP+
  • 6-OHDA
  • BSO
  • Synuclein
  • H2O2
  • Ionomycin
  • Okadaic acid
  • NMDA lesion
  • Glutamate lesion
  • Oxygen-glucose deprivation (OGD) in primary neurons
  • Iodoacetate
  • Sodium cyanide
  • Growth factor withdrawal
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Cells and Tissues
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Cell-Based Assays
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Toxicology
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Animal Learning, Memory, and Behavior Tests
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Clinical and Anatomic Pathology
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Genetic Engineering
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Tissue Embedding
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Drug Discovery & Development
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Protein Quantification
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Animal Model in vivo Analyses
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Computational Modeling
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Common Histochemical Stains
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Imaging & Spectroscopy
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Biomarkers
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Animal Anxiety and Depression Tests
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Animal Models of Disease
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Transgenic Mouse Services
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Biology
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Animal Cognition & Behavior Tests
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Primary Cell Culture
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In vitro Neurotoxicity Testing
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Microscopy
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Cytotoxicity Assays
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Drug Development
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Pharmacology & Toxicology
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Biospecimens
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CNS/Neurology Animal Models
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Histological Staining
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Tissue Processing
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Protein Services
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Transgenic Animal Model Development
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Protein Expression Visualization
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Animal Models and Studies
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In vitro Toxicity Testing
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Cognitive, Behavioral, and Psychiatric Animal Models
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Bioanalysis
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Functional & Cell Type Specific Assays
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Immunostaining
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Cell Death Assays
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Biochemistry & Molecular Biology
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Neuroscience
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Clinical Research
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Clinical Laboratory Services
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Data Services
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Animal Motor Function Tests
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Biospecimen Management and Storage
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Animal Social Behavior Tests
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2018-01-08 11:27:49 -0600

Net Promoter Score of 10 received for Immunofluorescence.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-09-22 14:14:58 -0500

Net Promoter Score of 10 received for ELISA.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

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